Comparison of annulus fibrosus cell collagen remodeling rates in a microtissue system.

It has been suggested that curvature progression in adolescent idiopathic scoliosis occurs through irreversible changes in the intervertebral discs. Strains of mice have been identified who differ in their disc wedging response upon extended asymmetrical compression. Annulus fibrosus (AF) tissue remodeling could contribute to the faster disc wedging progression previously observed in these mice. Differences in collagen remodeling capacity of AF cells between these in-bred mice strains were compared using an in vitro microtissue system. AF cells of 8-10-week-old LG/J ("fast-healing") and C57BL/6J ("normal healing") mice were embedded in a microtissue platform and cultured for 48 h. Hereafter, tissues were partially released and cultured for another 96 h. Microtissue surface area and waistcoat contraction, collagen orientation, and collagen content were measured. After 96 h postrelease, microtissues with AF cells of LG/J mice showed more surface area contraction (p < .001) and waistcoat contraction (p = .002) than C57BL/6J microtissues. Collagen orientation did not differ at 24 h after partial release. However, at 96 h, collagen in the microtissues from LG/J AF cells was aligned more than in those from C57BL/6J mice (p < .001). Collagen content did not differ between microtissues at 96 h. AF cells of inbred LG/J mice were better able to remodel and realign their collagen fibers than those from C57BL/6J mice. The remodeling of AF tissue could be contributing to the faster disc wedging progression observed in LG/J mice.

A comparison of growth among growth-friendly systems for scoliosis: a systematic review.

BACKGROUND CONTEXT: The optimal method for surgical treatment of early onset scoliosis is currently unknown. Although the aim of growth-friendly systems is to reduce the curve and maintain growth, there is no consensus on how to measure spinal growth during and after the treatment. Different measurements of different segments (T1-S1, T1-T12, instrumented length) are used for different time points to evaluate growth. The aim of this review is to assess what measurements are used and to compare the growth-friendly systems based on spinal growth during treatment. METHODS: The electronic MEDLINE, EMBASE, and Cochrane databases were systematically searched for original articles that reported growth for traditional growing rods (TGR), vertical expandable prosthetic titanium rib expansion technique (VEPTR), Shilla, magnetically controlled growing rods (MCGR), and Luque-trolley systems. All measurements were recorded, and weighted averages calculated in centimeter per year were compared. RESULTS: We included 52 studies (26 TGR, 12 MCGR, 6 VEPTR, 4 Luque trolley, 1 Shilla, and 3 mixed). Often only one segment was reported (T1-S1 length in 22 studies, T1-T12 length in two studies, and instrumented length in five studies). The remaining 22 studies reported T1-S1 length in combination with T1-T12 length (15 studies) or instrumented length (eight studies). Spinalgrowth achieved by initial correction only was a considerable 3.9 cm (based on 34 studies) as well as the spinal growth achieved by the final fusion surgery (2.3 cm in four studies). To specifically assess growth achieved with the system, length gain after initial surgery and before final fusion in growth system graduates was considered. Only four TGR studies reported on this "true" spinal growth with 0.6 and 0.3 cm/y in the T1-S1 and T1-T12 segment, respectively. CONCLUSIONS: Reporting on spinal growth is currently inadequate and does not allow a good comparison of different techniques. However, all systems often report growth similar to Dimeglio's T1-S1 spinal growth of 1 cm/y. It should be recognized though that a considerable portion of the reported spinal growth is the result of the initial and final surgical correction and not due to the growth-friendly implant.

A reliability and validity study for different coronal angles using ultrasound imaging in adolescent idiopathic scoliosis.

