Hybrid packed bed bioreactor using combined biodegradation and ozonation to enhance nitrogen and micropollutants removal from landfill leachate.

Landfill leachate contains ammonium and micropollutants. Ammonium can be biologically removed but bio-recalcitrant micropollutants removal requires post-treatment like ozonation. This study developed an expanded clay aggregates packed biofilm column (EBC) and demonstrated its feasibility of coupling biodegradation and ozonation (CBAO) to simultaneously remove nitrogen and bio-recalcitrant micropollutants. The first 60 days only had biodegradation process to start the bioreactor. 51 % nitrogen was biologically removed but the removal of micropollutant carbamazepine (CBZ) was only 30 %. From 61 d to 150 d, both biodegradation and ozonation were performed in the EBC. After 48 h-biodegradation, ozone gas was introduced and bubbling through EBC for 30 min to further remove residual micropollutants. At 0.4 gO3/gCOD, CBZ were completely removed. The average nitrogen removal efficiency (85 %) was increased by 34 % because the increased abundance of nitrifying and denitrifying bacteria in EBC. This study confirmed the promising potential of the CBAO process for treating landfill leachte.

Lab-scale evaluation of Microalgal-Bacterial granular sludge as a sustainable alternative for brewery wastewater treatment.

Microalgal-bacterial granular sludge (MBGS) could offer a sustainable alternative to traditional aerobic methods in brewery wastewater (BWW) treatment. This study compared MBGS with conventional activated sludge (AS) in treating real BWW and highlighted its advantages and challenges. MBGS achieved comparable chemical oxygen demand removal efficiency (93%) compared to AS (89%). Additionally, MBGS exhibited higher phosphate removal capabilities than AS. Extra nitrogen was added to influent to balance C/N ratio of BWW. MBGS was robust in handling C/N ratio fluctuations with an 82% total nitrogen removal efficiency. Metagenomic analysis further indicated that most of the genes involved in carbon, nitrogen and phosphorus metabolism were up-regulated in MBGS compared to AS. Despite changes in the microbial community and settling ability due to high starch and sugar content in BWW, MBGS demonstrated high efficiency and sustainability. Further research should optimize MBGS operation strategies to fully realize its potential for sustainable BWW treatment.

Coagulation in combination with anaerobic digestion for enhancement of resource recovery from faecal sludge.

Poorly managed faecal sludge (FS) poses significant challenges to public health and the environment. Anaerobic digestion (AD) of FS provides an effective method for energy recovery while reducing FS associated threats. Recognizing the critical role of the dewatering process before AD, this study investigates the synergistic application of chemical coagulation and mesophilic AD for synthetic FS treatment. FeCl3, AlCl3, Fe2(SO4)3, poly ferric sulfate (PFS) and poly aluminium ferric chloride (PAFC) were utilized at varying dosages to examine their impact on FS properties and subsequent biogas production from the dewatered FS. It was found that coagulation enhances sedimentation efficiencies and dewaterability through mechanisms such as charge neutralization, charge patching and bridging, thereby improving the FS feasibility for AD. Notably, polymer coagulant PFS showed good performance in balancing pollutant removal and methane recovery, contributing to facilitating the hydrolysis and acidogenesis microorganisms involved in the AD process. Optimal dosage was identified at 150 mg/g TS (1.7 g/L FS), achieving prominent removal efficiencies for total COD (67%), turbidity (85%), and total phosphorus (60%), while simultaneously enhancing AD performance with specific CH4 production reaching 517 ml CH4/g VS or 24.8 ml CH4/g AD wet feedstock compared to 309 ml CH4/g VS or 2.7 ml CH4/g AD wet feedstock in untreated FS.

Removal of organic micropollutants from water by adsorption on thermo-plasma expanded graphite encapsulated into calcium alginate.

