Insights on hazardous metal bioaccessibility, and groundwater impacted by Zn residues from a legacy mine and risk evaluation of adjacent soils.

This study explored the legacy impact of Zinc plant residues (ZPRs) in Kabwe, Zambia, on the environment and human health, particularly in light of the town's reputation for Pb pollution. ZPRs solid samples and groundwater within and around ZPRs zone were collected from the legacy mine, along with soils in a 10 km radius from the mine site. Bioaccessible fractions of Pb and Zn were elucidated by Japanese leaching test (JLT) and simple bioaccessibility extraction test (SBET). Cationic speciation of Pb and Zn from inhalable and ingestible ZPRs particles was investigated via sequential extraction. Groundwater in the ZPRs area showed higher Zn levels (1490 mg/L) compared to Pb (1.7 mg/L). Elevated Zn concentration were facilitated by the presence of soluble Zn sulfates while Pb was constrained due to its precipitation as anglesite. Groundwater sampled outside the ZPRs area was within the Zambia regulatory limits (< 0.5 mg/L for Pb and < 1 mg/L for Zn). Inhalation exposure to < 30 µm dust particles from ZPRs and soils near the mine indicated negligible risk, with < 3% of bioaccessible Pb in artificial lysosomal fluid. Meanwhile, oral intake of ZPRs particles < 250 µm revealed elevated bioaccessible fractions (36% for Pb and 70% for Zn). ZPRs cationic speciation of ingestible particles < 30 µm, 30-75 µm, 75-150 µm and 150-250 µm indicated that the bioaccessible Pb predominantly emanated from labile Pb fractions under gastric conditions with pH < 1. This was due to the dissolution of Pb associated with the exchangeable phase, carbonates and iron/manganese oxides; however, only exchangeable/carbonate Pb was bioaccessible at pH < 2. Hazard quotients indicated increased risks of Pb intoxication through the ingestion of ZPRs and soils near the legacy mine, with higher risks observed in children, emphasizing the need to remediate legacy mine wastes to reduce health risks and protect groundwater through monitoring in mining-affected regions.

Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress.

At a sedimentary site in an old mine site, Miscanthus sinensis formed patches, where Pinus densiflora seedlings could grow better compared with those outside the patches, indicating that M. sinensis would improve P. densiflora seedling establishment. The purpose of this study was to understand the mechanisms by which M. sinensis facilitates the survival of P. densiflora seedlings by considering the soil properties, heavy metal tolerance, and root endophytes in P. densiflora seedlings at the sedimentary site. The sedimentary site, which is a bare ground, contained high concentrations of Fe, indicating that plants should be exposed to Fe and high soil temperature stresses. Measurement of soil temperature revealed that M. sinensis suppressed sharp increases and alternation of soil temperature, resulting in reducing high soil temperature stress in P. densiflora seedlings. To adapt to the Fe stress environment, P. densiflora outside and inside the patches produced Fe detoxicants, including catechin, condensed tannin, and malic acid. Ceratobasidium bicorne and Aquapteridospora sp. were commonly isolated from P. densiflora seedlings outside and inside the patches as root endophytes, which might enhance Fe tolerance in the seedlings. Aquapteridospora sp., which is considered as a dark-septate endophyte (DSE), was also isolated from the roots of M. sinensis, suggesting that M. sinensis might play a source of a root endophyte to P. densiflora seedlings. Ceratobasidium bicorne could be classified into root endophytes showing symbiosis and weak pathogenicity to host plants. Therefore, high soil temperature stress would weaken P. densiflora seedlings, causing root endophytic C. bicorne to appear pathogenic. We suggested that P. densiflora could adapt to the Fe stress environment via producing Fe detoxicants, and M. sinensis would facilitate the establishment of P. densiflora seedlings in the sedimentary site by providing a DSE, Aquapteridospora sp., and maintaining symbiosis of C. bicorne from high soil temperature stress.

Geochemical audit of a historical tailings storage facility in Japan: Acid mine drainage formation, zinc migration and mitigation strategies.

