Antibacterial effects of silver diamine fluoride, potassium iodide and nanosilver fluoride on dual-species biofilm.

OBJECTIVES: This study aims to evaluate antibacterial effects of silver diamine fluoride (SDF), SDF/potassium iodide (KI), and nanosilver fluoride (NSF). METHODS: Antimicrobial activity of sterile saline, 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), SDF, SDF/KI, NSF, and KI solutions against Streptococcus mutans and Lactobacillus casei was assessed through disc diffusion tests. A dual-species biofilm of S. mutans-L. casei was formed on 48 enamel samples, divided into six groups (n = 8). Group 1 was treated with sterile saline, Group 2 with 5% NaOCl, Group 3 with 2% CHX, Group 4 with SDF, Group 5 with SDF/KI, and Group 6 with NSF. The samples were analysed using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Statistical analysis utilized Shapiro-Wilk and Kruskal-Wallis tests and multiple comparisons were conducted using Dunn test. RESULTS: SDF, SDF/KI, and NaOCl displayed significantly higher antibacterial activity against dual-species biofilm compared to NSF and CHX (p < 0.050). CONCLUSIONS: In conclusion, SDF and SDF/KI demonstrated greater antibacterial activity than NSF. SDF's antibacterial activity was unaffected by KI. Further research is needed to determine the appropriate content and concentration for achieving effective antibacterial activity with NSF. CLINICAL SIGNIFICANCE: The use of silver-containing materials is increasing in popularity within pediatric dentistry. In this study, an endeavor has been made to assist pediatric dentists in determining which solution might be more advantageous for preventing caries.

Assessment of oral bacteria potentially associated with the mobile microbiome in children with congenital heart disease.

In this case-control study, we aimed to investigate the specific oral pathogens potentially associated with the mobile microbiome in children with congenital heart disease (CHD). Caries, oral hygiene and gingival indices were evaluated in 20 children with CHD and a healthy control group, and venous blood samples and saliva were collected. Using quantitative polymerase chain reaction (qPCR), blood samples were analyzed for the presence of bacterial DNA to determine the mobile microbiome, and saliva samples were analyzed to identify and quantify target microorganisms, including Streptococcus mutans (Sm) and its serotype k (Smk), Fusobacterium. nucleatum (Fn), Porphyromonas gingivalis (Pg), Scardovia wiggsiae (Sw) and Aggregitibacter actinomycetemcomitans (Aa) and its JP2 clone (JP2). The findings were analyzed by Mann Whitney U, chi-square, Fisher's exact and Spearman's Correlation tests. Bacterial DNA was identified in two blood samples. No significant differences were found between the groups regarding the presence and counts of bacteria in saliva. However, the CHD group exhibited significantly lower caries and higher gingival index scores than the control group. The presence of Pg and Aa were significantly associated with higher gingival index scores. Sm and Smk counts were significantly correlated with caries experience. A positive correlation was found between Fn and total bacteria counts. In conclusion, the mobile microbiome, which has been proposed as a potential marker of dysbiosis at distant sites, was very rare in our pediatric population. The counts of target microorganisms which are potentially associated with the mobile microbiome did not differ in children with CHD and healthy children.

Antibacterial effects of dentifrices against Streptococcus mutans in children: a comparative in vitro study.

Fluoridated dentifrices have antibacterial effects on children's teeth. On the other hand, the side effects encountered with the use of them have led researchers to look for safe alternatives. This study aimed to determine the antibacterial effect of different commercially available fluoride-free dentifrices on Streptococcus mutans (S. mutans) in comparison with different concentrations of fluoridated dentifrices. Study groups comprised of fluoride-free dentifrices, which contain Probiotic (Activated Charcoal Probiotic Dentifrice-Group P), Aloe Vera-Group AV and Salivary Proteins-Group SP. Fluoridated dentifrices containing 1450 ppm fluoride-Control Group 1 and 500 ppm fluoride-Control Group 2 served as control groups. Antibacterial activity was assessed by Minimum Inhibitory Concentrations and agar well diffusion assays on S. mutans. Biofilm inhibition assay was performed with dentifrices, which had antibacterial activities, and a negative control phosphate-buffered saline (Group PBS) on sterile hydroxyapatite discs against S. mutans. Statistical evaluation was performed. Only group AV showed an antibacterial effect on S. mutans, while control groups showed a similar antibacterial effect. The mean number of viable bacteria present in S. mutans biofilm in Control Group 1 and 2 and Group AV were statistically significantly lower than that in Group PBS, but there were no statistically significant differences between Control Groups and Group AV. Antibacterial activity of commercial dentifrices against S. mutans may be exerted by antibacterial components other than fluoride. Aloe vera-containing toothpaste showed an antibacterial effect on S. mutans, although not as much as the fluoride-containing toothpastes in the control groups. However, further in vivo and long-term studies are required.

