Bimodal Fucoidan-Coated Zinc Oxide/Iron Oxide-Based Nanoparticles for the Imaging of Atherothrombosis.

A polyol method was used to obtain ultrasmall ZnO nanoparticles (NPs) doped with iron ions and coated with a low molecular weight fucoidan in order to perform in vivo MR and ex vivo fluorescence imaging of athrothrombosis. During the synthesis, the early elimination of water by azeotropic distillation with toluene allowed us to produce NPs which size, determined by XRD and TEM, decreased from 7 nm to 4 nm with the increase of iron/zinc ratios from 0.05 to 0.50 respectively. For the highest iron content (NP-0.50) NPs were evidenced as a mixture of nanocrystals made of wurtzite and cubic phase with a molar ratio of 2.57:1, although it was not possible to distinguish one from the other by TEM. NP-0.50 were superparamagnetic and exhibited a large emission spectrum at 470 nm when excited at 370 nm. After surface functionalization of NP-0.50 with fucoidan (fuco-0.50), the hydrodynamic size in the physiological medium was 162.0 ± 0.4 nm, with a corresponding negative zeta potential of -48.7 ± 0.4 mV, respectively. The coating was evidenced by FT-IR spectra and thermogravimetric analysis. Aqueous suspensions of fuco-0.50 revealed high transverse proton relaxivities (T2) with an r2 value of 173.5 mM-1 s-1 (300 K, 7.0 T) and remained stable for more than 3 months in water or in phosphate buffer saline without evolution of the hydrodynamic size and size distribution. No cytotoxic effect was observed on human endothelial cells up to 48 h with these NPs at a dose of 0.1 mg/mL. After injection into a rat model of atherothrombosis, MR imaging allowed the localization of diseased areas and the subsequent fluorescence imaging of thrombus on tissue slices.

A New Insight into Biospeckle Activity in Apple Tissues.

The monitoring and characterization of agricultural products before harvest or during ripening, storage, and shelf life has recently been increasingly explored in the literature. The analysis of biospeckle activity has potential for the determination of the optimal harvest window, the monitoring of the fruit ripening process, and the detection of diseases and bruising. In this technique, the specimen is illuminated with coherent light and speckle intensity fluctuations are analyzed using diverse methodologies. Prior work shows that biospeckle activity is strongly correlated to physiological indexes conventionally used to evaluate fruit texture and composition. Here, we scrupulously investigate the biospeckle activity of Gala apple fruits during postharvest stages. We simulate realistic conditions for shelf-life monitoring, namely an unknown history of the fruit and storage in an uncontrolled atmosphere. Scattering spot images are acquired with multiple exposure times using a simple optical setup. The contrast, reflecting biospeckle activity, is computed after eliminating inhomogeneous zones. The results show, for the first time, speckle activity at short time scales. The retrieved correlations between speckle parameters and the ratio of apples' firmness to their soluble solids content reveal significant links despite the unknown fruit's origin, harvest date, and storage history.

Gadolinium/terbium hybrid macromolecular complexes for bimodal imaging of atherothrombosis.

We developed a fluorescence imaging microscope system intended for the localization within artery slices of a gadolinium-based macromolecular biospecific magnetic resonance (MR) contrast agent used for the visualization of atherothrombosis. As the contrast agent is not initially fluorescent, we substitute some gadolinium ions for terbium ions to make them fluorescent while preserving their chemical characteristics. A long fluorescence emission time constant enables us to have a suitable signal-to-noise ratio, despite a low intensity, using pulsed illumination and time-gated imaging. Images of rat arteries show that the contrast agent is indeed localized on the specific regions of the tissues. We currently have a tool that allows us to understand and optimize the MR contrast agent.

Fourier transform acousto-optic imaging with a custom-designed CMOS smart-pixels array.

We report acousto-optic imaging (AOI) into a scattering medium using a Fourier Transform (FT) analysis to achieve axial resolution. The measurement system was implemented using a CMOS smart-pixels sensor dedicated to the real-time analysis of speckle patterns. This first proof-of-principle of FT-AOI demonstrates some of its potential advantages, with a signal-to-noise ratio comparable to the one obtained without axial resolution, and with an acquisition rate compatible with a use on living biological tissue.

