Molecular, biochemical, and sensorial characterization of cocoa (Theobroma cacao L.) beans: A methodological pathway for the identification of new regional materials with outstanding profiles.

Cocoa is an economically important product in Colombia. On-farm germplasm evaluations enable the selection of superior genotypes for propagation and distribution across the country. This study examined 12 cocoa samples from Antioquia along with five reference materials, employing 96 single nucleotide polymorphism (SNP) markers. Furthermore, these genetic findings were correlated with physical, chemical, and sensory attributes. Primary coordinate analysis revealed that the majority of samples were hybrids derived from five original germplasm pools, including Criollo, Amelonado, and three Upper Amazon Forastero cocoas. The integral profile of the 12 selected materials was classified into Modern Criollo (Rodriguez-Medina et al., 2019) [3], Forasteros (Rodriguez-Medina et al., 2019) [3], and Trinitarios (Borja Fajardo et al., 2022) [6]. Three key factors were identified to best account for the sample classification: type of variety, functional properties, and quality.

CYP2D6 genotyping and the clinical impact on outcomes in breast cancer tamoxifen-treated patients.

Aims: To report the distribution of allele frequencies of CYP2D6 gene and to evaluate their influence on the clinical outcomes of a group of breast cancer patients receiving adjuvant tamoxifen treatment from Uruguay. Patients & methods: 199 samples were genotyped through real-time polymerase chain reaction assays. Metabolization profiles were inferred from the genotypes. Correlations were evaluated using Pearson's χ2 test. Results: Phenotype frequencies were 0.65 normal (NM), 0.30 intermediate (IM) and 0.05 poor metabolizers (PM). Similar clinical outcomes between NM and (PM + IM) patient groups (odds ratio = 1.011, 95% CI = 0.2703-3.7826; p = 0.987) were found. Conclusion: CYP2D6 allele frequencies were analyzed for the first time in a cohort from Uruguay. Results did not support any impact of CYP2D6 gene polymorphisms on clinical outcomes.

Concordance of the vestibular bone thickness at the level of point a between teleradiography and cone beam computed tomography / Concordancia del espesor óseo vestibular a nivel del punto a entre telerradiografía y tomografía computarizada de haz cónico

Objective: The aim of this study was to determine the concordance of the vestibular bone thickness measured at the level of point A between Teleradiography and Cone Beam Computed Tomography (CBCT). Materials and Methods: This study consisted of a cross-sectional analytical design of concordance that evaluated the teleradiographies and CBCTs of 32 patients. The measurements were performed by three evaluators, specialists in orthodontics. Two of them measured the CBCTs and one evaluated the teleradiographs. The concordance of both tests was determined using the Concordance Correlation Coefficient. Results: When evaluating the value of the vestibular bone thickness at the level of point A between the CBCT and the teleradiography, it was observed that the mean value of the absolute difference between the two was 0.95±0.74, 95%CI [0.68­1.22], being statistically significant (p=0.0027). When the concordance between both tests was analyzed, it was observed that it was poor (CCC=0.204 95%CI [0.014­0.394]), although statistically significant (p<0.00001). Conclusions: It was possible to conclude that there is no concordance in the measurement of the vestibular bone thickness at the level of Point A between the Teleradiography and the CBCT.

Objetivo: El objetivo de este estudio fue determinar la concordancia del espesor óseo vestibular medido a nivel del punto A entre la Telerradiografía y la Tomografía computarizada de haz cónico (CBCT). Materiales y Métodos: Esta investigación presentó un diseño analítico transversal de concordancia en el que se evaluaron las telerradiografías y CBCT de 32 pacientes. Las mediciones fueron realizadas por tres evaluadores especialistas en ortodoncia, dos de ellos midieron los CBCT y uno las telerradiografías. La concordancia de ambos exámenes fue medida mediante Coeficiente de Correlación de Concordancia. Resultados: Al evaluar el valor del grosor óseo vestibular a nivel del punto A entre el CBCT y la telerradiografía, se observó que el valor promedio de diferencia absoluta entre ambos fue de 0,95±0,74 IC95% [0,68­1,22], siendo estadísticamente significativas (p=0,0027). Cuando se analizó la concordancia entre ambos exámenes se observó que esta fue pobre (CCC=0,204 IC95 % [0,014­0,394]), aunque estadísticamente significativa (p<0,00001). Conclusión: Se pudo concluir que no existe concordancia en la medición del espesor óseo vestibular medido a nivel del Punto A entre la Telerradiografía y el CBCT.