A comparative evaluation of the effects of respiratory diseases on dental caries.

PURPOSE: The aim of this study is to evaluate the susceptibility of patients suffering from asthma and chronic obstructive pulmonary disease (COPD) to dental caries by analyzing the physical, chemical, and microbiological characteristics of saliva, which are influenced by the medications they use. METHODS: A cohort of 104 individuals, spanning from 18 to 70 years of age, underwent a meticulous categorization based on their unique medical profiles and prescribed medication routines. Subsequently, a comprehensive evaluation was conducted to elucidate potential risk factors associated with dental caries. Alongside the assessment of decayed, missing, and filled teeth (DMFT index), decayed, missing, and filled surfaces (DMFS index), and Green and Vermillion Oral Hygiene Index-Simplified (G&V OHI-S) values, measurements were performed to gauge salivary flow rate, buffering capacity, and the presence of S. mutans, L. casei, S. aureus, and C. albicans. The acquired data were then inputted into the Cariogram software, enabling the derivation of personalized caries risk profiles for each individual. RESULTS: The diseased group exhibited significantly elevated levels of DMFT, DMFS, and G&V OHI-S values in comparison to the control group (p < 0.01). Moreover, the caries risk levels derived from the Cariogram were found to be significantly higher in patients diagnosed with asthma and COPD (p < 0.01). Notably, no substantial distinction was observed between these two experimental groups. Furthermore, it was discerned that COPD patients utilizing two or three distinct medications did not display any discernible variation in terms of their susceptibility to dental caries (p > 0.05). CONCLUSION: Asthma and COPD patients exhibit an increased susceptibility to dental caries as a result of their medication regimens. Hence, it is highly advisable for these individuals to demonstrate heightened vigilance in terms of oral hygiene practices and seek regular dental check-ups for continuous monitoring and preventive care.

Antibacterial effects of saliva substitutes containing lysozyme or lactoferrin against Streptococcus mutans.

OBJECTIVE: To determine the antibacterial effects of different saliva-substitutes-containing-lysozyme(LYZ) or-lactoferrin(LF) on Streptococcus mutans(S. mutans) in comparison with human saliva. DESIGN: In vitro wound-healing assay was performed with L929 mouse fibroblast cell line by using various concentrations of LYZ and LF to determine optimum concentrations and to confirm do not show any cytotoxicity of proteins according to cell culture studies. Antibacterial effect was assessed by determining Minimum Inhibitory Concentrations for all groups on S.mutans. Bacterial adhesion of S. mutans for 4 h on hydroxyapatite(HAP) discs after application of different saliva substitutes was evaluated. The formulations were:saliva-substitute(Group SS);saliva-substitute-containing-Lactoferrin(Group SSLF);saliva-substitute-containing-Lysozyme(Group SSLYZ). Human saliva was control group(Group HS). RESULTS: In vitro wound healing assay results showed that, when added into the cell culture media, LYZ and LF significantly increase 48 -h scratch wound closure compared to the cell culture media(p < 0.0001). At the end of second day, samples treated with both between 2.5-100 µg/mL LF and 5-200 µg/mL LYZ were found to have significant wound healing effect(p < 001). It was observed that saliva-substitutes-containing-LYZ or-LF had antibacterial effects on S.mutans. Bacterial adhesion on HAP discs was observed significantly higher in control group than in study groups. The amount of adhered S. mutans was significantly higher in Group SS than other study groups(p < 0.0001). However, no statistically significant difference was found between the number of bacteria adhered to HAP discs between SSLYZ and SSLF groups(p > 0.05). CONCLUSIONS: The study of cell viability and wound healing was great significance in the optimum concentrations of LYZ and LF. Among formulations, saliva-substitutes-containing-LYZ or-LF exhibited higher inhibitory effect on S.mutans.

