Occurrence and correlation of microplastics and dibutyl phthalate in rivers from Pearl River Delta, China.

Microplastics have been identified as the novel contaminants in various environments. Phthalates would be released from plasticized microplastics into a riverine environment while transporting to a marine region, but data on their relationship in rivers have been scarce. In this study, the occurrence, distribution and correlation of microplastics and dibutyl phthalate (DBP) in two rivers from the Pearl River Estuary were investigated. The elevated level of DBP in the Qianshan River (2.70 ± 0.20 µg/L) was in alignment with the presence of highest microplastic concentration at the same sampling site (15.8 ± 9.8 items/L). A positive correlation was observed between microplastics and DBP in all sampling sites (p < 0.05). The results showed that UV irradiation from sunlight was a majorly inducing factor of DBP leaching from polyethylene microplastics. The concentrations of chemical additives in some degrees reflect the microplastic pollution, but environmental factors and multidimensionality of microplastics such as residence times and types may cause spatial differences of chemical additives in aquatic systems.

Overexpression of TaMYB4 Confers Freezing Tolerance in Arabidopsis thaliana.

Freezing stress is one of the main factors limiting the growth and yield of wheat. In this study, we found that TaMYB4 expression was significantly upregulated in the tillering nodes of the strong cold-resistant winter wheat variety Dongnongdongmai1 (Dn1) under freezing stress. Weighted gene co-expression network analysis, qRT-PCR and protein-DNA interaction experiments demonstrated that monodehydroascorbate reductase (TaMDHAR) is a direct target of TaMYB4. The results showed that overexpression of TaMYB4 enhanced the freezing tolerance of transgenic Arabidopsis. In TaMYB4 overexpression lines (OE-TaMYB4), AtMDHAR2 expression was upregulated and ascorbate-glutathione (AsA-GSH) cycle operation was enhanced. In addition, the expression of cold stress marker genes such as AtCBF1, AtCBF2, AtCBF3, AtCOR15A, AtCOR47, AtKIN1 and AtRD29A in OE-TaMYB4 lines was significantly upregulated. Therefore, TaMYB4 may increase freezing tolerance as a transcription factor (TF) in Arabidopsis through the AsA-GSH cycle and DREB/CBF signaling pathway. This study provides a potential gene for molecular breeding against freezing stress.

The physiological dissimilarities of Holstein dairy cows with different milk yields.

BACKGROUND: Even if breed, parity, dietary and environmental management are same, dairy cows still have notable differences in milk yield that may be underpinned by physiologic differences. OBJECTIVES: This study aimed to investigate the physiological dissimilarities of dairy cows with different milk yields. METHODS: Thirty cows were sorted into high milk-yielding cows (group H: 58.93±2.31 kg/day), moderate milk-yielding cows (group M: 44.99±0.54 kg/day), and low milk-yielding cows (group L: 24.99±6.83 kg/day) according to milk yield. Blood was collected and serum parameters were assessed. Rumen fluid was collected for the evaluation of rumen fermentation parameters (RFPs) and bacterial community composition (BCC). RESULTS: Serum prolactin, growth hormone, glutathione peroxidase, immunoglobulin A and non-esterified fatty acid had a significantly positive correlation with milk yield (p < 0.05), whereas serum glucagon and total antioxidant capacity had a significantly negative correlation with milk yield (p < 0.05). The concentration of valeric acid and the ratio of acetic acid to propionic acid in the rumen fluid in group H was significantly lower than that in group L (p < 0.05). The concentration of acetic acid and butyric acid in group H was significantly lower than that in groups M and L (p < 0.05). The relative abundances of Ruminococcaceae_NK4A214_group, Prevotella_1, Rikenellaceae_RC9_gut_group, Christensenellaceae_R-7_group, Muribaculaceae, and Ruminococcus_2 were negatively correlated with milk yield, whereas the relative abundance of Succinivibrionaceae_UCG-001, Lachnospiraceae_NK3A20_group, Shuttleworthia and Dialister were positively correlated with milk yield (p < 0.05). CONCLUSIONS: This study indicates that dairy cows with different milk yields have clear divergence in serum indicators, RFPs, BCC and rumen microbial metabolism.

Reconstructing the dynamical quantum phase transitions via dimensional expansion in a generalized Su-Schrieffer-Heeger model.

We know that a one-dimensional (1D) modulated system can simulate 2D topological states by expanding the dimension. This scenario provides a justifiable avenue to test the dilatation of the dynamical quantum phase transition (DQPT). Through a generalized Su-Schrieffer-Heeger model, we have shown how the Loschmidt echo, Fisher zero, and Dynamical topological order parameter (DTOP) transit from one to two dimensions. Owing to the introduced pseudomomentum, the derivative of the return rate does not always capture the DQPT well, but the Fisher zero and the DTOP can be treated as faithful indicators. A topology-independent parameter will also affect the occurrence of the DQPTs for quenches inside a given phase. Moreover, a comparison with the Haldane model owning the same phase diagram implies that a pair of fixed points will lead to different critical momentum distributions, thus different robustness, further reminding us that the correspondences between the equilibrium and dynamical phases transitions are multifarious.

Protective Mechanism of Leucine and Isoleucine against H2O2-Induced Oxidative Damage in Bovine Mammary Epithelial Cells.

