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1.
Front Public Health ; 11: 1050511, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36741955

RESUMO

Background/aim: Limited research has been conducted regarding the association between mental illness and dental caries. We studied the impact of emotional distress on current and new dental caries among low-income African-American caregivers in Detroit, Michigan and if this association was mediated by poor oral hygiene and sugar consumption and modified by a chronic health condition. Methods: Data came from Detroit Dental Health Project, a prospective cohort study of low-income African American caregivers and their children. We focused on baseline (n = 1,021) and 4-year follow-up participants (n = 614). Dental caries were assessed using the International Caries Detection and Assessment System. The study outcomes included two baseline caries outcomes (counts of non-cavitated lesions, baseline counts of cavitated lesions) and two outcomes of new caries over 4 years (new cavitated lesions and new non-cavitated lesions). The exposure was emotional distress. We performed multivariable quasi-Poisson regression analysis to test the association between emotional distress and caries. We tested effect modification by stratifying data by chronic health conditions and performed causal mediation analysis to test an indirect effect of oral hygiene and sugar consumption. Results: Ninety six percent of the caregivers were female, and their average age was 28 years old. Thirteen percent reported emotional distress at baseline. After accounting for potential confounding, emotional distress was positively associated with cavitated lesions at baseline (IRR = 1.36, 95% CI = 1.08, 1.70). Among those with a chronic health condition, stronger association was observed (IRR = 1.73, 95% CI = 1.27, 2.35). After 4 years, those with emotional distress and chronic health conditions had an increased risk of developing non-cavitated carious lesions (IRR = 1.41, 95% CI = 1.06, 1.88). Poor oral hygiene explained 51% of the association between emotional distress and baseline cavitated lesions (natural indirect effect = 1.16, 95% CI = 1.02, 1.33), but there was no evidence for an indirect effect of sugar consumption. Conclusion: In this group of young, African-American caregivers with low socioeconomic status, dental caries was associated with emotional distress. This association was explained by poor oral hygiene and strengthened among those who reported a chronic health condition.


Assuntos
Cárie Dentária , Angústia Psicológica , Criança , Humanos , Feminino , Adulto , Masculino , Negro ou Afro-Americano , Michigan/epidemiologia , Cuidadores , Cárie Dentária/epidemiologia , Estudos Prospectivos , Açúcares da Dieta
2.
Methods Inf Med ; 61(S 02): e125-e133, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36413995

RESUMO

OBJECTIVE: Our objective was to phenotype periodontal disease (PD) diagnoses from three different sections (diagnosis codes, clinical notes, and periodontal charting) of the electronic dental records (EDR) by developing two automated computer algorithms. METHODS: We conducted a retrospective study using EDR data of patients (n = 27,138) who received care at Temple University Maurice H. Kornberg School of Dentistry from January 1, 2017 to August 31, 2021. We determined the completeness of patient demographics, periodontal charting, and PD diagnoses information in the EDR. Next, we developed two automated computer algorithms to automatically diagnose patients' PD statuses from clinical notes and periodontal charting data. Last, we phenotyped PD diagnoses using automated computer algorithms and reported the improved completeness of diagnosis. RESULTS: The completeness of PD diagnosis from the EDR was as follows: periodontal diagnosis codes 36% (n = 9,834), diagnoses in clinical notes 18% (n = 4,867), and charting information 80% (n = 21,710). After phenotyping, the completeness of PD diagnoses improved to 100%. Eleven percent of patients had healthy periodontium, 43% were with gingivitis, 3% with stage I, 36% with stage II, and 7% with stage III/IV periodontitis. CONCLUSIONS: We successfully developed, tested, and deployed two automated algorithms on big EDR datasets to improve the completeness of PD diagnoses. After phenotyping, EDR provided 100% completeness of PD diagnoses of 27,138 unique patients for research purposes. This approach is recommended for use in other large databases for the evaluation of their EDR data quality and for phenotyping PD diagnoses and other relevant variables.