Effect of different beverages on surface properties and cariogenic biofilm formation of composite resin materials.

The consumption of certain beverages may affect the physical and biological properties of resin composites (RCs) according to type. This in vitro study aimed to evaluate the surface properties and cariogenic biofilm formation in microhybrid and nanohybrid RCs after immersion in different beverages. The effects of four beverages (distilled water-control, tea, coffee, and cola) on two RCs (microhybrid and nanohybrid) were evaluated. Changes in the surface properties were evaluated for each group using surface roughness measurement (n = 10), scanning electron microscopy (SEM) (n = 4) observation, and energy-dispersive X-ray spectroscopy (EDX) (n = 5) analysis. In vitro Streptococcus mutans biofilm formation on the specimens of each group was determined using confocal laser scanning microscopy and SEM analysis (n = 14). The data were analyzed using two-way analysis of variance, with Bonferroni as a post-hoc test and Pearson's correlation (p < .05). Microhybrid RC presented more surface roughness (p = .014) and cariogenic biofilm formation (p = .040). The surface roughness (F = 0.733, p = .536) and cariogenic biofilm formation (F = 1.685, p = .181) values were not affected by the beverages. However, according to qualitative SEM and EDX measurements, these parameters varied depending on the beverage groups. No correlation was found between surface roughness and cariogenic biofilm formation (r = 0.135, p = .287). Microhybrid RCs had a rougher surface and a higher amount of cariogenic biofilm formation than nanohybrid RCs after being subjected to different beverages.

Oral microbiota and dental caries data from monozygotic and dizygotic twin children.

There are recent studies which aimed to detect the inheritance on the etiology of dental caries exploring oral composition. We present data on the oral microbiota and its relation with dental caries and other factors in monozygotic (MZ) and dizygotic (DZ) twin children. Following clinical investigation, DNA samples were collected and isolated from saliva of 198 patients (49 MZ and 50 DZ twins) with an average age of 9.7 ± 2.7 years. Salivary bacterial microbiota analysis was performed using high throughput amplicon sequencing method targeting V3-V4 region of the 16S rRNA gene. A total of 8,297,859 raw reads corresponding to 41,908 reads per sample were obtained on average. The QIIME2-deblur workflow was used for 16S rRNA amplicon analysis. Microbiome similarity analyses between twins (based on Bray-Curtis dissimilarity, weighted and unweighted Unifrac distances) showed that monozygotic twins share more bacterial microbial content compared to dizygotic twins. This is a large microbial community dataset of MZ and DZ twins with or without dental findings which can be further used for children oral microbiome profile explorations.

Effect of different polishing techniques for composite resin materials on surface properties and bacterial biofilm formation.

OBJECTIVES: Both direct and indirect techniques are used for composite resin material (CRM) restorations. Polishing processes are needed in both techniques after intraoral adjustment. However, it is unclear as to which polishing technique should be preferred with respect to decreasing biofilm. The purpose of thisin vitro study was to evaluate the surface properties and Streptococcus mutans biofilm formation on direct and indirect CRMs after using different polishing techniques. METHODS: Two CRMs (direct and indirect) and four polishing techniques (aluminium oxide discs, diamond polishing paste, aluminium oxide polishing paste, and silicon carbide brush) were evaluated. The specimens were prepared for taking scanning electron microscopy images (n = 2) and determining surface roughness, surface free energy, and bacterial biofilm formation (BBF) with colony-forming unit counting and confocal laser scanning microscopy assays (n = 7). The data were analysed using two-way analysis of variance with Bonferroni as a post hoc test and Pearson's correlation (p < .05). RESULTS: The surface roughness values in the control group were higher than those in the diamond polishing paste group (p = 0.025), but the values in the aluminium oxide polishing paste and silicon carbide brush groups were comparable with those in the control group (p =  0.156 and p =  1.000, respectively). The highest surface free energy values were recorded in the silicon carbide brush group (p < 0.001), whereas there were no differences found among the other groups (p > 0.05). The highest BBF was seen in the silicon carbide brush (p <  0.001) and direct CRM (p < 0.001) groups. CONCLUSION: BBF on the surface of direct CRMs differed from that on indirect CRMs after polishing the surface. The tested polishing techniques significantly influenced surface properties and BBF. CLINICAL SIGNIFICANCE: In situations that require the intraoral adjustment of CRMs, polishing with a diamond polishing paste seems to be a good option to polish the surface of both direct and indirect CRMs because the diamond polishing paste results better in terms of decreasing biofilm formation and improving surface properties.

