[Activity of aminopeptidase and inter-alpha-trypsin inhibitor in serum of women in physiological labor]. / Aktywnosc aminopeptydazowa i stezenie inter-alfa-inhibitora trypsyny w surowicy krwi kobiet w okresach porodu fizjologicznego.

Aminopeptidase activity was measured towards L-alanine and L-leucine naphtylamides in the blood serum obtained from the non-pregnant women and from women during the stages of the physiological delivery. In the same samples the concentration of inter-alpha-trypsin inhibitor (alpha ITI) was measured. The highest aminopeptidase activity was observed in the stage II, and it was statistically significant comparing to the stage I, whereas the statistically significant increase in the alpha ITI concentration was noticed in stage I in primiparae and in stage I in multiparae.

Argyrophilic proteins of nucleolar organizer regions (AgNORs) in fallopian tube carcinomas.

Forty-four patients diagnosed with primary cancer of the fallopian tube (PFTC) were analyzed with regard to AgNORs expression, morphological classification, clinical stage and survival rate. Twenty-seven patients were FIGO stage I, 7 FIGO II and 10 FIGO III. Histological classification of PFTC revealed 18 endometrioid types, 9 serous, 7 undifferentiated, 6 urothelial, 2 clear cell and 2 another type of (intestinal, squamous cell) cancer. Histological grading revealed 11 G1, 16 G2 and 17 G3 tumors. The number of AgNORs per nucleus ranged from 1 to 7, mean 2.54+/-0.77. The smallest number of silver stained NORs was observed in the endometrioid type (mean 2.32+/-0.62) and the biggest number of AgNORs in undifferentiated carcinoma (mean 3.05+/-0.82). There was no correlation between number of AgNORs and AgNOR area/nuclear area ratio and survival (p=0.71), histological stage or histological type of PFTC. There was a correlation between the number of AgNORs among tumors with histological grade 1 and grade 3 (p=0.023), and grade 2 and grade 3 (p=0.045). However, there was no correlation between AgNOR number and survival rate in these groups.

[Urinary trypsin inhibitor in pregnant women with normal and pathological course of pregnancy]. / Inhibitor trypsyny w moczu kobiet w ciazy fizjologicznej i patologicznej.

Urinary trypin inhibitor concentration (UTI) was measured by Fritz and all method in the groups of women: non-pregnant (I), pregnant (II), in pregnancy complicated by EPH-gestosis (III) and in the prolongated pregnancy (IV). Furthermore, in the urine from the investigated group the index protein/creatinine was established. There was noticed the statistically significant increase in the UTI concentration in II, III, IV groups comparing to the non-pregnant group--I, and the increase in UTI in III and IV group in comparison to group II. The UTI measurement in the pregnant women connected with the index protein/creatinine could be significant for diagnosing of the pathology of pregnancy.

[Concentration of cysteine proteinase inhibitors in urine, amniotic fluid and serum from women in pregnancy complicated by EPH-gestosis]. / Stezenie inhibitorów proteinaz cysteinowych w moczu, plynie owodniowym i surowicy kobiet w ciazy powiklanej EPH-gestoza.

Cysteine proteinase inhibitors (IPC) concentration was measured by the modified Barrett method using papaine in urine, amniotic fluid and serum obtained from the healthy labored women and from labored women in pregnancy complicated by EPH-gestosis. It was noticed the statistically significant increase in the IPC concentration in the material from the pregnant women with EPH-gestosis comparing to the women, which pregnancy had the physiologically normal course.

[Changes in arylsulphatase activity (EC 3.1.6.1) in the blood serum and urine in women during pregnancy and in the course of delivery]. / Zmiany aktywnosci arylosulfatazy A (EC 3.1.6.1) w surowicy i moczu kobiet w przebiegu ciazy i porodu fizjologicznego.