BACKGROUND CONTEXT: Radiation exposure remains a big concern in adolescent idiopathic scoliosis (AIS). Ultrasound imaging of the spine could significantly reduce or possibly even eliminate this radiation hazard. The spinous processes (SPs) and transverse processes (TPs) were used to measure the coronal deformity. Both landmarks provided reliable information on the severity of the curve as related to the traditional Cobb angle. However, it remained unclear which coronal ultrasound angle is the most appropriate method to measure the curve severity. PURPOSE: The objective of this study was to test the reliability and the validity of several ultrasound angle measurements in the coronal plane as compared with the radiographic coronal Cobb angle in patients with AIS. STUDY DESIGN/SETTING: This is a cross-sectional study. PATIENT SAMPLE: The study included 33 patients with AIS, both male and female (Cobb angle range: 3°-90°, primary and secondary curves), who underwent posterior-anterior radiography of the spine. OUTCOME MEASURES: The outcome measures were the reliability (intraclass correlation coefficients [ICCs] for the intra- and interobserver variabilities) and the validity (linear regression analysis and Bland-Altman method, including the mean absolute difference [MAD]) of different ultrasound measurements. MATERIALS AND METHODS: The patients were scanned using a dedicated ultrasound machine (Scolioscan, Telefield Medical Imaging Ltd, Hong Kong). The reliability and the validity were tested for three coronal ultrasound angles: an automatic and manual SP angle and a manual TP angle as compared with the radiographic coronal main thoracic or (thoraco)lumbar Cobb angles. RESULTS: The ICC showed very reliable measurements of all ultrasound methods (ICC ≥0.84). The ultrasound angles were 15%-37% smaller as compared with the Cobb angles; however, excellent linear correlations were seen between all ultrasound angles and the Cobb angle (thoracic: R2≥0.987 and (thoraco)lumbar R2≥0.970), and the Bland-Altman plot showed a good agreement between all ultrasound angles and the Cobb angle. The MADs of the ultrasound angles, corrected using the linear regression equation, and the Cobb angles showed no significant difference between the different ultrasound angles (MAD: automatic SP angle 4.9°±3.2°, manual SP angle 4.5°±3.1°, and manual TP angle 4.7°±3.6°; p≥.388). CONCLUSIONS: Coronal ultrasound angles are based on different landmarks than the traditional Cobb angle measurement and cannot represent the same angle values. In this study, we found excellent correlations between the ultrasound and Cobb measurements, without differences in the reliability and validity between the ultrasound angles based on the SPs and TPs. Therefore, the severity of the deformity in patients with AIS can be assessed by ultrasound imaging, avoiding hazardous ionizing radiation and enabling more individualized patient care. It also opens possibilities for screening.

Orthopaedic manifestations within the 22q11.2 Deletion syndrome: A systematic review.

The 22q11.2 Deletion Syndrome (22q11.2DS) is the most common microdeletion syndrome with an estimated prevalence of 1:4,000 live births. 22q11.2DS is known to have wide phenotypic variability, including orthopaedic manifestations. The purpose of this systematic review is to increase the awareness of orthopaedic manifestations associated with 22q11.2DS. This systematic review was performed according to the PRISMA Guidelines. Original epidemiological studies on the prevalence of orthopaedic manifestations within 22q11.2DS were systematically searched for in PubMed and EMBASE. The included articles were scored according to a risk-of-bias tool, a best-evidence synthesis was performed and the prevalence data was extracted. Sixty-nine published manuscripts described 58 orthopaedic manifestations in a total of 6,055 patients. The prevalence of at least one cervical or occipital anomaly is 90.5-100% (strong evidence). Fourteen studies (n = 2,264) revealed moderate evidence for a wide scoliosis prevalence of 0.6-60%. Two studies demonstrated that 5-6.4% of all 22q11.2DS patients required surgical scoliosis correction. Fifteen studies (n = 2,115) reported a 1.1-13.3% prevalence of clubfoot with moderate evidence. Other reported orthopaedic manifestations are patellar dislocation (10-20%), juvenile rheumatic arthritis (3.75%), impaired growth and skeletal anomalies like polydactyly (1.0-3.7%), syndactyly (11-11.8%), butterfly vertebrae (11.1%) and 13 ribs (2-19%). Orthopaedic findings are important manifestations of the 22q11.2DS, both in bringing patients to diagnostic attention and in requiring surveillance and appropriate intervention. Data on these manifestations are scattered and incomprehensive. Routinely screening for cervical anomalies, scoliosis, and upper and lower limb malformations is recommended in this vulnerable group of patients.