Nowadays, public concern is focused on the degradation of water quality. For this reason, the development of innovative technologies for water treatment in view of (micro)pollutant removal is important. Indeed, organic (micro)pollutants, such as pharmaceuticals, herbicides, pesticides and plasticizers at concentration levels of µg L-1 or even ng L-1 are hardly removed during conventional wastewater treatment. In view of this, thermo-plasma expanded graphite, a light-weight innovative material in the form of a powder, was encapsulated into calcium alginate to obtain a granular form useful as filtration and adsorption material for removal of different pollutants. The produced material was used to remove atrazine, bisphenol-A, 17-α-ethinylestradiol and carbamazepine (at concentration levels of 125, 250 and 500 µg L-1) by top-down filtration. The effect of flow rate, bed depth and adsorbent composition was evaluated based on breakthrough curves. The experimental data was analysed with the Adams-Bohart model in view of scale-up. Under optimal conditions, removal and adsorption capacity of respectively about 21%, 21%, 38%,42%, 43 µg g-1, 44 µg g-1, 37 µg g-1 and 87 µg g-1 were obtained for atrazine, bisphenol, 17-α ethinylestradiol and carbamazepine when using 0.12 g of thermo-plasma expanded graphite to treat 200 mL at 500 µg L-1 (for each compound) of solution obtaining at contact time of 20 min. The granular form of TPEG obtained (GTPEG) by entrapping in calcium alginate results to have a good adsorbent property for the removal of carbamazepine, atrazine, bisphenol A and 17-α ethinylestradiol from water at concentration levels between 250 and 500 µg L-1. Promising results confirm the adsorbent properties of TPEG and push-up us to investigate on its application and improve of its performance by evaluating different entrapping materials. Supplementary Information: The online version contains supplementary material available at 10.1007/s40201-023-00876-9.

Electrocoagulation flotation as a municipal wastewater (pre-)treatment technology: Effect of weather conditions and current density.

Electrocoagulation (EC) is a promising compact alternative technology, despite its viability in municipal wastewater treatment (MWWT) is currently challenged by its energy-intensive and batch-mode operation. This study introduces an innovative continuous electrocoagulation flotation (ECF) design for MWWT. ECF shows promising pollutant removal efficiencies, with identical results using both iron (Fe) and aluminum (Al) anodes. At a current density (CD) of 120 A/m2, it achieved significant removals: 90% tCOD, 98% TP, 94% TSS, 60% BOD5, and 40% TN. Designed ECF is proposed as a pre-treatment step due to limited TN removal. The study investigated optimal ECF performance under varying weather conditions using CD ranges of 40, 80, and 120 A/m2. Both Fe and Al ECF outperformed in treating rainy weather (RW) and dry weather (DW) municipal wastewater (MWW). However, Al anode's super-faradaic behavior resulted in higher residual concentrations in effluent, (i.e., an average of 6.53-33.7 mg/L), and operational costs compared to Fe ECF. Optimized Fe ECF setting needs to be changed depending in the weather variation. Fe ECF achieved high removal rates for tCOD (94%) and TP (95%) in RW MWW at a low CD of 40 A/m2. Comparative to this, the optimum CD for treated DW MWW was between 40 and 80 A/m2, removing tCOD (71-73%) and TP (85-95%). Specifically, at these conditions, the operational expenses were respectively 0.47 ± 0.03 €/m3 (RW MWW), and 0.37 ± 0.02 €/m3 to 0.81 ± 0.04 €/m3 (DW MWW). Moreover, ECF enables resource recovery and a circular economy through anaerobic sludge digestion, with Fe ECF generating more biogas than Al.

Degradation of micropollutants in secondary wastewater effluent using nonthermal plasma-based AOPs: The roles of free radicals and molecular oxidants.

Emerging micropollutants (µPs) appearing in water bodies endanger aquatic animals, plants, microorganisms and humans. The nonthermal plasma-based advanced oxidation process is a promising technology for eliminating µPs in wastewater but still needs further development in view of full-scale industrial application. A novel cascade reactor design which consists of an ozonation chamber preceding a dielectric barrier discharge (DBD) plasma reactor with a falling water film on an activated carbon textile (Zorflex®) was used to remove a selection of µPs from secondary municipal wastewater effluent. Compare to previous plasma reactor, molecular oxidants degraded micropollutants again in an ozonation chamber in this study, and the utilization of different reactive oxygen species (ROS) was improved. A gas flow rate of 0.4 standard liter per minute (SLM), a water flow rate of 100 mL min-1, and a discharge power of 25 W are identified as the optimal plasma reactor parameters, and the µP degradation efficiency and electrical energy per order value (EE/O) are 84-98% and 2.4-5.3 kW/m³, respectively. The presence of ROS during plasma treatment was determined in view of the µPs removal mechanisms. The degradation of diuron (DIU), bisphenol A (BPA) and 2-n-octyl-4-isothiazolin-3-one (OIT) was mainly performed in ozonation chamber, while the degradation of atrazine (ATZ), alachlor (ALA) and primidone (PRD) occurred in entire cascade system. The ROS not only degrade the µPs, but also remove nitrite (90.5%), nitrate (69.6%), ammonium (39.6%) and bulk organics (11.4%). This study provides insights and optimal settings for an energy-efficient removal of µPs from secondary effluent using both free radicals and molecular oxidants generated by the plasma in view of full-scale application.