Genetic and epigenetic characteristics of patients with colorectal cancer from Uruguay.

Colorectal cancer (CRC), the 3rd most frequent cancer worldwide, affects both men and women. This pathology arises from the progressive accumulation of genetic and epigenetic alterations. In this study, KRAS, NRAS, PIK3CA, and BRAF gene mutations, mismatch repair (MMR) genes methylation profile, microsatellite instability (MSI) and CpG Island Methylator Phenotype (CIMP) status were assessed. The associations of these molecular features with clinicopathological data were also investigated. A hundred and eight unselected CRC samples and their histological and clinical data, were gathered between 2017 and 2020. The prevalence of KRAS, NRAS and BRAF gene mutations was similar to that described in other populations. 28.7% of tumors were KRAS-mutated, mostly in men, distal location, with a CIMP-negative status. BRAFV600E frequency was 6.5% and associated with MSI (p = 0.048), MLH1-methylated (p < 0.001) and CIMP-High (p < 0.001) status. We also confirmed that BRAFV600E tumors were more prevalent in older women and proximal location. A striking different result was the lack of most common variants in the PIK3CA gene. A complete absence of PIK3CA-mutated tumors in a population has not been previously reported. Among MMR genes, the only with an aberrant methylation pattern was MLH1 gene. Its frequency was 9.25%, lower than previously reported. Methylated tumors were most frequent in patients older than 70 years old and proximal tumor location. Finally, CIMP-High status was mainly observed in moderately differentiated tumors with a rate of 15.7%. Our findings were consistent with previous reports in other populations, but also showed some features unique to our cohort. This study is the first to report the analysis of a large number molecular biomarkers of CRC in Uruguay and one of the few performed in Latin-America.

Tamizaje para cáncer de mama con resonancia magnética en mujeres portadoras de mutaciones BRCA y no-BRCA / Magnetic resonance imaging breast cancer screening in women who are carriers of BRCA and non-BRCA gene mutations / Rastreamento de câncer de mama com ressonância magnética em mulheres com mutações BRCA e não BRCA

Introducción: la predisposición hereditaria causada por mutaciones patogénicas de la línea germinal (MPG) explica hasta el 10% de los cánceres de mama. Para reducir su impacto en mujeres mutadas se han propuesto diferentes estrategias, tales como las cirugías reductoras de riesgo o el screening con resonancia magnética (RM) de mamas. Métodos: en este estudio observacional retrospectivo se analizaron los registros de mujeres portadoras de MPG para evaluar las diferentes acciones tomadas luego del test genético. A las pacientes no mastectomizadas se les recomendó ingresar a un programa anual de cribado con RM y se evaluó el porcentaje de adherencia al plan, el número de biopsias efectuadas y el número de cánceres de mama detectados. Resultados: se incluyeron 134 mujeres con MPG, con una distribución en tercios iguales de los genes BRCA1, BRCA2 y genes no-BRCA. Entre las mutadas con indicación de seguimiento, 64% ingresaron al programa de cribado con RM. Las razones que llevaron a las mujeres a no ingresar al programa de seguimiento fueron: la oposición del médico tratante (53%), oposición de la paciente (38%), y falta de recursos (9%). Se realizaron seis biopsias por hallazgos en la RM entre las cuales se detectó un cáncer de mama. La incidencia de cáncer fue de 11 cada 1.000 mujeres-años de riesgo. Conclusiones: nuestro programa de seguimiento con RM de pacientes mutadas logró captar un porcentaje alto de candidatas. Una proporción significativa de las mujeres no ingresó debido la desaprobación del médico tratante o de la propia paciente. La evidencia obtenida revela una necesidad imperiosa de reforzar los programas educativos que destaquen la importancia del seguimiento con RM de las pacientes de alto riesgo en nuestro país.