By the determination of arylsulphatase A activity (EC 3.1.6.1) in the blood serum and urine obtained from 66 women using the modified method by Lee-Vaupel and Conzelmann it was noticed the increase in the enzyme activity during the pregnancy comparing to the non-pregnant group. The highest enzyme activity was observed in the III trimester of pregnancy. In the following stages of delivery (I, II, III) it was assumed the increase in enzyme activity in urine. The highest enzyme activity in urine was observed in the stage III, and in the serum--in the stage II. It was compared the enzyme activity in primiparae and multiparae proving, that in the serum nd urine this activity is higher in the stages I and II in multiparae, and in the stage III in primiparae.

[Changes in arylsulphatase activity (EC 3.6.1) in the blood serum and urine in women during the pregnancy and in the course of delivery]. / Zmiany aktywnosci arylosulfatazy A (EC 3.1.6.1) w surowicy i moczu kobiet w przebiegu ciazy i porodu fizjologicznego.

By the determination of arylsulphatase A activity (EC 3.1.6.1) in the blood serum and urine obtained from 66 women using the modified method by Lee-Vaupel and Conzelmann it was noticed the increase in the enzyme activity during the pregnancy comparing to the non-pregnant group. The highest enzyme activity was observed in the III trimester of pregnancy. In the following stages of delivery (I, II, III) it was assumed the increase in enzyme activity in urine. The highest enzyme activity in urine was observed in the stage III, and in the serum--in the stage II. It was compared the enzyme activity in primiparae and multiparae proving, that in the serum nd urine this activity is higher in the stages I and II in multiparae, and in the stage III in primiparae.

[The arylsulphatase A (EC 3.1.6.1) activity in serum, urine and amniotic fluid from pregnant women with EPH-gestosis]. / Aktywnosc arylosulfatazy A (EC 3.1.6.1) w surowicy, moczu i plynie owodniowym kobiet ciezarnych z EPH-gestoza.

In serum, urine and amniotic fluid obtained from the 52. women divided into three groups the arylsulphatase A (EC 3.1.6.1) activity was measured by the modified Lee-Vaupel and Conzelmann method. It was noticed in serum from the pregnant women with EPH-gestosis the statistically significant (p < 0.05) increase in the enzyme activity comparing to the results from non-pregnant women and pregnant with normal course of pregnancy. It was no statistically significant differences in the urine from pregnant with EPH-gestosis and from the healthy pregnant, but there was the increase (p < 0.01) in the enzyme activity in amniotic fluid from pregnant women with EPH-gestosis comparing to the physiological course of pregnancy. According to our data, the arylsulphatase A activity assay could be recommended as a diagnostic marker in the EPH-gestosis.

[Gamma-glutamyltransferase (EC 2.3.2.2) activity in the course of brachytherapy of the uterine cervix and corpus carcinoma]. / Aktywnosc gamma-glutamylotransferazy (EC 2.3.2.2) w monitorowaniu brachyterapii raka szyjki i trzonu macicy.

The gamma-glutamyltransferase (EC 2.3.2.2) activity was measured in the blood serum obtained from 25 female patients with the uterine cervix carcinoma 10 and from 21 women with the uterine corpus carcinoma 10, treated by brachytherapy. The enzyme activity was determined in the five time points: before the brachytherapy, in the middle of the treating course, immediately after the application of the full dose of radium and in the seventh and the fourteenth day after the finishing the treatment. It was assumed the changes in the enzyme activity during the treatment course, and the highest values were observed immediately after the brachytherapy. It was noticed, that there is the direct connection between the enzyme activity changes and used brachytherapy method.

The concentration of cotinine in urine, colostrum and amniotic fluids within the system mother-baby.

The findings obtained by the authors of the thesis submit the new cognitive values to the diagnosis of pathology of pregnancy i.e. the influence of nicotine on the organisms of a mother and a new-born child, estimated by the assay of cotinine, the most important metabolite of nicotine. The authors lay a particular stress on the "colostrum-milk way" in the mother-child relationship and this is that needs to be emphasized in this thesis.

[Creatine kinase activity ec 2.7.3.2 in blood serum of women during pregnancy and during delivery]. / Aktywnosc kinazy kreatynowej ec 2.7.3.2 w surowicy krwi kobiet w przebiegu ciazy i okresach porodu.

