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1.
Diving Hyperb Med ; 52(1): 35-43, 2022 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-35313371

RESUMO

INTRODUCTION: It is now known that COVID-19 has long term effects that may not correlate with clinical severity of disease. The known pulmonary and cardiovascular changes as well as thrombotic tendency could predispose to diving accidents. We aimed to investigate COVID-19 related changes that may cause disqualification from diving among divers who recovered from the disease. METHODS: Occupational and recreational divers who applied for fitness to dive (FTD) assessment after COVID-19 infection were included. Routine FTD assessments were performed. Details of COVID-19 history were evaluated. Lung computed tomography (CT) scans were advised if not previously performed or if there were COVID-19 related changes in previous scans. Divers with pathological findings were restrained from diving and followed prospectively. RESULTS: Forty-three divers were analysed. Thirteen divers were restrained from diving, all due to persistent COVID-19 related changes in lung CT. The prevalence of CT with at least one lung lesion was 68.2% at the time of diagnosis, 73.3% in the first three months after diagnosis and 19.2% later. The most common CT findings were glass ground opacities and fibrotic changes. Demographic characteristics and COVID-19 history of divers deemed 'unfit' were similar to those deemed 'fit'. CONCLUSIONS: Divers who recover from COVID-19 should undergo FTD assessments before resuming diving. A chest CT performed at least three months after diagnosis may be suggested.


Assuntos
COVID-19 , Mergulho , Acidentes , COVID-19/epidemiologia , Mergulho/efeitos adversos , Exercício Físico , Humanos , Prevalência
2.
Diving Hyperb Med ; 50(3): 244-249, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-32957126

RESUMO

INTRODUCTION: Noise has physical and psychological effects on humans. Recommended exposure limits are exceeded in many hospital settings; however, information about sound levels in hyperbaric oxygen treatment chambers is lacking. This study measured in-chamber sound levels during treatments in Turkish hyperbaric centres. METHODS: Sound levels were measured using a sound level meter (decibel meter). All chambers were multiplace with similar dimensions and shapes. Eight measurements were performed in each of 41 chambers; three during compression, three during decompression, and two at treatment pressure, one during chamber ventilation (flushing) and one without ventilation. At each measurement a sound sample was collected for 25 seconds and A-weighted equivalent (LAeq) and C-weighted peak (LCpeak) levels were obtained. Recorded values were evaluated in relation to sound level limits in regulations. RESULTS: The highest sound level measured in the study was 100.4 dB(A) at treatment pressure while ventilation was underway and the lowest was 40.5 dB(A) at treatment pressure without ventilation. Most centres had sound levels between 70 dB and 85 dB throughout the treatment. Ventilation caused significant augmentation of noise. CONCLUSIONS: The chambers were generally safe in terms of noise exposure. Nevertheless, hyperbaric chambers can be very noisy environments so could pose a risk for noise-related health problems. Therefore, they should be equipped with appropriate noise control systems. Silencers are effective in reducing noise in chambers. Thus far, hyperbaric noise research has focused on chambers used for commercial diving. To our knowledge, this is the first study to investigate noise in hospital-based chambers during medical treatments.


Assuntos
Oxigenoterapia Hiperbárica , Humanos , Ruído , Oxigênio
3.
Undersea Hyperb Med ; 47(3): 471-475, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32931675

RESUMO

When commercial sea harvesters have dive accidents, it is sometimes difficult to obtain an accurate dive history and make a definitive diagnosis. We report a sea harvest diver who dived to collect sea snails (Rapana venosa) by using a hookah dive system. He experienced mediastinal and subcutaneous emphysema due to interruption of breathing airflow. Thoracic computed tomography performed one year prior to the accident revealed paramediastinal subpleural blebs on both lung apices. Emphysema was resolved by administering normobaric oxygen.


Assuntos
Mergulho/efeitos adversos , Enfisema Mediastínico/etiologia , Doenças Profissionais/etiologia , Enfisema Subcutâneo/etiologia , Adulto , Humanos , Masculino , Enfisema Mediastínico/diagnóstico por imagem , Enfisema Mediastínico/terapia , Doenças Profissionais/diagnóstico por imagem , Doenças Profissionais/terapia , Oxigênio/uso terapêutico , Enfisema Subcutâneo/diagnóstico por imagem , Enfisema Subcutâneo/terapia , Tomografia Computadorizada por Raios X
4.
Undersea Hyperb Med ; 47(2): 203-210, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32574436