Detection of bladder urothelial carcinoma using in vivo noncontact, ultraviolet excited autofluorescence measurements converted into simple color coded images: a feasibility study.

PURPOSE: A difficulty in nonmuscle invasive bladder cancers is the diagnosis of flat and small lesions during white light cystoscopy. We assessed a prototype that measures ultraviolet laser induced autofluorescence for endoscopic detection of nonmuscle invasive bladder cancer. MATERIALS AND METHODS: We compared spectroscopic results with histological findings in 3 groups, including normal urothelium, papillary tumors and flat lesions. The developed method is based on exciting the fluorescence of molecules naturally present in tissue using ultraviolet laser pulses. The diagnostic signal was converted into the intensity ratio of the emitted light at approximately 360 and 450 nm. This ratio depends on the histopathological state of the tissue. The signal was converted into a simple color coded image, in which green indicates normal tissue and red indicates neoplasm. RESULTS: A total of 14 patients were included in analysis. At 360 and 450 nm excitation wavelengths the overall fluorescence intensity of bladder tumors was clearly decreased compared to that of normal urothelium regardless of tumor stage or grade. At the 308 nm excitation wavelength the shape of the tumor spectra, including carcinoma in situ, was markedly different from that of normal or nonspecific inflammatory mucosa. The correlation between red images and tumor in the specimen was 100%. No absolute intensity determinations were required since a definite diagnosis was established based on the fluorescence intensity ratio at 360 and 450 nm. CONCLUSIONS: This feasibility study confirms the functionality of our clinical prototype for the noncontact imaging detection of nonmuscle invasive bladder cancer via an endoscope using ultraviolet excited autofluorescence measurements.

Time-resolved diffusing wave spectroscopy with a CCD camera.

We show how time-resolved measurements of the diffuse light transmitted through a thick scattering slab can be performed with a standard CCD camera, thanks to an interferometric protocol. Time-resolved correlations measured at a fixed photon transit time are also presented. The high number of pixels of the camera allows us to attain a quite good sensitivity for a reasonably low acquisition time.

Ultimate spatial resolution with Diffuse Optical Tomography.

We evaluate the ultimate transverse spatial resolution that can be expected in Diffuse Optical Tomography, in the configuration of projection imaging. We show how such a performance can be approached using time-resolved measurements and reasonable assumptions, in the context of a linearized diffusion model.

Asymptotic behavior and inverse problem in layered scattering media.

The main challenge of noninvasive optical biopsy is to obtain an accurate value of the optical coefficients of an encapsulated organ (muscle, brain, etc.). The idea developed by us is that some interesting information could be deduced from the long-time behavior of the reflectance function. This asymptotic behavior is analyzed for layered media in the framework of the diffusion approximation. A new method is derived to obtain accurate values for the optical parameters of the deepest layers. This method is designed to work in a specific long-time regime that is still within the scope of standard time-of-flight experiments but far from being included in the mathematically defined asymptotic region. The limits of this method, linked to the cases where the asymptotic behavior is no longer governed by the deepest layer, are then discussed.

Cerebral hemodynamics and brain oxygen changes related to gravity-induced loss of consciousness in rhesus monkeys.

The aim of the present experiment was to study the influence of +Gz acceleration (head-to-foot inertial forces) onset on cerebral oxygenation changes (cerebral oxy- and deoxy-hemoglobin) and cerebral blood volume (CBV) in order to evaluate the role of cerebral hypoxemia and ischemia in the appearance of +Gz-induced loss of consciousness (G-LOC). We used five rhesus monkeys which were equipped with near infrared spectroscopy optodes fixed onto the parietooccipital cranial bone. G-LOC (isoelectric electrocorticogram) was detected with silver balls electrodes in contact with the dura matter. The animals were centrifuged up to +12 Gz with two onset rates (0.1 and 3 G/s). Cerebral deoxy-hemoglobin increased significantly (max: +30 +/- 6% of control, P < 0.01) only during the 0.1 G/s run. At G-LOC, CBV changes were not related to G-onset rate (P = 0.30; mean change: -32 +/- 6% of control). We conclude that cerebral ischemia is the main mechanism in the occurrence of G-LOC.