[The functional characteristics of the peripheral blood granulocytes in erysipelatous inflammation]. / Osobennosti sostoianiia granulotsitov perifericheskoi krovi pri rozhistom vospalenii.

The receptors (FcR, C3R) and functional activity, determined by the nitroblue tetrazolium (NRT) test, of polymorphonuclear leukocytes (PML) of low and normal density were studied in erysipelas patients. The leukocytes were obtained by sedimentation on the 2-stage gradient of Ficoll-Verographin (1.077 and 1.119 g/cu cm). No statistically significant difference in the average group indices between "light" and "normal" PNL of erysipelas patients were detected. In comparison with donor PNL, higher expression of C3R, a high spontaneous NBT(+)-PNL level and poor response to stimulation with IgG in the NBT test were observed on granulocytes of the patients. The short-term treatment of the whole blood obtained from the patients with Streptococcus haemolyticus allergen led to a significant increase in the output of "light" PNL. As negative control, brucellin treatment was used, which produced no essential effect. The treatment of donor blood with the above-mentioned antigens did not significantly affect the density of PNL. These facts suggest that in erysipelas the presence of "light" PNL is linked not with the release of granulocytes from the marrow, but with the activation of leukocytes by the products of infective inflammation.

[An assessment of body sensitization by the change in the density of the peripheral blood neutrophils]. / Otsenka sensibilizatsii organizma po izmeneniiu plotnosti neitrofilov perifericheskoi krovi.

A simple method for the assessment of the body sensitization has been developed, with peripheral blood neutrophils acting as the test system. The method consists in scintillation of the neutrophil count among the cells of the interphase ring obtained by centrifugation of 1:1 diluted peripheral blood in verografin density gradient, 1.077 g/cm3, after blood incubation with the tested allergen. Increase of the count of the neutrophils among other interphase cells by 20% or more after interaction with the allergen indicates a sensitization to this allergen. The accuracy and specificity of the method have been demonstrated in the examinations of patients with pollenosis and erysipelas.

[Functional activity of phagocytes in acute puerperal mastitis]. / Funktsional'naia aktivnost' fagotsitov pri ostrom poslerodovom mastite.

The authors examined 40 patients with acute puerperal mastitis and 34 healthy puerperas of the control group. Functional activity of the blood microphages and the breast milk macrophages according to the NST-test data has proved to be significantly increased, but at the same time, the decrease in potential possibilities of the cells was noted. Expression of both the types of the blood neutrophile granulocyte receptors increased, and that of the breast milk macrophages decreased.

[Immunobiological properties of staphylococcal enterotoxins type A, B, C, D and E]. / Immunobiologicheskie svoistva stafilokokkovykh énterotoksinov tipov A, B, C, D, E.

Comparative study of mitogenic and interferonogenic properties of staphylococcal enterotoxins of different serotypes is done. It is revealed that preparations of enterotoxins are polyclonal mitogens and have interferon-inducing activity. It is stated that enterotoxin of D type has the highest mitogenic activity, which is shown by interferon-inducing activity of A type toxin.

[Significance of the lability of expression of lymphocyte E-receptors in the informative value of the theophylline test in normal subjects and in patients with viral hepatitis B]. / Znachenie labil'nosti ékspressii E-retseptorov limfotsitov dlia informativnosti teofillinovogo testa v norme i pri virusnom gepatite B.

The informative value of the theophylline test has been estimated in normal subjects and in hepatitis B patients during the acute period of the disease. Experiments with varying length of incubation at 37 degrees C have revealed a lability of expression of the lymphocytic E-receptors in hepatitis B patients: this expression has been the maximum after 15 min incubation with theophylline and the E-RFC count reduced after 30 and 60 min incubation, with the E-RFC most sensitive to theophylline detectable after 15 and 30 min incubation. Correlation between the results of theophylline test and T gamma-cell count have shown identical results in normal subjects in all incubation terms when the maximum expression of E-receptors has been observed in the tested samples. In hepatitis B patients no coincidence of the results has been observed: the count of theophylline-sensitive E-RFC on conversion to the total E-RFC count surpassed the count of T gamma-cells. These results lead to a conclusion that the theophylline test should not be used for estimation of regulatory T-lymphocytes in the patients with viral hepatitis B.

