Successful resuscitation and multidisciplinary management of penetrating brain injury caused by tire explosion: A case report.

RATIONALE: Penetrating brain injury (PBI) is a rare trauma that presents as a difficult and serious surgical emergency for neurosurgeons in clinical practice. Our patient was admitted with a PBI caused by a tire explosion, which is an extremely rare cause of injury. PATIENT CONCERNS: We report a case of a 28-year-old male patient who suffered a PBI when a tire exploded while it was being inflated with a high-pressure air pump. DIAGNOSES: The patient was diagnosed with PBI presenting with multiple comminuted skull fractures, massive bone fragments with foreign bodies penetrating the underlying brain tissue of the top right frontal bone, multiple cerebral contusions, and intracranial hematoma. INTERVENTIONS: Emergency combined multidisciplinary surgery was performed for the removal of the fragmented bone pieces, hematoma, and foreign bodies; decompression of the debridement flap; reconstruction of the anterior skull base; and repair of the dura mater. OUTCOMES: The patient was successfully resuscitated and discharged 1 month later and is now recovering well. LESSONS: Patients with PBI are critically ill. Therefore, timely, targeted examinations and appropriate multidisciplinary interventions through a green channel play a key role in assessing the condition, developing protocols, and preventing complications.

A mitochondria-targeting fluorescent sensor for on-off-on response to Cu2+ and ATP in cells and zebrafish.

Cupric ion (Cu2+) and adenosine triphosphate (ATP) are functionally important in mitochondria and play essential roles in many important biological processes. In this work, a mitochondria-targeting fluorescent molecule Mito-A was used as a probe to detect Cu2+ and ATP. The results showed remarkable fluorescence quenching of Mito-A in the presence of Cu2+, and then the quenched fluorescence solution gradually recovered due to the ATP binding to Cu2+ from the structure of the molecule. Mito-A has high sensitivity to Cu2+ and ATP, with limits of detection (LOD) close to 40 nM and 0.43 µM, respectively. Cell imaging experiments showed that Mito-A has good mitochondria-targeting capabilities, and can be successfully employed for imaging Cu2+ and ATP in living cells and zebrafish.