Improving the ozone-activated peroxymonosulfate process for removal of trace organic contaminants in real waters through implementation of an optimized sequential ozone dosing strategy.

The ozone-activated peroxymonosulfate process (O3/PMS) has received increasing attention for the removal of trace organic contaminants (e.g. pesticides and pharmaceuticals) from water bodies. However, the ozone dosing strategy has not yet been properly investigated, especially in real water matrices. Typically, one-step dosing is applied in literature. Nevertheless, optimal dosing is an important step for improving the process. This study investigates the effect of sequential ozone dosing on the PMS activation, atrazine (ATZ) removal, residual ozone concentration and radical exposure, and compares the results to those of a one-step ozone dosing approach. Experiments were performed in three water matrices with a different (in)organic content, i.e. secondary effluent, surface water and groundwater. In all matrices, the highest PMS activation was reached when applying three sequential ozone doses (3 × 5 mg O3/L). This resulted in a 3 times higher ATZ removal efficiency (up to 46 %) in secondary effluent compared to that obtained with a one-step ozone dosing (15 mg O3/L). In surface water and groundwater, similar ATZ removal (>90 %) was observed among the different ozone dosing strategies. However, the sulfate radical (SO4●-) exposure increased after each ozone addition. After three ozone additions of 5 mg/L, SO4●- contributed for 9 %, 26 % and 54 % to ATZ removal in respectively secondary effluent, surface water and groundwater. This high SO4●- contribution compared to ●OH contribution is an advantage as the selectivity of SO4●- gives rise to less radical scavenging by bulk organic matter and thus increases the (cost-)effectiveness of the O3/PMS process.

Life cycle assessment of microalgae systems for wastewater treatment and bioproducts recovery: Natural pigments, biofertilizer and biogas.

The aim of this study was to assess the potential environmental impacts associated with microalgae systems for wastewater treatment and bioproducts recovery. In this sense, a Life Cycle Assessment was carried out evaluating two systems treating i) urban wastewater and ii) industrial wastewater (from a food industry), with the recovery of bioproducts (i.e. natural pigments and biofertilizer) and bioenergy (i.e. biogas). Additionally, both alternatives were compared to iii) a conventional system using a standard growth medium for microalgae cultivation in order to show the potential benefits of using wastewater compared to typical cultivation approaches. The results indicated that the system treating industrial wastewater with unialgal culture had lower environmental impacts than the system treating urban wastewater with mixed cultures. Bioproducts recovery from microalgae wastewater treatment systems can reduce the environmental impacts up to 5 times compared to a conventional system using a standard growth medium. This was mainly due to the lower chemicals consumption for microalgae cultivation. Food-industry effluent showed to be the most promising scenario for bioproducts recovery from microalgae treating wastewater, because of its better quality compared to urban wastewater which also allows the cultivation of a single microalgae species. In conclusion, microalgae wastewater treatment systems are a promising solution not only for wastewater treatment but also to boost the circular bioeconomy in the water sector through microalgae-based product recovery.

Generation of environmental persistent free radicals (EPFRs) enhances ecotoxicological effects of the disposable face mask waste with the COVID-19 pandemic.

A large amount of disposable plastic face masks (DPFs) is produced and used during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, which results in an inevitable consequence of the dramatic increase of DPFs waste. However, the impact of DPFs exposure to the environment on their toxicity is rarely considered. In this study, a range of 76-276 items/L microplastics (MPs) was detected in the DPFs leachates, and fibrous (> 80.3%) and polypropylene (PP, > 89.2%) MPs were dominant. Co, Cu, Ni, Sr, Ti and Zn, were commonly detected in all leachates of the tested DPFs. Organics, such as acetophenone, 2,4-Di-tert-butylphenol, benzothiazole, bisphenol-A and phthalide, were found in the DPFs leachate, which were including organic solvents and plasticizer. Besides, we first found an emerging environmental risk substance, namely environmentally persistent free radicals (EPFRs), was generated in the DPFs leachates. The characteristic g-factors of the EPFRs was in a range of 2.003-2.004, identified as mixture of carbon- and oxygen-centered radicals. By means of in vitro toxicity assay, the DPFs leachate were confirmed to cause cytotoxicity and oxidative stress. Significantly, it is found that the formed EPFRs could contribute more toxic effects. Furthermore, when compared to N95 respirators, the tested surgical masks tend to release more MPs, leach more metals and organics, and generate more EPFRs. Surgical masks were thus showed higher risk than N95 respirators after exposure to water. This work highlights the importance of understanding the chemical complexity and possible toxicity of DPFs for their risk assessment.

