RESUMO
Background: Bread wheat is one of the most important food crops associated with ensuring food security and human nutritional health. The starch quality is an important index of high-quality wheat. It is affected by a complex series of factors; among which, suitable sowing time is a key factor. Aim and methods: To analyze the integrative effects of sowing time on the starch quality of high-quality wheat, in the present study, we selected a high-quality bread wheat cultivar Jinan 17 and investigated the effect of different sowing times on the starch properties and the related genes by analyzing X-ray diffraction patterns, apparent amylose content, thermal properties, pasting properties, in vitro starch digestibility, and qRT-PCR. Meanwhile, we also investigated the agronomic and yield performance that may be associated with the starch properties. Results: Delayed sowing had little effect on starch crystalline morphology, but there was a tendency to reduce the formation of crystals within wheat starch granules: (1) delayed sowing for 15 days altered the thermal properties of starch, including onset, peak and termination temperatures, and enthalpy changes; (2) delayed sowing for 30 days changed the thermal characteristics of starch relatively insignificant; (3) significant differences in pasting characteristics occurred: peak viscosity and hold-through viscosity increased, while final viscosity, breakdown viscosity, and setback viscosity tended to increase and then decrease, suggesting that delayed sowing caused changes in the surface of the starch granules resulting in a decrease in digestibility. Analysis of related genes showed that several key enzymes in starch biosynthesis were significantly affected by delayed sowing, leading to a reduction in apparent straight-chain starch content. In addition to starch properties, thousand-kernel weight also increased under delayed sowing conditions compared with normal sowing. Conclusion: The impact of delayed sowing on starch quality is multifaceted and complex, from the fine structure, and functional properties of the starch to the regulation of key gene expression. Our study holds significant practical value for optimizing wheat planting management and maximizing the potential in both quality and yield.
RESUMO
OBJECTIVE: To investigate the effect of application and clinical value of use of epinephrine in graduate increased dosage according to the equation G=(K+2n-1)mg/3 minutes (K=1,2,n=1,2...5,G< or =0.2 mg/kg) combined with aminophylline in cardiopulmonary resuscitation (CPR). METHODS: Three hundred and seventy-six patients with sudden cardiac arrest(CA) were randomly divided into 3 groups. Epinephrine and aminophylline were given through cubital vein with following methods: (1)CONTROL: (n=130). 1 mg of adrenaline was given as the first treatment. Repeat the same every 3 minutes if there was no effect. (2) In one hundred and thirty cases, the first dose of epinephrine was K=1 mg (n=122), K=2 mg (n=124). In K=1 mg group,epinephrine 1 mg and aminophylline 7 mg/kg were given as the first dose. If it was not effective, increasing dosage of epinephrine in order of 2, 3...17 mg and aminopyrine 7 mg/kg was given intravenously successively every 3 minutes (K=1, 2, n=1, 2...5). In group 2 (group equation 2), epinephrine 2 mg and aminophylline 7 mg/kg were given rapidly intravenously. If not effective, the drugs were repeated according to the equation intravenously every 3 minutes. When the dose of epinephrine exceeded 0.2 mg/kg, it should be stopped. Electrocardiogram, mean arterial pressure (MAP), the heart rate (HR), and the time of recovery of spontaneous circulation (+ROSC) were monitored, and they were evaluated for the effectiveness of resuscitation. RESULTS: (1)+ROSC rate (91.13%, 88.52%), the 24-hour survival rate (85.48%, 67.21%), the survival rate (49.19%, 31.15%), and the Glasgow coma scores [(13.12+/-1.27)scores, (12.28+/-1.32) scores] were all significantly elevated in groups in which patients received the modified regime compared with the control group [26.92%, 25.39%, 12.31% and (9.08+/-1.13) scores, all P<0.01]. The average time for +ROSC in the equation 2 and 1 groups was (8.93+/-3.27) minutes and (8.25+/-5.25) minutes, respectively, and they were significantly shorter than those of the control group [(39.25+/-9.75) minutes, both P<0.01]. (2) The average dose of the epinephrine was much reduced in achieving +ROSC in two groups with modified regimes as compared with control group [(11.75+/-3.25) mg and (13.85+/-5.15) mg, respectively vs. (24.65+/-4.35) mg, both P<0.05], and the number of using application epinephrine via intravenous from the CPR initial stage to +ROSC in the equation 2 and 1 groups was much significantly decreased compared with the control group [(3.45+/-0.55) times and (3.85+/-0.75) times vs. (18.25+/-0.75) times, both P<0.01]. CONCLUSION: The newly formed regime has better effects in increasing significantly the success rate of cardiac-resuscitation, the survival rate, and it also shortens the time for successful recovery of spontaneous circulation. It can improve the recovery of brain and nervous system function.