Assuntos
Registros Odontológicos , Doenças Periodontais , Humanos , Estudos Retrospectivos , Doenças Periodontais/diagnóstico , Computadores , Algoritmos , Fenótipo
3.
Front Artif Intell ; 5: 979525, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36311550

RESUMO

Despite advances in periodontal disease (PD) research and periodontal treatments, 42% of the US population suffer from periodontitis. PD can be prevented if high-risk patients are identified early to provide preventive care. Prediction models can help assess risk for PD before initiation and progression; nevertheless, utilization of existing PD prediction models is seldom because of their suboptimal performance. This study aims to develop and test the PD prediction model using machine learning (ML) and electronic dental record (EDR) data that could provide large sample sizes and up-to-date information. A cohort of 27,138 dental patients and grouped PD diagnoses into: healthy control, mild PD, and severe PD was generated. The ML model (XGBoost) was trained (80% training data) and tested (20% testing data) with a total of 74 features extracted from the EDR. We used a five-fold cross-validation strategy to identify the optimal hyperparameters of the model for this one-vs.-all multi-class classification task. Our prediction model differentiated healthy patients vs. mild PD cases and mild PD vs. severe PD cases with an average area under the curve of 0.72. New associations and features compared to existing models were identified that include patient-level factors such as patient anxiety, chewing problems, speaking trouble, teeth grinding, alcohol consumption, injury to teeth, presence of removable partial dentures, self-image, recreational drugs (Heroin and Marijuana), medications affecting periodontium, and medical conditions such as osteoporosis, cancer, neurological conditions, infectious diseases, endocrine conditions, cardiovascular diseases, and gastroenterology conditions. This pilot study demonstrated promising results in predicting the risk of PD using ML and EDR data. The model may provide new information to the clinicians about the PD risks and the factors responsible for the disease progression to take preventive approaches. Further studies are warned to evaluate the prediction model's performance on the external dataset and determine its usability in clinical settings.

4.
AMIA Annu Symp Proc ; 2022: 846-855, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-37128438

RESUMO

Periodontal disease (PD) is one of the most prevalent dental diseases. Fortunately, it can be prevented if identified early, especially for high-risk patients. Dental electronic health records (EHRs) could help develop a data-driven personalized prediction model using advanced machine learning development of clinical decision support system (CDSS) as in our Phase I, II AMIA-AI showcase. In phase II, we created a CDSS, the Perio-Risk Scoring system (PRSS), to help clinicians generate perio-scores and diagnoses and identify the influential factors. In Phase III (this study), we implemented and compared the patient's risk factors information in five periodontal risk assessment tools [periodontal risk assessment (PRA), PreViser, Sonicare, Cigna, and Periodontal Risk Scoring System (PRSS)]. We examined 1) agreement between the risk scores provided by each of the five risk assessment tools of 20 patients' information and 2) compare the risk scores provided by each tool to the original outcomes (five years outcomes). Fleiss Kappa, Cohen's Kappa, and percentage agreements were performed to determine the agreements between risk scores and original outcomes. We found a -1.24 Kappa value which indicates disagreement between the risk scores provided by five risk assessment tools. Compared to the original outcomes (five-year disease outcomes), PRSS provided the most accurate prediction (70%), followed by Previser (55%), PRA (35%), Phillips (35%), and Cigna (25%). We conclude that using advanced state-of-the-art informatics methods could help us utilize EHR data optimally to represent the current patient populations and their risk factors to provide the most accurate disease risk score. This may promote preventive strategies at the chairside, hoping to reduce PD prevalence, improve quality of life, and reduce healthcare costs.


Assuntos
Sistemas de Apoio a Decisões Clínicas , Doenças Periodontais , Humanos , Qualidade de Vida , Medição de Risco , Inteligência Artificial
5.
J Evid Based Dent Pract ; 21(2): 101571, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34391561

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Silva MCPMD, Lyra MCA, Almeida HCR, Alencar Filho AV, Heimer MV, Rosenblatt A. Caries experience in children and adolescents with Down Syndrome: A systematic review and meta-analysis. Arch Oral Biol. 2020 Jul;115:104,715. doi:10.1016/j.archoralbio.2020.104715. Epub 2020 Apr 8. PMID: 32,422,361. SOURCE OF FUNDING: The Hungarian Human Resources Development Operational Program, the Higher Education Excellence Program of the Hungarian Ministry of Human Capacities to the Therapy Research Module of Semmelweis University, National Research, Development and Innovation Office, Hungarian Scientific Research Fund and the Economic Development and Innovation Operative Programme Grant. TYPE OF STUDY/DESIGN: Systematic review with meta-analysis.