Advanced treatment of landfill leachate through combined Anammox-based biotreatment, O3/H2O2 oxidation, and activated carbon adsorption: technical performance, surrogate-based control strategy, and operational cost analysis.

The complexity of landfill leachate makes it difficult to treat it with a single biological/ physical/chemical process. Moreover, the dynamic leachate characteristics pose a challenge for effective process control. Therefore, a combined treatment, consisting of a one-stage partial nitrification-Anammox process, an O3/H2O2 process, and a granular activated carbon filtration (GAC) process, was investigated. Meanwhile, a novel surrogate-based ozone dose control strategy for O3/H2O2 process was evaluated. Results show that this three-stage process offers high removal of total nitrogen (> 90%), COD (chemical oxygen demand, 60-82%), and micropollutants (atrazine, alachlor, carbamazepine, and bisphenol A, > 96%), satisfying discharge requirements. In the combined post-treatment, ozone dosing for COD removal can be real-time controlled by UVA254 reduction monitoring, based on a specific correlation between COD and UVA254 changes. On the other hand, O3/H2O2 pre-treatment controlled at a 50% UVA254 reduction shows to be the optimal point, when adsorption is designed as the main step for COD removal. Cost analysis shows that post-treatment with low (high) organic load i.e., COD ≤ (≥)540 mg/L, a combination with O3/H2O2 (GAC) as the main step appears to be more cost-effective. Therefore, a dynamic operation strategy in response to the leachate change is recommended.

Surrogate-based follow-up of activated carbon adsorption preceded by ozonation for removal of bulk organics and micropollutants from landfill leachate.

Although combined ozonation with activated carbon (AC) adsorption is a promising technique for leachate treatment, little is known about how ozone-induced changes in leachate characteristics affect the organics adsorption, especially in view of emerging micropollutants (MPs) removal. Furthermore, the online monitoring of MPs is challenging but desirable for efficient treatment operation. This study investigates how preceding ozonation impacts the adsorption of bulk organics (expressed as chemical oxygen demand (COD)) and ozone-recalcitrant MPs, i.e., primidone, atrazine and alachlor, in leachate using batch and column adsorption tests. Additionally, a new surrogate-based model was evaluated for predicting MPs breakthrough. Batch tests revealed that ozonation results in a decreasing apparent affinity of COD towards AC, but the non-adsorbable part did not obviously change. The adsorption of MPs in ozonated leachate was (1-41%) higher than that in non-ozonated leachate, especially for the more hydrophobic alachlor and atrazine, due to a reduced sites competition from bulk organics. Column adsorption showed that ozonation delayed COD and MPs breakthrough due to the reduced COD loading and sites competition, respectively. An increased empty bed contact time (EBCT, 10-40 min) led to an increased COD uptake by a factor of 3.0-3.2 for ozonated and non-ozonated leachates, while MPs adsorption also increased, suggesting that pore blockage rather than site competition could be the dominant inhibitory effect. The data from column adsorption demonstrate the applicability of developed surrogate-based model for predicting MPs breakthrough. Particularly, the fitting parameters were not affected by change of leachate characteristics, while they were impacted by change of EBCT.

Performance of a green wall (Total Value Wall™) at high greywater loading rates and Life Cycle Impact Assessment.