Leucine and isoleucine possess antioxidative and anti-inflammatory properties. However, their underlying protective mechanisms against oxidative damage remain unknown. Therefore, in this study, the protective mechanism of leucine and isoleucine against H2O2-induced oxidative damage in a bovine mammary epithelial cell lines (MAC-T cells) were investigated. Briefly, MAC-T cells exposed or free to H2O2 were incubated with different combinations of leucine and isoleucine. The cellular relative proliferation rate and viability, oxidative stress indicators, and inflammatory factors were determined by specific commercial kits. The genes related to barrier functions was measured by real-time quantitative PCR. The protein expression differences were explored by 4D label-free quantitative proteomic analyses and validated by parallel reaction monitoring. The results revealed that leucine and isoleucine increased cell proliferation, total antioxidant status (TAS), and the relative mRNA expression of occludin, as well as decreased malondialdehyde (MDA), total oxidant status (TOS)/TAS, IL-6, IL-1ß, and TOS. When leucine and isoleucine were combined, MDA, TOS/TAS, and the relative mRNA expression levels of claudin-1, occludin, and zonula occludens-1 increased when compared to leucine or isoleucine alone. Proteomics analyses revealed that leucine significantly upregulated the propanoate metabolism; valine, leucine, and isoleucine degradation; and thermogenesis pathways, whereas isoleucine significantly upregulated the peroxisome and propanoate metabolism pathways. In conclusion, leucine protected MAC-T cells from H2O2-induced oxidative stress by generating more ATP to supplement energy demands, and isoleucine improved the deficit in peroxisome transport and promoted acetyl-CoA production. The findings of this study enhance our understanding of the protective mechanisms of leucine and isoleucine against oxidative damage.

Genome-Wide Identification and Analysis of bZIP Gene Family and Resistance of TaABI5 (TabZIP96) under Freezing Stress in Wheat (Triticum aestivum).

The basic leucine zipper (bZIP) regulates plant growth and responds to stress as a key transcription factor of the Abscisic acid (ABA) signaling pathway. In this study, TabZIP genes were identified in wheat and the gene structure, physicochemical properties, cis-acting elements, and gene collinearity were analyzed. RNA-Seq and qRT-PCR analysis showed that ABA and abiotic stress induced most TabZIP genes expression. The ectopic expression of TaABI5 up-regulated the expression of several cold-responsive genes in Arabidopsis. Physiological indexes of seedlings of different lines under freezing stress showed that TaABI5 enhanced the freezing tolerance of plants. Subcellular localization showed that TaABI5 is localized in the nucleus. Furthermore, TaABI5 physically interacted with cold-resistant transcription factor TaICE1 in yeast two-hybrid system. In conclusion, this study identified and analyzed members of the TabZIP gene family in wheat. It proved for the first time that the gene TaABI5 affected the cold tolerance of transgenic plants and was convenient for us to understand the cold resistance molecular mechanism of TaABI5. These results will provide a new inspiration for further study on improving plant abiotic stress resistance.

Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase genes of winter wheat enhance the cold tolerance of transgenic Arabidopsis.

In this study, winter wheat G6PDH (TaG6PDH) and 6PGDH (Ta6PGDH) were investigated. Both their expression and their activity were upregulated under cold stress, suggesting that TaG6PDH and Ta6PGDH positively respond to cold stress in winter wheat. Exogenous abscisic acid (ABA) treatment markedly increased the expression and activity levels of TaG6PDH and Ta6PGDH in winter wheat under cold stress. Subsequently, TaG6PDH-and Ta6PGDH were overexpressed in Arabidopsis, and showed stronger reactive oxygen species (ROS)-scavenging ability and higher survival rate compared with wild-type (WT) plants under cold stress. In addition, we found that TaG6PDH and Ta6PGDH overexpression can promote the oxidative pentose phosphate pathway (OPPP) in the cytoplasm and peroxisomes of Arabidopsis. In summary, Arabidopsis overexpressing TaG6PDH and Ta6PGDH showed improved cold tolerance.

Quantitative and qualitative determination of microplastics in oyster, seawater and sediment from the coastal areas in Zhuhai, China.

Microplastics as a new class of environmental contaminants have become the hot issue of global concern. We conducted quantitative and qualitative experiments to investigate microplastics in oyster, seawater and sediment along the Zhuhai coastline. The soft tissues of oysters were digested with potassium hydroxide (10%) and hydrogen peroxide (30%), seawaters and sediments with hydrogen peroxide (30%) to degrade organic matter, and analyzed using a digital camera, optical microscopy and micro-ATR-FTIR. The abundance of microplastics were in the range of 0.14-7.90 n/g in oysters (wet weight), 10.00-27.50 n/L in seawaters and 0.053-0.26 n/g in sediments. The fiber and fragment shape, black color, 101-500 µm of size and polyethylene composition were all classified as the major constituents of microplastics. The level of contaminants in oysters was correlated to those in their surrounding environments. Therefore, oysters may serve as a promising sentinel species for the indication of microplastic pollution in the coastal zone of Zhuhai.

[Application of near-infrared spectroscopy to agriculture and food analysis].

Near-Infrared Spectroscopy (NIRS) is the most rapidly developing and the most noticeable spectrographic technique in the 90's (the last century). Its principle and characteristics were explained in this paper, and the development of NIRS instrumentation, the methodology of spectrum pre-processing, as well as the chemical metrology were also introduced. The anthors mainly summarized the applications to agriculture and food, especially in-line analysis methods, which have been used in production procedure by fiber optics. The authors analyzed the NIRS application status in China, and made the first proposal to establish information sharing mode between central database and end-user by using network technology and concentrating valuable resources.