RESUMO

Simulated flight in a hypobaric chamber is a fundamental component in the physiological training of aviators. Although rare, there is always a risk of decompression sickness (DCS) in trainees during hypobaric hypoxia training. In this study we aimed to determine the incidence of altitude-induced DCS and the symptoms manifested in trainees and inside chamber observers (ICOs) during the training sessions. We retrospectively reviewed the records of DCS cases during the period of January 1, 2011, and October 1, 2018. The records of 6,657 trainees and 615 ICOs were evaluated. The gender distribution in 6,657 trainees was 6,578 (98.81%) male and 79 (1.19%) female. The numbers of DCS cases in trainees and ICOs were six (0.09%) and two (0.33%), respectively [(ICOs versus trainees - odds ratio (OR): 3.574; 95% CI 0.720-17.744; (p > 0.05)]. All ICOs were male; no DCS incident was observed among female trainees. Recompression treatments were applied on site, and complete recovery was achieved in all cases. Overall DCS incidence was found to be 0.11% among the 7,193 male subjects, which included trainees and ICOs. The higher incidence of DCS in ICOs was attributed to the physical activities performed at altitudes by ICOs. In such training, established instructions have to be strictly followed by physicians, ICOs and trainees. All trainees and ICOs should be aware of the symptoms and signs of DCS, and medical support including a recompression facility, should be provided on site during hypobaric hypoxia training.


Assuntos
Doença da Altitude/complicações , Doença da Descompressão/epidemiologia , Hipóxia/complicações , Treinamento por Simulação/métodos , Doença da Descompressão/terapia , Feminino , Humanos , Incidência , Masculino , Militares , Razão de Chances , Estudos Retrospectivos , Distribuição por Sexo , Treinamento por Simulação/estatística & dados numéricos
5.
Undersea Hyperb Med ; 45: 489-494, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30428237

RESUMO

The Eurasian Tunnel is a 5.64-km crossroad tunnel that connects Europe and Asia. Located under the seabed for the first time, 3.34 km of the tunnel that crosses the Bosphorus was built by advanced tunneling techniques. An exclusively designed tunnel boring machine (TBM), which has an operating pressure of 11 bars and a diameter of 13.7 meters was used for boring the seabed tunnel. The deepest point was 106 meters below sea level. One bounce diving period and seven saturation diving periods were needed for the repair and maintenance of the TBM during the project. Total time spent under pressure was 5,763 hours. A saturation decompression chamber for four divers was used for the saturation interventions, and divers breathed trimix at storage and excursion depths. The longest saturation run was the second, with storage at 10 bars and excursions to 10.4 bars. Twenty-three professional divers who were all experienced in compressed-air work were assigned to work on the project. Four dive physicians provided medical support, which included screening of divers before and during the hyperbaric interventions as well as on-site supervision. There were no diving-related accidents. A minor hand trauma, an external otitis and occasional insomnia were non-diving-related health issues that occurred during saturation and bounce diving. To our knowledge, the Eurasian Tunnel was the first project to perform TBM repair operations at such depths under the seabed and the first saturation diving in Turkey. In this report, we aimed to share our experiences of hyperbaric medical consulting in support of this type of tunneling project.


Assuntos
Ar Comprimido , Descompressão/métodos , Mergulho/fisiologia , Arquitetura de Instituições de Saúde/instrumentação , Arquitetura de Instituições de Saúde/métodos , Instalações de Transporte , Adulto , Ásia , Dióxido de Carbono , Mergulho/efeitos adversos , Mergulho/legislação & jurisprudência , Europa (Continente) , Hélio , Humanos , Umidade , Manutenção/métodos , Manutenção/organização & administração , Masculino , Pessoa de Meia-Idade , Nitrogênio , Doenças Profissionais/etiologia , Exposição Ocupacional , Oxigênio , Pressão Parcial , Admissão e Escalonamento de Pessoal/organização & administração , Aptidão Física , Pressão , Distúrbios do Início e da Manutenção do Sono/etiologia , Fatores de Tempo , Turquia
6.
Undersea Hyperb Med ; 44(4): 345-355, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28783891

RESUMO

This study aimed to explore the effects of hyperbaric oxygen (HBO2) on blood-brain barrier (BBB) integrity in rats, when administered for one (at 2.5 ATA, 3 HBO2 sessions a day) and five days (at 2.5 ATA, 3 HBO2 sessions a day for the first two days, and twice a day for the last three days). Horseradish peroxidase (HRP) was used to evaluate the BBB permeability. Superoxide dismutase (SOD) activity, glutathione (GSH) and malondialdehyde (MDA) levels were measured in the cerebral cortex and hippocampus regions. Frequent vesicles containing HRP reaction products were observed in capillary endothelial cells in the cerebral cortex and hippocampus of rats subjected to HBO2. The accumulation of HRP reaction products in these brain regions was significantly higher than that of control animals (P ⟨ 0.01). In animals that received HBO2, MDA levels (P ⟨ 0.01 for five days) and GSH (p ⟨ 0.05 for one day, and P ⟨ 0.01 for five days) were decreased in the cerebral cortex, whereas SOD activities slightly increased in this region. In animals that received HBO2 significant decreases in MDA (P ⟨ 0.05 for one day; P ⟨ 0.01 for five days) and GSH (P ⟨ 0.05 for five days) levels were observed in the hippocampus region, but SOD activities decreased in this region. We showed that HBO2 administered with the doses described above impaired BBB integrity in otherwise healthy rats. Therefore, we suggest that the results of this study should be taken into consideration when patients are exposed to HBO2 with the same doses.