Summary: Introduction: genetic propensity caused by germline pathogenic mutations explain up to 10% of breast cancer cases. Different strategies have been proposed to reduce its impact on women who are carriers of mutations, such as risk-reducing surgeries or breast magnetic resonance screening. Method: observational, retrospective study analyzing the medical records of women who are carriers of germline pathogenic mutations to assess the different measures taken after the genetic test. Non-mastectomized patients were advised to join an annual MRI screening program and the percentage of adherence to plan was evaluated, along with biopsies performed and the number of breast cancer cases detected. Results: 134 women carriers of germline pathogenic mutations were included in the study, with equal distributions in thirds for BRCA1, BRCA2 and non-BRCA genes. 64% of carriers of mutations who were subject to follow-up checkups joined the RMI screening program. The reasons why women failed to join the follow-up program were: the treating physician objected to the program (53%), the patients opposed to program (38%) and lack of resources (9%). Six biopsies were performed as a consequence of findings in the RMI, and one case of breast cancer was detected. Cancer incidence was 11 out of 1000 women - risk years. Conclusions: our RMI follow-up program for women who are carriers of mutations managed to attract a high percentage of candidates. A significant amount of women failed to join the program because of their treating physician's or their own disapproval. Evidence obtained reveals the dramatic need to reinforce educational programs that emphasize on the importance of RMI follow-up of high risk patients in our country.

Introdução: a predisposição hereditária causada por mutações germinativas patogênicas (GMP) explica até 10% dos cânceres de mama. Para reduzir seu impacto em mulheres com mutações, diferentes estratégias têm sido propostas, como cirurgias de redução de risco ou ressonância magnética (RM) das mamas. Métodos: neste estudo observacional retrospectivo, os registros de mulheres portadoras de MPG foram analisados para avaliar as diferentes ações tomadas após o teste genético. Pacientes não mastectomizadas foram recomendadas a entrar em um programa anual de triagem por ressonância magnética e foram avaliados o percentual de adesão ao plano, o número de biópsias realizadas e o número de cânceres de mama detectados. Resultados: foram incluídas 134 mulheres com MPG, com uma distribuição de terços iguais dos genes BRCA1, BRCA2 e não-BRCA. Entre as mulheres com mutações com indicação de acompanhamento, 64% entraram no programa de triagem por ressonância magnética. Os motivos que levaram as mulheres a não ingressarem ao programa de acompanhamento foram: oposição do médico assistente (53%), oposição da paciente (38%) e falta de recursos (9%). Seis biópsias foram realizadas devido a achados de ressonância magnética, entre os quais foi detectado um câncer de mama. A incidência de câncer foi de 11 por 1.000 mulheres-ano de risco. Conclusões: nosso programa de acompanhamento de ressonância magnética para pacientes com mutação conseguiu capturar uma alta porcentagem de candidatas. Uma proporção significativa de mulheres não entrou devido à falta de aprovação do médico assistente ou da própria paciente. As evidências obtidas revelam a necessidade urgente de reforçar programas educacionais que destaquem a importância do acompanhamento por RM de pacientes de alto risco no Uruguai.

[Effectiveness of high intensity interval training in the cardiorespiratory capacity of people older than 65 years old: A systematic review]. / Efectividad del entrenamiento interválico de alta intensidad en la capacidad cardiorrespiratoria de personas mayores de 65 años, una revisión sistemática.

The objective of this review was to analyze the effect of HIIT on cardiorespiratory fitness, health-related quality of life (HRQL) and safety of its execution in people over 65 years of age. A systematic search was carried out, following the PRISMA recommendations, in 11 electronic databases, evaluating the resolution of the HIIT, affecting cardiorespiratory capacity as the main result and secondarily HRQL and safety of its execution, assessing the risk of bias using Rob 2.0 and ROBINS-I. Thirteen studies met the inclusion criteria, consisting of people >65 years, HIIT intervention >4 weeks, and studies assessing cardiorespiratory fitness. It can be concluded that HIIT improves cardiorespiratory capacity in people over 65 years of age, with respect to HRQL and the safety of its execution, the results are not conclusive. It becomes essential to unify criteria in intervention protocols, requiring further research in this regard.

