A dataset of four probiotic Bifidobacterium strains genome assemblies.

A dataset of four draft genome sequences of Bifidobacterium strains is presented. All four genome assemblies are high-quality drafts characterized by high completeness and low contamination levels. GC content of the genomes varied in the range between 59.27% and 62.77%. Genome sequences were annotated for further functional and taxonomical analyses of the respective Bifidobacterium strains. Genetic determinants of probiotic capabilities, including the genes, related to utilization of human milk oligosaccharides and mucin, as well as the genes, encoding bile salt hydrolase were identified. The genome of B. bifidum VKPM=Ac-1784 has been shown to possess two bacteriocin gene clusters. The dataset expands knowledge on genomic diversity of probiotic strains of Bifidobacterium genus. The dataset is available under PRJNA656137 accession number in NCBI database and under zyv26t6x5r accession number in Mendeley Data repository.

Conformational changes in inter-α-trypsin inhibitor heavy chain 4 activate its tumor-specific activity in mice with B16 melanoma.

It is known that blood serum proteins of tumor­bearing mice display tumor­specific activity. However, to date, the nature of this activity has remained elusive, and no tumor­specific proteins have been detected in the blood serum of tumor­bearing animals compared with those in healthy animals. The present study postulated and investigated the hypothesis that the observed tumor­specific activity of the blood serum proteins is not associated with the appearance of novel serum proteins but with changes in the conformation of the existing ones. The present study showed conformational changes of two serum albumin proteins and inter­α­trypsin inhibitor heavy chain 4 (ITIH4) in mice with B16 melanoma compared to tumor­free mice, as determined by differences in the products of proteolysis by proteomic analysis following column chromatography. The differences in the conformation of serum albumin in mice with B16 melanoma and tumor­free mice were accompanied by a change in the interaction of these molecules with the fatty acid spin probe 16­doxyl stearic acid. The differential conformation of ITIH4 in mice with B16 melanoma and that in tumor­free mice was accompanied by inhibition of tumor growth and increased life span. Analysis of the role of protease­anti­proteases (serpins) in the serum of tumor­bearing animals in tumor growth confirmed the hypothesis that tumor growth in the body is mediated, at least in part, via balancing of serpins.