Assuntos
Barreira Hematoencefálica/metabolismo , Córtex Cerebral/química , Glutationa Peroxidase/análise , Hipocampo/química , Oxigenoterapia Hiperbárica/efeitos adversos , Malondialdeído/análise , Superóxido Dismutase/análise , Animais , Capilares/ultraestrutura , Córtex Cerebral/irrigação sanguínea , Feminino , Hipocampo/irrigação sanguínea , Peroxidase do Rábano Silvestre/farmacocinética , Oxigenoterapia Hiperbárica/métodos , Microscopia Eletrônica , Permeabilidade , Ratos , Ratos Wistar , Fatores de Tempo
7.
Clin Hemorheol Microcirc ; 62(1): 79-88, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26410860

RESUMO

Inhalation of 100% oxygen in a hyperbaric chamber has been accepted as a useful treatment for patients with various pathologies who suffer from hypoxia. The oxidative effects of hyperbaric oxygen (HBO) on RBCs have been investigated in animals but there is not enough data on hemorheological parameters in patients following HBO treatment (HBOT).In this study, we investigated the effect of HBO on hemorheological and haematological parameters during treatment. Red blood cell (RBC) deformability and aggregation, blood and plasma viscosity and superoxide dismutase activity were investigated in patients who underwent HBOT. Hematological parameters were determined by an electronic hematology analyzer. A Laser-assisted Optical Rotational Cell Analyzer (LORCA) was used to measure RBC deformability. RBC aggregation was measured for cells in autologous plasma and for cells resuspended in PBS containing Dextran70 (3% ) by using a Myrenne Aggregometer. A Wells-Brookfield cone/plate rotational viscometer was used for viscosity measurements. According to our results, a significant decrement of the hematocrit and the RBC count was observed after the 20th session of HBOT compared to the baseline, but none of the hemorheological parameters changed significantly. Our results showed that HBOT did not cause any significant changes in hemorheological parameters, thereby not representing any problems for the patients.


Assuntos
Hipóxia Celular/imunologia , Hemorreologia/imunologia , Oxigenoterapia Hiperbárica/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
8.
Undersea Hyperb Med ; 43(7): 821-825, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28777519

RESUMO

Surgical excision and lay-open is a well-known technique for the treatment of sacrococcygeal pilonidal disease, which impairs a patient's quality of life considerably since wound healing takes a substantial amount of time. It is known that with this method total healing period is longer, but recurrence rate of the disease is lower. The beneficial effects of hyperbaric oxygen (HBO2) therapy on wound healing have been well established since it was first put into in clinical use. The purpose of this prospective randomized clinical trial was to investigate the effects of HBO2 therapy on wound healing in the patients who had sacrococcygeal pilonidal disease and surgical treatment. Total epithelialization times of 12 patients (Group 1) who received surgical intervention were compared with those of 10 patients who had surgical intervention and HBO2 therapy (Group 2). In both groups excised tissue volume, excised skin area, body mass index, blood hemoglobin, albumin levels, ages and duration of the complaints were recorded and there was no statistically significant difference in these parameters except albumin levels when compared. The complete epithelialization time was significantly shorter in Group 2 (50 ± 11 vs. 83 ± 18, p⟨0.001). We conclude that HBO2 had beneficial effects on wound healing, in the patients who had sacrococcygeal pilonidal disease and were treated with surgical excision applying lay-open technique.


Assuntos
Oxigenoterapia Hiperbárica , Seio Pilonidal/cirurgia , Cicatrização , Fatores Etários , Índice de Massa Corporal , Humanos , Seio Pilonidal/sangue , Estudos Prospectivos , Albumina Sérica/análise , Fatores de Tempo
9.
Undersea Hyperb Med ; 41(3): 217-21, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24984316

RESUMO

The purpose of this study was to review the cases diagnosed as decompression sickness (DCS) with recompression therapy treament between 1963 and 1998 in Turkey. The records of 179 cases were analyzed for age, gender, type of DCS, presenting signs and symptoms, time to onset of symptoms, time to recompression therapy, recompression table used, total number of treatments and outcomes. The diving depth on the day of injury ranged between 60 and 215 fsw (18 and 65 msw). The symptoms developed during ascent or within 10 minutes after surfacing in 47% of divers and within the first hour in 87% of the divers. The most frequent symptom was sensory loss in the extremities. The dive before the symptoms was a repetitive dive in 50% of the cases and the diagnosis was Type II DCS in 79% of these divers. Most of the divers (84%) received recompression therapy after a delay of more than 12 hours. Healing rate was 68% with the air recompression tables and 86% with the oxygen tables. Repetitive dives were associated with a higher incidence of Type II DCS than single dives (79% and 66%, respectively). The results using recompression tables with oxygen were more successful than the treatment with air tables.


