Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 9 de 9
Filtrar
Mais filtros








Base de dados
Intervalo de ano de publicação
1.
Clin Radiol ; 79(7): e941-e948, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38710602

RESUMO

OBJECTIVES: The popularity of trans-radial access (TRA) for cerebral angiography is growing. Potential benefits of TRA over traditional trans-femoral access (TFA) are multitude. This study aimed to evaluate discharge outcomes and patient opinion of TRA compared to TFA in patients undergoing cerebral angiography prior to stereotactic radiosurgery (SRS) treatment for cerebral arteriovenous malformations. METHODS: Consecutive patients treated at the National Centre for Stereotactic Radiosurgery (Sheffield, United Kingdom) over a 22-month period were included. All patients underwent cerebral angiography with either TRA or TFA as part of treatment planning prior to SRS. TRA patients who had previously undergone TFA in other centres were surveyed for their experience of cerebral angiography using a questionnaire. SRS staff at our centre was approached for their opinion. RESULTS: 492 patients were included (median age = 43 years, 57.5% male, median lesions treated = 1). More patients underwent angiography with TFA (75.2%) than TRA (24.8%). No difference was found in accumulated dose for angiography between the groups (p>0.05). There was 17.6% reduction in overnight stay between TRA and TRF, the proportion of patients requiring overnight admission was higher for the TFA (35.2%) than TRA (17.6%, p<0.05). 101 patients were surveyed, with a response rate of 47%. Most respondents (79%) indicated preference for TRA over TFA. CONCLUSIONS: Use of TRA in pre-SRS cerebral angiography is feasible and improves both patient and staff experience. The adoption of TRA could have important implications for department resources and costs by reducing the proportion of overnight admissions.


Assuntos
Angiografia Cerebral , Malformações Arteriovenosas Intracranianas , Radiocirurgia , Humanos , Radiocirurgia/métodos , Masculino , Feminino , Malformações Arteriovenosas Intracranianas/diagnóstico por imagem , Malformações Arteriovenosas Intracranianas/cirurgia , Adulto , Angiografia Cerebral/métodos , Artéria Radial/diagnóstico por imagem , Pessoa de Meia-Idade , Resultado do Tratamento , Inquéritos e Questionários , Idoso , Adolescente , Artéria Femoral/diagnóstico por imagem , Estudos Retrospectivos
2.
J Biomed Mater Res A ; 83(2): 391-400, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17450583

RESUMO

Polypyrrole (PPy) is a conducting polymer that may be electrochemically generated with the incorporation of any anionic species, including net-negatively charged biological molecules such as proteins and polysaccharides. In this article, dermatan and chloride-loaded PPy films were prepared on gold sputter-coated coverslips and various skin derived cells were studied on them by electrochemical impedance spectroscopy. Impedance spectra in the frequency range 1-100 kHz were either determined at specific times or impedance was monitored continuously at specific frequencies. An equivalent impedance circuit was fitted to the recorded impedance spectra to obtain parameters whose contributions could be mapped to intracellular and intercellular current pathways, and the membrane properties of cells. Results show cell-induced impedance changes were detected over PPy modified electrodes and were dependent on cell density and type, monitoring frequency, material composition, and treatment. Lower cell densities were detected on PPy when compared with bare gold. Keratinocyte confluence, as determined by impedimetric analysis, was reached more rapidly on PPy than on gold. This was consistent with previous, more cumbersome, biochemical assays. Electrical equivalent circuit analysis provided evidence that the technique may be extended to discriminate cell type because of the intracellular and intercellular resistance, and cell membrane capacitance being related to cell morphology.


Assuntos
Técnicas Biossensoriais/métodos , Polímeros/metabolismo , Pirróis/metabolismo , Linhagem Celular , Impedância Elétrica , Ouro , Humanos , Queratinócitos/citologia , Queratinócitos/metabolismo , Polímeros/química , Pirróis/química , Fatores de Tempo
3.
In Vitro Cell Dev Biol Anim ; 41(7): 185-7, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16223332

