Accurate assessment of parabens exposure in healthy Chinese female adults: Findings from a multi-pathway exposure assessment coupled with intervention study.

Exposure of humans to parabens is widespread and urinary parabens are widely used as exposure biomarkers. However, are the levels of these chemicals suitable to assess exposure to parabens? We conducted an intervention study by controlling the use of personal care products (PCPs) to explore the exposure of parabens. Ten female participants were recruited who were treated with different types of PCPs during the 18-day study period. The concentrations of parabens and their metabolites in matrices of different exposure pathways (dust, drinking water and dietary food) and urine samples were determined. We demonstrated that PCPs were the major sources of parabens, accounting for >99% of total exposure. The metabolites were nonspecific to individual parabens and could not be used as exposure biomarkers. Urinary paraben concentrations were positively correlated with external exposure levels. However, poor reproducibility was observed, with intraclass correlation coefficients (ICC) ranging from 0.125 to 0.295 in unadjusted urinary concentrations. Creatinine-adjusting could not significantly improve the ICC values in random spot samples. After adjusting for both creatinine and kinetic models, the ICC values ranged from 0.695 to 0.886, indicating a good reproducibility. So, toxicokinetic parameters may be taken into consideration for precise monitoring of exposures for the non-persistent pollutants.

Structure-Directed Screening and Analysis of Thyroid-Disrupting Chemicals Targeting Transthyretin Based on Molecular Recognition and Chromatographic Separation.

Exposure to thyroid-disrupting chemicals (TDCs) poses a great threat to human health. However, the screening and analysis of TDCs in environmental samples remain a tough work. In this study, we reported a structure-directed strategy for analyzing TDCs targeting transthyretin (TTR) based on molecular imprinting and chromatographic separation. The imprinted composites were prepared using l-thyroxine (T4) as a template and a tryptophan-like monomer screened from the amino acid library. The imprinted composites exhibited an adsorption capacity of 22.2 µmol g-1 for T4 and an imprinting factor of 2.1. Chromatographic testing was then conducted among 72 chemicals using the imprinted composites-packed column. High retention factors were observed for chemicals that were structurally similar to T4. The chromatographic results were compared with a data set of 45 chemicals with known activities toward TTR. The results suggested that chemicals can be distinguished as TTR binders and nonbinders by retention factors, with a predictive accuracy of more than 90%. Moreover, the retention factors of chemicals were highly correlated with the reported relative potencies obtained from TTR assays. Thus, screening of TTR-binding chemicals can be realized through this simple chromatographic method. The imprinted composites were applied for target analysis and nontarget analysis of TTR-binding chemicals in dust samples. Three new TTR binders were successfully identified and verified by this method. The combination of molecular imprinting and chromatography opens up a new approach for screening TDCs targeting TTR.

Coiled molecularly imprinted polymer layer open-tubular capillary tube for detection of parabens in personal care and cosmetic products.

Personal care and cosmetic products (PCPs) are the primary exposure pathway of humans to parabens and their safety has become a public concern. However, sample pretreatment of PCPs is a great challenge due to their complexities and diversity. In this study, epoxide modified molecularly imprinted polymers (MIPs) were synthesized using ethylparaben as a template, methacrylic acid and isobutyl vinyl ether as co-monomers and glycidilmethacrylate as a post-modified monomer. MIP layer open-tubular tubes were prepared by modifying branched polyethylenimine and then grafting MIPs onto the inner surface of Teflon capillary tubes. The tube was coiled to effectively increase mass transfer and coupled to an HPLC-UV system for parabens detection in PCPs. Matrix interference was significantly decreased while efficient enrichment and recoveries were obtained. Under optimized conditions, the linear range for parabens detection was 0.5-600 ng mL-1 with detection limits of 0.2 to 0.3 ng mL-1. The system was used to study the contents of parabens in popular PCPs. The concentrations of parabens in 108 PCPs ranged from <0.5 ng g-1 to 2856 µg g-1 with geometric mean of 250.3 µg g-1. Almost all of the products contained at least one kind of parabens; methyl paraben (geometric mean: 182.9 µg g-1) and n-propyl paraben (geometric mean: 42.5 µg g-1) were the predominant compounds had been found in the samples. This method could be useful for human exposure assessment towards parabens.