Nature-based greywater (GW) treatment and reuse in urban areas has become an up-and-coming option. A 14.4 m2 green wall system called Total Value Wall (TVW) was installed at a terraced house in Gent (Belgium) for treating GW and reusing the effluent for toilet flushing. In a previous study, the TVW was loaded at 7 L.m-2.d-1 and efficiently removed TSS (67%), COD (43%), BOD5 (83%) and total coliforms (log 2), but a number of issues were reported related to nutrient leaching from the substrate, and the excessive retention time in the storage tanks. In this study results are reported from a follow-up study during which an adapted TVW was subjected to both higher hydraulic and pollutant loading rates in order to investigate the treatment capability of TVW. The design of the system, i.e. substrate contained in geotextile bags, did not sustain the higher hydraulic loading rates as excessive leakage occurred. Despite this, the higher pollutant loading rates still resulted in an acceptable effluent quality with 15 mg.L-1 TSS (90%), 85 mg.L-1 COD (82%), and 15 mg.L-1 BOD5 (95%). Ammonium, E. coli and total coliforms were removed with removal rates of 98%, 63% (0.4 log units), and 36% (0.2 log units), respectively. Finally, a life cycle assessment (LCA) was performed for the TVW with and without treating GW to analyze the environmental burden. The LCA impacts showed that replacing tap water and chemical fertilizer by GW, and the reuse of effluent, have a positive impact. However, the energy use for pumping has a major impact and should be minimized by using an efficient pump and distribution system to reduce the overall footprint.

Enhanced removal of refractory humic- and fulvic-like organics from biotreated landfill leachate by ozonation in packed bubble columns.

Biotreated landfill leachate contains much refractory organics such as humic and fulvic acids, which can be degraded by O3. However, the low O3 mass transfer and high energy cost limit its wide application in landfill leachate treatment. Previous studies proved that packed bubble columns could enhance the O3 mass transfer and increase the synthetic humic acids wastewater degradation, but the performance of packed bubble columns in real wastewater treatment has not been investigated. Therefore, this study aims to evaluate the feasibility of application of packed bubble column in the real biotreated landfill leachates treatment and provide insights into the transformation of organic matters in leachates during ozonation. Packed bubble columns with lava rocks or metal pall rings (LBC or MBC) were applied and compared with a non-packed bubble column (BC). At an applied O3 dose of 8.35 mg/(Lwater sample min), the initial COD (400 mg/L) was only removed for 26% in BC and 32% in MBC while this was 46% in LBC, indicating LBC has the best performance. GC-MS analysis shows that raw biotreated leachate contains potential endocrine disruptors such as di(2-ethylhexyl) phthalate (DEHP). 61% of DEHP was removed in LBC and the least intermediate oxidation products from humic- and fulvic-like organics was detected in LBC. The highest O3 utilization efficiency (89%) and hydroxyl radical (OH) exposure rate (3.0 × 10-10 M s) were observed in LBC with lowest energy consumption (EEO) for COD removal of 18 kWh/m3. The enhanced ozonation efficiency in LBC and MBC was attributed to the improved O3 mass transfer. Besides, LBC had additional adsorptive and catalytic activity that promoted the decomposition of O3 to generate OH. This study demonstrates that a packed bubble column increases removal and decreases energy use when treating landfill leachate, thus promoting the application of ozonation.

Towards a general kinetic microalgae model: Extending a semi-deterministic green microalgae model for the cyanobacterium Arthrospira platensis and red alga Porphyridium purpureum.

Mathematical models for microalgae and cyanobacteria are seldomly validated for different algal species, as such limiting their applicability. Therefore, in this research, a previously developed kinetic model describing the growth of the green microalgae species Chlorella vulgaris was used to simulate the growth of the cyanobacterium Arthrospira platensis and the red alga Porphyridium purpureum. Based on a global sensitivity analysis, the model parameter µmax,A was calibrated using respirometric-titrimetric data. Calibration yielded values of 5.76 ± 0.17 d-1, 2.06 ± 0.16 d-1 and 1.06 ± 0.09 d-1 for Chlorella vulgaris, Arthrospira platensis and Porphyridium purpureum, respectively. Model simulations revealed that the biological growth equations in this model are adequate. However, increased light intensities triggered a survival mechanism for Arthrospira platensis, which is currently not taken into account by the model, leading to bad model accuracy under these circumstances. Future work should address the most important survival mechanisms and include those in the model to widen its applicability.

Intensified ozonation in packed bubble columns for water treatment: Focus on mass transfer and humic acids removal.

