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1.
Bioresour Technol ; 393: 130053, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37993069

RESUMO

Recent decarbonization efforts have led to interests in producing more bio-based chemicals. One attractive compound produced biochemically is the platform chemical known as 2,3-butanediol (2,3-BDO). In this work a mild alkaline pretreatment using sodium carbonate was performed on corn stover (CS) and switchgrass (SG) to generate hydrolysates for fermentation with the 2,3-BDO producer bacteria strain Paenibacillius polymyxa. Enzymatic hydrolysis performed on the pretreated CS and SG produced theoretical sugar yields of 80 % and 95 % for glucose and xylose, respectively. Fermentations with P. polymxya conducted in anaerobic bottles produced 2,3-BDO reaching concentrations ranging from 14 to 18 g/L with negligible conversion into acetoin. Bioreactor fermentations using the hydrolysate media generated up to 43 g/L and 34 g/L of 2,3-BDO from pretreated CS and SG, respectively, within 24 h of fermentation. However, 2,3-BDO product output was reduced by 40-50 % over the remainder of the fermentation due to conversion into acetoin caused by glucose depletion.


Assuntos
Paenibacillus polymyxa , Fermentação , Acetoína , Butileno Glicóis , Glucose
2.
Appl Biochem Biotechnol ; 182(1): 400-410, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27878743

RESUMO

Winter barley has attracted strong interest as a potential feedstock for fuel ethanol production in regions with mild winter climate such as the mid-Atlantic and northeastern USA. Ten recently developed and improved winter barley cultivars and breeding lines including five hulled and five hull-less lines were experimentally evaluated for potential ethanol production. The five hulled barley lines included three released cultivars (Thoroughbred, Atlantic, and Secretariat) and two breeding lines (VA09B-34 and VA11B-4). The five hull-less lines also included three released cultivars (Eve, Dan, and Amaze 10) and two breeding lines (VA08H-65 and VA13H-34). On the average, the hull-less barley cultivars produced more ethanol per unit mass because of their higher starch and ß-glucan contents. However, since the hulled barley cultivars had higher agronomic yield, the potential ethanol production per acre of land for the two types were approximately equal. Among the ten cultivars tested, the hull-less cultivar Amaze 10 was the best one for ethanol production. The ethanol yield values obtained for this cultivar were 2.61 gal per bushel and 292 gal per acre.


Assuntos
Biocombustíveis , Etanol/metabolismo , Hordeum/química , Amido/química , beta-Glucanas/química , Fermentação , Glicosídeo Hidrolases/química , Hordeum/crescimento & desenvolvimento , Hordeum/metabolismo , Melhoramento Vegetal , Proteínas Recombinantes/química , Estações do Ano , alfa-Amilases/química , beta-Glucosidase/química
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