RESUMO
The United States and Norway have approximately the same per capita Gross Domestic Product (GDP) and average personal income, but their per capita health spending patterns are quite different. In 1982, the US spent 6.5% of its total health expenditures on dental services while Norway spent 5.4%. A higher percentage of Norwegian adults see a dentist annually as compared to US adults. In 1984, the mean net income of dentists in private practice was $66,940 in the US and $27,125 in Norway; this is respectively 5 and 1 3/4 times the average per capita income in those countries. The American publicly-employed dentist earned approximately two-thirds of what the American private practitioner made, while still earning approximately 50% more than his Norwegian counterpart. Some basic information concerning the ratios of dentists, specialists, and dental hygienists to the population is given. The relative proportion of women dentists in the two countries is contrasted. Finally, data on graduates from the dental schools, enrollment cuts, and estimated dentist to population ratios by the year 2000 are described to compare future manpower that will be available to the two countries. Several dissimilarities in the political and social systems are described and discussed. It is emphasized that caution should be used when interpreting and comparing data about countries with different dental delivery, political, and social systems.
Assuntos
Serviços de Saúde Bucal/economia , Odontólogos/economia , Gastos em Saúde , Renda , Honorários Odontológicos/estatística & dados numéricos , Feminino , Gastos em Saúde/estatística & dados numéricos , Humanos , Renda/estatística & dados numéricos , Masculino , Noruega , Administração da Prática Odontológica/economia , Estados UnidosRESUMO
A survey was conducted among U.S. dental schools to determine the status of geriatric dentistry in the curriculum. Since a comparable, independent study was done in 1979, there has been significant growth in formalized didactic courses and less reliance on incorporating geriatric content as lectures or other components of existing courses. The growth in specialized courses was accompanied by an increased tendency to supplement the clinical topics with others relating to social and behavioral aspects of treating geriatric patients.
Assuntos
Currículo , Educação em Odontologia , Odontologia Geriátrica/educação , Docentes de Odontologia , Humanos , Porto Rico , Faculdades de Odontologia/organização & administração , Ensino/métodos , Estados UnidosAssuntos
Educação em Odontologia , Odontologia Geriátrica/educação , Idoso , Envelhecimento , Humanos , Saúde Bucal , Estados UnidosAssuntos
Projetos de Pesquisa , Coleta de Dados , População , Pesquisa , Apoio à Pesquisa como AssuntoRESUMO
Stimulated histamine release was depressed at least tenfold in mitotic 2H3 rat basophilic cells when compared with interphase cells even though both contained comparable amounts of histamine. Antigen stimulation of IgE-sensitized interphase cells initiated an influx of Ca2+ that preceded secretion of histamine and a similar Ca2+ influx occurred in stimulated mitotic cells. This strongly suggests that during mitosis there is a dramatic inhibition of one or more of the steps on the pathway leading from elevated intracellular Ca2+ to the fusion of secretory granules with the plasma membrane.
Assuntos
Liberação de Histamina , Mastócitos/imunologia , Mitose , Animais , Benzimidazóis/farmacologia , Cálcio/metabolismo , Linhagem Celular , Liberação de Histamina/efeitos dos fármacos , Interfase/efeitos dos fármacos , Cinética , Mastócitos/fisiologia , Mitose/efeitos dos fármacos , Nocodazol , Ovalbumina/farmacologia , RatosRESUMO
The Health Professions Capitation Grant program was in existence for nine years. At its inception the capitation grant program was intended principally to be an incentive program for various health professions schools, dental schools among them, to increase the output of manpower. In recent years, the program had changed its emphasis for dental schools to curricular modifications designed to improve the geographic and specialty distribution of dental graduates. All the same time the appropriations for the program had steadily declined since 1972. These developments and the legislative actions that led to the termination of the capitation program are reviewed.
Assuntos
Faculdades de Odontologia/economia , Apoio ao Desenvolvimento de Recursos Humanos/história , Governo , História do Século XX , Faculdades de Odontologia/normas , Apoio ao Desenvolvimento de Recursos Humanos/legislação & jurisprudência , Estados UnidosRESUMO
A population of sarcoplasmic-reticulum vesicles, all of which were sealed with their cytoplasmic side outwards, was obtained by density-gradient centrifugation after loading the vesicles with calcium oxalate. The calcium oxalate could subsequently be removed from the vesicles by the reverse action of the calcium-transport system. Measurements of the catalysed exchange of the phosphatidylcholine in the sarcoplasmic-reticulum cytoplasmic monolayer with an exogenous phosphatidylcholine pool suggested that phosphatidylcholine is symmetrically distributed across the sarcoplasmic-reticulum membrane. A similar result was obtained for phosphatidylethanolamine when sarcoplasmic-reticulum lipids were labelled with trinitrobenzenesulphonic acid. Further catalysed lipid-exchange reactions showed that the transverse movement of phosphatidylcholine across the membrane was exceedingly slow (t 1/2 greater than 15 days).
