RESUMO
The accumulation of red ketocarotenoids is an important component of coloration in many organisms, but the underlying mechanisms are poorly understood. In some organisms, ketocarotenoids are sequestered from the diet and can accumulate when enzymes responsible for carotenoid breakdown are disrupted. In other organisms, ketocarotenoids are formed endogenously from dietary precursors via oxidation reactions carried out by carotenoid ketolase enzymes. Here, we study the genetic basis of carotenoid coloration in an amphibian. We demonstrate that a red/yellow polymorphism in the dendrobatid poison frog Ranitomeya sirensis is due to the presence/absence of ketocarotenoids. Using whole-transcriptome sequencing of skins and livers, we found that a transcript encoding a cytochrome P450 enzyme (CYP3A80) is expressed 3.4-fold higher in livers of red frogs versus yellow. As CYP3A enzymes are known carotenoid ketolases in other organisms, our results point to CYP3A80 as a strong candidate for a carotenoid ketolase in amphibians. Furthermore, in red frogs, the transcript encoding the carotenoid cleavage enzyme BCO2 is expressed at a low level or as a splice variant lacking key catalytic amino acids. This suggests that BCO2 function may be disrupted in red frogs, providing a mechanism whereby the accumulation of ketocarotenoids and their dietary precursors may be enhanced.
Assuntos
Anuros/genética , Carotenoides , Pigmentação , Animais , Sistema Enzimático do Citocromo P-450/genética , Dieta/veterinária , Fígado/enzimologia , Pigmentação/genéticaRESUMO
In animals, bright colors often evolve to mimic other species when a resemblance is selectively favored. Understanding the proximate mechanisms underlying such color mimicry can give insights into how mimicry evolves-for example, whether color convergence evolves from a shared set of mechanisms or through the evolution of novel color production mechanisms. We studied color production mechanisms in poison frogs (Dendrobatidae), focusing on the mimicry complex of Ranitomeya imitator. Using reflectance spectrometry, skin pigment analysis, electron microscopy, and color modeling, we found that the bright colors of these frogs, both within and outside the mimicry complex, are largely structural and produced by iridophores but that color production depends crucially on interactions with pigments. Color variation and mimicry are regulated predominantly by iridophore platelet thickness and, to a lesser extent, concentration of the red pteridine pigment drosopterin. Compared with each of the four morphs of model species that it resembles, R. imitator displays greater variation in both structural and pigmentary mechanisms, which may have facilitated phenotypic divergence in this species. Analyses of nonmimetic dendrobatids in other genera demonstrate that these mechanisms are widespread within the family and that poison frogs share a complex physiological "color palette" that can produce diverse and highly reflective colors.
Assuntos
Anuros/fisiologia , Evolução Biológica , Pigmentação , Pigmentos Biológicos/fisiologia , Animais , Cor , Microscopia Eletrônica de Transmissão/veterinária , Pele/ultraestrutura , Pigmentação da Pele/fisiologiaRESUMO
Chemical signaling in animals often plays a central role in eliciting a variety of responses during reproductive interactions between males and females. One of the best-known vertebrate courtship pheromone systems is sodefrin precursor-like factors (SPFs), a family of two-domain three-finger proteins with a female-receptivity enhancing function, currently only known from salamanders. The oldest divergence between active components in a single salamander species dates back to the Late Paleozoic, indicating that these proteins potentially gained a pheromone function earlier in amphibian evolution. Here, we combined whole transcriptome sequencing, proteomics, histology, and molecular phylogenetics in a comparative approach to investigate SPF occurrence in male breeding glands across the evolutionary tree of anurans (frogs and toads). Our study shows that multiple families of both terrestrially and aquatically reproducing frogs have substantially increased expression levels of SPFs in male breeding glands. This suggests that multiple anuran lineages make use of SPFs to complement acoustic and visual sexual signaling during courtship. Comparative analyses show that anurans independently recruited these proteins each time the gland location on the male's body allowed efficient transmission of the secretion to the female's nares.