[Evaluation of lymphocyte cooperative interaction with neutrophils by the stimulation of lymphokine production in erysipelatous inflammation]. / Otsenka kooperativnogo vzaimodeistviia limfotsitov s neitrofilami po stimuliatsii limfokinoobrazovaniia pri rozhistom vospalenii.

In erysipelatous inflammation lymphocytes were found to secrete lymphokine, both spontaneously and in response to in vitro stimulation with streptococcal allergen. The secreted lymphokine enhanced the oxygen-dependent metabolism of neutrophils. The lymphokine formation observed in this study depended on the clinical form of the disease. The character of the effect produced by lymphokine was determined by the initial state of target cells (macrophages).

[Characteristics of functional disorders of the dermal T-lymphocytes and macrophages in the basic clinical forms of erysipelas]. / Osobennosti narushenii funktsii T-limfotsitov i makrofagov dermy pri osnovnykh klinicheskikh formakh rozhi.

The "skin window" test with phytohemagglutinin and pyrogenal reveals the multiple character of disturbances in the cooperative interaction of dermal T-lymphocytes and macrophages in patients with clinical forms of erysipelas, which indicates the necessity of differentiated immunological correction.

[Effect of a streptococcal allergen on the rosette-forming capacity of the T-lymphocytes in erysipelas patients]. / Vliianie streptokokkovogo allergena na rozetkoobrazuiushchuiu sposobnost' T-limfotsitov bol'nykh rozhei.

The reaction of antigen-dependent E-rosette formation with the hemolytic streptococcal antigen in erysipelas patients is indicative of the ambiguous role of the specific immunological transformation of the body in respect of the infective agent antigens in different clinical forms of the disease and is of prognostic importance as regards the chronic transformation of the infectious process and the development of the relapses of the disease.

[Capacity of mononuclear cells to produce a factor that promotes E-rosette formation in different clinical forms of erysipelas]. / Sposobnost' mononuklearov vyrabatyvat' faktor, uvelichivaiushchii E-rozetkoobrazovanie, pri razlichnykh klinicheskikh formakh rozhi.

The author has examined the capacity of mononuclear cells in peripheral blood samples obtained from erysipelas patients for the in vitro secretion of E-rosette formation promoting factor (E-RPF) in response to polyclonal stimulation with phytohemagglutinin (PHA) P or antigenic stimulation with hemolytic streptococcal allergen. At the acute stage of the disease, mononuclear cells spontaneously secreted E-RPF in a half of the examined patients; at the same time, a statistically significant decrease in the PHA-induced secretion of E-RPF was observed, especially in patients with the primary bullous form and relapses of erysipelas. The optimum lymphokine production in response to PHA in patients with primary erysipelas was observed simultaneously with a low level of spontaneous lymphokine production, while in relapses it was significantly suppressed at all levels of the spontaneous secretion of E-RPF. The antigen-stimulated secretion of E-RPF was observed more frequently in patients with primary bullous erysipelas, while in bullous relapses it was completely absent. At the early stage of convalescence the intensity of the spontaneous secretion of E-RPF decreased, while the PHA-induced secretion of E-RPF enhanced. In relapses the secretion of E-RPF in response to stimulation with the specific allergen remained at a low level, while in convalescents having had primary erysipelas the level of this secretion was high. These data indicate that mononuclear cells in the peripheral blood essentially differ in their capacity to secrete E-RPF in response to polyclonal and antigenic stimulation in various clinical forms of erysipelas.

[Effect of levamisole on the B immunity system indices of erysipelas patients]. / Vliianie levamizola na pokazateli B-sistemy immuniteta u bol'nykh rozhei.

When used for the treatment of patients with different clinical forms of erysipelas, levamisole induced a significant decrease in the B-lymphocyte level in the peripheral blood and the concentration of IgG and IgA, previously elevated.