Aggressive mutation in a familial adenomatous polyposis syndrome family: when phenotype guides clinical surveillance.

Familial adenomatous polyposis (FAP) is an autosomal dominant genetic condition, caused by mutations in the adenomatous polyposis coli (APC) tumor suppressor gene. Desmoid tumors (DTs) are seen in 15% to 20% of FAP patients. Specific location of mutation serves as a guide to predict colonic and extra colonic manifestations and their aggressiveness. A severe FAP-phenotypic family was registered in a genetic counselling high-risk Uruguayan hereditary cancer clinic. Proband's DNA was analysed by NGS, detecting a pathogenic mutation in APC gene. All willing family members were counselled and encouraged to be tested. Here we report a kindred formed by 16 individuals with a very severe FAP phenotype. A two-base deletion mutation: c.4393_4394delAG in APC gene and a consequent premature stop codon was detected. DTs were diagnosed in 6 individuals, ranging from 2 to 25 years of age. The causes of death were diverse: gastric cancer, rectal cancer and desmoid tumor. The already described genotype-phenotype correlation has proved its worth in this family, as clinical features reflect the mutation location at 3' end of APC gene. The inheritable and lethal nature of the disease needs a tailored follow up approach in order to reduce mortality, optimize local tumor control, and preserve patients' quality of life.

Cell Saver en Obstetricia. Primer caso reportado en Chile / Cell Saver in Obstetrics. First case reported in Chile

La hemorragia obstétrica representa un desafío para el equipo médico, aportando con importante morbilidad y mortalidad a las pacientes embarazadas.El manejo adecuado, precoz y expedito beneficia el logro de resultados favorables para la madre e hijo; es por esto que debemos reconocer activamente aquellas pacientes en riesgo de presentar un sangrado obstétrico significativo.Presentamos el primer caso reportado en Chile de una paciente embarazada con mala inserción placentaria, sometida a cesárea y decómo se realizó el manejo del sangrado intraoperatorio, con énfasis en el uso de Cell Saver como técnica ahorradora de sangre.(AU)

Obstetric hemorrhage is a challenge for the medical team, contributing with significant morbidity and mortality to the pregnant patient.An appropriate, early, and expeditious management eases the achievement of favourable results for mother and son. We must recognizeactively those patients at risk of a significant obstetric bleeding.We present the first case report in Chile of a pregnant patient with abnormal placentation, undergoing a caesarean section and howbleeding was handled during the surgery, with emphasis on the use of a Cell Saver device as a blood-saving technique.(AU)

[Tuberculous abscess of the thoracic wall secondary to nodular tuberculosis: Atypical presentation in an older adult]. / Absceso tuberculoso de pared torácica secundario a tuberculosis ganglionar; presentación atípica.

Tuberculosis (tb) of the chest wall is uncommon and it represents less than 5% of all cases of musculoskeletal tb and only 1-2% of tb. We present the case of an elderly woman with tb of the chest wall secondary to a nodal tb with an unusual presentation. The diagnosis of this entity is difficult because the disease often mimics other diseases such as pyogenic abscess, chest wall.

Effects of local land-use on riparian vegetation, water quality, and the functional organization of macroinvertebrate assemblages.

