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1.
Nat Prod Res ; : 1-5, 2024 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-38501743

RESUMO

Two new megastigmane glycosides, (6 R,7E,9R)-3-oxo-α-ionyl-9-O-α-L-rhamnopyranosyl-(1''→4')-ß-D-glucopyranoside (1) and (6 R,7E,9R)-3-oxo-α-ionyl-9-O-ß-D-glucopyranosyl-(1''→6')-ß-D-glucopyranoside (2), together with six known analogues (3-8) were isolated from the leaves of Nicotiana tabacum. The structures of all metabolites were determined by comprehensive analysis of NMR and MS spectroscopic data as well as by comparison with those of previously reported. The in vitro anti-inflammatory activity of all isolates was evaluated using a lipopolysaccharide (LPS)-induced RAW264.7 cell inflammatory model, and the compounds 1, 3, 7, and 8 exhibited inhibition of LPS-induced NO production in RAW264.7 macrophage cells with IC50 values of 42.3-61.7 µM (positive control, dexamethasone, IC50 = 21.3 ± 1.2 µM).

2.
Chin J Integr Med ; 2023 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-37999887

RESUMO

OBJECTIVE: To explore the mechanism of paeoniflorin (PF) on osteoarthritis (OA) synovial inflammation from network pharmacology to experimental pharmacology. METHODS: Targets of OA were constructed by detecting the database of network database platforms (Therapeutic Target database, DrugBank and GeneCards), and the targets of PF were constructed by PubChem and Herbal Ingredients' Targets database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these co-targeted genes were conducted via Database for Annotation, Visualization, and Integrated Discovery (DAVID) database, and protein-protein interaction (PPI) networks were conducted via the search tool for the retrieval of interacting genes (STRING) database. Cell counting kit-8 (CCK-8) assay was performed to assess the potential toxicity of PF on human OA fibroblast-like synoviocytes (FLS), quantitative real-time polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA) and Western blot were used to verify the potential mechanism of PF in synovial inflammation. RESULTS: Twenty-six co-targeted genes were identified. GO enrichment results showed that these co-targeted genes were most likely localized in the cytoplasm, and the biological processes mainly involved 'cellular response to hypoxia' 'lipopolysaccharide (LPS)-mediated signaling pathway' and 'positive regulation of gene expression'. KEGG pathway analysis indicated that these co-targeted genes may function through pathways associated with 'hypoxia-inducible factor-1 (HIF-1) signaling pathway' and 'tumor-necrosis factor (TNF) signaling pathway'. The PPI network showed that the top 3 hub genes were TP53, TNF, and CASP3. Molecular docking results showed that PF was well docking with TNF. CCK-8 showed no potential toxicity of 10, 20 and 50 µmol/L PF on human OA FLS. And PF significantly decreased the expression levels of interleukin-1 ß, interleukin-6, TNF-α matrix metalloproteinase 13 (MMP13), and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) and TNF-α in LPS-induced OA FLS. CONCLUSION: PF exhibited potent anti-inflammatory effect in OA synovial inflammation.

3.
Zhongguo Gu Shang ; 33(7): 643-8, 2020 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-32700488

RESUMO

OBJECTIVE: To observe effects of Tongluo Zhitong (, TLZT) gel preparation on p53, miR-502-5p, NF-κBp65 in synovial tissue of knee osteoarthritis (KOA), and to explore mechanism of TLZT gel preparation in treating KOA. METHODS: Thirthy-six Wistar rats aged 8 weeks and weighed 200 to 220 g (meaned 208 g) were randomly divided into normal group, model group and traditional Chinese medicine (TCM) group, 12 rats in each group. KOA model was established by modified Hulth method. After 4 weeks of modeling, TCM group treated with TLZT gel preparation for external use, 3 times daily for 2 weeks;normal group and model group were fed normally without intervention. After treatment, morphological changes of specimens in each group were observed, changes of miR-502-5p in synovial tissue were detected by qPCR, and contents of p53, NF-κBp65, IL-1ß, TNF-α, MMP-13 in synovial tissue were detected by qPCR and Western Blot respectively. RESULTS: (1)Morphological observation of specimens showed that the articular cartilage in model group was hyaline and uneven, the synovial membranes were hypertrophic and proliferative with a large number of inflammatory cells infiltrating, the joint fluid was thicker in texture;the articular cartilage in TCM group was more transparent and smooth, synovial hyperplasia was mild with a small amount of inflammatory cell infiltration, the texture of articular fluid was clear and sparse. (2) Compared with normal group, content of miR-502-5p of synovial tissue in model and TCM group were increased, mRNA and expression of p53 decreased, expression of NF-κBp65, IL-1ß, TNF-α, MMP-13 increased. (3)Compared with model group, content of miR-502-5p in synovial tissue of TCM group decreased (P<0.05), mRNA and protein expression of p53 increased (P<0.05), mRNA and protein expression of NF-κBp65, IL-1ß, TNF-α, MMP-13 decreased (P<0.05). CONCLUSION: Expression of p53, miR-502 -5p, NF -κBp65 in synovial tissue is closely related to synovial hyperplasia and inflammatory reaction, TLZT gel preparation may reduce proliferation and inflammatory reaction of KOA synovium by regulating the expression of p53, miR- 502-5p, NF-κBp65 in synovial tissues.