Historical tailings storage facilities (TSFs) are either abandoned or sparsely rehabilitated promoting acid mine drainage (AMD) formation and heavy metal release. To sustainably manage these sites, a geochemical audit coupled with numerical simulation to predict AMD flow paths and heavy metal migration are valuable. In this study, a 40-year-old TSF in Hokkaido, Japan was investigated. Tailings in this historical TSF contain pyrite (FeS2) while its copper (Cu) and zinc (Zn) contents were 1400-6440 mg/kg and 2800-22,300 mg/kg, respectively. Copper and Zn were also easily released in leaching tests because they are partitioned with the exchangeable phase (29% of Zn; 15% of Cu) and oxidizable fraction (25% of Zn; 33% of Cu). Kinetic modeling results attributed AMD formation to the interactions of pyrite and soluble phases in the tailings with oxygenated groundwater, which is supported by the sequential extraction and leaching results. Calibrations of groundwater/AMD flow and solute transport in the 2D reactive transport model were successfully done using hydraulic heads measured on-site and leaching results, respectively. The model forecasted the quality of AMD to deteriorate with time and AMD formation to continue for 1000 years. It also predicted ~24% AMD flux reduction, including lower Zn release with time when recharge reduction interventions are implemented on-site.

Oosporein Produced by Root Endophytic Chaetomium cupreum Promotes the Growth of Host Plant, Miscanthus sinensis, under Aluminum Stress at the Appropriate Concentration.

Chaetomium cupreum, a root endophyte in Miscanthus sinensis, enhances Al tolerance in M. sinensis by changing aluminum (Al) localization and the production of a siderophore, oosporein, which chelates Al for detoxification. Oosporein has various functions, including insecticidal activity, phytotoxicity, antifungal activity, and a siderophore. In our study, we focused on the detoxification effect of oosporein as a siderophore and on the growth of M. sinensis under Al exposure. In addition, the phytotoxicity of oosporein to M. sinensis was confirmed to compare with those in Lactuca sativa and Oryza sativa as control plants. Under Al stress, oosporein promoted plant growth in M. sinensis seedlings at 10 ppm, which was the same concentration as that detected in M. sinensis roots infected with C. cupreum in our previous study. Oosporein also showed low phytotoxicity to M. sinensis compared with L. sativa at even high concentrations of oosporein. These results suggest that the concentration of oosporein in M. sinensis roots would be maintained at the appropriate concentration to detoxify Al and would promote M. sinensis growth under Al stress, although oosporein would show low phytotoxicity to the natural host plant, M. sinensis, compared with the non-host plant, L. sativa.

Solid-phase partitioning and release-retention mechanisms of copper, lead, zinc and arsenic in soils impacted by artisanal and small-scale gold mining (ASGM) activities.

Artisanal and small-scale gold mining (ASGM) operations are major contributors to the Philippines' annual gold (Au) output (at least 60%). Unfortunately, these ASGM activities lacked adequate tailings management strategies, so contamination of the environment is prevalent. In this study, soil contamination with copper (Cu), lead (Pb), zinc (Zn) and arsenic (As) due to ASGM activities in Nabunturan, Davao de Oro, Philippines was investigated. The results showed that ASGM-impacted soils had Cu, Pb, Zn and As up to 3.6, 83, 73 and 68 times higher than background levels, respectively and were classified as 'extremely' polluted (CD = 30-228; PLI = 5.5-34.8). Minerals typically found in porphyry copper-gold ores like pyrite, chalcopyrite, malachite, galena, sphalerite and goethite were identified by XRD and SEM-EDS analyses. Furthermore, sequential extraction results indicate substantial Cu (up to 90%), Pb (up to 50%), Zn (up to 65%) and As (up to 48%) partitioned with strongly adsorbed, weak acid soluble, reducible and oxidisable fractions, which are considered as 'geochemically mobile' phases in the environment. Although very high Pb and Zn were found in ASGM-impacted soils, they were relatively immobile under oxidising conditions around pH 8.5 because of their retention via adsorption to hydrous ferric oxides (HFOs), montmorillonite and kaolinite. In contrast, Cu and As release from the historic ASGM site samples exceeded the environmental limits for Class A and Class C effluents, which could be attributed to the removal of calcite and dolomite by weathering. The enhanced desorption of As at around pH 8.5 also likely contributed to its release from these soils.

