RESUMO
Objetivo: Evaluar el efecto de una intervención de teleenfermería en contexto de pandemia para prevenir la anemia infantil en Perú. Material y Método: Estudio piloto, analítico, cuasi experimental con pre y pos-test. La muestra estuvo constituida por 60 madres de un establecimiento de atención primaria de Lambayeque, Perú, que fueron seleccionadas a conveniencia y luego asignadas al Grupo control (30) y Grupo experimental (30), este último sometido a una intervención de teleenfermería de 4 meses. El efecto se midió comparando el promedio de hemoglobina e ingesta alimentaria de hierro antes y después de la intervención. La hemoglobina fue determinada bioquímicamente a través del análisis de sangre; la ingesta alimentaria de hierro fue recogida mediante entrevistas telefónicas a través del recordatorio de 24 horas. Resultados: El promedio de hemoglobina en el grupo experimental no presentó diferencia significativa entre el inicio y el final de la intervención (p= 0,199); en el grupo control el promedio de hemoglobina presentó diferencias significativas entre el inicio y el final de la intervención (p= 0,013). El promedio de ingesta alimentaria de hierro en los niños del grupo control (p= 0,049) y experimental (p= 0,000) sí tuvieron diferencias significativas entre el inicio y el final de la intervención. Conclusión: Los niños que recibieron la intervención con teleenfermería se mantuvieron sin anemia y la ingesta alimentaria de hierro aumentó por lo que se corrobora que los programas de teleenfermería tienen el potencial de incrementar información sobre alimentación, favoreciendo la salud del niño, de la madre y la familia.
Objective: To evaluate the effect of a telenursing intervention in the context of the pandemic to prevent childhood anemia in Peru. Material and Method: Pilot, analytical, quasi-experimental study with pre- and post-test. The sample consisted of 60 mothers from a primary health care facility in Lambayeque, Peru, who were selected at convenience and then assigned to a control group (30) and an experimental group (30), with the latter being subjected to a 4 months telenursing intervention. The effect was measured by comparing the average hemoglobin and dietary iron intake before and after the intervention. Hemoglobin level was determined biochemically through blood analysis. Dietary iron intake was collected through telephone interviews following a 24-hour recall. Results: The average hemoglobin level in the experimental group did not show significant differences between the beginning and the end of the intervention (p= 0.199); whereas in the control group, the average hemoglobin level showed significant differences between the beginning and the end of the intervention (p= 0.013). The average dietary intake of iron among the children of the control group (p= 0.049) and the experimental group (p= 0.000) had a significant difference between the beginning and the end of the intervention. Conclusion: The children who received the telenursing intervention suffered no anemia and the dietary iron intake increased, thus corroborating that telenursing programs have the potential of offering more information on nutrition, favoring the health of the child, the mother and the family.
Objetivo: Avaliar o efeito de uma intervenção de telenfermagem no contexto da pandemia para prevenir a anemia infantil no Peru. Material e Método: Estudo piloto, analítico, quase-experimental com pré e pós-teste. A amostra foi composta por 60 mães de uma unidade básica de saúde de Lambayeque, Peru, que foram selecionadas por conveniência e depois designadas para um dos dois grupos de pesquisa: grupo controle=30 e um grupo experimental=30, submetido esse último a uma intervenção de telenfermagem de 4 meses. O efeito foi medido comparando a hemoglobina média e a ingestão de ferro dietético antes e depois da intervenção. O nível de hemoglobina foi determinado bioquimicamente através da análise de sangue. A ingestão de ferro dietético foi coletada através de entrevistas telefónicas por meio de um recordátorio de 24 horas. Resultados: O nível médio de hemoglobina no grupo experimental não apresentou diferenças significativas entre o início e o final da intervenção (p= 0,199). No grupo controle, a hemoglobina média mostrou diferenças significativas entre o início e o final da intervenção (p= 0,013). A ingestão alimentar média de ferro entre as crianças do grupo controle (p = 0,049) e do grupo experimental (p = 0,000) teve uma diferença significativa entre o início e o final da intervenção. Conclusão: As crianças que receberam a intervenção de teleenfermagem não sofreram anemia e a ingestão de ferro por via alimentar aumentou, corroborando assim que os programas de teleenfermagem podem oferecer mais informações sobre a nutrição, favorecendo a saúde da criança, da mãe e da família.
Assuntos
Hipersensibilidade a Drogas/diagnóstico , Hipersensibilidade a Drogas/imunologia , Acetato de Glatiramer/efeitos adversos , Imunossupressores/efeitos adversos , Adulto , Biópsia , Estudos de Casos e Controles , Feminino , Acetato de Glatiramer/administração & dosagem , Humanos , Imunossupressores/administração & dosagem , Pele/imunologia , Pele/patologiaAssuntos
Hipersensibilidade a Drogas/diagnóstico , Hipersensibilidade a Drogas/imunologia , Urticária/diagnóstico , Urticária/imunologia , Acetaminofen/efeitos adversos , Adulto , Anestésicos Locais/efeitos adversos , Anti-Inflamatórios não Esteroides/efeitos adversos , Biópsia , Feminino , Humanos , Pessoa de Meia-Idade , Testes CutâneosRESUMO
Granulocytic sarcoma or chloroma is a tumor of immature cells from the granulocyte line that is generally associated with acute myeloid leukemia. The skin is one of the most affected organs. This lesion may complicate hematological dyscrasias, which is generally indicative of a poor prognosis. We present a case of a 51-year-old patient who was diagnosed with acute myeloid leukemia with a complex karyotype that debuted with a post-transplant cutaneous and hematological relapse, a very rare occurrence in the literature given that no extramedullary involvement was present prior to the transplant.