Assuntos
Doença da Descompressão/terapia , Mergulho/efeitos adversos , Oxigenoterapia Hiperbárica/métodos , Adolescente , Adulto , Doença da Descompressão/etiologia , Humanos , Oxigenoterapia Hiperbárica/normas , Masculino , Pessoa de Meia-Idade , Valores de Referência , Estudos Retrospectivos , Fatores de Tempo , Turquia , Adulto Jovem
10.
Eur Arch Otorhinolaryngol ; 271(8): 2213-7, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24362587

RESUMO

We aimed to evaluate nasal mucosal changes and efficiency of nasal steroids and diclofenac on nasal mucosa during hyperbaric oxygen (HBO) treatment. Forty adult Albino-Wistar rats were randomized into four groups. Group 1 (control group) (n = 10) not exposed to hyperbaric or enhanced oxygen concentrations; group 2 (HBO group) (n = 10) underwent only HBO treatment; group 3 (n = 10) received HBO and intranasal mometasone furoate (10 µl/day); group 4 (n = 10) treated with HBO and diclofenac sodium (10 mg/kg/day ip). Specimens of nasal mucosa were collected after sacrificing and dissection of animals. The specimens were processed for light microscopic evaluation, and then evaluated histopathologically for fibroblastic proliferation and inflammation. Regarding the scores of inflammation, the level of inflammation in the control group was significantly less severe than the other groups (p < 0.05). Evaluation of the fibrosis scores showed that the scores of both groups 2 and 4 were significantly increased (p < 0.05). There were no statistically significant differences between groups 2, 3, and 4 as for fibrosis and inflammation (p > 0.05). Chronic HBO treatment induced mild inflammation of the nasal mucosa. These effects cannot be prevented adequately by administration of nasal steroids and diclofenac.


Assuntos
Anti-Inflamatórios/farmacologia , Diclofenaco/farmacologia , Oxigenoterapia Hiperbárica , Mucosa Nasal/efeitos dos fármacos , Mucosa Nasal/patologia , Pregnadienodiois/farmacologia , Administração Intranasal , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Método Duplo-Cego , Fibrose/etiologia , Fibrose/prevenção & controle , Oxigenoterapia Hiperbárica/efeitos adversos , Inflamação/etiologia , Inflamação/prevenção & controle , Masculino , Furoato de Mometasona , Distribuição Aleatória , Ratos Wistar
11.
Clinics (Sao Paulo) ; 68(11): 1440-5, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24270957

RESUMO

OBJECTIVE: Our aim was to investigate the effects of growth hormone (GH), hyperbaric oxygen and combined therapy on normal and ischemic colonic anastomoses in rats. METHODS: Eighty male Wistar rats were divided into eight groups (n = 10). In the first four groups, non-ischemic colonic anastomosis was performed, whereas in the remaining four groups, ischemic colonic anastomosis was performed. In groups 5, 6, 7, and 8, colonic ischemia was established by ligating 2 cm of the mesocolon on either side of the anastomosis. The control groups (1 and 5) received no treatment. Hyperbaric oxygen therapy was initiated immediately after surgery and continued for 4 days in groups 3 and 4. Groups 2 and 6 received recombinant human growth hormone, whereas groups 4 and 8 received GH and hyperbaric oxygen treatment. Relaparotomy was performed on postoperative day 4, and a perianastomotic colon segment 2 cm in length was excised for the detection of biochemical and mechanical parameters of anastomotic healing and histopathological evaluation. RESULTS: Combined treatment with hyperbaric oxygen and GH increased the mean bursting pressure values in all of the groups, and a statistically significant increase was noted in the ischemic groups compared to the controls (p<0.05). This improvement was more evident in the ischemic and normal groups treated with combined therapy. In addition, a histopathological evaluation of anastomotic neovascularization and collagen deposition showed significant differences among the groups. CONCLUSIONS: Combined treatment with recombinant human growth hormone and hyperbaric oxygen resulted in a favorable therapeutic effect on the healing of ischemic colonic anastomoses.


