RESUMO
Comparative analysis of the proliferative activity of inflammatory cells and distribution of collagen types I and III was carried out around the net materials of polypropylene and titanium coating polypropylene using im- munohistochemical method and polarization microscopy. Experimental modeling of implanted mesh material were made in the soft tissues of the lumbar region of rats. On the 7th postoperative day, quantitative analysis of proliferating cells delected using antibodies to the Ki-67 protein showed, a significant decrease (p < 0.001) in the number of proliferating cells around the network elements of the polypropylene (29.1 ± 5.7 %), when com- pared to similar figures of infiltrates in titanium coating polypropylene (33.6 ± 3.1 %). Similar patterns were found on the 30th day of the experiment--15.9 ± 4.3 and 26.9 ± 3.6%--respectively (p < 0.001). Different types of collagen fibers in the granulomas around various types of implanted mesh material were detected on sections stained with Sirius red at polarizing light. On the 7th day after surgery, the ratio of collagen fibers ty- pe I and III in granulomas around the mesh material made of polypropylene was 1.085 ± 0.022 and this rati around materials of titanium coated polypropylene was higher--1.107 ± 0.013 (p = 0.017). On the 30th posto- perative day in the interface area ratio I/III collagen significantly increased and amounted to 1.174 ± 0.036 and 1.246 ± 0.102, respectively (p = 0.045). Assessing the impact of the use of titanium as a coating on the po- lypropylene, it can be argued that it promotes the formation of collagen I type and a more mature connective tis- sue around the mesh of the implants.
Assuntos
Materiais Revestidos Biocompatíveis/farmacologia , Fibroblastos/efeitos dos fármacos , Implantes Experimentais , Polipropilenos/farmacologia , Titânio/farmacologia , Animais , Animais não Endogâmicos , Proliferação de Células/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/química , Colágeno Tipo I/genética , Colágeno Tipo I/metabolismo , Colágeno Tipo III/genética , Colágeno Tipo III/metabolismo , Feminino , Fibroblastos/citologia , Fibroblastos/metabolismo , Expressão Gênica , Granuloma/metabolismo , Granuloma/patologia , Antígeno Ki-67/genética , Antígeno Ki-67/metabolismo , Masculino , Polipropilenos/química , Ratos , Titânio/químicaAssuntos
Hérnia Ventral , Telas Cirúrgicas , Titânio , Parede Abdominal , Abdominoplastia , Herniorrafia , HumanosRESUMO
The method of plastic repair of the duodenal stump with an invaginated cone formed from the antral part of resected stomach was developed experimentally on 10 cadavers. Experimental surgeries on 16 mongrel dogs demonstrated primary healing and fast epithelisation of this duodenal stump. The method was used in 2 patients with giant penetrating ulcers of the posterior wall of the duodenum, promising results were seen.