Assuntos
MicroRNAs , Osteoartrite do Joelho , Animais , Medicamentos de Ervas Chinesas , Ratos , Ratos Wistar , Membrana Sinovial , Proteína Supressora de Tumor p53
4.
J Healthc Eng ; 2020: 8929153, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32300473

RESUMO

Metatarsal pain is a common pathological outcome in patients with a hallux valgus (HV) deformity. However, the relationship between the degree of HV deformity and metatarsal pain has not been systematically examined. The purpose of the present study was to investigate the correlation between metatarsal pain and the degree of HV deformity. Between October 2017 and September 2018, 512 HV patients (944 feet) participated in an evaluation of their HV angle (HVA) using X-ray images. The participants were divided into four groups corresponding to their HVA (<15°, 15° to 20°, 21° to 40°, or >40°). Load rate, impulse, contact duration, and contact area were measured and recorded as dynamic gait parameters using the RsScan system. Data were evaluated using SPSS statistical software. The visual analog scale (VAS) was used to assess metatarsal pain. For the four HV deformity groups, the peak value of impulse and contact duration was concentrated on the second and third metatarsals (Meta2 and Meta3) (P < 0.05); contact area was also shown on metatarsals 1, 2, and 5 (P < 0.05). Metatarsal pain on Meta2 had the highest VAS score (VAS: 6.57), followed by Meta3 (Mean VAS: 5.72). In the HV > 40° group, the load location on Meta2 was transferred to Meta1. The percent of pain attributed to Meta2 and Meta3 was also increased in this group. These findings illustrated that metatarsal pain was primarily located on Meta2 and Meta3 in the different degrees of HV deformity. This information can provide the location to target for pain relief and help guide further rehabilitation.


Assuntos
Fenômenos Biomecânicos , Hallux Valgus/complicações , Hallux Valgus/fisiopatologia , Ossos do Metatarso/fisiopatologia , Dor/etiologia , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
5.
Zhongguo Gu Shang ; 32(8): 721-726, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31533383

RESUMO

OBJECTIVE: To explore expression of ß-catenin and NF-κB signaling pathway in synovial tissue of rats with different degrees of knee osteoarthritis (KOA). METHODS: Forty-eight SPF male rats weighed (200±20) g were randomly divided into three groups, namely model group (32 rats), sham operation group (8 rats) and control group (8 rats). KOA model rats were established by Hulth method, and 8 rats were killed at 2, 4, 8, 12 weeks respectively after modeling, in order to establish KOA model rats with moderate, early, mild and severe degree. Sham operation group was only cut off capsule of knee joint and suture to exclude interference factor, control group was untreated. Behavior, synovial hyperplasia and cartilage degeneration of rats among each group were observed. Expression of NF-κB and signaling pathway and ß-catenin in synovial tissue of rats were detected by real-time PCR. RESULTS: KOA rat model was successfully established, and synovial hyperplasia was observed in KOA model at mild and early degree, and then gradually decreased; while cartilage degeneration in the early moderate and severe KOA model was significantly expressed, and gradually aggravated with time. The results of PCR showed that expression of ß-catenin in 4-week group (8.57±0.46) and 8-week group (4.23±0.09) were higher than those in control group (P<0.05); expression of TLR-2 in 2-week group (12.04±4.02) and 4-week group (8.54±2.13) were higher than those in control group(P<0.05), and TLR-4 in 2-week group(5.04±0.93), 4-week group (3.29±0.58) and 8-week group (1.63±0.12) were higher than those in control group; expression of NF-κB was significantly higher in 2-week group (10.15±2.04), 4-week group (15.97±4.17), 8-week group (7.69±1.48) and 12-week group (6.70±1.58) than that in control group (P<0.05), and expression of IL-1ß was significantly higher in 4-week group (2.79±0.25) and 8-week group (2.46±0.32) than that of control group (P<0.05). CONCLUSIONS: On the RNA expression level, both of ß-catenin and NF-κB signaling pathways are involved in synovial inflammation in KOA model rats, and they play a regulatory role in expression of IL-1ß, degeneration of KOA.