Assuntos
Cárie Dentária , Síndrome de Down , Adolescente , Criança , Cárie Dentária/epidemiologia , Humanos , Prevalência , Desenvolvimento de Programas , Universidades
6.
In Silico Pharmacol ; 9(1): 33, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33936929

RESUMO

Class B1 metallo-ß-lactamases (MBLs) are metalloenzymes found in drug resistant bacteria. The enzyme requires zinc ions, along with conserved amino acid coordination for nucleophilic attack of the lactam ring to induce hydrolysis and inactivation of ß-lactam and some carbapenem antibiotics. To this date there are no clinically relevant class B1 MBL inhibitors, however L-captopril has shown significant results against NDM-1, the most difficult MBL to inhibit. Herein, we report the synthesis and evaluation of novel nucleoside analogues modified with polyethylene glycolamino (PEGA) as potential inhibitors for class B1 MBLs. Molecular dynamics simulations, using internal coordinate mechanics (ICM) algorithm, were performed on subclass B1 enzyme complex models screened with twenty-one possible PEGA-nucleosides. Analogue A, 3'-deoxy-3'-(2-(2-hydroxyethoxy)ethanamino)-ß-D-xylofuranosyluracil showed superior binding, with high specificity to the conserved zinc ions in the class B1 MBL active site by utilizing key ß-lactam mimic points in the uridine nucleobase. The PEGA moiety showed chelating activity with zinc and disrupted the metal-binding amino acid geometry. In all subclass B1 proteins tested, analogue A had the most effective inhibition when compared to penicillin or L-captopril. Chemical synthesis was performed by condensation of the corresponding keto ribonucleoside with PEGA, followed by enantioselective reduction of the formed imine to produce the amino derivative with desired configuration. Pharmacokinetic and pharmacodynamic screenings revealed that PEGA-pyrimidine nucleosides are not toxic, nor violate Lipinski's rules. These results suggested that analogue A can be proposed as a potential metalloenzyme inhibitor against the widespread antibiotic resistant bacteria and is worth further in vitro and in vivo investigations.

7.
BMC Oral Health ; 21(1): 52, 2021 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-33546674

RESUMO

BACKGROUND: Transmission of COVID-19 via salivary aerosol particles generated when using handpieces or ultrasonic scalers is a major concern during the COVID-19 pandemic. The aim of this study was to assess the spread of dental aerosols on patients and dental providers during aerosol-generating dental procedures. METHODS: This pilot study was conducted with one volunteer. A dental unit used at the dental school for general dental care was the site of the experiment. Before the study, three measurement meters (DustTrak 8534, PTrak 8525 and AeroTrak 9306) were used to measure the ambient distribution of particles in the ambient air surrounding the dental chair. The volunteer wore a bouffant, goggles, and shoe covers and was seated in the dental chair in supine position, and covered with a surgical drape. The dentist and dental assistant donned bouffant, goggles, face shields, N95 masks, surgical gowns and shoe covers. The simulation was conducted by using a high-speed handpiece with a diamond bur operating in the oral cavity for 6 min without touching the teeth. A new set of measurement was obtained while using an ultrasonic scaler to clean all teeth of the volunteer. For both aerosol generating procedures, the aerosol particles were measured with the use of saliva ejector (SE) and high-speed suction (HSS) followed a separate set of measurement with the additional use of an extra oral high-volume suction (HVS) unit that was placed close to the mouth to capture the aerosol in addition to SE and HSS. The distribution of the air particles, including the size and concentration of aerosols, was measured around the patient, dentist, dental assistant, 3 feet above the patient, and the floor. RESULTS: Four locations were identified with elevated aerosol levels compared to the baseline, including the chest of the dentist, the chest of patient, the chest of assistant and 3 feet above the patient. The use of additional extra oral high volume suction reduced aerosol to or below the baseline level. CONCLUSIONS: The increase of the level of aerosol with size less than 10 µm was minimal during dental procedures when using SE and HSS. Use of HVS further reduced aerosol levels below the ambient levels.


