Physiological and biochemical response in green mussel Perna viridis subjected to continuous chlorination: Perspective on cooling water discharge criteria.

Heavy infestation by Perna viridis has been observed in the sub-seabed seawater intake tunnel and CWS of a tropical coastal power station in-spite of continuous low dose chlorination regime (0.2 ± 0.1 mg L-1) (CLDC), indicating periodical settlement and growth. Continuous arrival of mussels (colonized in the sub seabed tunnel intake section) at the pump house indicated that the mussels were able to tolerate and survive in a chlorinated environment, for varying time periods and were dislodged when they become weak and subsequent death, leading to flushing out of the system. In the present study, effect of continuous chlorination [0.2 mg L-1 (in-plant use); 0.5 mg L-1 (shock dose) & 1.0 mg L-1 (high levels)] was evaluated on mussels to assess; (a) time taken for mortality, (b) action of chlorine on physiological, genetic, metabolic and neuronal processes. 100% mortality of mussels was observed after 15 (0.2 mg L-1); 9 (0.5 mg L-1) and 6 days (1.0 mg L-1) respectively. Extended valve closure due to chlorination resulted in stress, impairing the respiratory and feeding behavior leading to deterioration in mussel health. Pseudofaeces excretion reduced to 68% (0.2 mg L-1); 10% (0.5 mg L-1) and 89% (1.0 mg L-1) compared to controls. Genotoxicity was observed with increase in % tail DNA fraction in all treatments such as 86% (0.2 mg L-1); 76% (0.5 mg L-1) and 85% (1.0 mg L-1). Reactive Oxygen Species (ROS) stress biomarkers increased drastically/peaked within the first 3 days of continuous chlorination with subsequent quenching by antioxidant enzymes. Gill produced highest generation of ROS; 38% (0.2 mg L-1); 97% (0.5 mg L-1); 98% (1.0 mg L-1). Additionally, it was shown that 84% (0.2 mg L-1), 72% (0.5 mg L-1), and 80.4% (1.0 mg L-1) of the neurotransmitter acetylcholinesterase activity was inhibited by chlorine at the nerve synapse. The cumulative impact of ROS generation, neuronal toxicity, and disrupted functions weakens the overall health of green mussels resulting in mortality.

Dynamics of soil penetration resistance, moisture depletion pattern and crop productivity determined by mechanized cultivation and lifesaving irrigation in zero till blackgram.

Rice fallow black gram is grown under the residual moisture situation as a relay crop in heavy texture montmorillonite clay soil under zero till condition. Since the crop is raised during post monsoon season, the crop often experiences terminal stress due to limited water availability and no rainfall. Surface irrigation in montmorillonite clay soil is determent to pulse crop as inundation causes wilting. Therefore, zero tilled rice fallow black gram has to be supplemented with micro irrigation at flowering stage (35 days after sowing) to alleviate moisture stress and to increase the productivity as well. Hence micro farm pond in a corner of one ha field was created to harvest the rain water during monsoon season and the same was utilized to supplement the crop with lifesaving irrigation through mobile sprinkler at flowering stage for the crop grown under conservation agriculture. Soil cracking is also the common phenomena of montmorillonite clay soil where evaporations losses would be more through crack surfaces. The present study was therefore conducted to study the changes in the soil physical properties, crop establishment and productivity in conjunction with mechanized sowing and harvest and supplemental mobile sprinkler irrigation. Sowing of black gram by broadcasting 10 days prior to the manual harvest of rice, manual drawn single row seed drill after the machine harvest of rice and sowing by broadcasting at 4 days prior to machine harvest of rice was experimented separately and in combination with lifesaving irrigation. Results indicated that the number of wheel passes and lifesaving irrigation had a very strong impact on soil penetration resistance and soil moisture. Combined harvester followed by no till seed drill increased the soil penetration resistance in all the layers (0-5 cm, 5-10 cm and 10-15 cm). Two passes of wheel increased the mean soil penetration resistance from 407 KPa to 502 KPa. The soil penetration resistance (0-5 cm) at harvest shown that black gram sown by manual broadcasting 10 days prior to manual harvest of paddy supplemented with life irrigation on 30 DAS reduced the soil penetration resistance from 690 Kpa to 500 Kpa, 740 Kpa to 600 Kpa and 760 Kpa to 620 Kpa respectively at 0-5 cm, 5-10 cm and 10-15 cm layer. In general, moisture depletion rate was rapid in the surface layer of 0-5 cm as compared to other layers of 5-10 cm and 10-15 cm up to 30 DAS (Flowering stage). The moisture content and the soil penetration resistance had an inverse relationship. The soil penetration resistance also had an inverse relationship with the root length in which the root length lowers as the soil penetration resistance increases. The soil crack measured at 60 DAS was deeper with no till seed drill (width of 3.94 cm and depth of 13.67 cm) which was mainly due to surface layer compaction. The relative water content, specific leaf weight and chlorophyll content were significantly improved through the supplemental irrigation given on 30 DAS irrespective of crop establishment methods. The results further indicated that compaction of ploughed layer in the moist soil due to combined harvester and no till seed drill had a negative impact on yield (457 kg ha-1), which was improved by 19.03 per cent due to increased soil moisture with supplemental irrigation. The mean yield increase across different treatments due to supplemental lifesaving irrigation through mobile sprinkler was 20.4 per cent.