Assuntos
Osteoartrite do Joelho , Animais , Inflamação , Masculino , NF-kappa B , Ratos , Transdução de Sinais , beta Catenina
6.
Zhongguo Gu Shang ; 32(10): 914-918, 2019 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-32512961

RESUMO

OBJECTIVE: To evaluate the therapeutic effect of Isobar TTL dynamic internal fixation system on lumbar degenerative disease and to explore the postoperative fusion rate. METHODS: The clinical data of 80 patients with lumbar degenerative disease underwent Isobar TTL dynamic internal fixation system from April 2013 to January 2016 were retrospectively analyzed. There were 39 males and 41 females, aged from 28 to 69 years old with an average of 46.4 years, 42 cases of single segment and 38 cases of double segments. Visual analogue scale (VAS), Oswestry Disability Index (ODI) and the modified Macnab criteria were used to evaluate the clinical effects. The intervertebral range of motion (ROM) and intervertebral height ratio (ISR) were measured by X-ray films. According to the standard established by SUK to evaluate the intervertebral fusion of dynamic fixed segment. RESULTS: All the patients were followed up for 17 to 45 months with an average of 31.9 months, a total of 30 cases completed imaging review. VAS and ODI scores of 80 cases respectively were 9.15±1.55, 38.65±9.60 before operation, 0.55±1.18, 1.06±2.17 at 2 weeks after operation, 0.24±0.70, 0.16±0.48 at the final follow-up. The VAS and ODI scores at 2 weeks and the final follow-up were significantly lower than those preoperative(P<0.05). At the final follow-up, according to modified Macnab criteria to evaluate the clinical effect, 65 cases obtained excellent results, 14 good, 1 fair. In the 30 cases completed imaging review, the ROM of the dynamic fixed segment was decreased from (6.87±2.18)° preoperatively to (3.52±2.80)°, (3.14±2.60)° at 2 weeks after operation and at the final follow-up(P<0.05). The ROM of the adjacent segment was increased from (4.36±1.28)° preoperatively to (4.80±1.99)°, (6.54±4.83)° at 2 weeks after operation and at the final follow-up(P<0.05). The ISR of dynamic fixed segment and adjacent segment were increased from (36.73±6.36)%, (40.74±7.29)% preoperatively to (38.37±6.35)%, (41.59±7.77)% at 2 weeks postoperatively(P<0.05), and at the final follow-up were decreased to (36.58±9.53)%, (38.25±8.08)%, there was no significant difference between preoperative and the final follow-up(P>0.05). Among them, 20 cases got possible fusion and 10 cases got non-fusion, with the possible fusion rate of 66.7%. CONCLUSIONS: Isobar TTL dynamic internal fixation system has a good clinical effect in the treatment of lumbar degenerative disease. Although the ROM of adjacent segments is increased, it can effectively prevent the degeneration of the adjacent segments in the short term. And the dynamic fixation segment tends to fusion.


Assuntos
Degeneração do Disco Intervertebral , Região Lombossacral , Fusão Vertebral , Adulto , Idoso , Feminino , Humanos , Vértebras Lombares , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
7.
Zhongguo Gu Shang ; 31(12): 1108-1113, 2018 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-30583649

RESUMO

OBJECTIVE: To compare the correlation between musculoskeletal ultrasound features, dysfunction and X-ray findings in patients with knee osteoarthritis, and to analyze the pathological mechanism of soft tissue inflammation in knee osteoarthritis. METHODS: Cross-sectional method was performed in this research (Evidence level: III). The patients with knee osteoarthritis were collected according to the screening criteria from September 2016 to January 2017 in Orthopedic clinic in our hospital. Musculoskeletal ultrasound and X-ray images were obtained and measured, knee function was measured by Lysholm scale. Pearson coefficient, t test and Wilcoxon were applied to analyze the correlation between soft tissue inflammation, knee dysfunction and X-ray features. RESULTS: Total 123 patients with knee osteoarthritis were recruited in this research. Soft tissue inflammation around knee had a high incidence in patients with knee osteoarthritis (infrapatellar fat pad inflammation 81%), and the synovial membrane thickness, joint effusion depth and meniscus bulging were beyond the normal range. Correlation analysis showed that the about Lysholm score and joint effusion depth had negative correlations with "Squat" score(r=-0.21, P=0.02). and Medial meniscus bulging had negative correlations with "Sustain" score(r=-0.26, P<0.01) and Lysholm total score (r=-0.19, P=0.04). Lateral meniscus bulging had a negative correlation with "Unstable" score (r=-0.22, P=0.02). The X-ray features, and medial joint space narrow had negative correlations with joint effusion depth(r=-0.27, P<0.01) and synovial membrane thickness(r=-0.17, P=0.007), and had a positive correlation with medial meniscus bulging. Medial joint space narrow was significantly correlated with patellar ligament inflammation and fat pad inflammation(P<0.05). Lateral joint space narrow was significantly correlated with patellar ligament inflammation(P=0.02). CONCLUSIONS: Soft tissue inflammation around the knee-a major pathological manifestation of knee osteoarthritis, has significant correlations with knee dysfunction and bony structure lesions, and affects the progression of knee osteoarthritis by damaging knee joint function and promoting the destruction of articular cartilage.


