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1.
Genes (Basel) ; 11(8)2020 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-32824045

RESUMO

Analysis of ancient and historical DNA has great potential to trace the genetic diversity of local cattle populations during their centuries-long development. Forty-nine specimens representing five cattle breeds (Kholmogor, Yaroslavl, Great Russian, Novgorod, and Holland), dated from the end of the 19th century to the first half of the 20th century, were genotyped for nine polymorphic microsatellite loci. Using a multiple-tube approach, we determined the consensus genotypes of all samples/loci analysed. Amplification errors, including allelic drop-out (ADO) and false alleles (FA), occurred with an average frequency of 2.35% and 0.79%, respectively. A significant effect of allelic length on ADO rate (r2 = 0.620, p = 0.05) was shown. We did not observe significant differences in genetic diversity among historical samples and modern representatives of Kholmogor and Yaroslavl breeds. The unbiased expected heterozygosity values were 0.726-0.774 and 0.708-0.739; the allelic richness values were 2.716-2.893 and 2.661-2.758 for the historical and modern samples, respectively. Analyses of FST and Jost's D genetic distances, and the results of STRUCTURE clustering, showed the maintenance of a part of historical components in the modern populations of Kholmogor and Yaroslavl cattle. Our study contributes to the conservation of biodiversity in the local Russian genetic resources of cattle.


Assuntos
Alelos , Variação Genética , Genética Populacional , Repetições de Microssatélites , Animais , Cruzamento , Bovinos , Genótipo , Análise de Componente Principal
2.
Arch Anim Breed ; 63(2): 409-416, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33473365

RESUMO

The use of specialized meat breeds in cattle breeding programs is considered very promising for improving herds' productivity. However, in animal genotype, along with genes that positively affect the productivity signs, there are genes whose mutations, known as genetic defects, negatively affect the health of animals. The aim of the study was the screening of the Russian populations of Aberdeen Angus, Hereford and Belgian Blue cattle on gene mutations associated with the genetic defects of arthrogryposis multiplex (AM), osteopetrosis (OS), developmental duplication (DD), double muscling (M1), hypotrichosis (HY) and maple syrup urine disease (MSUD) as well as the F94L polymorphism of myostatin gene (MSTN) linked with the gene responsible for less fat content in the carcass by means of DNA analysis. In the article, test systems based on the polymerase chain reaction method are presented. The analysis of the Aberdeen Angus ( n = 4480 ) population has revealed 0.19  ±  0.09 % animal M1 carriers, 0.53  ±  0.03 % OS carriers, 1.92  ±  0.09 % AM carriers and 9.00  ±  0.20 % DD carriers. The genotyping of Hereford cattle of Russian populations ( n = 525 ) has not revealed any individual carriers of MSUD or HY genetic defects. All of the Belgian Blue population ( n = 92 ) animals were heterozygous M1 carriers. The study of the F94L MSTN polymorphism has demonstrated extremely high frequencies of the desirable A allele (0.93 and 0.90) in two Aberdeen Angus populations with an average mean of 0.63  ±  0.08, which was 32 % higher compared to the Belgian Blue population. The results suggest the high genetic potential of the Aberdeen Angus and Belgian Blue cattle, but the existence in the genotypes of the mutant alleles associated with hereditary diseases indicates the risk of uncontrolled use of these breeds.

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