RESUMO
As the largest cultivated fiber crop in the world, cotton (Gossypium hirsutum) is often exposed to various biotic stresses during its growth periods. Verticillium wilt caused by Verticillium dahliae is a severe disease in cotton, and the molecular mechanism of cotton resistance for Verticillium wilt needs to be further investigated. Here, we revealed that the cotton genome contains nine types of GST genes. An evolutionary analysis showed that a newly identified cluster (including Gh_A09G1508, Gh_A09G1509 and Gh_A09G1510) located on chromosome 09 of the A-subgenome was under positive selection pressure during the formation of an allotetraploid. Transcriptome analysis showed that this cluster participates in Verticillium wilt resistance. Because the Gh_A09G1509 gene showed the greatest differential expression in the resistant cultivar under V. dahliae stress, we overexpressed this gene in tobacco and found that its overexpression resulted in enhanced Verticillium wilt resistance. Suppression of the gene cluster via virus-induced gene silencing made cotton plants of the resistant cultivar Nongda601 significantly susceptible. These results demonstrated that the GST cluster played an important role in Verticillium wilt resistance. Further investigation showed that the encoded enzymes of the cluster were essential for the delicate equilibrium between the production and scavenging of H2 O2 during V. dahliae stress.
Assuntos
Resistência à Doença/genética , Glutationa Transferase/genética , Gossypium/genética , Família Multigênica/genética , Doenças das Plantas/microbiologia , Verticillium/patogenicidade , Arabidopsis/genética , Cacau/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Genoma de Planta/genética , Glutationa Transferase/classificação , Peróxido de Hidrogênio/metabolismo , Vitis/genéticaRESUMO
OBJECTIVE: To explore the feasibility of total phenolics from Abnormal Savda Munziq on treating human cervical carcinoma. METHODS: MTT assay was used to determine the growth inhibition ratio and IC50 on Hela cells and SiHa cells after treated with different concentrations of total phenolics. Morphologic changes of cells were observed by inverted microscope. RESULTS: Total phenolics inhibited the proliferation of Hela cells in the concentration range of 25 - 125 microg/mL; Total phenolics inhibited the proliferation of SiHa cells in the concentration range of 75 - 175 microg/mL; The inhibitory actions of total phenolics on the Hela and SiHa appeard dose-effect relationship and time-effect relationship obviously (P < 0.01); The IC50 of Hela was (125.26 +/- 16.15) microg/mL after 48h total phenolics treatment; The IC50 of SiHa was (134.51 +/- 2.55) microg/mL after 48h total phenolics treatment. And there was no statistical sense in the disparation of them (P > 0.05); Both of the cells showed apoptosis character evidently after total phenolics treatment, Along with the increasing of the concentration and the action time, morphologic changes of cells were more obviously. CONCLUSION: Total phenolics could inhibit the growth of Hela cells and SiHa cells and the inhibitory actions of total phenolics on the two cells is almostly the same. Therefore, total phenolics from abnormal savda munziq is deserved to be further studied for treating human cervical carcinoma.