Assuntos
Anuros/metabolismo , Atrativos Sexuais/metabolismo , Animais , Anuros/genética , Glândulas Exócrinas/metabolismo , Feminino , Masculino , Oligopeptídeos/metabolismo , Filogenia , Caracteres Sexuais , Sequenciamento do ExomaRESUMO
Animal sex pheromone systems often exist as multicomponent signals [1-11] to which chemical cues have been added over evolutionary time. Little is known on why and how additional molecules become recruited and conserved in an already functional pheromone system. Here, we investigated the evolutionary trajectory of a series of 15 kDa proteins-termed persuasins-that were co-opted more recently alongside the ancient sodefrin precursor-like factor (SPF) courtship pheromone system in salamanders [9, 12]. Expression, genomic, and molecular phylogenetic analyses show that persuasins originated from a gene that is expressed as a multi-domain protein in internal organs where it has no pheromone function but underwent gene duplication and neofunctionalization. The subsequent evolution combined domain loss and the introduction of a proteolytic cleavage site in the duplicated gene to give rise to two-domain cysteine rich proteins with structural properties similar to SPF pheromones [12]. An expression shift to the pheromone-producing glands, where expression of persuasins was immediately spatiotemporally synchronized with the already available pheromone system, completed the birth of a new pheromone. Electrostatic forces between members of both protein families likely enhance co-localization and simultaneous activation of different female olfactory neurons, explaining why persuasins immediately had a selective advantage. In line with this, behavioral assays show that persuasins increase female receptivity on their own but also exert a cumulative or synergistic effect in combination with SPF, clearly reinforcing the pheromone system as a whole. Our study reveals molecular remodeling of an existing protein architecture as an evolutionary mechanism for functional reinforcement of animal pheromone systems.
Assuntos
Adaptação Biológica , Proteínas de Anfíbios/genética , Atrativos Sexuais/fisiologia , Urodelos/fisiologia , Sequência de Aminoácidos , Proteínas de Anfíbios/química , Proteínas de Anfíbios/metabolismo , Animais , Evolução Molecular , Feminino , Masculino , Filogenia , Alinhamento de Sequência , Atrativos Sexuais/química , Atrativos Sexuais/genética , Especificidade da Espécie , Urodelos/genéticaRESUMO
In animals that use chemical communication during courtship and reproduction, speciation is often associated with divergence of their sex pheromones. In multicomponent pheromone systems, divergence can be obtained either by adding or deleting components, or by altering the relative contribution of individual components to the mixture. Protein pheromone systems can additionally evolve by amino acid sequence divergence to produce pheromones with a species-specific effect. The sodefrin precursor-like factor (SPF) pheromone system, a blend of proteins that essentially enhances receptivity in salamanders, has had a long and dynamic evolution of gene duplications, but the mechanisms that govern interspecific divergence and the role they play in reproductive isolation remain elusive. Here, we use transcriptomics and proteomics to characterize the SPF protein repertoire of the alpine newt (Ichthyosaura alpestris), and compare it to the previously identified repertoire of SPF proteins of the palmate newt (Lissotriton helveticus), a related but nonhybridizing species. Subsequent phylogenetic analyses indicate that, despite the availability of multiple SPF gene copies, both species predominantly express the same subset of orthologs. Our study demonstrates that species specificity in the SPF protein pheromone system can be established by gradual sequence divergence of the same set of proteins alone.
Assuntos
Variação Genética/genética , Filogenia , Salamandridae/genética , Atrativos Sexuais/genética , Animais , Evolução Molecular , Feminino , Masculino , Proteínas/genética , Salamandridae/fisiologia , Comportamento Sexual Animal , Especificidade da EspécieRESUMO
Sodefrin, a decapeptide isolated from the male dorsal gland of the Japanese fire belly newt Cynops pyrrhogaster, was the first peptide pheromone identified from a vertebrate. The fire belly salamander and sodefrin have become a model for sex pheromone investigation in aquatically courting salamanders ever since. Subsequent studies in other salamanders identified SPF protein courtship pheromones of around 20 kDa belonging to the same gene-family. Although transcripts of these proteins could be PCR-amplified in Cynops, it is currently unknown whether they effectively use full-length SPF pheromones next to sodefrin. Here we combined transcriptomics, proteomics and phylogenetics to investigate SPF pheromone use in Cynops pyrrhogaster. Our data show that not sodefrin transcripts, but multiple SPF transcripts make up the majority of the expression profile in the dorsal gland of this newt. Proteome analyses of water in which a male has been courting confirm that this protein blend is effectively secreted and tail-fanned to the female. By combining phylogenetics and expression data, we show that independent evolutionary lineages of these SPF's were already expressed in ancestral Cynops species before the origin of sodefrin. Extant Cynops species continue to use this multi-component pheromone system, consisting of various proteins in addition to a lineage-specific peptide.
