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1.
Foods ; 12(11)2023 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-37297428

RESUMO

According to the United Nations, approximately one-third of the food produced for human consumption is wasted. The actual linear "Take-Make-Dispose" model is nowadays obsolete and uneconomical for societies and the environment, while circular thinking in production systems and its effective adoption offers new opportunities and benefits. Following the "Waste Framework Directive" (2008/98/CE), the European Green Deal, and the actual Circular Economy Action Plan, when prevention is not possible, recovering an unavoidable food waste as a by-product represents a most promising pathway. Using last year's by-products, which are rich in nutrients and bioactive compounds, such as dietary fiber, polyphenols, and peptides, offer a wake-up call to the nutraceutical and cosmetic industry to invest and develop value-added products generated from food waste ingredients.

2.
Food Res Int ; 99(Pt 1): 328-335, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28784490

RESUMO

The aim was to study how factors such as temperature, alcoholic degree, and amino acids supplementation are able to influence the content of tyramine, histamine, 2-phenylethylamine, tryptamine and their precursor amino acids in winemaking process. Biogenic amines and amino acids were quantified at the beginning, middle and end of alcoholic fermentation, and at the end of malolactic fermentation. In general, samples produced with amino acid supplementation did not show the highest concentrations of biogenic amines, except for histamine, which content increased with the addition of the four amino acids. The synthesis of tyramine was mainly affected by the temperature and alcoholic degree, the formation of phenylethylamine was largely influenced by alcoholic degree, and tryptamine synthesis principally depended on temperature. Interestingly, there was interaction between these three factors for the biogenic amines studied. In conclusion, winemaking conditions should be established depending on the biogenic amine which synthesis is required to be controlled.


Assuntos
Aminoácidos/análise , Aminas Biogênicas/análise , Etanol/análise , Temperatura , Vinho/análise , Fermentação , Histamina/análise , Fenetilaminas/análise , Triptaminas/análise , Tiramina/análise
3.
Food Chem ; 197 Pt B: 1038-45, 2016 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-26675839

RESUMO

The validation of a HPLC-PDA-MS/MS chromatographic method for the quali/quantitative characterization of histaminol, hydroxytyrosol, tyrosol, and tryptophol in wine has been described and discussed. Four standards showed a good linearity with high correlation coefficient values (over 0.9989) and LOD and LOQ were 0.001-0.015 mg/L and 0.004-0.045 mg/L, respectively. Furthermore, this study reported how factors such as temperature, alcoholic degree, and amino acids concentration are able to influence the formation of these four alcohols in Monastrell wines. The quantification values of these alcohols has been detected both at the half and end of alcoholic fermentation, and at the end of malolactic fermentation. In relation to interactions between factors, several significant variations emerged (p ⩽ 0.001). The impact of amino acids supplementation in Monastrell must it has been demonstrated, mainly in regards to histaminol and tryptophol.


Assuntos
Fermentação , Histamina/análogos & derivados , Indóis/análise , Álcool Feniletílico/análogos & derivados , Vinho/análise , Aminoácidos/análise , Cromatografia Líquida de Alta Pressão , Histamina/análise , Histamina/biossíntese , Álcool Feniletílico/análise , Espectrometria de Massas em Tandem , Temperatura
4.
Artigo em Inglês | MEDLINE | ID: mdl-19760526

RESUMO

According to European Union Regulation EC 1531/2001, olive oil labelled as "extra-virgin" should be cold-pressed and contain no refined oil or oil from other oleaginous seeds or nuts. Adulteration of extra virgin olive oil (EVOO) with hazelnut oil (HAO) is a serious concern both for oil suppliers and consumers. The high degree of similarity between the two fats complicates the detection of low percentages of HAO in EVOO. Many analytical approaches have been developed in recent years to trace HAO in EVOO, principally based on chromatographic analyses, differential scanning calorimetry or nuclear magnetic resonance. In addition adulteration of EVOO with HAO may introduce hazelnut-derived allergens. The aim of this work was to analyse the protein and allergen content of EVOO intentionally spiked with raw cold-pressed HAO or solvent-extracted HAO. SDS-PAGE analysis confirmed the presence of hazelnut proteins in solvent-extracted HAO with molecular masses ranging 10-60 kDa. In contrast, cold-pressed HAO showed no traces of protein. In spiked EVOO, solvent-extracted HAO was still detectable at a 1% contamination level. Several bands on SDS-PAGE migrated at apparent molecular masses coinciding with known allergens, such as Cor a 1 (approximately 17 kDa), Cor a 2 (approximately 14 kDa), Cor a 8 (approximately 12 kDa), oleosin (approximately 17 kDa) and Cor a 9 (approximately 60 kDa). MALDI-TOF MS analysis confirmed the presence of two oleosin isoforms and of Cor a 9. Immunoblotting demonstrated that an allergic patient with known reactivity to Cor a 1 and Cor a 2 recognized a 17-kDa band in solvent-extracted HAO. In conclusion, we have shown that adulteration of extra virgin olive oil with solvent-extracted hazelnut oil can be traced by simple SDS-PAGE analysis, and that adulteration introduces a potential risk for hazelnut allergic patients.


Assuntos
Corylus/efeitos adversos , Corylus/imunologia , Gorduras Insaturadas na Dieta/efeitos adversos , Contaminação de Alimentos/análise , Hipersensibilidade Alimentar/etiologia , Óleos de Plantas/efeitos adversos , Alérgenos/análise , Alérgenos/genética , Sequência de Aminoácidos , Corylus/química , Corylus/genética , Gorduras Insaturadas na Dieta/análise , Eletroforese em Gel de Poliacrilamida , Hipersensibilidade Alimentar/imunologia , Humanos , Azeite de Oliva , Óleos de Plantas/análise , Proteínas de Plantas/efeitos adversos , Proteínas de Plantas/análise , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Fatores de Risco , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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