Administration of recombinant Reishi immunomodulatory protein (rLZ-8) diet enhances innate immune responses and elicits protection against nervous necrosis virus in grouper Epinephelus coioides.

Nervous necrosis virus (NNV) infection during larvae and juvenile stage in grouper (Epinephelus coioides) has caused severe economic losses in the aquaculture industry in Asia. The aims of this study were to evaluate the influence of recombinant Reishi protein, rLZ-8, on the innate immune responses and the viral resisting ability in fish. Groupers were fed with rLZ-8 supplemented diet (1.25-37.5 mg (rLZ-8)/kg(diet)), and the cytokine gene expression, innate immune responses, and survival rate after NNV challenge were examined. The fish fed with rLZ-8 diet showed 6- to 11-fold upregulated TNF-α and IL-1ß gene expression, along with significant increased respiratory burst and phagocytic activity. Moreover, feeding the fish with 37.5 mg/kg rLZ-8 diet elicited significant improvement in post viral challenge survival rate (85.7%). These discoveries indicated that rLZ-8 could be utilized as an ant-pathogen immunostimulant, and provided a new candidate to fight against NNV infection in fish.

Characteristics of a waveguide mode in a trilayer Ag/SiO(2)/Au plasmonic thermal emitter.

A suitably designed trilayer Ag/SiO(2)/Au thermal emitter can be used as the narrow bandwidth infrared light source. The thermal radiation generated in the SiO(2) layer resonates between the two metal films and results in not only the Ag/SiO(2) surface plasmon polaritons but also the waveguide mode (WM) in the Ag/SiO(2)/Au structure owing to the thick SiO(2) layer. This study investigated the influence of dielectric thickness on energy dispersion relations and derived the theoretical dispersion relation, which fit well with experimental results. This WM light source can be applied in the area of gas sensing and probing the response of the animal cells and plants to infrared radiation.

Effect of Wood's anomalies on the profile of extraordinary transmission spectra through metal periodic arrays of rectangular subwavelength holes with different aspect ratio.

The extraordinary transmission through silver film perforated with rectangular hole array with different aspect ratio was investigated. It was found that when the aspect ratio exceeded 7, the propagating surface plasmon polaritons (SPPs) transformed to localized resonance mode. The role of the Wood's anomaly on the shape of the transmission spectrum is investigated. By designing the rectangular hole arrays in a rectangular lattice, the Wood's anomaly can be shifted far apart from the transmission peak, the real localized resonance peak wavelength was identified and fitted well with the theoretical calculation using a simplified transmission-line model.

Mass transfer limit of fluorescent dyes during multicolor staining of aerobic granules.

Multicolor fluorescence experiments are conducted to investigate the distributions of extracellular polymeric substances and/or cells in the bioaggregates. Successful staining requires that the dyes could fully stain the targeted substances of bioaggregates in a finite time. The mass transfer limit for one of the four fluorescent dyes, calcofluor white, concanavalin A conjugated with tetramethylrhodamine, Nile red, and SYTO 63, penetrating entire phenol-fed granules or those sectioned at 50 microm thick, was quantitatively determined. The former three dyes sufficiently stained the entire granule within prescribed time intervals. However, the SYTO 63 could not penetrate the 600-microm granule in a finite time. Simplified one-dimensional diffusional model estimated the apparent diffusivity of SYTO 63 in the aerobic granule matrix. This work revealed that each staining scheme should be examined for the possible mass transfer limit of dyes during staining.

Reflection and emission properties of an infrared emitter.

The reflection and emission properties of an infrared emitter, which is a plasmonic multilayer structure consisting of a relief metallic grating, a waveguide layer, and a metallic substrate are investigated both experimentally and theoretically. A localized surface plasmon polariton (SPP) mode which is angular-independent in almost the full range of incident angles is observed. The thermal emission of this structure is also measured. It is found that the emission peak coincides with the angular-independent localized SPP mode. In addition, the emission spectrum of the plasmonic emitter can be predicted by investigating the reflectance spectrum.