Bactericidal Effect of 2780 nm Er,Cr:YSGG Laser Combined with 940 nm Diode Laser in Enterococcus faecalis Elimination: A Comparative Study.

Objective: This study aims to compare the bactericidal efficacy of different Er,Cr:YSGG disinfection methods, particularly combined application of Er,Cr:YSGG and Diode laser. Background: Root canal disinfection is important for long-term endodontic success. Different laser systems are proposed as alternatives to conventional techniques in bacterial elimination. Materials and methods: Ninety-five straight, single-rooted premolars with similar canal dimensions were selected. After the mechanical preparation, the samples were sterilized, and root canals were infected with Enterococcus faecalis. The samples were randomly divided into five groups (n = 15): 5% NaOCl, Er,Cr:YSGG, Er,Cr:YSGG +5% NaOCl, Er,Cr:YSGG + Diode, and control group. Results: The maximal bacterial elimination was observed in Er,Cr:YSGG + NaOCl group (p < 0.005). More bacteria were found in Er,Cr:YSGG and Er,Cr:YSGG + Diode groups compared with 5% NaOCl group (p < 0.005). Marginal significance was detected between the Er,Cr:YSGG group and the Er,Cr:YSGG + Diode group (p = 0.091). Conclusions: The most successful E. faecalis elimination was obtained from laser-activated irrigation group. As combined application of Er,Cr:YSGG and Diode laser gives suggestive results, further studies with larger sample sizes are needed to clarify the outcome.

Oral microbial dysbiosis in patients with Kostmann syndrome.

PURPOSE: The oral microbiome is maintained by host- and microbe-derived factors. A shift in microbial composition, as a result of diseases related to the immune system, is the most important step in the development of oral and dental diseases. The aim of this study was to investigate the oral microbial composition of patients with Kostmann syndrome, who have severe neutropenia, compared with healthy children. METHODOLOGY: A group of nine Kostmann syndrome patients and a group of nine healthy controls participated. After clinical investigation, DNA from stimulated saliva specimens was examined by high-throughput sequencing of the V3-V4 hypervariable region of the 16S rRNA gene using Illumina sequencing. The QIIME software package was used for 16 S rRNA amplicon analysis, while the Greengenes database was used for taxonomic classification. RESULTS: The periodontal pocket depths, plaque indices and bleeding-on-probing percentages and caries status on the deciduous teeth of the patients with Kostmann syndrome were statistically higher than those for the healthy controls. Patients with Kostmann syndrome had significantly lower bacterial diversity as compared to the controls. The presence of Firmicutes was statistically higher in patients with Kostmann syndrome, while that for Proteobacteria was higher in samples from the healthy controls (P<0.05). Streptococcus, Rothia, Granulicatella, Actinomyces, and genera from the family Gemellaceae were present as the core microbiome (abundance >1 % in at least 75  % of samples) in all groups, whereas the genus Porphyromonas was only detected as a member of the core microbiome in Kostmann patients. CONCLUSIONS: The evidence of lower bacterial diversity and differences in microbial profile for patients with Kostmann syndrome not only shows the impact of immune system-related diseases on oral microbiota, but also endorses the ecological plaque hypothesis proposed for the aetiology of oral diseases such as dental caries and periodontitis.

Microbial Profile and Dental Caries in Cleft Lip and Palate Babies Between 0 and 3 Years Old.