Assuntos
Cartilagem Articular , Osteoartrite do Joelho , Estudos Transversais , Humanos , Articulação do Joelho , Meniscos Tibiais
8.
Zhongguo Gu Shang ; 31(10): 916-921, 2018 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-30373344

RESUMO

OBJECTIVE: To investigate the relationship between living habit and cervical instability in adolescent patients with neck pain. METHODS: Fifty-nine adolescent patients with neck pain(neck pain group) and seventeen healthy teenagers (control group) were recruited and divided into two groups, and clinical information, living habit were collected. In addition, all people were taken lateral, hyperextension and hyperflexion radiography to analyze relationship between living habit and cervical instability. RESULTS: There was no obvious difference in age, height, weight and body mass index between two groups. The neck pain group using cellphone time per day is longer than control group, while control group had more exercise time than neck pain group(P<0.01). The incidence of instability in neck pain group was significantly higher than that in control group(P<0.01). In hyperflexion, angular displacement(AD) of neck pain group in vertebral body between C3-C4, C4-C5 and C5-C6 was significantly higher than that of control group. In neck pain group, AD of hyperflexion was higher than that of hyperextension on C4-C5(P<0.01), and AD of hyperextension is higher than that of hyperflexion on C6-C7(P<0.05). In neck pain group, AD of hyperextension on C4-C5 was positively correlated with time of using cellphone every day(r=0.275, P=0.035). And AD of hyperflexion was significantly positive correlated with time of using cellphone(r=0.577, P<0.001), but was negatively correlated with exercise time(r=-0.279, P=0.032). The AD of hyperflexion on C5-C6 was negatively correlated with exercise time every day(r=-0.292, P=0.025), AD of hyperextension was negatively correlated with time of using computer every day(r=-0.262, P=0.045). CONCLUSIONS: Adolescent neck pain patients had more time to use cellphone than normal teens every day, and exercise time is less than healthy teenagers, and occurrence rate of cervical instability is higher on C3-C4, C4-C5, C5-C6 segment. The longer daily exercise time, the smaller C4-C5 and C5-C6 AD values; the longer cellphone usage every day, the greater C4-C5 AD values.


Assuntos
Instabilidade Articular , Cervicalgia , Adolescente , Vértebras Cervicais , Hábitos , Humanos , Radiografia
9.
Zhongguo Gu Shang ; 31(10): 933-936, 2018 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-30373347

RESUMO

OBJECTIVE: To investigate expression features and correlation of genes expression on MyD88-dependent signaling pathway in synovial membrane (SM) of progression of knee osteoarthritis (OA). METHODS: Sixty Wistar rats were randomly divided into 6 groups, including blank group (N), false surgical group, model groups[2 weeks (2W), 4 weeks (4W), 8 weeks (8W) and 12 weeks (12W)], with 10 rats in each group. The models were established by using Hulth method. Control group was experienced no surgery, while false surgical group was only opened joint cavity and sutured. The SM samples was collected according to the time designed above. The relative expression quantity of MyD88, TLR4 and NF-κB was detected by Real-time PCR after the extraction of the total RNA and reverse transcription. The correlation analysis was obtained by SPSS. RESULTS: There was no significant difference in each gene mRNA expression between false surgical and blank group(P> 0.05), while enhanced expression was found in the model groups(P<0.05). The correlation index among MyD88, TLR4 and NF-κB was 0.91 and 0.86 respectively, and had significant difference among them. CONCLUSIONS: Positively relative among MyD88, TLR4 and NF-κB played main role in TLR4/NF-κB signal passway, and could predicate the expression of other genes in the passway. It also could further provide the basis for clarify the pathologic mechanism of knee OA.


Assuntos
Osteoartrite do Joelho , Animais , Fator 88 de Diferenciação Mieloide , NF-kappa B , Ratos , Ratos Wistar , Transdução de Sinais , Receptor 4 Toll-Like
10.
Zhongguo Gu Shang ; 30(4): 349-352, 2017 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-29349986

