RESUMO
Human papillomavirus (HPV) is a highly contagious virus that is linked to cervical cancer and is a major public health issue. Saudi Arabia national efforts aimed to have females aged 9-25 years to receive the available vaccine. Objectives: This study aims to explore parental knowledge, views, and perceptions around HPV in Saudi Arabia. Methods: A cross-sectional online questionnaire was used through direct interview to collect information from a group of parents attending King Abdul-Aziz University Hospital in Jeddah, Saudi Arabia. The questionnaire comprised items including demographics, knowledge, views, and perceptions of HPV infection and vaccination. Results: Of the 500 parents who participated in this study, only 54 (11%) had heard about HPV being associated with cervical cancer. The majority of the participants reported being from the middle social class (n = 472, 94.4%). The vast majority (n = 483, 96.8%) had never heard of the HPV vaccine and (n = 470, 94%) were unwilling to vaccinate their daughters. The primary reported reason behind refusing the vaccine was the lack of information on the importance of HPV vaccination (n = 426, 85.2%). The majority (n = 419, 83.8%) believed that there was insufficient information regarding vaccine safety and efficacy. Participants suggested ways to increase vaccination acceptance like social media awareness (n = 369, 73.8%), educational seminars in schools (n = 254, 50.8%), and a governmental platform (n = 218, 43.6%) providing information on HPV infection and promoting vaccination. Conclusion: Parents in the present study demonstrated a substantial lack of knowledge about HPV infection and vaccination, which may necessitate the establishment of national awareness campaigns.
RESUMO
PURPOSE: This review presents the current methods used for determining ethyl glucuronide (EtG) and ethyl sulfate (EtS) concentrations in postmortem specimens, including sample preparation, analysis and the role of EtG and EtS in the postmortem assessment of the extent of alcohol abuse. METHODS: Papers pertaining to postmortem investigation were collected from scientific databases and reviewed. The papers were published between January 2006 and October 2020. RESULTS: Most of the analyses involved postmortem blood and urine samples, with a few reports using other bodily specimens and tissues. The method validation was not conducted for all applications. These reports were mostly intended to present data rather than interpret them, and the lack of effort in relating these ethanol biomarkers with the cause of death and/or determination of the time of deaths due to ethanol intoxication might decrease the applicability of these makers after a promising start between 2006 and 2010. Nevertheless, by the beginning of 2020, papers investigating ethanol biomarkers were still increasing. A considerable number of methods used liquid chromatography coupled with mass spectrometry (LC-MS) techniques that require less sample preparation (e.g., protein precipitation extraction, dilution, filtration, and centrifugation). Although solid-phase extraction can be applied, only three applications were reported. CONCLUSIONS: Matrix effects can be a substantial challenge in analytical methods based on LC-MS because they directly affect the ionization of analytes. However, these problems can be avoided due to the high cutoff values used to identify positive results for these ethanol biomarkers, which are often above 0.1-1 mg/L, and using internal standards. Research on using tissue specimens is recommended as most of the reported results on this type of specimen were promising.