Ozonation has been widely applied for the oxidation of contaminants in wastewater, and the disinfection of water. However, low ozone (O3) mass transfer efficiency in common ozonation reactors requires high O3 doses and causes high energy consumption. In this study, to intensify the O3 mass transfer and oxidation of humic acids (HA) solution, a lava rock packed bubble column (LBC) and a metal pall ring packed bubble column (MBC) were developed and evaluated. In comparison with non-packed bubble column (BC), both LBC and MBC enhanced the O3 mass transfer efficiency and the generation of hydroxyl radicals, thereby increasing the HA removal from an aqueous solution. At applied O3 dose of 33.3 mg/(Lcolumn h), the HA removal efficiency in BC was only 47%. When MBC and LBC were applied, it increased to 66% and 72%, respectively. Meanwhile, the O3 utilization efficiency in LBC reached 68%, which was higher than that in MBC (50%) and BC (21%). Consequently, LBC has the lowest energy consumption (EEO) for HA removal (1.4 kWh/m3), followed by MBC (1.6 kWh/m3) and BC (2.9 kWh/m3). LBC had better performance than MBC due to the adsorptive and catalytic roles of lava rock on the ozonation process. This study demonstrates the advantages of using lava rocks as packed materials in O3 bubble column over metal pall rings in intensifying O3 mass transfer and organic matters removal, which provides some insights into promoting the industrial application of O3.

Total value wall: Full scale demonstration of a green wall for grey water treatment and recycling.

Greywater treatment and reuse for non-potable purposes in urban areas has become a widely researched topic to reduce the burden on fresh water resources. This study reports on the use of a green wall for treating grey water and reusing the effluent for toilet flushing, called Total Value Wall (TVW). Initially, the effectiveness of (mixtures of) different substrates, i.e. lava, lightweight expanded clay aggregates, organic soil and biochar was investigated by means of column tests. All substrates were first examined for hydraulic characteristics and later on the columns were fed with synthetic grey wastewater and followed up in terms of removal efficiency of COD and detergents. The mixture consisting of lava (50%), organic soil (25%) and biochar (25%) proved to be optimal both in terms of percolation rates and removal efficiencies, and was thus selected for the full-scale system. The full-scale TVW of 14.4 m2 was installed at a terraced house in Ghent (Belgium), and was loaded with grey water at 100 L per day. Influent and effluent quality were routinely monitored by grab sampling, water savings were monitored by means of flow meters, and electricity consumption was also accounted for. The TVW was further equipped with sensors that measure temperature, Particulate Matter (PM10) and CO2 in the air. The full-scale system obtained effluent concentrations of 13 mg.L-1 TSS, 91 mg.L-1 COD and 5 mg.L-1 BOD5. Ammonium and total coliforms were removed with removal rates of 97% and 99% (2 log units) respectively. However, an increase in effluent concentration of nitrate and phosphate was observed due to leaching from the selected substrate. Available data from the temperature sensors have clearly demonstrated the additional benefit of the TVW as an insulating layer, keeping the heat outside on warmer days, and keeping the heat inside on colder days. Overall, this study demonstrated that the TVW is a sustainable system for greywater treatment and reuse.

Roof runoff contamination: Establishing material-pollutant relationships and material benchmarking based on laboratory leaching tests.

Because roofs represent a major part of the urban impervious surface, it is hypothesized that roof runoff is an important source of urban stormwater contamination. However, the contribution of different roofing materials to this contamination has only been examined to a limited extent. In this study, a resource and time efficient methodology, which uses some of the principles of a standardized leaching test (CEN/TS16637-2), was developed to identify material-pollutant relationships for sixteen commonly used roofing materials (EPDM, PVC, TPO, EVA, PU and bitumen membranes). Metals were detected in concentrations ranging from several µg/L in the leachate of synthetic materials up to 2.5 mg/L for Zn in the leachate of EPDM materials. Cd and Cr were not detected in any of the leachates. Furthermore, polycyclic aromatic hydrocarbons were detected in most leachates, with phenanthrene and naphthalene being most frequently detected in concentrations up to 4.5 µg/L for naphthalene. Further insights on organic pollutants' leaching from the tested materials were obtained by a non-target GC-MS screening of the leachates. Several commonly used additives such as flame retardants and light stabilizers were detected. Although no information on long-term leaching and material behavior under outdoor conditions could be obtained by the developed methodology, the laboratory test results could be used to benchmark the materials for their potential impact on roof runoff quality by the calculation of material indexes (which summarize the material-pollutant relationships). EPDM and PU roofing materials were identified as the materials having the highest potential to affect roof runoff quality.

Model based analysis of carbon fluxes within microalgae-bacteria flocs using respirometric-titrimetric data.

