[THE MODES OF EVALUATION OF TYPE OF DEHYDRATION IN CHILDREN HOSPITALIZED BECAUSE OF ACUTE INTESTINAL INFECTION].

Every year about 800,000 cases of intestinal infections end in lethal outcome due to dehydration. The different types of dehydration acquire differential approach to correction. Everywhere there is no application of routine detection of osmolarity of blood plasma under exicosis in children in view of absence of possibility of instrumental measurement. The search of techniques is needed to make it possible to indirectly detect types of dehydration in children hospitalized because of acute intestinal infection with purpose to apply rationale therapy of water-electrolyte disorders. The sampling of 32 patients with intestinal infections accompanied with signs of exicosis degree I-III was examined. The detection of osmolarity of blood was implemented by instrumental technique using gas analyzer ABL 800 Flex (Radiometer; Denmark) and five estimate techniques according to results of biochemical analysis of blood. The differences in precision of measurement of osmolarity of blood plasma by instrumental and estimate techniques were compared using Bland-Altman graphic technique. It is established that formula: 2x[Na+kp] + [glucosekp] (mmol/l) is the most recise. Its application provided results comparable with values detected by instrumental mode.

[Role of biocompatible adhesives in the increase of rhizobia physiological activity and productivity of soybean-rhizobia symbiosis].

On the basis of natural exopolysaccharide xanthan and exopolyacrylamide the sticky-gene composition has been developed. Addition of that composition to the culture medium provided a 26.3 times higher viability of Bradyrhizobium japonicum UCM B-6035 cells during its storage. Introduction of plant growth regulators biosil or ivin into this composition increased the survival of rhizobia. Application of gel inoculant B. japonicum favored more intensive growth of rhizosphere microorganisms, nutrient's accumulation in the soil and increased productivity of soybean-Rhizobium symbiosis.

Investigation of nanostructured oxides: synthesis, structure and properties.

The process of forming three-component nanocrystalline fibers and powders of zirconia, yttria and alumina is studied depending on the component ratio and heat treatment temperature. It has been found that in the investigated system at 500-600 degrees C a nanocrystalline triple solid solution is formed, which exists up to 1200 degrees C. Beyond the above temperature, the triple solid solution decomposes into individual components. Specific regularities of changes in the crystalline structure and size of nanograins of oxides of triple solid solutions in the ZrO2(Y2O3)-Al2O3 system are established depending on the composition and thermal action. The structure--crystallite size--physical-chemical property relationship is also considered. The proposed synthesis method enables preparing nanocrystalline fibers and powders with a high degree of dispersion and reactive activity, whose use in composite materials and ceramics improves their service properties.

Terahertz pulse induced intervalley scattering in photoexcited GaAs.

Nonlinear transient absorption bleaching of intense few-cycle terahertz (THz) pulses is observed in photoexcited GaAs using opticalpump--THz-probe techniques. A simple model of the electron transport dynamics shows that the observed nonlinear response is due to THz-electric- field-induced intervalley scattering over sub-picosecond time scales as well as an increase in the intravalley scattering rate attributed to carrier heating. Furthermore, the nonlinear nature of the THz pulse transmission at high peak fields leads to a measured terahertz conductivity in the photoexcited GaAs that deviates significantly from the Drude behavior observed at low THz fields, emphasizing the need to explore nonlinear THz pulse interactions with materials in the time domain.

[Soybean lectin as a component of a composite biopreparation involving Bradyrhizobium japonicum 634b].

The effects of the composite biopreparation Bralec (involving the soybean-specific root nodule bacterium Bradyrhizobium japonicum strain 634b and soybean lectin at concentrations of 500, 50, and 5 microg/ml as major components) on the development and functional activity of soybean-rhizobium symbiosis (development phases of one leaf, four true leaves, and budding) was studied. It was demonstrated that pretreatment of seed with this preparation stimulated the development of both the macro- and microsymbionts. The experimental plants displayed an active accumulation of biomass (by 4-42% higher compared with the variant with inoculation), development of root nodules (the number increased by 11-110% and the weight, by 27-157%). and elevated nitrogen-fixing activity (by 45-204%). The soybean yield increased by 8-10% upon treatment with Bralec 500 and Bralec 5 as compared with the traditional seed bacterization with root nodule bacteria.

