Wilson disease with thrombocytopenia (case report).

We present an adolescent patient with WD accompanied with secondary amenorrhea, and thrombocytopenia. NK, a 14 year-old girl, had amenorrhea for 5 months despite having had regular menses for 2 years. An abdominal ultrasound scan revealed ascitis and some ovarian cysts. On physical examination: slight jaundice, edema of lower extremities, skin purpuric rash, enlarged abdomen, dry skin. She had no hepatomegaly and no splenomegaly. Breast and pubic hair development was concomitant with Tanner stage 4. There was performed laboratory and instrumental investigations. The patient was diagnosed as WD owing to the low level of ceruloplasmin, with increased level of copper in 24-hour urine excretion and in dry liver tissue. The needle biopsy of liver showed severe hepatocellular necrosis, inflammatory changes and fibrosis. The platelet count was found to be low with lack of increased number of megakaryocytes in the bone marrow aspiration suggesting the thrombocytopenia was not exclusively owing to hypersplenism. The absence of antithrombocyte and other autoimmune and viral antibodies excluded respectively the diagnosis of autoimmune thrombocytopenia, other autoimmune diseases and viral infections. Thus, we support the recommendation that adolescents with amenorrhea or children with thrombocytopenia without any obvious cause should be evaluated for WD, because the early detection and treatment of WD is capable of reversing described changes and restoring a normal liver function.

The intermittent and prolonged maternal separation affects behavioral reactions in rat pups.

The work was designed to reveal the effect of maternal deprivation on the behavioral reactions of rat pups during the intermittent and prolonged maternal separation. Research was conducted on 126 white rat pups of both sexes received from 26 mothers at the 1st, 15th, 21st, and 60th days after birth. All animals were divided into three main groups: the first group included 42 litters submitted to intermittent maternal deprivation (IMD). The second group included 28 litters submitted to prolonged maternal deprivation (PMD) and 56 litters served as the control group. Animals were housed in a room with a controlled temperature, humidity, 12 h light/dark cycle, having free access to the food and water. The received results testify to essential influence of maternal deprivation on the normal functioning of CNS, revealed in the alterations of behaviors in rats suffering from maternal deprivation. Thus, early maternal separation in rat pups may serve to be the stressful stimulus for CNS and alter it function, which produces the behavioural disorders among them.

Parameters of oxidative metabolism in neonates suffering from sepsis and anemia.

Neonatal sepsis still remains as one of the actual problems in modern medicine due to its high morbidity and mortality rates determined by diagnostic difficulties and absence of sufficient evidence for effective therapy. Literature data have shown that essential role in pathogenesis of sepsis belongs to the cellular oxidation-reduction misballance and development of the oxidative stress. The aim of our work was to assess indices of pro- and antioxidant systems in term neonates with sepsis on the background of anemia and without it. A total of 41 neonates (17 male, 24 female) with the age range from 3 to 7 days, with early sepsis, and in 2003-2005 years treated at the department of neonates' therapy and intensive care unit of pediatric clinics of the Tbilisi State Medical University were under observation. The control group involved 17 practically healthy neonates of the same age range. In consequence of the analyses there was ascertained, that with anemia increases intensification free-radical oxidation process. At the same time, antioxidant system activity was not change significantly in the sepsis with anemia, than other one. Pathogenesis of anemia may was founded undergo hemolitic anemia results by oxidative stress. According to the results of investigations could be concluded that in case of anemia developed at neonatal sepsis supports intensify of oxidative stress and at the same time anemia is the result of the oxidative stress.

[Oxidative metabolism parameters in newborns with sepsis during the cerebral ischemia of different degrees].

Within the last decade neonatal sepsis remains one of the actual problems of medicine due to its high morbidity and lethality rates. Reference data clarify that in septicaemia pathogenesis a great importance is attributed to the cellular oxidation-reduction imbalance and oxide stress development. A redox imbalance developed during sepsis indicates to the cell's dysfunction. Also central nervous system is one of the first organs, which is damaged during the sepsis. The purpose of our research was the investigation of activity of pro- and antioxidant system in newborn babies with sepsis during the cerebral ischemia of different degrees. It was shown that during the newborns' sepsis the intensity of free-radical oxidation-reduction process was increased remarkably, antioxidant system activity decreased and intensive ESR-signal of HbNO MetHb was fixed in the blood. These changes caused to development of hypoxemia and deep cerebral ischemia.