Assuntos
Bicamadas Lipídicas/metabolismo , Retículo Sarcoplasmático/metabolismo , Animais , Cálcio/metabolismo , Citoplasma/metabolismo , Cinética , Microscopia Eletrônica , Fosfatidilcolinas/metabolismo , Fosfatidiletanolaminas/metabolismo , Coelhos , Retículo Sarcoplasmático/ultraestrutura , Ácido Trinitrobenzenossulfônico/metabolismoRESUMO
The interaction of the n-alkanols with lipid bilayers and excitable membranes shows that there is no simple correlation between conduction block and any of the perturbations of bilayer structure currently proposed as unitary mechanisms of local anaesthetic action. We propose instead that the n-alkanols act by direct interaction with target proteins to cause perturbations which depend directly on the precise structure of the alcohol.
Assuntos
Álcoois/farmacologia , Anestésicos Locais/farmacologia , Fluidez de Membrana/efeitos dos fármacos , Proteínas de Membrana , Condução Nervosa/efeitos dos fármacos , Animais , Fenômenos Químicos , Físico-Química , Cobaias , Técnicas In Vitro , Membranas Artificiais , Modelos Biológicos , Proteínas do Tecido Nervoso , Conformação Proteica/efeitos dos fármacos , Relação Estrutura-Atividade , TemperaturaRESUMO
We have replaced the lipid associated with a purified calcium transport protein with a series of defined synthetic dioleoyl phospholipids in order to determine the effect of phospholipid headgroup structure on the ATPase activity of the protein. At 37 degrees C the zwitterionic phospholipids (dioleoyl phosphatidylcholine and dioleoyl phosphatidylethanolamine) support the highest activity, while a phospholipid with two negative charges (dioleoyl phosphatidic acid) supports an activity which is at least twenty times lower. Dioleoyl phospholipids with a single net negative charge support at intermediate ATPase activity which is not affected by the precise chemical structure of the phospholipid headgroup. The protocol used to determine the phospholipid headgroup specificity of calcium transport protein is novel because it establishes the composition of the lipid in contact with the protein without the need to isolate defined lipid-protein complexes. This allows the lipid specificity to be determined using only very small quantities of test lipids. We also determined the ability of the same phospholipids to support calcium accumulation in reconstituted membranes. Two requirements had to be met. The phospholipid had to support the ATPase activity of the pump protein and it had to form sealed vesicles as determined by electron microscopy. Since a number of phospholipids met those requirements it is clear that in vitro the lipid specificity of the calcium-accumulating system is rather broad.
Assuntos
Adenosina Trifosfatases/metabolismo , Cálcio/metabolismo , Proteínas de Transporte/metabolismo , Fosfolipídeos/farmacologia , Retículo Sarcoplasmático/metabolismo , Membranas Intracelulares/metabolismo , Fosfatidilcolinas/farmacologia , Relação Estrutura-AtividadeRESUMO
Concentrations of concanavalin A or the calcium ionophore A23187 that are optimal for the transformation of pig or mouse lymphocytes do not normally cause a measurable increase in calcium influx compared with unstimulated cells. If the cells are treated with the mitogens in conditions where a measurable increase in calcium influx occurs, no stimulation of the cells can occur while the flux is maintained. If an early influx of extracellular calcium is necessary for stimulation, then a much smaller increase in the total concentration of cellular calcium than reported previously is sufficient to allow the entry of lymphocytes into the cell cycle.
Assuntos
Cálcio/metabolismo , Ativação Linfocitária , Linfócitos/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Calcimicina/farmacologia , Membrana Celular/efeitos dos fármacos , Células Cultivadas , Concanavalina A/farmacologia , DNA/biossíntese , Linfonodos , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/imunologia , Linfócitos/ultraestrutura , Macrófagos/imunologia , Camundongos , Mitose/efeitos dos fármacos , Baço , Suínos , Fatores de TempoAssuntos
Adenilil Ciclases/metabolismo , Glucagon/metabolismo , Receptores de Superfície Celular/metabolismo , Animais , Membrana Celular/metabolismo , Membrana Celular/efeitos da radiação , Elétrons , Fluoretos/farmacologia , Liofilização , Glucagon/farmacologia , Fígado/metabolismo , Masculino , Modelos Biológicos , RatosRESUMO
Pure complexes of dipalmitoyllecithin (DPL, 16:0) which Ca2+, Mg2+ dependent ATPase from sarcoplasmic reticulum are unusual in retaining significant ATPase activity down to about 30 degrees C, well below the transition temperature of the pure lipid at 41 degrees C. A minimum of about 35 lipid molecules per ATPase is required to maintain maximal ATPase activity, but the complexes are progressively and irreversibly inactivated at lower lipid to protein ratios. Complexes containing more than the minimum lipid requirement show very similar temperature profiles of activity about 30 degrees C over a wide range of lipid to protein ratios, up to 1500:1. Spin-label studies indicate that, at lipid to protein ratios of less than about 30 lipids per ATPase, no DPL phase transition can be detected, but at all higher ratios, a phase transition occurs at about 41 degrees C. In all of these complexes there are breaks in the Arrhenius plots of ATPase activity at 27--32 degrees C and at 37.5--38.5 degrees C. Experiments with perturbing agents, such as cholesterol and benzyl alcohol which have well-defined effects on the DPL phase transition, indicate that these breaks in the Arrhenius plots of ATPase activity cannot be attributed to a depressed and broadened phase transition in the lipids near the protein molecules. These results are interpreted as evidence for a phospholipid annulus of at least 30 lipid molecules with interact directly with the ATPase and cannot undergo a phase transition at 41 degrees C. This structural interaction of the ATPase with the annular DPL molecules has a predominant effect in determining the form of the temperature-activity profiles. However, the perturbation of the DPL phase transition does not extend significantly beyond the annulus since a phase transition which starts at 41 degrees C can be detected as soon as extraannular lipid is present in the complexes. We suggest that it may be a general feature of membrane structure that penetrant membrane proteins interact with their immediate lipid environment so as to cause only a minimal perturbation of the lipid bilayer.