Land-use change is a principal factor affecting riparian vegetation and river biodiversity. In Chile, land-use change has drastically intensified over the last decade, with native forests converted to exotic forest plantations and agricultural land. However, the effects thereof on aquatic ecosystems are not well understood. Closing this knowledge gap first requires understanding how human perturbations affect riparian and stream biota. Identified biological indicators could then be applied to determine the health of fluvial ecosystems. Therefore, this study investigated the effects of land-use change on the health of riparian and aquatic ecosystems by assessing riparian vegetation, water quality, benthic macroinvertebrate assemblages, and functional feeding groups. Twenty-one sites in catchment areas with different land-uses (i.e. pristine forests, native forests, exotic forest plantations, and agricultural land) were selected and sampled during the 2010 to 2012 dry seasons. Riparian vegetation quality was highest in pristine forests. Per the modified Macroinvertebrate Family Biotic Index for Chilean species, the best conditions existed in native forests and the worst in agricultural catchments. Water quality and macroinvertebrate assemblages significantly varied across land-use areas, with forest plantations and agricultural land having high nutrient concentrations, conductivity, suspended solids, and apparent color. Macroinvertebrate assemblage diversity was lowest for agricultural and exotic forest plantation catchments, with notable non-insect representation. Collector-gatherers were the most abundant functional feeding group, suggesting importance independent of land-use. Land-use areas showed no significant differences in functional feeding groups. In conclusion, anthropogenic land-use changes were detectable through riparian quality, water quality, and macroinvertebrate assemblages, but not through functional feeding groups. These data, particularly the riparian vegetation and macroinvertebrate assemblage parameters, could be applied towards the conservation and management of riparian ecosystems through land-use change studies.

Cáncer de mama y ovario hereditario en Uruguay: resultados del screening para mutaciones en genes de susceptibilidad por secuenciación de nueva generación / Hereditary breast and ovary cancer in Uruguay: screening results for mutations in susceptibility genes by new generation sequencing

Introducción: el cáncer de mama representa la primera causa de muerte por cáncer en mujeres de Uruguay. Se estima que cerca de 7% son causados por mutaciones en el ácido desoxirribonucleico germinal. Los costos de la secuenciación genética han descendido dramáticamente gracias a la aparición de la secuenciación de nueva generación (NGS). El cambio tecnológico abrió una nueva etapa en el estudio del cáncer hereditario en nuestro país. Objetivo: comunicar los resultados de la utilización de tecnología NGS y paneles multigénicos en familias uruguayas con alto riesgo de cáncer de mama hereditario. Pacientes y método: se secuenciaron 135 familias de alto riesgo que provenían de la consulta de consejería genética que funciona en el Grupo Colaborativo Uruguayo: Investigación de afecciones oncológicas hereditarias. Cuando la historia familiar sugería claramente un síndrome de cáncer de mama y ovario hereditario se efectuó secuenciación NGS exclusiva de los genes BRCA1 y 2; cuando el patrón familiar no configuraba claramente se utilizó un panel multigénico. Resultados: se efectuó NGS exclusiva de genes BRCA1 y 2 en 62 familias y un panel multigénico en 73 familias. Se identificaron en total 29 mutaciones patógenas (21 en genes BRCA y 8 en otros genes). Dos de ellas fueron noveles y tres pueden considerarse recurrentes en la población uruguaya. Conclusiones: este trabajo es el primero en Uruguay en reportar el resultado de esta nueva tecnología en el cáncer de mama hereditario. El hallazgo de 29 mutaciones patógenas nos ayuda a delinear el perfil mutacional de nuestro país.

Introduction: breast cancer is women's first cause of death in Uruguay. According to estimations, around 7% of cases result from germinal mutations by deoxyribonucleic acid. The cost of genetic sequencing has dramatically dropped thanks to the arrival of next-generation sequencing (NGS). This technological change opened a new era in the study of hereditary cancer in our country. Objective:to communicate the results of using NGS technology and multigenic panels in Uruguayan families with high risk of hereditary breast cancer. Method: 135 high risk families referred by the genetic counselling consultation that is provided at the Uruguayan Collaborative Group (Hereditary Oncological Conditions Research) were sequenced. When the family history clearly suggested hereditary breast and ovary cancer was a possibility, exclusive NGS sequencing was done for BRCA1 and BRCA2 genes; when the family pattern was not clear to this respect, multigenic panels were used. Results: exclusive NGS sequencing for BRCA1 and BRCA2 genes was done in 62 families, and multigenic panels were used in 73 families. 29 pathogenic mutations were identified (21 in BRCA genes and 8 in other genes). Two of them were new to the disease and three could be considered recurrent in the Uruguayan population. Conclusions:this study is the first one in Uruguay to report the results of this new technology in hereditary breast cancer. The finding of 29 pathogenic mutations contributes to outlining the mutational profile of our country.