Assuntos
Oligopeptídeos/metabolismo , Feromônios/metabolismo , Salamandridae/metabolismo , Animais , Feminino , Masculino , Especificidade da EspécieRESUMO
Sex pheromones have been shown to constitute a crucial aspect of salamander reproduction. Until now, courtship pheromones of Salamandridae and Plethodontidae have been intensively studied, but information on chemical communication in other urodelan families is essentially lacking. The axolotl (Ambystoma mexicanum, Ambystomatidae) has a courtship display that suggests a key role for chemical communication in the orchestration of its sexual behavior, but no sex pheromones have yet been characterized from this species. Here we combined whole transcriptome analyses of the male cloaca with proteomic analyses of water in which axolotls were allowed to court to show that male axolotls secrete multiple ca. 20 kDa glycosylated sodefrin precursor-like factor (SPF) proteins during courtship. In combination with phylogenetic analyses, our data show that the male cloaca essentially secretes a courtship-specific clade of SPF proteins that is orthologous to salamandrid courtship pheromones. In addition, we identified an SPF protein for which no orthologs have been described from other salamanders so far. Overall, our study advocates a central role for SPF proteins during the courtship display of axolotls and adds knowledge on pheromone use in a previously unexplored deep evolutionary branch of salamander evolution.
Assuntos
Ambystoma mexicanum/metabolismo , Feromônios/metabolismo , Sequência de Aminoácidos , Animais , Teorema de Bayes , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Cloaca/metabolismo , Corte , Feminino , Perfilação da Expressão Gênica , Masculino , Espectrometria de Massas , Modelos Animais , Dados de Sequência Molecular , Feromônios/análise , Feromônios/classificação , Filogenia , Proteômica , Alinhamento de Sequência , Comportamento Sexual AnimalRESUMO
Despite renewed interest in the biogeography and evolutionary history of Old World tree frogs (Rhacophoridae), this family still includes enigmatic frogs with ambiguous phylogenetic placement. During fieldwork in four northeastern states of India, we discovered several populations of tree hole breeding frogs with oophagous tadpoles. We used molecular data, consisting of two nuclear and three mitochondrial gene fragments for all known rhacophorid genera, to investigate the phylogenetic position of these new frogs. Our analyses identify a previously overlooked, yet distinct evolutionary lineage of frogs that warrants recognition as a new genus and is here described as Frankixalus gen. nov. This genus, which contains the enigmatic 'Polypedates' jerdonii described by Günther in 1876, forms the sister group of a clade containing Kurixalus, Pseudophilautus, Raorchestes, Mercurana and Beddomixalus. The distinctiveness of this evolutionary lineage is also corroborated by the external morphology of adults and tadpoles, adult osteology, breeding ecology, and life history features.