Assuntos
Colo/cirurgia , Hormônio do Crescimento Humano/uso terapêutico , Oxigenoterapia Hiperbárica/métodos , Isquemia/terapia , Cicatrização/efeitos dos fármacos , Anastomose Cirúrgica , Animais , Colágeno/análise , Colo/irrigação sanguínea , Colo/patologia , Terapia Combinada , Modelos Animais de Doenças , Masculino , Necrose , Neovascularização Fisiológica , Pressão , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Resultado do Tratamento
12.
Clinics ; 68(11): 1440-1445, 1jan. 2013. tab, graf
Artigo em Inglês | LILACS | ID: lil-690628

RESUMO

OBJECTIVE: Our aim was to investigate the effects of growth hormone (GH), hyperbaric oxygen and combined therapy on normal and ischemic colonic anastomoses in rats. METHODS: Eighty male Wistar rats were divided into eight groups (n = 10). In the first four groups, non-ischemic colonic anastomosis was performed, whereas in the remaining four groups, ischemic colonic anastomosis was performed. In groups 5, 6, 7, and 8, colonic ischemia was established by ligating 2 cm of the mesocolon on either side of the anastomosis. The control groups (1 and 5) received no treatment. Hyperbaric oxygen therapy was initiated immediately after surgery and continued for 4 days in groups 3 and 4. Groups 2 and 6 received recombinant human growth hormone, whereas groups 4 and 8 received GH and hyperbaric oxygen treatment. Relaparotomy was performed on postoperative day 4, and a perianastomotic colon segment 2 cm in length was excised for the detection of biochemical and mechanical parameters of anastomotic healing and histopathological evaluation. RESULTS: Combined treatment with hyperbaric oxygen and GH increased the mean bursting pressure values in all of the groups, and a statistically significant increase was noted in the ischemic groups compared to the controls (p<0.05). This improvement was more evident in the ischemic and normal groups treated with combined therapy. In addition, a histopathological evaluation of anastomotic neovascularization and collagen deposition showed significant differences among the groups. CONCLUSIONS: Combined treatment with recombinant human growth hormone and hyperbaric oxygen resulted in a favorable therapeutic effect on the healing of ischemic colonic anastomoses. .


Assuntos
Animais , Masculino , Ratos , Colo/cirurgia , Hormônio do Crescimento Humano/uso terapêutico , Oxigenoterapia Hiperbárica/métodos , Isquemia/terapia , Cicatrização/efeitos dos fármacos , Anastomose Cirúrgica , Terapia Combinada , Colágeno/análise , Colo/irrigação sanguínea , Colo/patologia , Modelos Animais de Doenças , Necrose , Neovascularização Fisiológica , Pressão , Ratos Wistar , Reprodutibilidade dos Testes , Resultado do Tratamento
13.
Brain Res ; 1531: 113-21, 2013 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-23920007

RESUMO

Hyperbaric oxygen (HBO) treatment yields conflicting results on blood-brain barrier (BBB) integrity under various pathological conditions and the effects of HBO on healthy brain is poorly understood. In this experimental study, the effects of HBO on BBB integrity were investigated in comparison with hyperbaric air (HBA) in intact rats. Four sessions of HBA or HBO were applied to intact rats in 24h. BBB integrity was functionally and structurally evaluated by determining extravasation of Evans blue (EB) dye and horseradish peroxidase (HRP) tracers. In immunohistochemical evaluation, relative staining intensity for occludin, a tight junction (TJ) protein, and aquaporin 4 (AQP4), a water-channel protein, was detected in the barrier type of microvessels of brain by image analysis. BBB permeability to EB dye significantly increased in animals in HBO treatment group compared to those in HBA and control groups (p<0.05). The immunoreactivity of occludin, a tight junction protein, remained essentially unaltered in capillaries of hippocampus in all groups. In animals exposed to HBO, AQP4 immunoreactivity significantly increased in parietal cortex compared to those in HBA and control groups (p<0.01). Ultrastructurally, frequent vesicles containing HRP reaction products were observed in capillary endothelial cells in cerebral cortex and hippocampus of rats subjected to both HBA and HBO. Our results indicate that the HBO administration to intact rats increased BBB permeability to both EB and HRP while HBA increased only HRP extravasation in these animals. The results of this study suggest that HBA also impairs the BBB integrity in intact rats as well as HBO.