Introdução: o câncer de mama é a primeira causa de morte por câncer em mulheres no Uruguai. Estima-se que aproximadamente 7% sejam causados por mutações no ácido desoxirribonucleico germinal. Os custos da sequenciação genética diminuíram dramaticamente graças ao aparecimento da sequenciação de nova geração (NGS). Esta nova tecnologia deu inicio a uma nueva etapa no estudo do câncer hereditário no nosso país. Objetivo: comunicar os resultados da utilização de tecnologia NGS e painéis mutagênicos em famílias uruguaias com alto risco de câncer de mama hereditário. Pacientes e método: 135 famílias de alto risco originárias do aconselhamento genético que funciona no Grupo Colaborativo Uruguaio: Pesquisa de afecções oncológicas hereditárias foram sequenciadas. Quando a história familiar sugeria uma síndrome de câncer de mama e ovário hereditários fez-se a secuenciacao NGS exclusivamente dos genes BRCA1 e 2; quando o padrão familiar não era claro foi utilizado um painel multigênico. Resultados: realizou-se NGS exclusivamente de genes BRCA1 e 2 em 62 famílias e um painel multigênico em 73 famílias. Foram identificadas 29 mutações patogênicas (21 em genes BRCA e 8 em outros genes). Duas eram novas e três podem ser consideradas como recorrentes na população uruguaia. Conclusões: este trabalho é o primeiro que apresenta os resultados desta nova tecnologia aplicada ao câncer de mama hereditário no Uruguai. O achado de 29 mutações patogênicas ajuda a definir o perfil mutacional do nosso país.

The effect of a mindfulness-based intervention in cognitive functions and psychological well-being applied as an early intervention in schizophrenia and high-risk mental state in a Chilean sample: study protocol for a randomized controlled trial.

BACKGROUND: According to the projections of the World Health Organization, 15% of all disabilities will be associated with mental illnesses by 2020. One of the mental disorders with the largest social impacts due to high personal and family costs is psychosis. Among the most effective psychological approaches to treat schizophrenia and other psychotic disorders at the world level is cognitive behavioral therapy. Recently, cognitive behavioral therapy has introduced several tools and strategies that promote psychological processes based on acceptance and mindfulness. A large number of studies support the effectiveness of mindfulness in dealing with various mental health problems, including psychosis. This study is aimed at determining the efficiency of a mindfulness-based program in increasing cognitive function and psychological well-being in patients with a first episode of schizophrenia and a high risk mental state (those at risk of developing an episode of psychosis). METHODS AND DESIGN: This is an experimentally designed, multi-center randomized controlled trial, with a 3-month follow-up period. The study participants will be 48 patients diagnosed with schizophrenia (first episode) and 48 with a high-risk mental state, from Santiago, Chile, aged between 15 and 35 years. Participants will be submitted to a mindfulness-based intervention (MBI), which will involve taking part in eight mindfulness workshops adapted for people with psychosis. Workshops will last approximately 1.5 hours and take place once a week, over 8 weeks. The primary outcome will be the cognitive function through Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) and the secondary outcome will be psychological well-being measured by self-reporting questionnaires. DISCUSSION: The outcomes of this trial will add empirical evidence to the benefits and feasibility of MBIs for the psychotherapeutic treatment of patients with schizophrenia and high-risk mental states in reducing cognitive impairment in attention, working memory, and social cognition, as well as increasing the psychological well-being by empowering the patients' personal resources in the management of their own symptoms and psychotic experiences. TRIAL REGISTRATION: ISRCTN registration number ISRCTN24327446 . Registered on 12 September 2016.