With the emerging need of nutrient recycling in resource recovery facilities, the use of microalgae-bacteria flocs has received considerable attention in the past few years. However, although the main biological processes are already known, the complex interactions occurring between algae and bacteria are not fully understood. In this work, a combined respirometric-titrimetric unit was used to assess the microorganisms' kinetics within microalgae-bacteria flocs under different growth regimes (i.e. photoautotrophic, heterotrophic and mixotrophic) and different ratios of inorganic (IC) to organic carbon (OC) (IC:OC-ratios). Using this respirometric-titrimetric data, a new model was developed, calibrated and successfully validated. The model takes into account the heterotrophic growth of bacteria, the photoautotrophic, heterotrophic and mixotrophic growth of algae and the production and consumption of extracellular polymeric substances (EPS) by both bacteria and algae. As such, the model can be used for detailed analysis of the carbon fluxes within microalgae-bacteria flocs in an efficient way. Model analysis revealed the high importance of the EPS regulatory mechanism. Firstly, under heterotrophic growth conditions, OC-uptake occurred during the first 10-15 min. This was linked with internal OC storage (49% of added OC) and EPS production (40%), as such providing carbon reserves which can be consumed during famine conditions. Moreover, the algae were able to compete with bacteria for OC. Secondly, under photoautotrophic conditions, algae used the added IC to grow (57% of added IC) and to produce EPS (29%), which consecutively stimulated heterotrophic bacteria growth (20%). Finally, under mixotrophic conditions, low IC:OC-ratios resulted in an extensive OC-storage and EPS production (50% of added C) and an enhanced microalgal CO2 reuse, resulting in an increased algal growth of up to 29%.

Fate and removal of microplastics in unplanted lab-scale vertical flow constructed wetlands.

Many studies have reported conventional wastewater treatment plants as one of the main sources of microplastics (MPs). However, constructed wetlands (CWs) as a nature-based wastewater treatment system have received little attention. This study investigated the influence of biofilm, media type and earthworms on the fate and removal of MPs in a short-term (45d) experiment with unplanted lab-scale vertical flow CWs (VFCWs). In sand-filled VFCWs, MPs were retained in the first 10 cm, and the removal efficiency was 100%, regardless of the presence of a biofilm. When gravel was used as filling material, the removal efficiency of MPs was stable at 96%, but the MPs were distributed throughout the 80 cm high VFCWs. In the presence of earthworms, the maximum depth that MPs reached within sand-filled VFCWs increased from 10 to 15 cm. Furthermore, the MPs concentration at a depth of 3-6 cm and 6-10 cm increased 2 and 10 fold respectively compared to the same VFCWs without earthworms. Although no MPs were detected in the sand from deep layers (15-80 cm), transport of MPs from top to the bottom by earthworms was found, and a few MPs were detected in the effluent, leading to a removal efficiency of 99.8%. This study indicated that both a higher media grain size and the presence of earthworms have a small effect on the removal efficiency of MPs in VFCWs, but the effect on the distribution of MPs was considerably. Longer-term studies in full-scale CWs are advised to perform under the influence of more practical factors.

Natural Pigments and Biogas Recovery from Microalgae Grown in Wastewater.

This study assessed the recovery of natural pigments (phycobiliproteins) and bioenergy (biogas) from microalgae grown in wastewater. A consortium of microalgae, mainly composed by Nostoc, Phormidium, and Geitlerinema, known to have high phycobiliproteins content, was grown in photobioreactors. The growth medium was composed by secondary effluent from a high rate algal pond (HRAP) along with the anaerobic digestion centrate, which aimed to enhance the N/P ratio, given the lack of nutrients in the secondary effluent. Additionally, the centrate is still a challenging anaerobic digestion residue since the high nitrogen concentrations have to be removed before disposal. Removal efficiencies up to 52% of COD, 86% of NH4 +-N, and 100% of phosphorus were observed. The biomass composition was monitored over the experimental period in order to ensure stable cyanobacterial dominance in the mixed culture. Phycocyanin and phycoerythrin were extracted from harvested biomass, achieving maximum concentrations of 20.1 and 8.1 mg/g dry weight, respectively. The residual biomass from phycobiliproteins extraction was then used to produce biogas, with final methane yields ranging from 159 to 199 mL CH4/g VS. According to the results, by combining the extraction of pigments and the production of biogas from residual biomass, we would not only obtain high-value compounds, but also more energy (around 5-10% higher), as compared to the single recovery of biogas. The proposed process poses an example of resource recovery from biomass grown in wastewater, moving toward a circular bioeconomy.