OBJECTIVE: The aim of this study was to examine the microbiological changes in newborn babies with cleft lip palate from birth up to age 3 and to correlate them with their caries levels and mothers' microbiological data and to compare with normal infants. BASIC RESEARCH DESIGN: Prospective. SETTINGS: Marmara University, Faculty of Dentistry, Pediatric Dentistry Clinic, and Sisli Hamidiye Etfal Education and Research Hospital New Born Clinic. PATIENTS/PARTICIPANTS: Cleft lip palate (n = 21) and healthy (n = 13) newborns and their mothers. MATERIAL AND METHODS: Intraoral samples were taken from babies in each group at least 3 times over the 3 years. Saliva samples of the mothers were collected just after the birth of the babies and examined microbiologically. Dental caries was noted as either present or absent. RESULTS: The most frequent microorganisms were candida, found at birth (n = 9, 42%) in cleft palate with or without cleft lip (CP±L) group. The number of babies infected with Lactobacilli were found to be significantly higher in the CP±L group than in the control group at birth ( P = .029) and after eruption of the first primary tooth ( P = .030). Mutans Streptococci were found in 10% of babies with CP±L at birth. Initial caries was identified in 20% of the babies with an oral cleft compared with 0% of the controls after eruption of the first primary incisors. CONCLUSION: The results show that the CP±L babies must be considered as a group with an increased caries risk.

Pathogen profile and MMP-3 levels in areas with varied attachment loss in generalized aggressive and chronic periodontitis.

INTRODUCTION: The progression of periodontitis depends on the changes in bone and connective tissue homeostasis and the imbalance of the biofilm and the host immunoinflammatory response, particularly matrix metalloproteinases (MMP). AIM OF THE STUDY: To assess the probable relation between subgingival anaerobic flora and the expression of MMP-3 in patients with generalized aggressive periodontitis (AgP), chronic periodontitis (CP) and healthy subjects, and to evaluate these levels according to varied tissue loss severity. MATERIAL AND METHODS: The plaque index (PI), gingival index (GI), probing depth (PD) and clinical attachment levels (CAL) were evaluated. MMP levels obtained from gingival sulcus fluid (GCF) were measured with Enzyme Linked Immuno Assay (ELISA). The bacterial counts were determined with Parocheck®. RESULTS: Higher levels of MMP-3 in patients with AgP compared to subjects with CP and healthy individuals were observed. The microorganisms responsible of possible tissue destruction in both AgP and CP are red complex bacteria. T. denticola, T. forsythia, P. intermedia and F. nucleatum show positive correlation with MMP-3 levels. CONCLUSIONS: MMP-3 is a biomarker associated with AgP, and red complex bacteria levels are correlated with increasing periodontal tissue loss in both periodontitis forms. The diagnosis of aggressive periodontitis, or site-specific treatment strategies can be orchestrated based on the evaluation of MMP-3 and the bacterial counts in patients with periodontitis.

Assessment of the endodontic microbiota of abscessed primary teeth using microarray technology.

CONTEXT: Knowledge of the microbial composition of abscessed primary tooth is limited. AIM: The aim was to investigate the presence of 10 oral bacterial species in samples from abscessed primary tooth root canals using microarray technology and to determine their association with clinical findings. SUBJECTS AND METHODS: The samples were collected from root canals of 20 primary molars with acute primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (ParoCheck®). Clinical parameters included the presence of spontaneous pain, mobility, percussion sensitivity and swelling. STATISTICAL ANALYSIS: Data were statistically analyzed by Student' t-test, Fisher's exact Chi-square test, Freeman-Halton-Fisher's exact test, and Spearman's rho correlation analysis. RESULTS: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequent bacterium (100%), followed by Parvimonas micra (65%), Provetella intermedia (45%), and Treponema denticola (45%). According to paired bacterial combinations, F. nucleatum was significantly positively correlated with P. intermedia and P. micra (P < 0.05). T. denticola was significantly positively correlated with Porphyromonas gingivalis, Tannerella forsythia, Campylobacter rectus, and P. micra, while it was negatively correlated with Eikenella corrodens (P < 0.05). No statistically significant relationships were found between the presence of any bacteria and clinical findings. CONCLUSION: Microarray technology used in this study has demonstrated the presence of various bacteria with varying proportions in the root canals of abscessed primary teeth. The results regarding the high rate of certain bacterial combinations suggest the enhanced pathogenicity due to additive or synergistic effects of these microbial combinations.

Effects of Taper Angle and Sealant Agents on Bacterial Leakage Along the Implant-Abutment Interface: An In Vitro Study Under Loaded Conditions.

