Bacterial cellulose matrix with in situ impregnation of silver nanoparticles via catecholic redox chemistry for third degree burn wound healing.

In the present study, bacterial cellulose (BC) based nanocomposite dressing material was developed for third burn wound management by polydopamine (PD) coated BC with in situ reduction of silver nanoparticles (BC-PDAg). BC-PDAg nanocomposite was characterized to understand the morphological, physical and chemical properties. Antimicrobial activity of BC-PDAg against burn wound specific pathogens were significant. The in vitro cytotoxicity and proliferation studies revealed that BC-PDAg nanocomposite is biocompatible and it supports cell proliferation. Further, in vivo experiments on female albino Wistar rats confirmed that BC-PDAg was effective in wound healing by promoting re-epithelization, and collagen deposition as evidenced by histopathological analysis. Moreover, molecular gene expression study has revealed that BC-PDAg promotes healing process by regulating the expression of inflammatory, angiogenesis and growth factor genes. The overall performance of BC-PDAg nanocomposite suggests that it could be used as promising skin regenerative tool in modern medicine.

Thymol enriched bacterial cellulose hydrogel as effective material for third degree burn wound repair.

Bacterial cellulose is well known for its excellent contributions in biomedical applications due to its superior properties. However the lack of antimicrobial property restricts its use in wound healing. To address the complications in third degree burns, thymol enriched bacterial cellulose hydrogel (BCT) was developed in this study. The incorporation of thymol into bacterial cellulose along with its chemical and thermal changes were investigated by FTIR, TGA and DSC respectively. Antimicrobial studies revealed that BCT possess excellent biocidal activity against burn specific pathogens. The in vitro biocompatibility studies were carried out in mouse 3T3 fibroblast cells. The BCT hydrogel facilitated the growth of fibroblast cells, exhibiting low toxicity, and increased cell viability. The burn wound healing efficiency of the BCT hydrogel was examined in vivo using female albino Wistar rats. Histopathological studies reveal that the wound treated with BCT hydrogel showed faster wound closure than BC and control groups. All these findings, suggest that BCT hydrogel can be used as resourceful and natural burn wound dressing material.

Novel fibrous collagen-based cream accelerates fibroblast growth for wound healing applications: in vitro and in vivo evaluation.

The present study reports the development of a novel film-forming bovine collagenous cream (BCC) based on bovine collagen (BC). In this study, collagen was isolated from bovine forestomach tissue, a novel source, and a cream formulation was prepared using some other bioactive ingredients. The electrophoretic pattern of the BC was found to be similar to type I collagen. The purity of BC was examined by amino acid analysis, which confirmed the presence of atelocollagen. The physicochemical properties of BCC such as rheology, spreadability, and temperature stability were characterized. The antimicrobial activity was examined against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli, and BCC displayed excellent inhibitory effect. In vitro biocompatibility studies using NIH 3T3 fibroblast cells showed enhanced cell viability. FACS analysis revealed the non-toxic nature of BCC toward cells. The cell morphology and proliferation on the BCC matrix was studied using SEM and fluorescence microscopy. The in vivo wound healing efficacy of the BCC as a topical wound dressing was demonstrated on full thickness excision wounds in rat models. The healing profile showed that the BCC significantly enhanced re-epithelialization, collagen deposition, and contraction in the wound healing process. The findings of this study provide a new opportunity for the utilization of the untapped byproducts of the meat industry for valorization. We expect that this kind of topical healing cream could be a potential candidate in wound management and future clinical needs.