Efficacy and Safety outcomes of a novel model to assess new medical retina referrals in a high-volume medical retina virtual clinic.

BACKGROUND: Ophthalmology outpatient attendances have significantly increased recently with rising pressure from backlogs arising from the pandemic. Medical retina digital surveillance clinics for stable follow-up appointments are well established. We present a model for assessing new referrals and evaluating clinical outcomes and long-term sustainability in a complex high-volume medical retina service. METHODS: Suitable routine new patient referrals were identified from electronic referrals and referred to this new pathway. Structured history, visual acuities, and intraocular pressures were recorded, and widefield colour fundus and optical coherence tomography imaging were performed at a imaging hub for asynchronous consultant-led review. RESULTS: 1458 patients were invited to attend over four months, with a 13.2% did-not-attend (DNA) rate. Common diagnoses included stable diabetic retinopathy (19.9%), early age-related macular degeneration (6.7%), central serous retinopathy (8.8%), and retinal vein occlusion (6.3%). 7 patients (0.05%) required urgent same-day review. 61 (5.0%) required urgent face-to-face (F2F) assessment within two weeks. A total of 727 (59.0%) were either discharged or remained in the virtual pathway following their first visit. CONCLUSION: This study encourages the use of a digital model that efficiently assesses suitable newly referred medical retina patients in both complex and local eye unit settings. This decreased the need for F2F clinics and resources. Further patient satisfaction surveys for digital services are currently being evaluated to guide long-term sustainability of this model.

Assessment of biogrowth assemblages with depth in a seawater intake system of a coastal power station.

Marine biogrowth infestation of a seawater intake system was investigated. A digital camera fixed onto a skid was used to record the biogrowth at intervals of 5 m up to a depth of 55 m. Divers inspected the intake shaft and collected the biogrowth samples for biomass estimation. A biomass density of 7.5 kg m-2 and 28.2 kg m-2 was recorded at 5 and 30 m depths respectively. Inspection by the divers revealed that hard-shelled organisms such as oysters and brown and green mussels were observed in plenty up to a thickness of 15 cm and bryozoans grew as epibionts. At lower depths (<40 m), hydroids grew on the shells of green mussels along with silt accumulation. The biofouling community was composed of 46 organisms, exhibiting variation in distribution and abundance. The study explains the extent and type of marine biogrowth phenomena with depth and describes biofouling preventive methods.Supplemental data for this article is available online at https://doi.org/10.1080/08927014.2021.1933457 .

Response of green mussels (Perna viridis) subjected to chlorination: investigations by valve movement monitoring.

Perna viridis Linnaeus (1758) is a major foulant in the cooling water systems of electric power stations located on the East coast of India. Though chlorination is considered an effective fouling control measure, the strategy may fail in the case of bivalve mussels, due to the ability of the mussels to close their shells and still survive for extended periods of time. In a given power station, continuous low dose (exomotive) chlorination (0.2 ± 0.1 mg l-1) is practiced to control biofouling. Laboratory experiments were carried out to assess the mortality and valve movement response of Perna viridis exposed to chlorine, using a Mosselmonitor®. All size groups tested showed progressive reduction in valve opening upon chlorination. However, continuous dosing of chlorine concentration as high as 1.0 mg l-1 was required for sustained and complete valve closure response in this mussel. At lower concentration (0.7 mg l-1), the mussels were able to open their shells and feed. Sustained valve closure resulted in physiological stress to the mussels due to reduced feeding, subsequently leading to death. Time to 100% mortality was dependent on the size of the mussels. At 1.0 mg l-1 chlorine residual, smaller size group (30-50 mm) mussels showed 100% mortality in 79.3 h, while larger groups (50-70 mm and 70-90 mm) took 152 h and 243 h, respectively. Frequency of valve opening was high in smaller size group mussels (30-50 mm), compared with larger groups (70-90 mm). Even though the time taken for killing was size-dependent, frequency of valve opening and time period between successive openings were found to be characteristic of individual mussels. The observations provide new insight into the response of bivalve mussels to continuous chlorination in the context of biofouling control and point to the need to adopt pragmatic strategies to prevent mussel spat settlement rather than killing of adult mussels, thereby reducing environmental burden due to chlorine residuals. Usage of target-specific biocidal strategies (intermittent/pulse dosing) or alternative biocides (chlorine dioxide) may help mitigate green mussel fouling in tropical cooling water systems.