Assuntos
Ar , Barreira Hematoencefálica/ultraestrutura , Encéfalo/ultraestrutura , Endotélio Vascular/ultraestrutura , Oxigenoterapia Hiperbárica/métodos , Animais , Barreira Hematoencefálica/metabolismo , Encéfalo/metabolismo , Endotélio Vascular/metabolismo , Feminino , Ratos , Ratos Wistar
14.
World J Gastroenterol ; 19(19): 2904-12, 2013 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-23704823

RESUMO

AIM: To investigate the role of growth hormone (GH), hyperbaric oxygen therapy (HBOT) and combined therapy on the intestinal neomucosa formation of the gastric serosa. METHODS: Forty-eight male Wistar-albino rats, weighing 250-280 g, were used in this study. The rats were divided into four groups (n = 12): Group 1, control, gastric serosal patch; Group 2, gastric serosal patch + GH; Group 3, gastric serosal patch + HBOT; and Group 4, gastric serosal patch + GH + HBOT. Abdominal access was achieved through a midline incision, and after the 1-cm-long defect was created in the jejunum, a 1 cm × 1 cm patch of the gastric corpus was anastomosed to the jejunal defect. Venous blood samples were taken to determine the insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein 3 (IGFBP-3) basal levels. HBOT was performed in Groups 3 and 4. In Groups 2 and 4, human GH was given subcutaneously at a dose of 2 mg per kg/d for 28 d, beginning on the operation day. All animals were sacrificed 60 d after surgery. The jejunal segment and the gastric anastomotic area were excised for histological examination. The inflammatory process, granulation, collagen deposition and fibroblast activity at the neomucosa formation were studied and scored. Additionally, the villus density, villus height, and crypt depth were counted and recorded. The measurements of villus height and crypt depth were calculated with an ocular micrometer. New vessel growth was determined by calculatingeach new vessel in a 1 mm(2) area. RESULTS: In the histological comparison of groups, no significant differences were observed between the control group and Groups 2 and 3 with respect to epithelialization, granulation, fibroblastic activity and the inflammatory process, but significant differences were present between the control group and all others groups (Groups 2-4) with respect to angiogenesis (P < 0.01) and collagen deposition (P < 0.05, P < 0.01). Significant differences between the control group and Group 4 were also observed with respect to epithelialization and fibroblastic activity (P < 0.01 and P < 0.05, respectively). There were significant differences in villus density in all of groups compared with the control group (P < 0.05). Crypt depth was significantly greater in Group 4 than in the control group (P < 0.05), but no other groups had deeper crypts. However, villus height was significantly longer in Groups 2 and 4 than in the control group (P < 0.05). The comparison of groups revealed, significant difference between control group and Groups 2 and 4) with respect to the levels of IGF-1 and IGFBP-3 (P < 0.01) 3 wk after the operation. CONCLUSION: HBOT or GH and combined therapy augmented on neomucosal formation. The use of combined therapy produced a synergistic effect on the histological, morphological and functional parameters.


Assuntos
Hormônio do Crescimento Humano/farmacologia , Oxigenoterapia Hiperbárica , Mucosa Intestinal/efeitos dos fármacos , Jejuno/efeitos dos fármacos , Membrana Serosa/efeitos dos fármacos , Síndrome do Intestino Curto/terapia , Estômago/efeitos dos fármacos , Anastomose Cirúrgica , Animais , Terapia Combinada , Modelos Animais de Doenças , Mucosa Gástrica/metabolismo , Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina/sangue , Fator de Crescimento Insulin-Like I/metabolismo , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Mucosa Intestinal/cirurgia , Jejuno/metabolismo , Jejuno/patologia , Jejuno/cirurgia , Masculino , Ratos , Ratos Wistar , Regeneração/efeitos dos fármacos , Membrana Serosa/metabolismo , Membrana Serosa/patologia , Membrana Serosa/cirurgia , Síndrome do Intestino Curto/etiologia , Síndrome do Intestino Curto/metabolismo , Síndrome do Intestino Curto/patologia , Estômago/patologia , Estômago/cirurgia , Fatores de Tempo
15.
Diving Hyperb Med ; 43(4): 232-5, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24510331

RESUMO

Normally pulmonary over-inflation is not an issue during breath-hold diving, in contrast to lung squeeze. Compared with compressed air diving, pulmonary barotrauma is rare in breath-hold diving. Several mechanisms can lead to an increase in intrathoracic pressure in breath-hold diving that may cause alveolar rupture. Here we report three cases of pulmonary barotrauma in breath-hold diving. Using high-resolution chest tomography, bullous damage in Case 1, and pneumomediastinum in Cases 2 and 3 were detected. Transient neurological symptoms in Cases 1 and 2 suggested cerebral arterial gas embolism. The mechanisms that caused intrapulmonary overpressure were, respectively, lung packing ('buccal pumping'), considerable effort and straining at depth, and breathing compressed air at depth and ascending without exhaling. All three cases recovered without specific treatment such as recompression.