RESUMO

OBJECTIVE: To observe the curative effect of musculoskeletal ultrasound-guided needle-knife on the degenerative meniscus disease, and to provide a new method in the treatment of degenerative meniscus disease. METHODS: Seventy-seven patients with degenerative meniscus disease treated in the Third Affiliated Hospital of Beijing University of Chinese Medicine from January 2015 to September 2015 were selected, including 30 males and 47 females, aged from 44 to 66 years old, with an average of 57.5 years old. VAS scores, Lysholm scores and distance of meniscal protrusion were analyzed and compared before treatment, 2 weeks and 1 month after treatment. The curative effect was summarized at last. RESULTS: The mean Lysholm scores were 51.63±15.26(before treatment), 77.13±11.82(2 weeks after treatment) and 87.56±8.65(1 month after treatment). The mean VAS scores were 7.080±1.574 (before treatment), 2.630±0.310(2 weeks after treatment) and 0.850±0.177(1 month after treatment). The mean of the distance of meniscal protrusion scores were 0.400±0.156 (before treatment), 0.298±0.140 (2 weeks after treatment) and 0.240±0.110 (1 month after treatment). VAS scores and Lysholm scores were improved significantly compared with preoperative results. The distance of meniscal protrusion showed an obvious improvement after treatment. CONCLUSIONS: The treatment of musculoskeletal ultrasound-guided needle-knife has advantages of high accuracy position and excellent effectiveness for degenerative meniscus disease. The treatment provides safety operation and significantly improves quality of life in patients without any complications.


Assuntos
Doenças das Cartilagens/cirurgia , Meniscos Tibiais , Instrumentos Cirúrgicos , Adulto , Idoso , Artroscopia , Feminino , Humanos , Articulação do Joelho , Masculino , Pessoa de Meia-Idade , Qualidade de Vida , Resultado do Tratamento , Ultrassonografia de Intervenção
11.
Zhongguo Gu Shang ; 29(5): 429-33, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-27505959

RESUMO

OBJECTIVE: To analyze and compare the characteristics of musculoskeletal ultrasonography and X-ray of knee osteoarthritis, and to investigate the advantages of them. METHODS: According to the inclusion and exclusion criteria, 57 cases (66 knees) were collected from February 2015 to May 2015. Among them, there were 48 females and 9 males with an average age of (58.9 +/- 9.8) years old (ranged, 41 to 78 years old). The main symptoms included unilateral or bilateral knee pain and locked joints explicit areas of tender points. The mean course of disease was (13.6 +/- 3.0) months. The results of musculoskeletal ultrasound and X-ray examinations were analyzed. RESULTS: According to Kellgren-Lawrence classification of knee joint on the X-ray: the musculoskeletal ultrasound results of patients with I degree synovial hyperplasia in 9 cases, joint effusion in 20 cases, meniscal disease in 13 cases, patellar pad inflammation in 5 cases, and patellar lesion in 8 cases. The musculoskeletal ultrasound results of patients with III degree: synovial hyperplasia in 20 cases,joint effusion in 31 cases, meniscal disease in 22 cases, patellar pad inflammation in 16 cases and patellar lesion in 17 cases. The musculoskeletal ultrasound results of patients with III degree: synovial hyperplasia in 6 cases,joint effusion in 6 cases, meniscal disease in 7 cases, patellar pad inflammation in 7 cases and patellar lesion in 5 cases. CONCLUSION: The musculoskeletal ultrasound can detect the pathological changes of knee soft tissue sensitively, provide an accurate location of lesions,and find lesions early. The musculoskeletal ultrasound should be applicated in the diagnosis of knee osteoarthritis.


Assuntos
Osteoartrite do Joelho/diagnóstico por imagem , Adulto , Idoso , Feminino , Humanos , Articulação do Joelho/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Músculo Esquelético/diagnóstico por imagem , Osteoartrite do Joelho/diagnóstico , Radiografia , Ultrassonografia
12.
Int J Med Mushrooms ; 18(3): 253-60, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27481159

RESUMO

White jelly mushroom, Tremella fuciformis, is a popular edible mushroom with interesting medicinal properties (e.g., immunostimulating, antidiabetic). The formation of T. fuciformis basidiomes is highly dependent on the presence of a specific host fungus, both in nature and for industrial production. This host has traditionally been indicated as "Xianghui" in China, yet which or how many fungal species Xianghui comprises is unclear, with various authorities claiming different species. At present, Annulohypoxilon archeri is generally assumed to be the main Xianghui species, but this has not yet been confirmed experimentally. The implementation of older, premolecular-based research data (i.e., morphological) with present, sequence-based data to solve the identity remains confusing and studies addressing both identification methods in combination are lacking. The unclear identity of Xianghui is a major obstacle for further understanding of the important relationship between the host(s) and T. fuciformis. In this study, we collected a wild specimen of T. fuciformis together with several nearby stroma of Xianghui, cocultivated T. fuciformis with the Xianghui isolates, and observed basidiome formation. Internal transcribed spacer (ITS) sequence analysis showed that all Xianghui spore isolates belonged to the same species and both morphological analysis of sexual stages and ITS ß-tubulin and actin gene sequences of the Xianghui specified it as Annulohypoxylon stygium. The ITS sequences of the newly identified Xianghui further closely matched those of the Xianghui strains used in the mushroom industry, showing that wild and culture spawn Xianghui in China consist of A. stygium. In contrast with previous conclusions, A. stygium, and not A. archeris, seems to be the preferred host of T. fuciformis.