PURPOSE: The aim of this study was to compare the bacterial leakage of conical internal connection implants with different taper angles (5.4, 12, 45, and 60 degrees) and examine the efficiency of a disinfectant agent and a silicone sealant agent in the prevention of bacterial leakage under loaded conditions. MATERIALS AND METHODS: Twenty-one implant-abutment connections were studied from each implant system (Ankylos Implants, Dentsply; Bego Semados S Implants, Bego; Trias Implants, Servo-Dental; DTI Implants, DTI), for a total of 84 implants. Each system's implants were divided into three groups as follows: unsealed (control), 2% chlorhexidine gel-sealed, or silicone-sealed (n = 7 for each group). The insertion torque was applied to each abutment screw according to the manufacturers' recommendation. The specimens were partially immersed in an 8-mm E faecalis suspension. A cyclic load of 50 N was applied for a total of 500,000 cycles at 1 Hz to the specimens. Following disconnection of dental implants and abutments, microbial samples were taken from the inner threaded surface of the implants, plated, and counted under appropriate conditions. RESULTS: There were no statistically significant differences in frequency of bacterial leakage and leaked bacterial counts among the four types of connections in all groups (P > .05). The statistically significant differences were found between sealant agents and control groups in four different connection types in terms of the amount of leaked bacteria (P < .05). There was no significant difference between the amount of leaked bacteria for four connection types when comparing the chlorhexidine and silicone sealant agents (P > .05). CONCLUSION: Differences in taper angles in the internal conical connections had no significant effect on leaked bacterial counts or the frequency of bacterial contamination under dynamic loading. The application of 2% chlorhexidine gel or a silicone sealant can reduce the leaked bacterial counts and reduce the frequency of bacterial leakage.

Comparison of different final irrigant agitation techniques for the removal of Enterococcus faecalis biofilms from root canals: an in vitro study / Comparação de diferentes técnicas finais de agitação de irrigantes para a remoção de biofilmes de Enterococcus faecalis de canais radiculares: estudo in vitro

Objective: The aim of this in vitro study was to compare the effectiveness of different final irrigant agitation techniques in the removal of Enterococcus faecalis biofilms from root canals. Material and Methods: In total, the root canals of 85 extracted single-rooted human maxillary incisors teeth were prepared using the Revo-S system to a 40/06 size. The apical foramen of each tooth was sealed by light-cured resin composite material to obstruct bacterial leakage. The specimens were sterilized in an autoclave at 121°C for 15 min and stored until further use. All teeth except five (negative control group) were inoculated with Enterococcus faecalis and incubated in a CO2 chamber at 37°C for 7 days; the trypticase soy broth was changed every 2 days. For the determination of possible biofilm formation, five of the 80 teeth were randomly selected as a positive control group; one tooth of positive control group was analysed for biofilm development by scanning electron microscope (SEM) and these teeth received no final irrigant agitation procedure. Then, the remaining 75 teeth were randomly divided into five test groups (n=15 each) and were sequentially irrigated with 5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid and 5% NaOCl. Following each irrigant application, different final irrigant agitation techniques were introduced for 60 s (3×20-s sessions). Group 1 received manual­ dynamic agitation, group 2 received passive ultrasonic agitation (PUI), group 3 received EndoActivator agitation, group 4 received photoninitiated photoacoustic streaming (PIPS) with the Er:YAG laser and group 5 received conventional syringe irrigation. Colony-forming units (CFUs) were counted in samples from the positive control and test groups. Data were analysed using Kruskal­ Wallis and post-hoc Mann­Whitney U multiple comparison tests. Results: E. faecalis elimination was significantly better in the experimental groups than in the positive control groups (p < 0.001). Manual­dynamic agitation and conventional syringe irrigation, with no significant differences between the two groups. Conclusion: Essentially, CFU reduction was significantly greater in the PUI, EndoActivator and PIPS groups than in the manual­dynamic agitation and conventional syringe irrigation groups (p <0.001) , with no significant differences among the former three groups. (AU)