In the serum of women during the pregnancy and during the stages of the delivery the activity of creatine kinase (CK) was measured using the test by Fermognost. The investigations were led in three 20-personal groups of women: non-pregnant, pregnant examined three times, in each trimester of pregnancy, and labored women examined three times, one time in each stage of delivery. The age of investigated women was between 18-39 years. According to obtained results, it was noticed statistically significant increase in the activity of this enzyme during the pregnancy. Measuring the activity of creatine kinase in three stages of delivery, the highest increase in this enzyme activity was observed in the third stage.

Average quantitative concentration of cotinine within the system pregnant woman-baby.

The findings presented by the authors of this report contribute to the diagnosis of pathology of pregnancy i.e. they assess the influence of nicotine on the organisms of a mother and a new-born child, estimated by concentration of cotinine, the most important metabolite of nicotine. The mean proportional share of cotinine in the fluids and organs in the pregnant women smoking actively was as follows: urine 72.1%, amniotic fluids 14.3%, colostrum 8.9% and placenta 4.7%. The authors pay a particular attention to the "colostrum-milk way" in the mother-child relationship what is emphasized in this report.

[Results of tests with clomiphene and choriogonadotropin in men with oligozoospermia treated with longterm antiestrogens]. / Wyniki testu z klomifenem i choriogonadotropina u mezczyzn z oligozoospermia przewlekle leczonych przeciwestrogenowo.

The evaluation was made of the response to testosterone and oestradiol in the test (50 mg twice daily for 10 days) and HCG test (6000 units of Biogonadyl through 2 days) performed on 21 normospermic (fertile) males and on patients with normogonadotropic, idiopathic oligozoospermia, positively (12 patients) or negatively (24 males) reacting to the longterm Clostilbegyt treatment (50 mg daily for 240 days). It was shown that patients with oligozoospermia who do not react with an increase of spermatozoa during the longterm antioestrogen treatment had higher response of oestradiol in the dynamic test (+ 118.9%) than the normospermic males (+ 60.8%) or the oligozoospermic who showed positive semenologic response to the treatment (+ 58.8%). The similar reaction of oestradiol was shown in the HCG stimulation. In the hiperoestrogenism phenomenon the authors noticed the cause of lack of positive semenologic effects of the longterm antioestrogen treatment.

[The problem of marital infertility caused by the male partner pathology in view of potential semen donors]. / Problem bezplodnosci malzenskiej uwarunkowanej patologia partnera meskiego w pogladach potencjalnych dawców nasienia.

An inquiry was carried out in 91 students of the V year of Medical Academy, potential semen donors for artificial insemination, for gaining an insight into the degree of acceptance of this problem in their present situation and in assumed possible infertility of them or their partners, and the foreseen results of sociocultural consequences of the infertility of one of the partners in a married couple, as regarded by the responders. The highest grade of acceptance of semen donation for heterologous insemination was shown by students living in cities and coming from the families of the intelligentsia. Among the factors determining this attitude the most frequent were: altruistic attitude, followed by economic gains. Religion had only a slight influence on this attitude. In the studied group a higher acceptance was gained in case of female infertility by resolving the problem by use of the so called proxy-mother, than by artificial insemination in case of male infertility. Nearly half the responders supported adoption of a child in preference to artificial insemination, while heterologous insemination was preferred mainly by responders coming from small towns and villages.

[Results of heterologous artificial insemination, ethical- deontological implications]. / Wyniki sztucznej inseminacji heterologicznej, implikacje etyczno-deontologiczne.

Fourteen female patients married to infertile men were artificially inseminated with the semen of unknown donors within 1985-1987. Over 150 inseminations (no complications except sporadic pain) produced 7 pregnancies out of which 5 were finished with the delivery of healthy babies, one was terminated in the seventh week with abortion. Fate of the remaining pregnancy is unknown. Ethical-legal implications of AID, the problem of donors and classification of semen, and necessity to store the semen at the temperature of liquid nitrogen due to the risk of AID are emphasized.