Assuntos
Basidiomycota/classificação , Xylariales/classificação , Basidiomycota/citologia , Basidiomycota/genética , Carpóforos , Filogenia , Análise de Sequência de DNA , Xylariales/citologia , Xylariales/genética
14.
Zhongguo Gu Shang ; 28(7): 628-32, 2015 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-26399105

RESUMO

OBJECTIVE: To preliminarily research the formular about the Traditional Chinese Medicine (TCM) syndrome of adolescent neck pain. METHODS: An observation table of adolescent neck pain syndromes was formulated,and 1 397 patients with adolescent neck pain were investigated to establish a database of adolescent neck pain. The Descriptive Statistical Analysis and Hierarchical Cluster Analysis were performed by statistical software. RESULTS: Totally 60 TCM symptoms was clustered into 4 TCM syndromes by Hierarchical Cluster Analysis. The expert panel of TCM syndromes preliminarily formulate 4 TCM syndromes of adolescent neck pain by analyzing the result of Cluster Analysis and discussing their clinical experience. CONCLUSION: Adolescent neck pain is a category of Tendon Trauma's Bi-syndrome of TCM. Ying, Wei, Qi and blood block caused by exopathy, strains, and internal injury is considered as the main pathogenesis of adolescent neck pain. Base on statistical result and expert's opinions, 4 TCM syndromes about adolescent neck pain were formulated: cold-dampness syndrome, dampness-heat blockage syndrome, liver-stagnation and spleen-deficiency syndrome, Qi and Yin deficiency of both heart and kidney syndrome.


Assuntos
Cervicalgia/diagnóstico , Adolescente , Adulto , China/epidemiologia , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Medicina Tradicional Chinesa , Cervicalgia/epidemiologia , Adulto Jovem
15.
Zhongguo Gu Shang ; 28(1): 90-5, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-25823143

RESUMO

Osteoarthritis (Osteoarthritis, OA) is a common clinical degenerative joint disease with increased incidence rate in recent years. Animal experiment is one of the important ways to explore pathogenesis and treatment of OA, while induced animal model is the most important part in animal experiment. Intra-articular injection of drugs is a classical method for establishing animal model of OA. Choose of animal should follows the principle of correlation, appropriateness and practicability, injections should perform in accordance with experimental purposes and subject, detections means and evaluation methods also should corresponding to experimental reality. The gold standard of OA animal model and intra-articular injections has not build, need further study.


Assuntos
Modelos Animais de Doenças , Osteoartrite/etiologia , Animais , Citocinas/análise , Injeções Intra-Articulares , Camundongos , Osteoartrite/diagnóstico , Osteoartrite/imunologia , Coelhos , Ratos
16.
Zhongguo Gu Shang ; 27(1): 17-20, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24754138

RESUMO

OBJECTIVE: To study soft tissue changes observed through musculoskeletal ultrasound (MSUS) in the treatment of knee osteoarthritis with needle-knife, so as to provide MSUS basis for needle-knife in the treatment of knee osteoarthritis. METHODS: Forty patients with knee osteoarthritis treated in the Third Affiliated Hospital of Beijing University of Chinese Medicine from December 2011 to December 2012 were selected according to inclusion and exclusion criteria. All the patients were treated with needle-knife release method. The VAS scores and knee joint circumference were recorded before treatment and 2 weeks after treatment. The changes of knee joint hydrops articuli and joint synovial thickness were measured through MSUS. RESULTS: The knee pain index was 6.850 +/- 1.417 before treatment and 2.790 +/- 1.299 after treatment;the index after treatment was lower than that of before treatment. The knee joint circumference was 407.320 +/- 45.151 mm before treatment and 391.240 +/- 41.129 mm after treatment; the knee joint circumference decreased after treatment. The amount of hydrops articuli observed by musculoskeletal ultrasound showed that 47 knees were cured, 19 knees improved and 2 knees failed. The synovial membrane thickness: 43 knees cured, 17 knees improved and 8 knees failed. CONCLUSION: The hydrops articuli and synovial thickness of knee joint of patients with knee osteoarthritis observed under the MSUS is consistent with the main symptoms and signs, which suggests that MSUS observation on soft tissue changes before and after needle knife in the treatment of knee osteoarthritis with high reliability.