[The significance of exometabolites in the formation and operation of the soybean-rhizobium symbiosis]. / Rol' ékzometabolitov v protsesse formirovaniia i funktsionirovaniia soevo-rizobial'nogo simbioza.

The effect of various inoculates of the soybean-specific strain of nodule bacteria Bradyrhizobium japonicum 634b (unwashed cells, cells washed from the exopolysaccharide-protein complex, and cells combined with the complex) on the formation and operation of soybean-rhizobium symbiosis. It was shown that addition of the exopolysaccharide-protein complex doubled the ability of the microsymbiont to form nodules, nodule weight, and the nitrogenase activity of the nodules. Bradyrhizobium japonicum 634b cells washed from exometabolites had lower indices of symbiotic activity than their intact counterparts.

[Formation and function of the bean-rhizobium symbiosis in soy plants upon introduction of strains of Azotobacter and Bacillus species]. / Formirovanie i funktsionirovanie bobovo-rizobial'nogo simbioza u rastenii soi pri introduktsii shtammov rodov Azotobacter i Bacillus.

The effects of bacteria belonging to the genera Azotobacter and Bacillus in a mixed culture with Bradyrhizobium japonicum strains on formation and function of the legume-rhizobium symbiosis of soybean plants were studied. The data showed that the bacterial compositions B. japonicum 634b + B. subtilis 5, B. japonicum 634b + A. chroococcum 20, and B. japonicum 10k + A. vinelandii 56 with a cell ratio of 1:0.1 increased the number and weight of root nodules as well as the height and weight of the aboveground plant parts in almost all the cases by 22-105% compared with the control variants. These binary microbial cultures may be used for development of combined bacterial preparations for soybean.

[Use of highly dispersed materials for culturing and isolation of granular Agrobacterium radiobacter preparations ]. / Primenenie vysokodispersnykh materialov v tekhnologii kul'tivirovaniia i polucheniia granulirovannykh preparatov Agrobacterium radiobacter.

The effects of synthetic and natural high-dispersion materials on the growth of Agrobacterium radiobacter were studied. Natural minerals montmorillonite and palygorskite (10 g/l nutrient medium) were more potent than high-dispersion silica and its modified forms in stimulating growth of Agrobacterium radiobacter. The interaction of Agrobacterium radiobacter with clay minerals increased the survival rate of bacteria at supraoptimal temperatures. We elaborated new granular bacterial preparation, which enhanced the productivity of cucumbers by 12-15%.

[Granulated preparations of azotobacter in a clay-mineral base]. / Granulirovannye preparaty azotobaktera na osnove glinstykh mineralov.

Interaction of Azotobacter chroococcum 20 cells with clay minerals increased their viability at supraoptimal temperatures. Therefore, clay minerals were used to develop granular bacterial preparations with high viable cell counts and stable compositions during long-term storage. The titers of viable bacteria in the preparations remained 60-70% of the initial level after 12-month storage.

[Morphological changes of lymphatic system in patients with the lymphedema of lower extremities]. / Morfologicheskie izmeneniia limfaticheskoi sistemy u bol'nykh s limfedemoi nizhnikh konechnostei.

Morphological changes in inguinal lymph nodes, lymphatic collector and subcutaneous space were studied up in patients with the lower extremities lymphoedema. While the lower extremities lymphoedema occurs in inguinal lymph nodes the connective tissues spreading in two variants is observed. In the first one fibroblasts and collagen fibres appears in medullar substance (chordae medullaris and medullar sinuses) and in the second the sclerosis process begins from cortical plateau and paracortical zone. It is expedient to include the intraarterial infusion of the xenospleen preparations in complex of treatment of patients with initial stage of the lower extremities lymphoedema, especially in the absence of possibility to proceed with microsurgical intervention and the erysipelas presence in anamnesis.

[Variants and stages of regional lymph nodes sclerosis in lymphedema]. / Varianty i stadii skleroza regionarnykh limfaticheskikh uzlov pri limfedeme.

The inguinal lymph nodes of patients with primary lymphedema of lower extremities were investigated by light microscopy. It was found that sclerotization of regional lymph nodes can begin with both cortical and medullary parenchyma. Different stages of this process in lymph nodes were distinguished. These stages depend on the duration of the illness and the zone of organ where the first signs of sclerotization were observed. Small islands of lymphoid parenchyma were found in even well advanced sclerosis lymph nodes, and this allows to assume possibility of full regeneration of all structure of these organs when lymph circulation is normalized.