Assuntos
COVID-19 , Pandemias , Aerossóis , Humanos , Projetos Piloto , SARS-CoV-2 , Saliva , Faculdades de Odontologia
8.
J Dent Educ ; 85(5): 652-659, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33368251

RESUMO

PURPOSE: The transition from a paper-based to an all-electronic patient health record took a major step forward in 2018, with the implementation of an electronic health record that supports the School's patient-centered comprehensive care model and facilitates outcomes assessment. The Patient Wellness Report (PWR) summarizes findings of the patient assessment, and it does so automatically by locating data already entered in axiUm forms. This study aimed to describe the PWR implementation procedures and to examine outcomes and characteristics among patients with completed treatment plans during an 18-month period. METHODS: Outcome data were extracted from axiUm for patients aged ≥16 years who completed comprehensive care treatment plans. Each PWR contained 14 metrics related to "dimensions" of wellness (quality of life, general health factors, oral hygiene, caries risk, and degree of periodontal inflammation and pocketing), each of which is rated on a 3-point scale based on best available scientific evidence. RESULTS: A total of 2074 patients completed planned procedures between July 2018 and January 2020, and met the study eligibility criteria. Improvement of several conditions was observed between baseline and follow-up in caries lesions (21%), blood pressure (9%), and periodontal pocket (3.2%). A majority of patients rated in good condition at baseline had their scores unchanged at follow-up in the following areas: dental anxiety (92%), speaking (88%), smoking (87%), and alcohol consumption (79%). CONCLUSION: Improvements in dental caries and blood pressure metrics were easily monitored using the PWR. In addition, disparities exist in improvement of patient outcomes by race/ethnicity.


Assuntos
Cárie Dentária , Idoso , Cárie Dentária/terapia , Registros Eletrônicos de Saúde , Humanos , Higiene Bucal , Qualidade de Vida , Universidades
9.
Gerodontology ; 37(3): 279-287, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32643211

RESUMO

OBJECTIVES: We conducted a trial to assess the treatment fidelity of an individual-based oral health education intervention utilising motivational interviewing (MI) techniques and its efficacy when compared to a group-based traditional oral health education intervention (TOHE) and a standard of care group (SC) in a sample from Philadelphia during a 12-month follow-up. BACKGROUND: There is lack of information on how different types of oral health educational interventions affect older adults on non-clinical outcomes including changes in oral health-related quality of life (OHRQoL), oral health self-efficacy (SE) and oral health knowledge (OHK). MATERIALS AND METHODS: One hundred and eighty patients were randomly allocated to TOHE, MI and SC groups. Treatment fidelity was measured in 16 non-study patients. The MI intervention was administered by a public health dental hygienist (PHDH). All interviews were audio-recorded and coded by an expert using the Motivational Interviewing Treatment Integrity (MITI) Code. Multivariable longitudinal regression analyses accounting for baseline demographics and correlated errors due to repeated measures via generalised estimating equation were conducted following an intention to treat approach. RESULTS: Over the 1-year follow-up, SE and OHRQoL scores significantly improved amongst the MI group whereas both outcomes worsened amongst the SC group. During the same period, SE and OHRQoL did not change in the TOHE group. CONCLUSION: Findings from the study support the fidelity of this intervention and the improvement of all non-clinical outcomes after 12 months amongst the MI group.


Assuntos
Entrevista Motivacional , Idoso , Educação em Saúde Bucal , Humanos , Saúde Bucal , Philadelphia , Qualidade de Vida
10.
J Evid Based Dent Pract ; 20(1): 101409, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32381411

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Caries reporting in studies that used the International Caries Detection and Assessment System: A scoping review. ElSalhy M, Ali U, Lai H, Flores-Mir C, Amin M. Community Dent Oral Epidemiol 2019; 47(1): 92-102. SOURCE OF FUNDING: Alberta Innovates-Health Solutions Grant Award Number RES 0027148 and The Honorary Izaak Walton Killam Memorial Scholarship. TYPE OF STUDY/DESIGN: Comprehensive literature review (a scoping review).


