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1.
Pak J Biol Sci ; 26(10): 504-509, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38110555

RESUMO

<b>Background and Objective:</b> This plant is known in many parts of the world and is used as medicine in some countries. In Vietnam, people know how to use <i>Passiflora foetida </i>L.,<i> </i>as an herb. There are many research works in the world, most of them focus on medicinal properties. The objective of this study was to determine the genetic diversity of samples of <i>Passiflora foetida </i>L., yellow fruit based on agronomic traits and the gene region of "<i>rbcL</i>". <b>Materials and Methods:</b> Seed samples were arranged in a completely randomized design with three replications. The trial was a sample of <i>Passiflora foetida </i>L., species. The distance between sample plant is 3×2 m, so the total number of trees is 1,330 trees ha<sup>1</sup>. The genetic relationship between them was determined through the construction of a phylogenetic tree in the "<i>rbcL</i>" gene region sequence. Data analysis and processing methods done by using X software. <b>Results:</b> The PCR results that amplify the "<i>rbcL</i>" gene region around 670 bp, the PCR products were then sequenced. The sequence results were compared with the sequences on the NCBI gene bank, showing that the sequences of the nine varieties/species all coincided with the <i>Passiflora foetida </i>Sims species sequences with a high similarity coefficient from 98.77 to 99.9%. Genetically shows that all nine species samples belong to <i>Passiflora foetida</i> which can be classified into three genetic samples of An Giang (LA1), Ca Mau (LA2) and Can Tho (LA3) which are genetically close to each other, have the same index. <b>Conclusion:</b> The heritability in the broad sense is high in the two traits of stem height and leaf length, so it can be considered that these two traits are controlled by genes. The cultivation of algae seeds to improve the productivity of these medicinal plant varieties is important in providing raw materials for the oriental medicine industry.


Assuntos
Passiflora , Humanos , Passiflora/genética , Neurastenia , Filogenia , Agricultura , Preparações Farmacêuticas
2.
Pak J Biol Sci ; 26(11): 586-592, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38193373

RESUMO

<b>Background and Objective:</b> <i>Orthosiphon</i> sp., contains many active ingredients that are beneficial to health. In addition to prevention, they are also used in treatment. This study aims to evaluate the agronomic characteristics of 9 <i>Orthosiphon</i> sp., based on morphology and combine molecular biology methods to determine the genetic relationship based on the "ITS1-4" gene region. <b>Materials and Methods:</b> Nine seed samples/species <i>Orthosiphon</i> sp., grown in 9 provinces in South Vietnam were collected and planted for survey at the Can Tho experimental farm. The method of observing and describing the external morphology and microdissection of the eye cage is carried out. The DNA extraction was performed at the Molecular Biology Laboratory, Institute of Food and Biotechnology, Can Tho University. <b>Results:</b> There are differences in some parameters except leaf width and flower diameter. A simple pedigree chart can classify nine cat breeds into 3 groups: Group I includes An Giang (Râ1), Ca Mau (Râ2) and Can Tho (Râ3) breeds that are genetically close to each other and have only the same quantity, group II includes 2 varieties of Vinh Long (Râ8) and Dong Thap (Râ6), group III includes 4 identical samples in Kien Giang (Râ7), Hau Giang (Râ4), Soc Trang (Râ5) and Tien Giang (Râ9). <b>Conclusion:</b> With molecular biology techniques, 9 cat breeds have been identified, all belonging to the species <i>Orthosiphon aristatus</i>. However, to have more accurate and complete conclusions, it is necessary to study some specific gene sequences related to the morphology and substances contained in the plant.


Assuntos
Orthosiphon , Plantas Medicinais , Humanos , Melhoramento Vegetal , Agricultura , Biotecnologia
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