Assuntos
Barotrauma/etiologia , Suspensão da Respiração , Lesão Pulmonar/etiologia , Enfisema Mediastínico/etiologia , Adolescente , Adulto , Barotrauma/diagnóstico por imagem , Humanos , Hiperventilação , Lesão Pulmonar/diagnóstico por imagem , Masculino , Enfisema Mediastínico/diagnóstico por imagem , Pescoço , Radiografia , Remissão Espontânea , Enfisema Subcutâneo/etiologia
16.
Brain Res ; 1412: 63-72, 2011 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-21803336

RESUMO

The mechanisms underlying the changes in blood-brain barrier (BBB) integrity in septic encephalopathy are poorly understood. The present study was designed to examine whether hyperbaric oxygen therapy (HBOT) influences the response of BBB to sepsis induced by cecal ligation and puncture (CLP) in rats. Cerebral cortical and hippocampal tissue levels of tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA) and glutathione (GSH) levels were measured. BBB permeability was functionally and structurally evaluated by determining extravasation of Evans blue (EB) and horseradish peroxidase (HRP) tracers, respectively. Immunohistochemistry and western blotting for occludin were performed. HBOT did not alter TNF-α levels in CLP operated rats while a significant decrease was noted when the therapy was subjected to intact rats. MDA levels in animals subjected to CLP plus HBOT were significantly decreased. In septic rats, the decreased GSH levels were significantly increased by HBOT. While HBOT attenuated the increased BBB permeability to EB in rats subjected to CLP (P<0.01), no macroscopic alteration was observed in the enhanced HRP extravasation. An increase in HRP extravasation was also observed by HBOT in intact animals. Occludin immunoreactivity and expression remained essentially unchanged in the brain capillaries of animals in all groups. Ultrastructurally, frequent vesicles containing HRP reaction products were observed in brain capillary endothelial cells of animals treated with CLP and/or HBOT. In conclusion, our results revealed that HBOT did not provide overall protective effects on the BBB integrity in septic conditions and even led to BBB disruption in intact animals.


Assuntos
Barreira Hematoencefálica/metabolismo , Córtex Cerebral/metabolismo , Hipocampo/metabolismo , Oxigenoterapia Hiperbárica , Sepse/metabolismo , Sepse/terapia , Animais , Pressão Sanguínea/fisiologia , Feminino , Glutationa/metabolismo , Malondialdeído/metabolismo , Permeabilidade , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfa/metabolismo
17.
Eur Arch Otorhinolaryngol ; 268(1): 41-7, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20628751

RESUMO

Idiopathic sudden sensorineural hearing loss (ISSNHL) is an otologic emergency with an incidence of about 5-20 per 100,000 of the population per year. There is no universally accepted standard protocol for the treatment of patients with ISSNHL. Hyperbaric oxygen therapy (HBOT), was first reported to improve the outcome following acute inner ear disorders during the late 1960s by both French and German authors. The increase in perilymph oxygenation produced by HBOT provides logical basis for the use of this treatment modality in ISSNHL. We reviewed the records of 97 cases that received HBOT for SSNHL to identify the factors that may affect the treatment outcomes. The effects of age, gender, affected ear, status of the contralateral ear, symptoms associated with hearing loss, presence of a cardiovascular disease, dyslipidemia, history of diabetes mellitus, seasonal factor, smoking, degree of hearing loss, audiogram type, medical treatments provided prior to HBOT, onset time, and number of HBOT sessions were evaluated. The mean hearing gain in all cases after the HBOT was 29.5 dB. The gains were statistically significant in the following cases: early onset of HBOT (p = 0.016), higher number of HBOT sessions (p < 0.01), steroid usage (p = 0.009), low frequency-ascending and total audiogram configuration (p < 0.01) and profound hearing loss (p = 0.011). The success rate was significantly lower in cases with high frequency-descending audiogram configuration (p < 0.001). The most important factor affected the prognosis favorably was found as steroid therapy. This retrospective study and our clinical experience suggest that HBOT has beneficial effects when administered in the early phase of the disease together with steroids. HBOT is a safe practice when used properly by an experienced hyperbaric team. In the treatment of ISSNHL, 20 sessions of HBOT at 2.5 ATA can be tolerated well besides some minor side effects. HBOT should be considered for the cases especially with total or profound hearing loss.


Assuntos
Perda Auditiva Neurossensorial/terapia , Perda Auditiva Súbita/terapia , Oxigenoterapia Hiperbárica/métodos , Adulto , Fatores Etários , Distribuição de Qui-Quadrado , Comorbidade , Feminino , Testes Auditivos , Humanos , Modelos Logísticos , Masculino , Fatores de Risco , Fatores Sexuais , Estatísticas não Paramétricas , Resultado do Tratamento
18.
Aviat Space Environ Med ; 80(4): 414-7, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19378916