Assuntos
Articulação do Joelho/diagnóstico por imagem , Articulação do Joelho/patologia , Agulhas , Osteoartrite do Joelho/patologia , Osteoartrite do Joelho/cirurgia , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite do Joelho/complicações , Osteoartrite do Joelho/diagnóstico por imagem , Dor/complicações , Membrana Sinovial/patologia , Resultado do Tratamento , Ultrassonografia
17.
Clinics (Sao Paulo) ; 69(4): 234-40, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24714830

RESUMO

OBJECTIVE: The aim of this article was to determine the effects of minimally invasive percutaneous plates versus interlocking intramedullary nailing in the treatment of tibial shaft fractures in adults. METHOD: Literature searches of the Cochrane Library, PubMed, EMBASE, the Chinese Biomedical Literature database, the CNKI database, Wanfang Data, and the Weipu Journal database were performed up to August 2013. Only randomized and quasi-randomized controlled clinical trials comparing the use of percutaneous plates and interlocking intramedullary nails for tibial shaft fractures were included. Data collection and extraction, quality assessment, and data analyses were performed according to the Cochrane standards. RESULTS: Eleven trials were included. Compared with interlocking intramedullary nailing, minimally invasive percutaneous plates shortened fracture healing time and resulted in lower rates of postoperative delayed union and pain. There was no significant difference between the two methods with regard to the rates of excellent and good Johner-Wruh scoring, the rate of reoperation, and other complications. CONCLUSIONS: Overall, insufficient evidence exists regarding the effects of minimally invasive percutaneous plates versus interlocking intramedullary nailing in the treatment of tibial shaft fractures in adults. Low-quality evidence suggests that minimally invasive percutaneous plates could shorten fracture healing time, decrease the rate of postoperative delayed union, and decrease pain levels compared with interlocking intramedullary nailing. There is no significant difference between the two groups in terms of functional recovery scores, reoperation, and other complications. Further research that includes high-quality randomized controlled, multicenter trials is required to compare the effects of minimally invasive percutaneous plates versus interlocking intramedullary nailing in the treatment of tibial shaft fractures in adults.


Assuntos
Pinos Ortopédicos , Placas Ósseas , Fixação Intramedular de Fraturas/métodos , Fraturas da Tíbia/cirurgia , Adulto , Feminino , Fixação Intramedular de Fraturas/instrumentação , Consolidação da Fratura , Humanos , Masculino , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Recuperação de Função Fisiológica , Medição de Risco , Fatores de Tempo , Resultado do Tratamento
18.
Clinics ; 69(4): 234-240, 4/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-705778

RESUMO

OBJECTIVE: The aim of this article was to determine the effects of minimally invasive percutaneous plates versus interlocking intramedullary nailing in the treatment of tibial shaft fractures in adults. METHOD: Literature searches of the Cochrane Library, PubMed, EMBASE, the Chinese Biomedical Literature database, the CNKI database, Wanfang Data, and the Weipu Journal database were performed up to August 2013. Only randomized and quasi-randomized controlled clinical trials comparing the use of percutaneous plates and interlocking intramedullary nails for tibial shaft fractures were included. Data collection and extraction, quality assessment, and data analyses were performed according to the Cochrane standards. RESULTS: Eleven trials were included. Compared with interlocking intramedullary nailing, minimally invasive percutaneous plates shortened fracture healing time and resulted in lower rates of postoperative delayed union and pain. There was no significant difference between the two methods with regard to the rates of excellent and good Johner-Wruh scoring, the rate of reoperation, and other complications. CONCLUSIONS: Overall, insufficient evidence exists regarding the effects of minimally invasive percutaneous plates versus interlocking intramedullary nailing in the treatment of tibial shaft fractures in adults. Low-quality evidence suggests that minimally invasive percutaneous plates could shorten fracture healing time, decrease the rate of postoperative delayed union, and decrease pain levels compared with interlocking intramedullary nailing. There is no significant difference between the two groups in terms of functional recovery scores, reoperation, and other complications. Further research that includes high-quality randomized controlled, multicenter trials is required to compare the effects of minimally invasive percutaneous plates versus interlocking intramedullary nailing in the treatment of tibial shaft fractures in adults. .


Assuntos
Adulto , Feminino , Humanos , Masculino , Pinos Ortopédicos , Placas Ósseas , Fixação Intramedular de Fraturas/métodos , Fraturas da Tíbia/cirurgia , Consolidação da Fratura , Fixação Intramedular de Fraturas/instrumentação , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Recuperação de Função Fisiológica , Medição de Risco , Fatores de Tempo , Resultado do Tratamento
19.
Zhongguo Gu Shang ; 26(1): 19-23, 2013 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-23617136

