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1.
Med Parazitol (Mosk) ; (3): 44-5, 2011.
Artigo em Russo | MEDLINE | ID: mdl-21932546

RESUMO

The efficacy of a kit of Opisthorchiasis-CIC-EIA-Best reagents was evaluated using 270 sera from patients in the study and control groups. The kit showed a sufficient sensitivity (not less than 87.2%) and a high specificity (not less than 97.9%). The use of the above kit of the reagents for enzyme immunoassay in practical healthcare enables one to increase detection rates among the infested subjects on comprehensive examination of those with suspected opisthorchiasis.


Assuntos
Anticorpos Anti-Helmínticos/imunologia , Complexo Antígeno-Anticorpo , Antígenos de Helmintos/imunologia , Técnicas Imunoenzimáticas , Opistorquíase , Opisthorchis/crescimento & desenvolvimento , Animais , Complexo Antígeno-Anticorpo/imunologia , Estudos de Casos e Controles , Fezes/parasitologia , Humanos , Opistorquíase/sangue , Opistorquíase/diagnóstico , Opistorquíase/imunologia , Opistorquíase/parasitologia , Contagem de Ovos de Parasitas , Federação Russa , Sensibilidade e Especificidade
2.
Khirurgiia (Mosk) ; (1): 25-9, 2010.
Artigo em Russo | MEDLINE | ID: mdl-20336041

RESUMO

Results of the immune-enzyme assay (IEA) of children with liver and lung echinococcosis before and after the operation were analyzed. The IEA accuracy was 91% for liver echinococcosis and 57% for lung echinococcosis. Sensitivity was 86% and 55, respectively. IEA was a reliable method of disease recurrence detection postoperatively. Conservative treatment of hydatid disease consisted of Antiparasitic and auxiliary therapy and was successfully applied in 7 patients with liver echinococcosis and 3 kids with combined affection. Preventive chemotherapy was carried out in 115 patients. During the 5 years follow-up period no recurrences was registered.


Assuntos
Anticestoides/uso terapêutico , Equinococose Hepática/terapia , Equinococose Pulmonar/terapia , Laparoscopia/métodos , Cirurgia Torácica Vídeoassistida/métodos , Adolescente , Animais , Anticorpos Anti-Helmínticos/análise , Criança , Pré-Escolar , Equinococose Hepática/diagnóstico , Equinococose Pulmonar/diagnóstico , Echinococcus/imunologia , Echinococcus/isolamento & purificação , Feminino , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Masculino , Estudos Retrospectivos , Tomografia Computadorizada por Raios X , Resultado do Tratamento
5.
Med Parazitol (Mosk) ; (1): 20-4, 2000.
Artigo em Russo | MEDLINE | ID: mdl-10808712

RESUMO

The major proteins of T. gondii excretory and secretory antigens (ESA) obtained during cultivation of tachyzoites by using cultured Vero cells were shown to have molecular weights of 79, 70, 57, 48, 36, and 29 kD. ESA and somatic antigen immunoblotting demonstrated that there were noticeable differences in the immunoactive proteins of these antigens. The antibodies of the sera of patients with toxoplasmosis mainly interacted with ESA proteins having molecular weights of 79, 70, 57, 48, 36, and 29 kD, 57-kD ESA protein antibodies being present on the immunoblots of all the tested sera. When ESA was used as an antigen during enzyme immunoassay, it showed a high sensitivity in the detection of IgM antigens in congenital toxoplasmosis. At the same time, ESA identified the antibodies of this class in the sera of healthy donors much infrequently than somatic antigen (p < 0.05).


Assuntos
Antígenos de Protozoários/análise , Antígenos de Protozoários/imunologia , Estágios do Ciclo de Vida , Proteínas de Protozoários/análise , Proteínas de Protozoários/imunologia , Toxoplasma/crescimento & desenvolvimento , Toxoplasma/imunologia , Animais , Complexo Antígeno-Anticorpo/sangue , Antígenos de Protozoários/classificação , Estudos de Casos e Controles , Chlorocebus aethiops , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina G/imunologia , Peso Molecular , Proteínas de Protozoários/classificação , Sensibilidade e Especificidade , Testes Sorológicos , Toxoplasmose/imunologia , Toxoplasmose Congênita/imunologia , Células Vero
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