A novel strain of lumpy skin disease virus causes clinical disease in cattle in Hong Kong.

Lumpy skin disease virus (LSDV) is an emerging poxviral pathogen of cattle that is currently spreading throughout Asia. The disease situation is of high importance for farmers and policy makers in Asia. In October 2020, feral cattle in Hong Kong developed multi-focal cutaneous nodules consistent with lumpy skin disease (LSD). Gross and histological pathology further supported the diagnosis and samples were sent to the OIE Reference Laboratory at The Pirbright Institute for confirmatory testing. LSDV was detected using quantitative polymerase chain reaction (qPCR) and additional molecular analyses. This is the first report of LSD in Hong Kong. Whole genome sequencing (WGS) of the strain LSDV/Hong Kong/2020 and phylogenetic analysis were carried out in order to identify connections to previous outbreaks of LSD, and better understand the drivers of LSDV emergence. Analysis of the 90 core poxvirus genes revealed LSDV/Hong Kong/2020 was a novel strain most closely related to the live-attenuated Neethling vaccine strains of LSDV and more distantly related to wildtype LSDV isolates from Africa, the Middle East and Europe. Analysis of the more variable regions located towards the termini of the poxvirus genome revealed genes in LSDV/Hong Kong/2020 with different patterns of grouping when compared to previously published wildtype and vaccine strains of LSDV. This work reveals that the LSD outbreak in Hong Kong in 2020 was caused by a different strain of LSDV than the LSD epidemic in the Middle East and Europe in 2015-2018. The use of WGS is highly recommended when investigating LSDV disease outbreaks.

Multifocal giant apocrine cystadenomas in a miniature poodle.

Apocrine cystadenomas have rarely been reported in dogs. Herein, a dog with multiple, large (1.0-7.0 cm) fluctuant and cystic skin masses on the right pelvic limb and chest is described. Histopathological evaluation revealed cystic cavities lined by single to multiple layers of apocrine epithelium with papillary projections, consistent with apocrine cystadenoma.

Les cystadénomes apocrines ont rarement été décrits chez le chien. Ici, un chien avec de multiples masses cutanées larges (1.0-7.0 cm), kystiques et fluctuantes sur le membre pelvien et le thorax, est décrit. L'évaluation histopathologique a montré des cavités kystiques entourées de couches simples à multiples d'épithélium apocrine avec projections papillaires, compatible avec un cystadénome apocrine.

Raramente se describen cistoadenomas apocrinos en perros. En este artículo se describe el caso de un perro con masas cutáneas múltiples, grandes (1,0 a 7,0 cm) fluctuantes y quísticas en la extremidad pélvica derecha y el pecho. La evaluación histopatológica reveló cavidades quísticas revestidas por capas individuales o múltiples de epitelio apocrino con proyecciones papilares, compatibles con cistoadenoma apocrino.

Os cistadenomas apócrinos já foram raramente descritos em cães. Descreve-se aqui o caso de um cão com múltiplas massas cutâneas grandes (1,0-7,0 cm), flutuantes e císticas no membro pélvico direito e no tórax. A avaliação histopatológica revelou cavidades císticas revestidas por uma a várias camadas de epitélio apócrino com projeções papilares, consistentes com cistadenoma apócrino.