Assuntos
Cárie Dentária , Humanos
11.
J Public Health Dent ; 80(2): 168-174, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32285476

RESUMO

OBJECTIVE: To propose an individual-level indicator of caries severity based on the maximum score generated using the international caries detection and assessment system (ICDAS) and test validity of this individual-level indicator by assessing differences between this proposed indicator and the traditional decayed, missing, and filled surfaces (DMFS or dmfs for primary teeth) index. METHODS: Data on caries severity were collected using a representative sample of children from Detroit, Michigan, aged less than 6 years (N = 749) examined in 2002-2003 (W1) and 2004-2005 (W2). For each wave, each child was assigned to six caries severity groups based on the maximum ICDAS scores. This individual-level indicator was then compared with the surface-level dental caries measure (dmfs + DMFS). In addition, caries progression between W1 and W2 were assessed using individual-level and tooth surface-level indicators. RESULTS: Both measures were linearly related, and the relationship was statistically significant (P < 0.001). CONCLUSION: An individual-level caries progression indicator can be developed that is simple and has the ability to communicate with policymakers and the public the severity and impact of dental caries and it can add value to the conventional presentation of DMF/dmf data.


Assuntos
Cárie Dentária , Criança , Índice CPO , Humanos , Michigan , Dente Decíduo
12.
J Oral Microbiol ; 12(1): 1729305, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32158514

RESUMO

Background: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 children (6-10 years old) with no caries, early caries, or advanced caries were sequenced for internal transcribed spacer 2 (ITS-2). The mycobiome profiles were correlated with previously published bacteriome counterparts. Interaction among selected fungal and bacterial species was assessed by co-culture or spent media experiments. Results: Fungal load was extremely low. Candida, Malassezia, Cryptococcus, and Trichoderma spp. were the most prevalent/abundant taxa. Advanced caries was associated with significantly higher fungal load and prevalence/abundance of Candida albicans. Cryptococcus neoformans and Candida sake were significantly over-abundant in early caries, while Malassezia globosa was significantly enriched in caries-free subjects. C. albicans correlated with Streptococcus mutans and Scardovia wiggsiae among other caries-associated bacteria, while M. globosa inversely correlated with caries-associated bacteria. In-vitro, M. globosa demonstrated inhibitory properties against S. mutans. Conclusions: the results substantiate the potential role of the oral mycobiome, primarily Candida species, in dental caries. Inter-kingdom correlations and inhibition of S. mutans by M. globosa are worth further investigation.

13.
Curr Dev Nutr ; 4(3): nzaa029, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32215356

RESUMO

BACKGROUND: Chronic stress increases the risk of excess intake of calorie-dense foods. Low-income minority caregivers in the United States are cumulatively exposed to stressors and unhealthy foods, but evidence of this association is limited in this population group. The objective of the current study was to assess the association between chronic stress and unhealthy dietary behaviors among low-income African-American caregivers in Detroit, Michigan. METHODS: Data came from Detroit Dental Health Project, a longitudinal study of pairs of African-American caregivers and children during 2002-2007. A sample of 912 female caregivers were included and their baseline (2002-2003) survey responses were analyzed to identify those with chronic stress and patterns of dietary behaviors. The likelihood of having unhealthy dietary behaviors was compared between chronically stressed caregivers and others, and the mediator role of depressive symptoms or current smoking was tested. RESULTS: Approximately 10% of caregivers experienced chronic stress as they all reported discrimination, residential movement, and lack of social support. Twenty-five percent of the caregivers were found to have an unhealthy dietary pattern characterized by excess intake of high fatty foods and soda. Chronically stressed caregivers were more likely to exhibit unhealthy dietary behaviors (prevalence ratio: 1.39; 95% CI: 1.05, 1.84), and this relation was significantly mediated by depressive symptoms, not current smoking. CONCLUSIONS: These findings suggest that chronic stress played a role in negatively influencing dietary behaviors. As this association might be mediated by depressive symptoms, an intervention to reduce depressive symptoms can be considered as an effective strategy to promote healthy dietary behaviors among chronically stressed minority caregivers.

