RESUMO
Technetium-99m was standardised by the 4π(LS)ce-γ coincidence extrapolation method. Sensitivity of the 4π(LS) channel to two types of radiation, namely conversion electrons and γ-rays, resulted in incorrect activity values being obtained when this was not adequately accounted for. Measurements were more robust when the LS detection efficiency was optimised, and when a γ-window setting was used that monitored the combined LS efficiency for conversion electrons and γ-rays. The primary standard was internationally compared through participation in the BIPM.RI(II)-K4.Tc99m key comparison.
RESUMO
In response to the increasing application of 68Ge/68Ga and 68Ga in nuclear medicine, an international comparison of activity measurement of 68Ge in equilibrium with 68Ga was organised. ANSTO standardised the comparison solution by the 4π(LS)ß+-γ coincidence extrapolation and TDCR efficiency calculation methods, with excellent agreement between the two results. The primary standard was transferred to the ANSTO Secondary Standard Ionisation Chamber. Internationally traceable Australian Certified Reference Materials (ACRMs) of 68Ge/68Ga can now be prepared in various measurement geometries applied in nuclear medicine.
RESUMO
A case of primigravida in whom anti-C, anti-D, and anti-Kpb developed during the third trimester is described. Her first-born infant, delivered at 40 weeks' gestation, was mildly affected with hemolytic disease of the newborn and responded well to phototherapy. Her second-born infant, delivered at 36 weeks' gestation, had a positive direct antiglobulin test, but required no treatment. Clinical and laboratory aspects of both pregnancies are discussed.
Assuntos
Antígenos de Grupos Sanguíneos , Eritroblastose Fetal/etiologia , Sistema do Grupo Sanguíneo de Kell , Sistema do Grupo Sanguíneo Rh-Hr/imunologia , Adulto , Formação de Anticorpos , Feminino , Transfusão Feto-Materna/imunologia , Humanos , Paridade , GravidezAssuntos
Herpes Genital/complicações , Doenças do Colo do Útero/complicações , Neoplasias do Colo do Útero/etiologia , Animais , Azatioprina/efeitos adversos , Colo do Útero/lesões , Dietilestilbestrol/efeitos adversos , Dietilestilbestrol/análogos & derivados , Feminino , Masculino , Ratos , Ratos Endogâmicos , Reserpina/efeitos adversos , Displasia do Colo do Útero/induzido quimicamente , Displasia do Colo do Útero/etiologia , Neoplasias do Colo do Útero/induzido quimicamenteRESUMO
An experimental model is described whereby human and monkey cervical tissues may be maintained as organ cultures for 21 and 40 days, respectively. Inclusion of sodium carboxymethyl cellulose in the culture medium prolongs the survival time of tissues considerably. The sequential cytologic changes associated with herpesvirus hominis type II (HVH-II) infection are reported. These changes are considered in relation to the possible causal role of HVH-II infection in cervical carcinogenesis.
Assuntos
Herpes Simples/complicações , Modelos Biológicos , Técnicas de Cultura de Órgãos , Lesões Pré-Cancerosas , Neoplasias do Colo do Útero/etiologia , Animais , Carboximetilcelulose Sódica , Meios de Cultura , Epitélio/patologia , Feminino , Haplorrinos , Herpes Simples/patologia , Humanos , Neoplasias do Colo do Útero/patologiaRESUMO
An animal model is described that demonstrates the development of varying degrees of basal cell hyperplasia, metaplasia, and dysplasia following cervicovaginal herpesvirus hominis type II (HVH-II) infection. Although the study spanned a period of 30 months, the equivalent of 60 human years, there was no evidence of anaplasia. These results are considered in relation to the role of HVH-II as an initiator in the multistep process leading to cervical carcinoma.
Assuntos
Herpes Simples/complicações , Lesões Pré-Cancerosas , Neoplasias do Colo do Útero/etiologia , Animais , Colo do Útero/patologia , Modelos Animais de Doenças , Feminino , Herpes Simples/patologia , Hiperplasia , Metaplasia , Ratos , Doenças do Colo do Útero/complicações , Doenças do Colo do Útero/patologia , Displasia do Colo do Útero/etiologia , Neoplasias do Colo do Útero/patologia , Doenças Vaginais/complicações , Doenças Vaginais/patologiaRESUMO
An animal model is presented that demonstrates that following cervicovaginal inoculation, infective doses of herpesvirus hominis type II induce a form of herpetic encephalitis. Immunofluorescent results indicate that there is both neural and hematogenous spread of the herpes infection. The possible role of a latent viral infection in the genesis of an acute disease of the central nervous system is investigated in this experimental system.
Assuntos
Colo do Útero/microbiologia , Encefalite/transmissão , Herpes Simples/transmissão , Vagina/microbiologia , Animais , Córtex Cerebral/patologia , Modelos Animais de Doenças , Encefalite/microbiologia , Encefalite/patologia , Feminino , Imunofluorescência , Herpes Simples/microbiologia , Herpes Simples/patologia , RatosRESUMO
Proof of latency of herpesvirus hominis type II (HVH-II) following acute vaginocervical infection must be validated if it is to be considered an etiologic factor in carcinoma of the cervix. An animal model is presented which demonstrates acute HVH-II vaginocervical infection. Following the primary acute infection, animals maintained for an equivalent of 60 human years display persistance of the herpesvirus in the pelvic sensory ganglia. Evidence of the specificity of HVH-II as the infectious agent is presented.