Assuntos
Adenosina Trifosfatases/metabolismo , Cálcio/metabolismo , Proteínas Musculares/metabolismo , Retículo Sarcoplasmático/metabolismo , Animais , Álcoois Benzílicos , Sítios de Ligação , Transporte Biológico Ativo , Colesterol , Espectroscopia de Ressonância de Spin Eletrônica , Conformação Molecular , Ligação Proteica , Conformação Proteica , Coelhos , Ácidos Esteáricos , TemperaturaRESUMO
1. Activation of adenylate cyclase in rat liver plasma membranes by fluoride or GMP-P (NH)P yielded linear Arrheniun plots. Activation by glucagon alone, or in combination with either fluoride or GMP-P(NH)P resulted in biphasic Arrhenius plots with a well-defined break at 28.5 +/- 1 degrees C. 2. The competitive glucagon antagonist, des-His-glucagon did not activate the adenylate cyclase but produced biphasic Arrhenius plots in combination with fluoride or GMP-P(NH)P. The break temperatures and activation energies were very similar to those observed with glucagon alone, or in combination with either fluoride or GMP-P(NH)P. 3. It is concluded that although des-His-glucagon is a potent antagonist of glucagon, it nevertheless causes a structural coupling between the receptor and the catalytic unit.
Assuntos
Adenilil Ciclases/metabolismo , Membrana Celular/metabolismo , Glucagon/metabolismo , Fígado/metabolismo , Receptores de Superfície Celular , Animais , Sítios de Ligação , Ligação Competitiva , Ativação Enzimática/efeitos dos fármacos , Fluoretos/farmacologia , Glucagon/farmacologia , Nucleotídeos de Guanina/farmacologia , Cinética , Ligação Proteica , Ratos , Temperatura , TermodinâmicaRESUMO
1. The lipids composition of rat liver plasma membranes was substantially altered by introducing synthetic phosphatidylcholines into the membrane by the techniques of lipid substitution or lipid fusion. 40-60% of the total lipid pool in the modified membranes consisted of a synthetic phosphatidylcholine. 2. Lipid substitution, using cholate to equilibrate the lipid pools, resulted in the irreversible loss of a major part of the adenylate cyclase activity stimulated by F-, GMP-P(NH)P or glucagon. However, fusion with presonicated vesicles of the synethic phosphatidylcholines causes only small losses in adenylate cyclase activity stimulated by the same ligands. 3. The linear form of the Arrhenius plots of adenylate cyclase activity stimulated by F- or GMP-(NH)P was unaltered in all of the membrane preparations modified by substitution or fusion, with very similar activation energies to those observed with the native membrane. The activity of the enzyme therefore appears to be very insensitive to its lipid environment when stimulated by F- or gmp-p(nh)p. 4. in contrast, the break at 28.5 degrees C in the Arrhenius plot of adenylate cyclase activity stimulated by glucagon in the native membrane, was shifted upwards by dipalmitoyl phosphatidylcholine, downwards by dimyristoyl phosphatidylcholine, and was abolished by dioleoyl phosphatidylcholine. Very similar shifts in the break point were observed for stimulation by glucagon or des-His-glucagon in combination with F- or GMP-P(NH)P. The break temperatures and activation energies for adenylate cyclase activity were the same in complexes prepared with a phosphatidylcholine by fusion or substitution. 5. The breaks in the Arrhenius plots of adenylate cyclase activity are attributed to lipid phase separations which are shifted in the modified membranes according to the transition temperature of the synthetic phosphatidylcholine. Coupling the receptor to the enzyme by glucagon or des-His-glucagon renders the enzyme sensitive to the lipid environment of the receptor. Spin-label experiments support this interpretation and suggest that the lipid phase separation at 28.5 degrees C in the native membrane may only occur in one half of the bilayer.