Assuntos
Estruturas Animais/anatomia & histologia , Anuros/classificação , Anuros/genética , Animais , Anuros/anatomia & histologia , DNA/análise , Evolução Molecular , Larva/anatomia & histologia , FilogeniaRESUMO
BACKGROUND: Male salamanders (Urodela) often make use of pheromones that are produced in sexually dimorphic glands to persuade the female into courtship and mating. The mental gland of lungless salamanders (Plethodontidae) and dorsal cloacal glands (or abdominal glands) of newts (Salamandridae) have been particularly well studied in that respect. In both families, sodefrin precursor-like factor (SPF) proteins have been identified as major components of the courtship pheromone system. However, similar to plethodontids, some newts also make use of subtle head glands during courtship, but few pheromones have been characterized from such structures. Males of red-spotted newts (Notophthalmus viridescens, Salamandridae) have both cloacal and cheek (genial) glands, and are known to apply secretions to the female's nose by both tail-fanning and cheek-rubbing. Here we combined transcriptomic and phylogenetic analyses to investigate the presence, diversity and evolution of SPF proteins in the cloacal and cheek glands of this species. RESULTS: Our analyses indicate that the cheek glands of male N. viridescens produce a similar amount and diversity of SPF isoforms as the cloacal glands in this species. Expression in other tissues was much lower, suggesting that both male-specific courtship glands secrete SPF pheromones during courtship. Our phylogenetic analyses show that N. viridescens expresses a combination of isoforms that stem from four highly diverged evolutionary lineages of SPF variants, that together form a basis for the broad diversity of SPF precursors in the breeding glands. CONCLUSIONS: The similar SPF expression of cheek and cloacal glands suggests that this protein family is used for pheromone signalling through cheek rubbing in the red-spotted newt. Since several male salamandrids in other genera have comparable head glands, SPF application via other glands than the cloacal glands may be more widespread than currently appreciated in salamandrids.
Assuntos
Notophthalmus viridescens/fisiologia , Animais , Corte , Glândulas Exócrinas/química , Feminino , Masculino , Oligopeptídeos/química , Oligopeptídeos/genética , Oligopeptídeos/metabolismo , Feromônios/química , Feromônios/genética , Feromônios/metabolismo , Filogenia , Proteínas/genéticaRESUMO
Males of the advanced salamanders (Salamandroidea) attain internal fertilization without a copulatory organ by depositing a spermatophore on the substrate in the environment, which females subsequently take up with their cloaca. The aquatically reproducing modern Eurasian newts (Salamandridae) have taken this to extremes, because most species do not display close physical contact during courtship, but instead largely rely on females following the male track at spermatophore deposition. Although pheromones have been widely assumed to represent an important aspect of male courtship, molecules able to induce the female following behaviour that is the prelude for successful insemination have not yet been identified. Here, we show that uncleaved sodefrin precursor-like factor (SPF) protein pheromones are sufficient to elicit such behaviour in female palmate newts (Lissotriton helveticus). Combined transcriptomic and proteomic evidence shows that males simultaneously tail-fan multiple ca 20 kDa glycosylated SPF proteins during courtship. Notably, molecular dating estimates show that the diversification of these proteins already started in the late Palaeozoic, about 300 million years ago. Our study thus not only extends the use of uncleaved SPF proteins outside terrestrially reproducing plethodontid salamanders, but also reveals one of the oldest vertebrate pheromone systems.
Assuntos
Proteínas de Anfíbios/metabolismo , Atrativos Sexuais/metabolismo , Urodelos/fisiologia , Sequência de Aminoácidos , Proteínas de Anfíbios/genética , Animais , Sequência de Bases , Corte , Feminino , Masculino , Dados de Sequência Molecular , Filogenia , Proteoma , Atrativos Sexuais/genética , Comportamento Sexual Animal , Especificidade da Espécie , Transcriptoma , Urodelos/genéticaRESUMO
Sex pheromones form an important facet of reproductive strategies in many organisms throughout the Animal Kingdom. One of the oldest known sex pheromones in vertebrates are proteins of the Sodefrin Precursor-like Factor (SPF) system, which already had a courtship function in early salamanders. The subsequent evolution of salamanders is characterized by a diversification in courtship and reproduction, but little is known on how the SPF pheromone system diversified in relation to changing courtship strategies. Here, we combined transcriptomic, genomic, and phylogenetic analyses to investigate the evolution of the SPF pheromone system in nine salamandrid species with distinct courtship displays. First, we show that SPF originated from vertebrate three-finger proteins and diversified through multiple gene duplications in salamanders, while remaining a single copy in frogs. Next, we demonstrate that tail-fanning newts have retained a high phylogenetic diversity of SPFs, whereas loss of tail-fanning has been associated with a reduced importance or loss of SPF expression in the cloacal region. Finally, we show that the attractant decapeptide sodefrin is cleaved from larger SPF precursors that originated by a 62 bp insertion and consequent frameshift in an ancestral Cynops lineage. This led to the birth of a new decapeptide that rapidly evolved a pheromone function independently from uncleaved proteins.