14.
Caries Res ; 53(5): 532-540, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30889593

RESUMO

An effect of soda intake on dental caries in young children (birth to 5 years) may vary over time. Estimating a dynamic effect may be challenging due to time-varying confounding and loss to follow-up. The purpose of this paper is to demonstrate utility of targeted maximum likelihood estimation (TMLE) method in addressing longitudinal data analysis challenges and estimating a dynamic effect of soda intake on pediatric caries. Data came from the Detroit Dental Health Project, a 4-year cohort study of low-income -African-American children and caregivers. The sample included 995 child-caregiver pairs who participated in 2002-03 (W1) and were followed up in 2004-05 (W2) and 2007 (W3). The outcome was counts of caries surfaces at W3, and the exposure was child's soda intake at W1 and W2. Time-varying covariates included caregiver's smoking status, oral health fatalism, and social support. Forty-three percent of children consistently consumed soda at W1 and W2, whereas 21% were nonconsumers throughout 2 surveys. The remaining 35% switched intake status between W1 and W2. Association between soda intake patterns and caries was tested using TMLE. Children with a consistent soda intake had 1.03 more caries lesions at W3 than those with consistently no soda intake (95% CI 0.09-1.97) on average. If soda was consumed only at W1 or W2, an estimated effect of soda on caries development at W3 was no longer statistically significant. In conclusion, consistent soda intake during the early childhood led to one additional caries tooth surface. The study highlights utility of TMLE in pediatric caries research as it can handle modeling challenges associated with longitudinal data.


Assuntos
Bebidas Gaseificadas/efeitos adversos , Cárie Dentária/epidemiologia , Negro ou Afro-Americano , Pré-Escolar , Feminino , Humanos , Lactente , Funções Verossimilhança , Estudos Longitudinais , Masculino , Michigan , Pobreza , Fatores de Risco
15.
Spec Care Dentist ; 39(2): 201-207, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30761581

RESUMO

BACKGROUND: Health education has a focus on knowledge dissemination and advice giving, which seldom translates into behavioral changes. Motivational interviewing (MI) has potential for helping elderly patients. However, most oral health studies conducted to date have excluded older individuals, and the outcomes examined have been exclusively clinical. This study examines the treatment fidelity of an individual-based MI intervention and assesses baseline characteristics related to older adults' self-efficacy (SE), oral-health-related quality of life (OHRQoL), oral health knowledge, and facilitators, barriers, and confidence on achieving MI goals. METHODS: A convenience sample of 60 seniors was recruited. Participants were obtained through the flow of patients at dental clinics, and received a 45-min individual-based MI education session during a scheduled dental appointment. RESULTS: Regarding MI treatment fidelity, basic proficiency or higher was achieved in all global areas. Significant correlations were found between SE and OHRQoL scores and rating elements of the MI intervention (P = 0.0001). Lack of knowledge related to connections between poly pharmacy and caries risk, and the importance of use of fluorides were evident. CONCLUSION: Findings from the study support the reliability of this new MI intervention. Dental professionals are encouraged to adopt the spirit of MI in their one-on-one patient counseling.


Assuntos
Educação em Saúde Bucal , Entrevista Motivacional , Idoso , Aconselhamento , Cárie Dentária/prevenção & controle , Feminino , Fluoretos Tópicos/administração & dosagem , Humanos , Masculino , Pessoa de Meia-Idade , Philadelphia , Polimedicação , Qualidade de Vida
16.
J Oral Microbiol ; 11(1): 1557986, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30671194

RESUMO

Studies of the microbiome associated with dental caries have largely relied on 16S rRNA sequence analysis, which is associated with PCR biases, low taxonomic resolution, and inability to accurately study functions. Here, we employed whole metagenome shotgun sequencing, coupled with high-resolution analysis algorithm, to analyze supragingival microbiomes from 30 children with or without dental caries. A total of 726 bacterial strains belonging to 406 species, in addition to 34 bacteriophages were identified. A core bacteriome was identified at the species and strain levels. Species of Prevotella, Veillonella, as yet unnamed Actinomyces, and Atopobium showed strongest association with caries; Streptococcus sp. AS14 and Leptotrichia sp. Oral taxon 225, among others, were overabundant in caries-free. For several species, the association was strain-specific. Furthermore, for some species, e.g. Streptococcus mitis and Streptococcus sanguinis, sister strains showed differential associations. Noteworthy, associations were also identified for phages: Streptococcus phage M102 with caries and Haemophilus phage HP1 with caries-free. Functionally, potentially relevant features were identified including urate, vitamin K2, and polyamine biosynthesis in association with caries; and three deiminases and lactate dehydrogenase with health. The results demonstrate new associations between the microbiome and dental caries at the strain and functional levels that need further investigation.