Sonic versus ultrasonic activation for the cleaning of the root canal after post space preparation: an in vitro study / Activación sónica versus ultrasónica en la limpieza del canal radicular posterior a la preparación del lecho para poste: estudio in vitro

To compare the efficacy of 3 intracanal cleaning protocols used before cementation of prosthetic posts. Material and Methods: 40 anterior teeth received endodontic treatment in hand, using lateral condensation technique. After two weeks, gutta-percha was appropriately removed from the teeth to get the necessary space to install a post. Then, teeth were randomly divided into groups; root surface was treated with chlorhexidine (CHX) activated by ultrasound (US) (group I), with chlorhexidine activated by sonic instrumentation (S) (group II), chlorhexidine without activation (group III) and without treatment (group IV). All teeth were fractured longitudinally getting 2 sections. The middle third of the root canal was microphotographed with a scanning electron microscope (SEM) and the contaminated surface was measured using detritus with ImageJ 1.47. It was analyzed with Kruskal-Wallis-test using GraphPad Prism 5.01. Results: The median percentage of contaminated area of Group I was 20.06 percent, Group II, 19.3 percent; Group III, 36.05 percent; and Group IV, 56.45 percent. Conclusion: There are significant differences among different intracanal cleaning protocols in the removal efficiency of detritus from the root canal, being the activated protocols the most effective ones...

Comparar la eficacia de 3 protocolos de limpieza intraconducto utilizados previo a la cementación de pernos protésicos. Materiales y métodos: A 40 dientes anteriores se les realizó un tratamiento endodóntico en mano, mediante la técnica de condensación lateral. Luego de dos semanas, se realizó la desobturación de los mismos retirando la cantidad de gutapercha que otorgue el espacio necesario para alojar un poste. A continuación, los dientes fueron divididos aleatoriamente en grupos; superficie radicular tratada con clorhexidina (CHX) activado con ultrasonido (US) (grupo I), con clorhexidina activado con instrumental sónico (S) (grupo II), con clorhexidina sin activación (grupo III) y sin ningún tratamiento (grupo IV). Todos los dientes se fracturaron longitudinalmente obteniendo 2 secciones. Se microfotografió el tercio medio del conducto radicular con microscopio electrónico de barrido (MEB) y se midió la superficie contaminada con detritus usando ImageJ 1.47. Se analizó con test Kruskall-Wallis usando GraphPad-Prism 5.01. Resultados: La mediana del porcentaje de área contaminada del Grupo I fue 20,06 por ciento, Grupo II de 19,3 por ciento, Grupo III de 36,05 por ciento y Grupo IV de 56,45 por ciento. Conclusión: Existen diferencias significativas entre los distintos protocolos de limpieza intraconducto en la eficacia de eliminación de detritus del conducto radicular, siendo los protocolos activados más eficientes...

History of dental caries and need for dental treatment among children with visual disabilities, Chile, 2014 / Historia de caries y necesidad de tratamiento odontológico en niños con discapacidad visual, Chile, 2014

Objective: To determine the history of dental caries and the need for dental treatment among visually disabled children between 6 and 12 years old attending special Schools in Chile during 2014. Material and method: Descriptive and observational cross-sectional study. The target population was children with visual disabilities from special schools in Chile. A clinical examination was performed to obtain a dmft/DMFT index and know the need for treatment. Two calibrated examiners performed the visual clinical examination, according to the WHO criteria. Additionally, clinically detectable enamel lesions on cavitated and non cavitated surfaces were recorded. The recorded data were analyzed using descriptive statistics. Results: Ninety-four children attending to seven special schools were examined. The average dmft index was 2.05 (SD+/-2.6) and DMFT index was 0.96 (SD+/-1.3). For 6 year olds, the dmft/DMFT index was 3/0, respectively. For 12 year olds, the dmft index was 0.17 and the DMFT index was 1.42. Caries prevalence was 56.3 percent From the total, 95 percent of children needed sealants, 50 percent needed at least one restoration of one face, and 29 percent restoration of 2 or more surfaces. As for enamel lesions, 34 percent of children had at least one non cavitated lesion and 48 percent of them had a cavitated lesion. Conclusion: History of dental caries is similar to that reported in the general population; however, there is a high need for preventive treatment. Therefore, it is necessary to conduct more research to clarify whether these patients have a higher cariogenic risk due to their visual impairment in order to establish effective promotional/ preventive strategies.