Assuntos
Transplante de Medula Óssea/patologia , Leucemia Mieloide Aguda/patologia , Infiltração Leucêmica/patologia , Sarcoma Mieloide/patologia , Pele/patologia , Evolução Fatal , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
BACKGROUND: Rapid and consistent genotyping is an important requirement for cultivar identification in many crop species. Among them grapevine cultivars have been the subject of multiple studies given the large number of synonyms and homonyms generated during many centuries of vegetative multiplication and exchange. Simple sequence repeat (SSR) markers have been preferred until now because of their high level of polymorphism, their codominant nature and their high profile repeatability. However, the rapid application of partial or complete genome sequencing approaches is identifying thousands of single nucleotide polymorphisms (SNP) that can be very useful for such purposes. Although SNP markers are bi-allelic, and therefore not as polymorphic as microsatellites, the high number of loci that can be multiplexed and the possibilities of automation as well as their highly repeatable results under any analytical procedure make them the future markers of choice for any type of genetic identification. RESULTS: We analyzed over 300 SNP in the genome of grapevine using a re-sequencing strategy in a selection of 11 genotypes. Among the identified polymorphisms, we selected 48 SNP spread across all grapevine chromosomes with allele frequencies balanced enough as to provide sufficient information content for genetic identification in grapevine allowing for good genotyping success rate. Marker stability was tested in repeated analyses of a selected group of cultivars obtained worldwide to demonstrate their usefulness in genetic identification. CONCLUSIONS: We have selected a set of 48 stable SNP markers with a high discrimination power and a uniform genome distribution (2-3 markers/chromosome), which is proposed as a standard set for grapevine (Vitis vinifera L.) genotyping. Any previous problems derived from microsatellite allele confusion between labs or the need to run reference cultivars to identify allele sizes disappear using this type of marker. Furthermore, because SNP markers are bi-allelic, allele identification and genotype naming are extremely simple and genotypes obtained with different equipments and by different laboratories are always fully comparable.
Assuntos
Técnicas de Genotipagem , Polimorfismo de Nucleotídeo Único , Vitis/classificação , DNA de Plantas/genética , Marcadores Genéticos , Repetições de Microssatélites , Análise de Sequência de DNA/métodos , Vitis/genéticaRESUMO
BACKGROUND: We report an additional case of an extremely uncommon but distinctive histological variant of benign schwannoma, which was previously designated as neuroblastoma-like schwannoma by Goldblum et al. METHODS: A 29-year-old woman referred to a 6-year-history of an atrofic macule. Its clinical appearance was similar to that of an anetoderma. RESULTS: A cutaneous biopsy showed findings consistent with a neuroblastoma-like schwannoma with the following peculiar features: (i) Being fully composed of rosette-like structures. (ii) Association to an anetoderma. CONCLUSIONS: Because neither the histological pattern nor the type of tumor allows a differential diagnosis with neuroblastoma, we propose the descriptive term of rosetoid schwannoma. And to our knowledge, this will be the first case reported of rosetoid schwannoma associated to anetoderma.
Assuntos
Neurilemoma/patologia , Dermatopatias/patologia , Adulto , Tecido Elástico/patologia , Feminino , Humanos , Neurilemoma/química , Neurilemoma/complicações , Proteínas S100/análise , Dermatopatias/complicaçõesRESUMO
BACKGROUND AND PURPOSE: Expression of epidermal growth factor receptor (EGFR) is observed in 50-70% of colorectal carcinoma and is associated with poor prognosis. The aim of this study was to determine the prognostic value of EGFR status before radiotherapy in a group of patients with locally advanced rectal cancer treated with preoperative radiotherapy. PATIENTS AND METHODS: Eighty-seven patients were studied retrospectively. Treatment consisted of pelvic radiotherapy, in 50 patients with concomitant chemotherapy and surgical resection. Immunohistochemistry for EGFR was determined at the preradiation biopsy and in the resected specimens. Immunohistochemical analysis for EGFR expression was evaluated according to extension and staining intensity. We defined positive staining (EGFR positive), when extension was 5% or more. RESULTS: A total of 52 of 87 tumors showed EGFR positive status at biopsy (60%) and EGFR expression was associated neither with clinical tumor stage nor with clinical nodal stage. EGFR positive expression was linked to a lack of pathologic complete response to preoperative radiotherapy (P=0.006). Disease-free survival was lower among patients with EGFR positive status before radiotherapy (P=0.003). In a multivariate analysis EGFR expression at biopsy was a statistically significant predictor of disease-free survival, RR=2.88(1.1-7.8), P=0.036. CONCLUSIONS: EGFR is expressed in a significant number of rectal tumors. EGFR-positive expression before radiotherapy is an indicator for poor response and low disease-free survival.