18.
J Evid Based Dent Pract ; 17(2): 119-121, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28501058

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Early Caries Predicts Low Oral Health-Related Quality of Life at a Later Age. Kragt L, van der Tas JT, Moll HA, Elfrink ME, Jaddoe VW, Wolvius EB, Ongkosuwito EM. Caries Res. 2016; 50(5):471-9. SOURCE OF FUNDING: This study was funded by (1) the Erasmus Medical Center, Rotterdam, the Erasmus University, Rotterdam, (2) the Netherlands Organization for Health Research and Development, (3) an unrestricted grant from GABA, Therwil, Switzerland, (4) the Netherlands Organization for Health Research and Development (VIDI 016.136.361), and a (5) consolidator grant from the European Research Council (ERC-2014- CoG-64916). TYPE OF STUDY/DESIGN: Prospective Cohort Study.


Assuntos
Saúde Bucal , Qualidade de Vida , Criança , Humanos , Estudos Prospectivos
19.
J Dent Educ ; 81(5): 500-516, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28461627

RESUMO

Many dental schools have integrated tobacco cessation into their predoctoral curricula. However, dental students' perceptions should be taken into consideration when designing those curricula. The aim of this study was to systematically review the published literature on dental students' attitudes and perceptions regarding tobacco cessation. The research team conducted a search for articles through April 2016 using the following electronic databases: Medline, PsychInfo, Cochrane Library, and CINAHL. Each abstract and/or article was reviewed for inclusion. Data were extracted from all included articles. Each was rated for quality appraisal by two reviewers. The initial search identified 2,035 articles, and 38 of those were included in the review. The majority were cross-sectional and sampled students from one dental school. There was wide variation in the measurement of dental student attitudes. Overall, the majority of students in these studies reported believing it is within the scope of dental practice to address tobacco use with patients, but there was variability in terms of the practice of specific tobacco cessation strategies. The most common perceived barrier was patient resistance/lack of motivation. In most of the studies, the majority of students were interested in being trained in tobacco cessation. The findings suggest that dental students will respond positively to receiving tobacco cessation education while in dental school and that educators should include strategies to help future dentists deal with patient resistance. Future studies should focus on the development of a validated measure of dental student attitudes toward tobacco cessation and longitudinal, multi-institutional research that can provide more generalizable findings.


Assuntos
Atitude Frente a Saúde , Estudantes de Odontologia/psicologia , Abandono do Uso de Tabaco , Currículo , Humanos , Inquéritos e Questionários
20.
J Dent Educ ; 81(3): 300-309, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28250036

RESUMO

The aims of this study were to identify noncognitive factors that dental faculty members perceived to contribute to dental students' success and to assess dental faculty members' ratings of the relative importance of these factors to academic performance, clinical performance, and overall success. Out of 184 eligible faculty members at one U.S. dental school, 43 respondents (23.3%) completed a survey in 2015-16. The survey asked respondents to rank the importance of seven noncognitive factors to academic performance, clinical performance, and overall success. Descriptive analysis was conducted to determine the ratings on importance of each noncognitive factor. Two additional open-ended questions asked faculty members to 1) think of dental students who performed very well and list the noncognitive factors they believed contributed to those students' success and 2) identify the two most important of those factors that contributed to success. Qualitative analysis was conducted to identify themes in the open-ended responses. The respondents rated professionalism and preparedness highest in importance for overall success. Preparedness was rated highest in importance for academic performance, and communication was highest in importance for clinical performance. Six themes were identified in the open-ended responses: communication/interpersonal skills, approach to learning, personal characteristics, professionalism, diverse experiences, and technical abilities. On both open-ended items, the most frequently cited noncognitive skill was communication/interpersonal skills followed by approach to learning. In this study, dental faculty members perceived communication, preparedness, and professionalism as important skills contributing to dental students' success.


Assuntos
Logro , Atitude do Pessoal de Saúde , Docentes de Odontologia/psicologia , Estudantes de Odontologia/psicologia , Atitude , Comunicação , Humanos , Relações Interpessoais , Aprendizagem , Pennsylvania , Profissionalismo
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