RESUMO
The aim of the present study was to study the effect of chronic treatment (9 weeks) of fluoxetine (20 mg/kg p.o.) a selective serotonin reuptake inhibitor on blood glucose level and in prevention of diabetic neuropathic pain perception. Evaluation of diabetic neuropathy was performed after 9 weeks of single injection of streptozotocin (70 mg/kg i.v.) in rats. Blood glucose level, glycated haemoglobin, grip strength, pain sensitivity and threshold in diabetic rats were measured at the end of 9 weeks. The results of the present study indicate that the 9 weeks treatment of fluoxetine demonstrates hypoglycemic effect; it marked decreases the blood glucose level in diabetic treated animals. There was also decrease in the grip strength in diabetic rat indicates to induction of neuropathy or nerve damage. Fluoxetine increase the grip strength of diabetic rats. There was also found loss of pain perception in diabetes rats which measured using hot plate and tail flick methods. Fluoxetine increases the licking time and withdrawal latency in hot plate and tail flick test respectively indicates the presence of pain perception and prevention of nerve damage demonstrates its protective effect in diabetic neuropathy. Our study concludes the chronic treatment of fluoxetine significantly decreases the glycemic level as well as it protected from the development of diabetic neuropathy.
RESUMO
The aim of the present study was to evaluate the effect of ethanolic extract of Murraya koenigii leaves (MKL) on blood glucose level and in prevention or management of diabetic neuropathy. In the present study the diabetic neuropathy was developed 9 weeks after single injection of streptozotocin (STZ, 70 mg/kg i.v.) in rat. The treatment with MKL (300 and 500 mg/kg p.o.) was started after stabilization of blood glucose level (13 days after STZ) and evaluated for determination of glycemic level, glycated haemoglobin, grip strength, pain sensitivity and threshold. The result showed that the treatment with MKL possessed hypoglycemic effect in diabetic treated animals. The results also indicated that the decreases in the grip strength in diabetic animals represented the induction of neuropathy 9 weeks after STZ treatment. Prior treatments with MKL increased the grip strength of diabetic rats. The results of pain sensitivity indicated the loss of pain perception in diabetic animals because of nerve damage. While prior treatment with MKL upto 9 week in diabetic animals resulted in the increase in the licking time and withdrawal latency in hot plate and tail flick tests, respectively, which indicates the presence of pain perception and prevention of nerve damage due to protective effect of MKL in progression of diabetic neuropathic pain. Therefore, the present study concludes that the chronic treatment with MKL significantly decreased the glycemic level as well as it protected the animals against development of diabetic neuropathy.