Modeling of the groundwater flow system in excavated areas of an abandoned mine.

This study evaluated the assumption that back-filled excavated areas of old mine workings can be modeled as porous media, where groundwater flow is governed by Darcy's law. The Yatani mine, located in Yamagata Prefecture, Japan, was selected for this study because several mining methods were used during its operation and detailed drawings of the excavated areas of the mine are available. The model was calibrated using combinations of hydraulic conductivities (k), with the best-matched case being selected by comparing calculated and measured AMD fluxes. Modeled AMD fluxes along the drainage tunnel (-2 L level) were consistent with measured data when the excavated areas were considered to be porous media with a specific hydraulic conductivity, and the presence of faults and permeability were taken into account. The model also successfully predicted the increasing trend of AMD flux from the shaft to adit mouth. In the numerical model, the back-filled excavated areas were assumed to behave as porous media, which was shown to be a valid assumption in this mine. The model demonstrated that back-filling the excavated areas and drainage tunnel with low permeability materials could reduce the flux of Zn in AMD by up to 61%.

Acid mine drainage sources and hydrogeochemistry at the Yatani mine, Yamagata, Japan: A geochemical and isotopic study.

This paper describes the geochemistry of groundwater and its flow system in the closed Yatani mine in southern Yamagata Prefecture, Japan. The mine is located in a sulfide deposit containing pyrite and has been generating acid mine drainage (AMD). The study was intended to elucidate the formation of AMD and its flow patterns using geological, hydrological, geochemical, and isotopic techniques. The results indicate that AMD is formed by the interaction of groundwater with sulfide minerals, sand slime, and tailings back-filled into excavated mine areas. Groundwater recharge areas were identified on the mountain slope at an elevation of ~900 m. The formation of AMD in the drifts and shaft was more extensive than that in the deeper drainage levels. Principal component analysis was applied to the hydrogeochemical data to identify the causes of AMD formation. The first, second, and third principal components reveal that the increased ion concentrations in mine drainage are a result of water-mineral reactions in excavated mine areas, the contribution of groundwater in deep reductive environments, and isotopic fractionation during precipitation, respectively. A promising method of reducing AMD formation is to prevent contact between dissolved oxygen and sulfide minerals by increasing the drainage level or by filling the shallow underground excavated area with cementitious materials.

Flow and geochemical modeling of drainage from Tomitaka mine, Miyazaki, Japan.

The chemistry and flow of water in the abandoned Tomitaka mine of Miyazaki, western Japan were investigated. This mine is located in a non-ferrous metal deposit and acid mine drainage issues from it. The study was undertaken to estimate the quantities of mine drainage that needs to be treated in order to avoid acidification of local rivers, taking into account seasonal variations in rainfall. Numerical models aimed to reproduce observed water levels and fluxes and chemical variations of groundwater and mine drainage. Rock-water interactions that may explain the observed variations in water chemistry are proposed. The results show that: (1) rain water infiltrates into the deeper bedrock through a highly permeable zone formed largely by stopes that are partially filled with spoil from excavations (ore minerals and host rocks); (2) the water becomes acidic (pH from 3 to 4) as dissolved oxygen oxidizes pyrite; (3) along the flow path through the rocks, the redox potential of the water becomes reducing, such that pyrite becomes stable and pH of the mine drainage becomes neutral; and (4) upon leaving the mine, the drainage becomes acidic again due to oxidation of pyrite in the rocks. The present numerical model with considering of the geochemical characteristics can simulate the main variations in groundwater flow and water levels in and around the Tomitaka mine, and apply to the future treatment of the mine drainage.