Objetivo: O objetivo deste estudo in vitro foi comparar a eficácia de diferentes técnicas finais de agitação de irrigantes na remoção de biofilmes de Enterococcus faecalis de canais radiculares. Material e Métodos: No total, os canais radiculares de 85 dentes incisivos superiores unirradiculares humanos extraídos foram preparados usando o sistema Revo-S para um tamanho 40/06. O forame apical de cada dente foi selado por material compósito de resina fotopolimerizável para obstruir o vazamento bacteriano. Os espécimes foram esterilizados em autoclave a 121 ° C por 15 min e armazenados até uso posterior. Todos os dentes, exceto cinco (grupo controle negativo), foram inoculados com Enterococcus faecalis e incubados em câmara de CO2 a 37 ° C por 7 dias; o caldo de soja tripticase foi trocado a cada 2 dias. Para a determinação da possível formação de biofilme, cinco dos 80 dentes foram selecionados aleatoriamente como grupo controle positivo; um dos dentes do grupo controle positivo foi analisado para o desenvolvimento do biofilme por microscopia eletrônica de varredura (MEV) e estes dentes não receberam nenhum procedimento final de agitação irrigante. Em seguida, os 75 dentes restantes foram aleatoriamente divididos em cinco gruposteste (n = 15 cada) e irrigados sequencialmente com hipoclorito de sódio a 5% (NaOCl), ácido etilenodiaminotetracético a 17% e NaOCl a 5%. Após cada aplicação de irrigantes, diferentes técnicas finais de agitação foram introduzidas por 60 s (3 x 20 s sessões). Grupo 1 recebeu agitação manual-dinâmica, grupo 2 recebeu agitação ultra-sônica passiva (PUI), grupo 3 recebeu agitação EndoActivator, grupo 4 recebeu fotoacústica iniciada por fóton (PIPS) com o laser Er: YAG e grupo 5 recebeu irrigação convencional com seringa. As unidades formadoras de colônia (CFUs) foram contadas em amostras dos grupos controle positivo e teste. Os dados foram analisados utilizando testes de comparação múltipla Kruskal-Wallis e post-hoc Mann-Whitney U. Resultados: A eliminação de E. faecalis foi significativamente melhor nos grupos experimentais do que nos grupos de controle positivo (p < 0,001). Agitação manual-dinâmica e irrigação com seringa convencional, sem diferenças significativas entre os dois grupos. Conclusão: Essencialmente, a redução de UFC foi significativamente maior nos grupos PUI, EndoActivator e PIPS do que nos grupos de agitação manual-dinâmica e de seringa convencional (p < 0,001), sem diferenças significativas entre os três grupos anteriores (AU)

Effect of two different polishing systems on fluoride release, surface roughness and bacterial adhesion of newly developed restorative materials.

OBJECTIVES: To evaluate the effects of two different polishing systems on fluoride release, surface roughness and bacterial adhesion of five restorative materials MATERIALS AND METHODS: The study groups were comprised of five different restorative materials, Beautifil II (B); GCP Glass Fill (G); Amalgomer CR (A); Dyract XP (D); Fuji IX GP (F) and 21 specimens were prepared from each material. Each group was divided into three subgroups according to the polishing system: Mylar (control) (C), Sof-lex (S), and Enhance-Pogo (EP). The amount of fluoride release was measured using a fluoride ion-selective electrode and surface roughness was investigated with a profilometer. Bacterial adhesion on the materials was evaluated by optical density readouts for S.mutans on a spectrophotometer. RESULTS: The highest amount of fluoride was released from specimens in the S subgroup of group G during all measurement days. Surface roughness values were significantly lower in subgroup C than the other polishing systems in all study groups except group G (P < .05). Group A displayed significantly higher surface roughness values than the other material groups in both subgroups (S and EP) (P < .01). Highest bacterial adhesion was observed in the EP subgroup of group A. CONCLUSIONS: Polishing promoted a significant increase of fluoride release on restorative materials especially in glass ionomer-based materials. CLINICAL SIGNIFICANCE: This article stated that polishing promoted a significant increase of fluoride release on restorative materials especially in glass ionomer-based materials. Further, proper polishing systems must be chosen according to the structure and composition of materials to provide the best clinical benefits in terms of fluoride release, surface roughness and bacterial adhesion.

Presence of Aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children with congenital heart disease.