Objetivo: Determinar la historia de caries y la necesidad de tratamiento odontológico de niños con discapacidad visual entre 6-12 años, pertenecientes a Escuelas Especiales de Chile, el año 2014. Material y método: Estudio observacional descriptivo de corte transversal. Se examina-ron niños con discapacidad visual de escuelas especiales en Chile, según los criterios de la OMS, para obtener el ceod, COPD y la necesidad de tratamiento. Se registraron las lesiones de esmalte clínicamente detectables en superficies cavitadas y no cavitadas. Los resultados se analizaron mediante estadística descriptiva. Resultados: 94 niños fueron examinados, pertenecientes a 7 escuelas especiales. El ceod promedio fue 2,05 (SD +/- 2,6) y el COPD fue de 0,96 (SD +/- 1,3). A los 6 años el ceod/COPD fue de 3/0 respectivamente. A los 12 años el ceod fue 0,17 y COPD fue 1,42. La prevalencia de caries fue de 56,3 por ciento. El 95 por ciento necesita sellantes, el 50 por ciento necesita a lo menos una restauración de una cara, y el 29 por ciento una restauración de 2 o más superficies. En cuanto a las lesiones de esmalte, el 34 por ciento de los niños presentaban al menos una lesión no cavitada y un 48 por ciento de ellos tenían una lesión cavitada. Conclusión: La historia de caries es similar a lo descrito en la población general, sin embargo, existe una alta necesidad de tratamiento preventivo. Es necesario realizar más investigaciones que permitan esclarecer si poseen un mayor riesgo cariogénico debido a su alteración visual, para así establecer estrategias promocionales/preventivas efectivas.

Prevalencia de edentulismo parcial y total en islas butachauques y Tac, Quemchi, 2013 / Prevalence of partial and total edentulism in butachauquesand tac islands, Quemchi, (Chile) 2013

Objetivo: Determinar la prevalencia de edentulismo y clasificación topográ fica de Kennedy en población mayor de 20 años de islas Butachauques y Tac. Material y Método: Estudio observacional descriptivo. Se incluyeron sujetos mayores de 20 años en los servicios de salud de islas Butachauques y Tac durante los meses de agosto y septiembre de 2013. Se solicitó a cada sujeto de investigación la firma de un consentimiento informado. La información recopilada fue ingresada a una planilla de registro de datos diseñada para la investigación. Las variables utilizadas fueron género, edad, cantidad de dientes presentes, portador de prótesis y tipo de edentulismo según clasificación topográfica de Kennedy, para luego realizarse un análisis de estadística descriptiva mediante SPSS 18 (IBM® SPSS® software). Resultados: Fueron evaluados 88 sujetos, de los cuales un 39.7 por ciento hombres y un 60.3 por ciento mujeres. Un 89.7 por ciento de los sujetos presentó ausencia de al menos uno de sus dientes y del total de éstos, el 29.4 por ciento utilizaba prótesis dentales. La clasificación de Kennedy maxilar más prevalente fue Clase 3 y en la arcada Mandibular fueron similares resultados para Clase 1 y 3. Conclusión: Estos resultados demuestran la alta prevalencia de sujetos edéntulos en los distintos servicios de salud de las islas y la necesidad de realizar programas de promoción y prevención para minimizar el daño en la salud de las personas de estas localidades.

Aim: To determine the prevalence of edentulism and Kennedy topographic classification in the population aged over 20 years of the Butachauques and Tac islands, Quemchi, Chile. Materials and Methods: Descriptive study. We included subjects aged over 20 years at health services of the Butachauques and Tac islands during the months of August and September 2013. Subjects were asked to sign an informed consent form. The information collected was entered into a data entry form. The variables used were gender, age, number of teeth present, and type of prosthesis and edentulous type according to Kennedy's topographic classification. Descriptive statistics were determined using SPSS 18 (IBM® SPSS® software) statistical software. Results: 88 subjects, 39.7 percent men and 60.3 percent women, were evaluated. 89.7 percent of the subjects had absence of at least one of their teeth and 29.4 percent of these were using dentures. The most prevalent maxillary classification was Class 3, while the mandibular arch had similar results for class 1 and 3. Conclusion: These results demonstrate a high prevalence of edentulous subjects in the various health services of the islands, and the need for promotion and prevention programs to minimize damage to the health of people in these localities.