Assuntos
Atrativos Sexuais/genética , Urodelos/genética , Urodelos/metabolismo , Animais , Evolução Molecular , Atrativos Sexuais/classificaçãoRESUMO
Males of many frog species develop spiny nuptial pads with underlying glands on their thumbs during the mating period. We used 3D visualization on the European common frog Rana temporaria to show that the morphology of these glands allows the channelling of secreted molecules to the pad's surface during amplexus. Combined transcriptome and proteome analyses show that proteins of the Ly-6/uPAR family, here termed amplexins, are highly expressed in the nuptial glands during the mating season, but are totally absent outside that period. The function of amplexins remains unknown, but it is interesting to note that they share structural similarities with plethodontid modulating factors, proteins that influence courtship duration in salamanders.
Assuntos
Comunicação Animal , Anuros/fisiologia , Membro Anterior/metabolismo , Atrativos Sexuais/metabolismo , Caracteres Sexuais , Animais , Anuros/metabolismo , Cromatografia Líquida de Alta Pressão , Biblioteca Gênica , Técnicas Histológicas , Espectrometria de Massas , Reação em Cadeia da Polimerase , Atrativos Sexuais/genética , Especificidade da Espécie , Urodelos/metabolismo , Microtomografia por Raio-XRESUMO
Amphibians have invaded arboreal habitats multiple times independently during their evolution. Adaptation to these habitats was nearly always accompanied by the presence or appearance of toe pads, flattened enlargements on tips of fingers and toes that provide adhesive power in these environments. The strength and elasticity of the toe pad relies on polygonal arrayed cells ending in nanoscale projections, which are densely packed with cytoskeletal proteins. Here, we characterized and determined the evolutionary origin of these proteins in the toe pad of the tree frog Hyla cinerea. We created a subtracted cDNA library enriching genes that are expressed in the toe pad, but nowhere else in the toe. Our analyses revealed five alpha keratins as main structural proteins of the amphibian toe pad. Phylogenetic analyses show that these proteins belong to different keratin lineages that originated in an early tetrapod ancestor and in mammals evolved to become the major keratin types of hair. The ancestral keratins were probably already expressed in areas that required skin reinforcement in early tetrapods, and subsequently diverged to support fundamentally different adaptive structures in amphibians and mammals.
Assuntos
Anfíbios/fisiologia , Pé/fisiologia , Cabelo/fisiologia , Queratinas/metabolismo , Mamíferos/fisiologia , Animais , Cabelo/metabolismo , RecidivaRESUMO
Internal fertilization without copulation or prolonged physical contact is a rare reproductive mode among vertebrates. In many newts (Salamandridae), the male deposits a spermatophore on the substrate in the water, which the female subsequently takes up with her cloaca. Because such an insemination requires intense coordination of both sexes, male newts have evolved a courtship display, essentially consisting of sending pheromones under water by tail-fanning towards their potential partner. Behavioral experiments until now mostly focused on an attractant function, i.e. showing that olfactory cues are able to bring both sexes together. However, since males start their display only after an initial contact phase, courtship pheromones are expected to have an alternative function. Here we developed a series of intraspecific and interspecific two-female experiments with alpine newt (Ichthyosaura alpestris) and palmate newt (Lissotriton helveticus) females, comparing behavior in male courtship water and control water. We show that male olfactory cues emitted during tail-fanning are pheromones that can induce all typical features of natural female mating behavior. Interestingly, females exposed to male pheromones of their own species show indiscriminate mating responses to conspecific and heterospecific females, indicating that visual cues are subordinate to olfactory cues during courtship.
