[Determination of free d-biotin--comparison of instrumental and non-instrumental methods of analysis]. / Opredelenie svobodnogo d-biotina--sravnenie instumental'nogo i neinstrumental'nogo metodov analiza.

Simple methods for determining free d-biotin were developed based on the latex agglutination reaction inhibition and competitive ELISA. Biotin-binding protein Str used as a biospecific marker was immobilized on the surface of colored polyacrolein latex particles or a nitrocellulose membrane. The sensitivity of both methods of the free d-biotin assay, which take 1.5 h, is 1.0-3.0 ng/ml.