RESUMO

Historically, Turkey once had a substantial number of professional sponge divers, a population known for a relatively high incidence of diving-related conditions such as decompression sickness (DCS) and dysbaric osteonecrosis (DON). Sponge diving ended in the mid-1980s when nearly all of the sponges in the Aegean and Mediterranean Seas contracted a bacterial disease and the occupation became unprofitable. We reviewed the records of Turkish sponge divers for information on their level of knowledge, diving equipment, dive profiles, and occupational health problems. Information was collected by: 1) interviewing former sponge divers near Bodrum, where most of them had settled; 2) reviewing the relevant literature; and 3) examining the medical records of sponge divers who underwent recompression treatment. These divers used three types of surface-supplied equipment, including hard helmets, Fernez apparatus, and hookahs; the latter were preferred because they allowed divers the greatest freedom of movement while harvesting sponges underwater. These divers used profiles that we now know involved a high risk for DCS and DON. We were able to access the records of 58 divers who had received recompression treatment. All of the cases involved severe DCS and delays from dive to recompression that averaged 72 h. Complete resolution of symptoms occurred in only 11 cases (19%). Thus, we were able to document the several factors that contributed to the risks in this occupational group, including unsafe dive profiles, resistance to seeking treatment, long delays before recompression, and the fact that recompression treatment used air rather than oxygen.


Assuntos
Doença da Descompressão/epidemiologia , Mergulho/efeitos adversos , Doenças Profissionais/epidemiologia , Osteonecrose/epidemiologia , Adolescente , Adulto , Estudos de Coortes , Doença da Descompressão/fisiopatologia , Humanos , Pessoa de Meia-Idade , Doenças Profissionais/fisiopatologia , Osteonecrose/fisiopatologia , Prevalência , Turquia/epidemiologia , Adulto Jovem
19.
Respir Med ; 102(8): 1145-7, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18571913

RESUMO

BACKGROUND: Air cysts or blebs in the lungs may predispose pulmonary barotrauma (PBT) by causing air trapping when there is a change in environmental pressure. The changes in the environmental pressure are also seen during hyperbaric oxygen treatments (HBOT). AIM: The aim of this study was to determine how patients were evaluated for pulmonary blebs or bullae, and PBT prevalence in different HBOT centers. METHODS: HBOT centers were asked to participate in this study and a questionnaire was send via e-mail. A total of 98 centers responded to our questionnaire. RESULTS: Sixty-five HBOT centers (66.3%) reported that they applied HBOT to the patients with air cysts in their lungs. X-ray was the most widely used screening method for patients with a history of a lung disease. The prevalence of PBT in theses centers was calculated as 0.00045%. CONCLUSIONS: Our survey demonstrated that (1) a significant portion of the HBO centers accept patients with pulmonary bleb or bullae, (2) although insufficient, X-ray is the mostly used screening tool for patients with a history of pulmonary disease and (3) the prevalence of pulmonary barotrauma is very low in HBOT.


Assuntos
Oxigenoterapia Hiperbárica , Pneumopatias/complicações , Barotrauma/epidemiologia , Barotrauma/etiologia , Vesícula/complicações , Vesícula/diagnóstico , Contraindicações , Cistos/complicações , Cistos/diagnóstico , Pesquisas sobre Atenção à Saúde , Humanos , Oxigenoterapia Hiperbárica/métodos , Pneumopatias/diagnóstico , Lesão Pulmonar/epidemiologia , Lesão Pulmonar/etiologia , Prática Profissional/estatística & dados numéricos , Turquia/epidemiologia
20.
Aviat Space Environ Med ; 79(1): 44-6, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18225777

RESUMO

INTRODUCTION: Dysbaric osteonecrosis (DON) is regarded as an occupational disease for caisson workers, commercial, and military divers. Magnetic resonance imaging (MRI) was suggested for the surveillance of military divers for DON. This study aimed to determine the prevalence of DON in Turkish Navy divers. METHODS: The shoulder, hip, and knee joints of 106 male divers were screened for DON with MRI. A questionnaire was used to obtain information about subjects' medical history and diving experience. RESULTS: The mean age of divers was 34.3 +/- 5.8 yr. The divers had spent an average of 12.1 +/- 6.1 yr at their occupation. The average of total hours of diving was 834 +/- 458 h. The maximum diving depth was 53.0 +/- 18.4 m (175 +/- 61 ft) and the average diving depth was 13.3 +/- 8.4 m (44 +/- 28 ft). MRI examinations of divers did not reveal bone lesions consistent with osteonecrosis. DISCUSSION: We concluded that the risk of DON is very low for military divers who strictly obey the decompression rules and who undergo periodic medical examination. The use of MRI for routine screening of military divers is not justified.


Assuntos
Mergulho/efeitos adversos , Militares , Doenças Profissionais/epidemiologia , Osteonecrose/epidemiologia , Adulto , Mergulho/estatística & dados numéricos , Humanos , Estudos Longitudinais , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/diagnóstico , Doenças Profissionais/etiologia , Osteonecrose/diagnóstico , Osteonecrose/etiologia , Prevalência , Turquia/epidemiologia
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