AIM: The purpose of this study was to analyze the presence of Aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children requiring cardiac surgery in Istanbul, Turkey. SUBJECTS AND METHODS: Twenty-five patients (mean age: 6.24 ± 2.93) undergoing surgery for congenital heart defects (CHDs) and an age/gender-matched control group of 25 healthy children were enrolled in the study. Saliva samples were collected from all children; plaque index (PI) and gingival index (GI) were also determined. In CHD group, cardiac tissue samples were received during surgery. All samples were evaluated for the presence of A. actinomycetemcomitans and its highly leukotoxic JP2 clonal strains using polymerase chain reaction. The findings were analyzed by Mann-Whitney U, Chi-square, and Fisher's exact tests. RESULTS: No significant differences were found in PI and GI values between the groups. A. actinomycetemcomitans was not detected in cardiac tissue samples. A. actinomycetemcomitans in saliva was detected in 2 (8%) of the CHD and 5 (20%) of the control children (p > 0.05). A. actinomycetemcomitans JP2 clonal strains were determined from 1 (4%) of the control group while it was not determined from the samples of the CHD group. CONCLUSIONS: Early colonization of A. actinomycetemcomitans in oral cavities could be assessed as a risk marker for periodontal disease. Periodontal pathogens may enter bloodstream through bacteremia; thus, the presence of periodontal pathogens in the oral cavity of children should be assessed as a risk marker for cardiac diseases in older ages.

A randomized clinical trial of an adjunct diode laser application for the nonsurgical treatment of peri-implantitis.

OBJECTIVE: In this radiographic and microbiologic split-mouth clinical trial, efficacy of a diode laser as an adjunct to conventional scaling in the nonsurgical treatment of peri-implantitis was investigated. BACKGROUND DATA: Eradication of pathogenic bacteria and infected sulcular epithelium presents a significant challenge in the nonsurgical treatment of peri-implantitis. MATERIALS AND METHODS: Ten patients (mean age, 55.1 years; SD, 11.4) with 48 two piece, rough-surface implants and diagnosed with peri-implantitis were recruited (NCT02362854). In addition to conventional scaling and debridement (control group), crevicular sulci and the corresponding surfaces of 24 random implants were lased by a diode laser running at 1.0 W power at the pulsed mode (λ, 810 nm; energy density, 3 J/cm(2); time, 1 min; power density, 400 mW/cm2; energy, 1.5 J; and spot diameter, 1 mm); (laser group). Healing was assessed via periodontal indexes (baseline and after 1 and 6 months after the intervention), microbiologic specimens (baseline and after 1 month), and radiographs (baseline and after 6 months). RESULTS: Baseline mean pocket depths (4.71, SD, 0.67; and 4.38, SD 0.42 mm) and marginal bone loss (2.71, SD 0.11; and 2.88, SD 0.18 mm) were similar (p = 0.09 and p = 0.12) between the control and laser groups, respectively. After 6 months, the laser group revealed higher marginal bone loss (2.79, SD 0.48) than the control groups (2.63, SD 0.53) (p < 0.0001). However, in both groups, the microbiota of the implants was found unchanged after 1 month. CONCLUSIONS: In this clinical trial, adjunct use of diode laser did not yield any additional positive influence on the peri-implant healing compared with conventional scaling alone.

An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: An in vitro study.

OBJECTIVE: The aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; Biodentine, mineral trioxide aggregate (MTA) Angelus, and Dycal against Enterococcus faecalis and their durability with time. MATERIALS AND METHODS: Direct contact test was used for the assessment. Three sets of sealers were mixed and placed on microtiter plate wells: One set was used within 20 min of recommended setting time while others were used after 24-h and 1-week. E. faecalis suspension was placed directly on the materials for 1 h and then transferred to another plate with fresh media. Nine wells of bacteria without the tested cements served as the positive control. One well of the tested cements without bacteria served as the negative control. Bacterial growth was evaluated by a temperature-controlled microplate spectrophotometer for 1-h intervals among 24 h. Data were analyzed using Kruskal-Wallis test. RESULTS: All tested materials showed less bacterial density than the control group. MTA, Biodentine, and Dycal showed significantly higher bacterial density than the control group in freshly mixed samples (P < 0.05). And MTA showed significantly higher antibacterial activity than Dycal (P < 0.05). In 24 h, materials did not show any differences (P > 0.05). MTA and Biodentine samples showed significant differences than Dycal; MTA also showed higher antibacterial activity than control in 1-week samples (P < 0.05). CONCLUSION: While freshly mixed MTA showed the best antibacterial activity over time, Biodentine had shown similar antibacterial activity to MTA.