Modification of polyacrylonitrile-derived carbon nanofibers and bacteriophages on screen-printed electrodes: A portable electrochemical biosensor for rapid detection of Escherichia coli.

A facile method was developed for fabricating a disposable phage-based electrochemical biosensor for the detection of Escherichia coli. Bare screen-printed electrodes (SPEs) were modified using a two-step drop-casting method, in which polyacrylonitrile-derived electrospun carbon nanofibers (CNFs) were deposited, followed by E. coli bacteriophage immobilization. The deposition of CNFs increased the surface area for bacteriophage immobilization while maintaining a conductive link for ferro/ferricyanide redox transitions. Cyclic voltammetry and electrochemical impedance spectroscopy confirmed that the CNF modification increased the electron-transfer rate, whereas bacteriophages and E. coli blocked electron transfer at the electrode. The biosensor achieved a response within 10 min and a linear response in the E. coli concentration range of 102-106 CFU/mL. A limit of detection (LOD) of 36 CFU/mL in phosphate-buffered saline was achieved, which is the lowest LOD reported thus far for phage-based disposable SPE sensors. The biosensor exhibited recovery rates between 106 % and 119 % for E. coli detection in apple juice. The proposed fabrication method allowed electrodes to be obtained from different production batches with remarkable consistency and reproducibility, and they remained stable at room temperature for one month. Thus, a phage-based disposable SPE that can be used for bacterial detection was developed for the first time.

Clinical features and surgical outcomes in young children with focal cortical dysplasia type II.

AIMS: To investigate clinical characteristics and surgery outcomes of young children with focal cortical dysplasia (FCD) type II. METHODS: Young children (onset age ≤6 years) with FCDII who underwent epileptic surgery in Children Epilepsy Center of Peking University First Hospital in 2014-2018 were followed up for at least 6 months after surgery. RESULTS: One hundred and twelve children with FCDII were included, with median age of onset 0.9 years (0.01-5.9), who underwent surgery at 4.1 years old (0.8-16.2). Focal seizures were most frequent (90.2%) and epileptic spasms presented in 23 (20.5%) cases. Epileptic encephalopathy was not uncommon (12.5%), associated with earlier epilepsy onset and higher rate of bilateral onset on ictal EEG (OR = 0.213, 9.059; P = .041, .004). At the last follow-up, 88.4% achieved seizure-free. Before surgery, 49.1% showed moderate/severe developmental delay, associated with earlier seizure onset and higher rate of history of epileptic encephalopathy (OR = 0.740, 5.160, P = .023, .042). For 48 children with preoperatively moderate/severe developmental delay, DQ rank at 6 months postsurgery was improved in only four cases. CONCLUSION: For young children with FCDII, they tend to present with epileptic encephalopathies and show moderate/severe developmental delay before surgery. The seizure outcome was favorable after surgery. For children with preoperatively moderate/severe developmental delay, developmental outcome at 6 months after surgery was not satisfactory.

[Surgery for pediatric intractable epilepsy due to posterior quadrantic cortical dysplasia].

OBJECTIVE: To investigate the clinical features and surgical strategy for pediatric intractable epilepsy due to posterior quadrantic cortical dysplasia and to assess the surgical outcomes. METHODS: The clinical features and preoperative evaluation results of 14 children with intractable epilepsy due to posterior quadrantic cortical dysplasia were retrospectively analyzed. The localization values of video-electroencephalography and intraoperative monitoring and the indications, advantages and disadvantages of temporoparietooccipital disconnection were evaluated. RESULTS: The 14 children had different seizure types, of which spasm was the most common one. The lesions of cortical dysplasia involved the central cerebral region in 2 cases. After temporoparietooccipital disconnection in 14 patients, 13 cases were seizure-free; only one case still had seizures, but the frequency dropped by more than 50%. CONCLUSIONS: Temporoparietooccipital disconnection is a safe and effective surgical procedure for children with intractable epilepsy due to posterior quadrantic cortical dysplasia.

The bacterial diversity in an anaerobic ammonium-oxidizing (anammox) reactor community.

An anaerobic ammonium-oxidation (anammox) reactor was operated for more than 500 days and the anammox activity of the biomass in the reactor reached 0.58 kg N(total)/kg VSS d. The removal ratios of NO2(-)-N to NH4(+)-N in both reactor and activity tests were nearly 1.1. The bacterial diversity in the reactor was investigated by analysis of 16S rRNA gene clone libraries and quantitative real-time PCR (qPCR). The analysis showed that more than half of the clones in the library were affiliated to recognized filamentous bacteria. The previously recognized anammox bacterium (AnAOB) Candidatus Kuenenia stuttgartiensis was only detected by using a Planctomycetes-specific 16S rRNA gene primer set. However, at least two different types of AnAOB were detected by the primer set targeting the hydrazine-oxidizing enzyme gene (hzo). The aerobic ammonium-oxidizing bacteria (AAOB) Nitrosomonas europaea-eutropha group, which is widely detected in oxygen-limited environments, was also found in this reactor. The result of qPCR indicated that AnAOB comprised 16% of the community population while AAOB comprised less than 1% in the reactor.