Assuntos
Corte , Amor , Atrativos Sexuais/farmacologia , Comportamento Sexual Animal/efeitos dos fármacos , Animais , Cloaca/efeitos dos fármacos , Sinais (Psicologia) , Feminino , Inseminação/efeitos dos fármacos , Masculino , Percepção Olfatória/efeitos dos fármacos , Salamandridae , Comportamento Sexual Animal/fisiologia , Especificidade da Espécie , Espermatogônias/citologiaAssuntos
Altitude , Anuros/genética , Variação Genética , Animais , Fluxo Gênico , Modelos Lineares , Lagartos/genética , América do SulRESUMO
The limbless, primarily soil-dwelling and tropical caecilian amphibians (Gymnophiona) comprise the least known order of tetrapods. On the basis of unprecedented extensive fieldwork, we report the discovery of a previously overlooked, ancient lineage and radiation of caecilians from threatened habitats in the underexplored states of northeast India. Molecular phylogenetic analyses of mitogenomic and nuclear DNA sequences, and comparative cranial anatomy indicate an unexpected sister-group relationship with the exclusively African family Herpelidae. Relaxed molecular clock analyses indicate that these lineages diverged in the Early Cretaceous, about 140 Ma. The discovery adds a major branch to the amphibian tree of life and sheds light on both the evolution and biogeography of caecilians and the biotic history of northeast India-an area generally interpreted as a gateway between biodiversity hotspots rather than a distinct biogeographic unit with its own ancient endemics. Because of its distinctive morphology, inferred age and phylogenetic relationships, we recognize the newly discovered caecilian radiation as a new family of modern amphibians.
Assuntos
Anfíbios/classificação , Anfíbios/genética , Demografia , Evolução Molecular , Variação Genética , Filogenia , Crânio/anatomia & histologia , África , Anfíbios/anatomia & histologia , Animais , Sequência de Bases , Teorema de Bayes , DNA Mitocondrial/genética , Espécies em Perigo de Extinção , Haplótipos/genética , Índia , Modelos Genéticos , Dados de Sequência Molecular , Filogeografia , Análise de Sequência de DNA , Tomografia Computadorizada por Raios XRESUMO
Night frogs (Nyctibatrachidae) form a family endemic to the Western Ghats, a hill chain along the west coast of southern India. Extant members of this family are descendants of a lineage that originated on the subcontinent during its longtime isolation in the Late Cretaceous. Because the evolutionary history of Nyctibatrachidae has always been tightly connected to the subcontinent, these tropically-adapted frogs are an ideal group for studying how patterns of endemism originated and evolved during the Cenozoic in the Western Ghats. We used a combined set of mitochondrial and nuclear DNA fragments to investigate the phylogenetic relationships of 120 ingroup specimens of all known species of Nyctibatrachidae. Our analyses indicate that, although this family had an early origin on the Indian subcontinent, the early diversification of extant nyctibatrachids happened only in the Eocene. Biogeographic analyses show that dispersal across the Palghat gap and Shencottah gap was limited, which led to clade endemism within mountain ranges of the Western Ghats. It is likely that multiple biota have been affected simultaneously by these prominent geographical barriers. Our study therefore further highlights the importance of considering the Western Ghats-Sri Lanka biodiversity hotspot as an assemblage of distinct mountain regions, each containing endemism and deserving attention in future conservation planning.
Assuntos
Ranidae/classificação , Ranidae/genética , Animais , Biodiversidade , Evolução Biológica , DNA Mitocondrial/química , Genes de RNAr , Índia , Dados de Sequência Molecular , Filogenia , FilogeografiaRESUMO
Recent studies have identified range expansion as a potential driver of speciation. Yet it remains poorly understood how, under identical extrinsic settings, differential tendencies for geographic movement of taxa originate and subsequently affect diversification. We identified multiple traits that predict large distributional ranges in extant species of toads (Bufonidae) and used statistical methods to define and phylogenetically reconstruct an optimal range-expansion phenotype. Our results indicate that lineage-specific range-shifting abilities increased through an accumulation of adaptive traits that culminated in such a phenotype. This initiated the episode of global colonization and triggered the major radiation of toads. Evolution toward a range-expansion phenotype might be crucial to understanding both ancient widespread radiations and the evolutionary background of contemporary invasive species such as the cane toad.