RESUMO

OBJECTIVE: To observe X-ray features of before and after treatment of cervical imbalance syndrome with osteopathy and traction intervention in youth patients, then to investigate the clinical effect of the treatment of the cervical imbalances syndrome in youth by osteopathy. METHODS: From September 2007 to December 2010, one hundred and eighty-seven students of 19 to 22 years (means 21 years) with neck pain as the main symptom in Beijing university of Chinese medicine were selected and divide them into osteopathy group and traction groups randomly. In osteopathy group, there were 94 patients including 40 males and 54 females; in traction group, there were 93 patients including 42 males and 51 females. The treatment of osteopathy group lasted for 3 weeks,three times per week; traction group by traction treatment three weeks,three times a week, too. X-ray before treatment and 3 weeks after treatment were collected. RESULTS: The osteopathy group:94 cases, before treatment, abnormal curvature in 57 cases, spinous position change in 45 cases and angular displacement in 44 cases, vertebral sliding in 15 cases, Ruth Jackson line intersect proneness change in 70 cases,stretch change in 47 cases; after treatment, abnormal curvature in 35 cases, spinous position change in 24 cases and angular displacement in 18 cases, vertebral sliding in 3 cases, Ruth Jackson line intersect proneness change in 41 cases, extension change in 33 cases; X-Ray measurement results: cervical curvature improved from (7.070 +/- 4.629) degrees before treatment to (7.660 +/- 4.156) degrees after treatment, angular displacement improved from (13.790 +/- 2.590) degrees before treatment to (11.050 +/- 2.560) degrees after treatment; vertebral sliding improved from (3.770 +/- 0.350) mm before treatment to (3.160 +/- 0.485) mm after treatment. The change of angular displacement and vertebral sliding were so significant before and after treatment in this group, there were statistical defferences between before and after the treatment (P < 0.01) in osteopathy group. The traction group: 93 cases, before treatment,abnormal curvature in 60 cases, spinous position change in 39 cases and angular displacement in 39 cases, vertebral sliding in 15 cases, Ruth Jackson line intersect proneness change in 70 cases, stretch in 47 cases; after treatment,abnormal curvature in 50 cases, spinous position change in 29 cases and angular displacement in 17 cases; vertebral sliding in 3 cases, Ruth Jackson line intersect proneness change in 41 cases, stretch in 33 cases; X-Ray measurement results: cervical curvature improved from (5.590 +/- 4.639) degrees before treatment to (5.990 +/- 4.330) degrees after treatment, angular displacement improved from (13.360 +/- 2.064) degrees before treatment to (11.210 +/- 1.872) degrees after treatment; vertebral sliding improved from (3.790 +/- 0.339) mm before treatment to (3.480 +/- 0.332) mm after treatment. The change of angular displacement and vertebral sliding were also so significant in traction group before and after treatment, there were statistical defferences between before and after the treatment (P < 0.01). CONCLUSION: (1) Pathological changes can be called youth cervical imbalance syndrome. (2) Stress points, angular displacement, cervical vertebral slip, curvature straightened, spinous position change are main X-ray performances. (3) Both osteopathy and traction intervention on are efficient in youth neck pain. (4) Youths cervical vertebra imbalance is early but reversible performance.


Assuntos
Vértebras Cervicais/diagnóstico por imagem , Instabilidade Articular/diagnóstico por imagem , Doenças da Coluna Vertebral/diagnóstico por imagem , Tração , Feminino , Humanos , Instabilidade Articular/terapia , Masculino , Radiografia , Doenças da Coluna Vertebral/terapia , Raios X , Adulto Jovem
20.
Zhongguo Gu Shang ; 25(5): 373-6, 2012 May.
Artigo em Chinês | MEDLINE | ID: mdl-22870679

RESUMO

OBJECTIVE: To perform Multiple Linear Regression analysis of X-ray measurement and WOMAC scores of knee osteoarthritis, and to analyze their relationship with clinical and biomechanical concepts. METHODS: From March 2011 to July 2011, 140 patients (250 knees) were reviewed, including 132 knees in the left and 118 knees in the right; ranging in age from 40 to 71 years, with an average of 54.68 years. The MB-RULER measurement software was applied to measure femoral angle, tibial angle, femorotibial angle, joint gap angle from antero-posterir and lateral position of X-rays. The WOMAC scores were also collected. Then multiple regression equations was applied for the linear regression analysis of correlation between the X-ray measurement and WOMAC scores. RESULTS: There was statistical significance in the regression equation of AP X-rays value and WOMAC scores (P<0.05), while there was no statistical significance in the regression equation of lateral X-ray value and WOMAC scores (P>0.05). CONCLUSION: 1) X-ray measurement of knee joint can reflect the WOMAC scores to a certain extent. 2) It is necessary to measure the X-ray mechanical axis of knee, which is important for diagnosis and treatment of osteoarthritis. 3) The correlation between tibial angle,joint gap angle on antero-posterior X-ray and WOMAC scores is significant, which can be used to assess the functional recovery of patients before and after treatment.


Assuntos
Osteoartrite do Joelho/diagnóstico por imagem , Adulto , Idoso , Humanos , Modelos Lineares , Pessoa de Meia-Idade , Radiografia , Raios X
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