Assuntos
Adaptação Biológica , Evolução Biológica , Bufonidae , África , Animais , Ásia , Austrália , Teorema de Bayes , Bufonidae/anatomia & histologia , Bufonidae/classificação , Bufonidae/genética , Bufonidae/fisiologia , Especiação Genética , Geografia , Funções Verossimilhança , Dados de Sequência Molecular , Fenótipo , Filogenia , Dinâmica Populacional , Reprodução , América do Sul , Especificidade da EspécieRESUMO
BACKGROUND: Bufonidae are a large family of toads with a subcosmopolitan distribution. Recent molecular phylogenetic analyses have revealed a radiation of toads (Adenominae) with distinct adult and larval ecomorphs on the Southern parts of the Indian subcontinent. The Indian torrential species "Ansonia" ornata has a basal position in this clade and does not group with South-East Asian Ansonia. Additionally, the nested position of "Bufo" koynayensis and an undescribed sister species, and their distinct ecologies including a non-typical egg-laying strategy within bufonids, support the recognition of a second distinct genus. In this paper we describe two new genera and one new species from the Adenominae clade. FINDINGS: Ansonia ornata Günther, 1876 "1875" is transferred to Ghatophryne gen. nov., a genus of torrentially adapted toads that are endemic to the Western Ghats of India. On the basis of close morphological resemblance and distribution, Ansonia rubigina Pillai and Pattabiraman, 1981 is provisionally transferred to this new genus. The Western Ghats endemic toad Bufo koynayensis Soman, 1963 is transferred to a new genus Xanthophryne gen. nov. Based on molecular and morphological evidence, we additionally describe a new species, Xanthophryne tigerinus sp. nov., from Amboli in the Western Ghats. CONCLUSION: The descriptions and subsequent taxonomic changes we propose result in three genera of bufonids recognised as being endemic to the Western Ghats (Ghatophryne gen. nov., Xanthophryne gen. nov. and Pedostibes), and one to Sri Lanka (Adenomus). The spatial distribution, and arrangement of these lineages at the base of Adenominae diversification, reflects their Early Neogene isolation in the Western Ghats-Sri Lanka hotspot.
RESUMO
BACKGROUND: High taxonomic level endemism in the Western Ghats-Sri Lanka biodiversity hotspot has been typically attributed to the subcontinent's geological history of long-term isolation. Subsequent out of -- and into India dispersal of species after accretion to the Eurasian mainland is therefore often seen as a biogeographic factor that 'diluted' the composition of previously isolated Indian biota. However, few molecular studies have focussed on into-India dispersal as a possible source of endemism on the subcontinent. Using c. 6000 base pairs of mitochondrial and nuclear DNA, we investigated the evolutionary history and biogeography of true toads (Bufonidae), a group that colonized the Indian Subcontinent after the Indo-Asia collision. RESULTS: Contrary to previous studies, Old World toads were recovered as a nested clade within New World Bufonidae, indicating a single colonization event. Species currently classified as Ansonia and Pedostibes were both recovered as being non-monophyletic, providing evidence for the independent origin of torrential and arboreal ecomorphs on the Indian subcontinent and in South-East Asia. Our analyses also revealed a previously unrecognized adaptive radiation of toads containing a variety of larval and adult ecomorphs. Molecular dating estimates and biogeographic analyses indicate that the early diversification of this clade happened in the Western Ghats and Sri Lanka during the Late Oligocene to Early Miocene. CONCLUSION: Paleoclimate reconstructions have shown that the Early Neogene of India was marked by major environmental changes, with the transition from a zonal- to the current monsoon-dominated climate. After arrival in the Western Ghats-Sri Lanka hotspot, toads diversified in situ, with only one lineage able to successfully disperse out of these mountains. Consequently, higher taxonomic level endemism on the Indian Subcontinent is not only the result of Cretaceous isolation, but also of invasion, isolation and radiation of new elements after accretion to the Eurasian mainland.