RESUMO

Microarray technology has burgeoned over the past few years from nucleic acid-based arrays to tissue microarrays (TMAs). This study aimed to develop a technique to incorporate cell lines into an array and to demonstrate the usefulness of this technique by performing immunohistochemistry for beta-catenin. Cell suspensions were prepared from 23 tumor cell lines. These were fixed in formalin, suspended in agar, and embedded in paraffin to produce a cell block. A "tissue microarrayer" was used to remove triplicate, 0.6 mm-cores from each cell block and to transfer these into a recipient paraffin block at precise coordinates. Immunohistochemistry was used to identify cell lines positive for beta-catenin. Cultured cells were successfully incorporated into the microarray, with preservation of cell architecture and even distribution of cells within each core. A total of 18 of 69 cores (26%) were lost in processing. A total of 16 of 23 cell lines were identified as positive for membrane and cytoplasmic beta-catenin, and 6 of 23 were negative. Only one cell line was unscorable because of complete core loss. We have developed a "cell microarray" technique for analyzing antigen expression by immunohistochemistry in multiple cell lines in a single experiment. This novel application of microarrays permits high-throughput, cost-efficient analysis, with the potential to rapidly identify markers with potential diagnostic and therapeutic implications in human disease.


Assuntos
Linhagem Celular Tumoral/química , Imuno-Histoquímica/métodos , Técnicas de Diagnóstico Molecular , beta Catenina/análise , Biomarcadores Tumorais/análise , Linhagem Celular Tumoral/citologia , Técnicas Histológicas , Humanos
4.
Breast ; 13(3): 250-3, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15177432

RESUMO

Pyoderma gangrenosum rarely occurs in the breast and has only previously been reported in the literature in response to trauma. We describe a case occurring spontaneously in the breast that was initially mistaken for an infection.


Assuntos
Doenças Mamárias/diagnóstico , Pioderma Gangrenoso/diagnóstico , Doenças Mamárias/patologia , Doenças Mamárias/cirurgia , Diagnóstico Diferencial , Feminino , Humanos , Pessoa de Meia-Idade , Pioderma Gangrenoso/patologia , Pioderma Gangrenoso/cirurgia
5.
Int J Oncol ; 25(1): 167-71, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15202002

RESUMO

For well over a century, pathologists have used formalin-fixed paraffin-embedded tissues for microscopic examination and subsequent diagnosis of human disease. However, this type of approach is usually limited to the analysis and/or detection of one or two specific features on a single slide from an individual section. This area of histological study has been revolutionised by the development of tissue microarrays (TMAs). This review describes the development and use of TMAs and discusses their possible applications to clinical oncological practice.


Assuntos
Neoplasias/genética , Análise de Sequência com Séries de Oligonucleotídeos , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Feminino , Humanos , Neoplasias/patologia , Reprodutibilidade dos Testes
6.
J Vasc Surg ; 33(4): 874-9, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11296345

RESUMO

Aortoenteric fistulation (AEF) is a well-documented late complication of open abdominal aortic aneurysm (AAA) repair, occurring in between 0.4% and 4% of cases. In the absence of an anastomosis, AEF is likely to be rare after endovascular aneurysm repair (EVAR) and has only recently been described in the literature as a result of mechanical stent failure or migration. We present the case of a 61-year-old man who underwent EVAR for an AAA with a "nonspecific" periaortic inflammatory mass. Six months postoperatively, an AEF developed, presenting with metastatic sepsis followed by septic infective thromboembolization to his right leg, and amputation was necessary. His stent was well positioned and mechanically intact. We emphasize the need for vigilance about the risk of AEF when adopting an endovascular approach to repair the AAA with a nonspecific periaortic inflammatory mass and highlight the need for awareness about the unusual septic manifestations of AEF.


Assuntos
Aneurisma da Aorta Abdominal/terapia , Doenças da Aorta/etiologia , Duodenopatias/etiologia , Fístula Intestinal/etiologia , Stents/efeitos adversos , Fístula Vascular/etiologia , Aneurisma da Aorta Abdominal/complicações , Aneurisma da Aorta Abdominal/diagnóstico por imagem , Aneurisma da Aorta Abdominal/patologia , Doenças da Aorta/diagnóstico por imagem , Duodenopatias/diagnóstico por imagem , Infecções por Bactérias Gram-Positivas/diagnóstico , Infecções por Bactérias Gram-Positivas/cirurgia , Humanos , Inflamação , Fístula Intestinal/diagnóstico por imagem , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/cirurgia , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Infecções Relacionadas à Prótese/diagnóstico , Infecções Relacionadas à Prótese/cirurgia , Tomografia Computadorizada por Raios X , Fístula Vascular/diagnóstico por imagem
7.
Physiol Meas ; 22(1): 49-54, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11236889

RESUMO

This paper describes the Sheffield Mk3.5 EIT/EIS system which measures both the real and imaginary part of impedance at 30 frequencies between 2 kHz and 1.6 MHz. The system uses eight electrodes with an adjacent drive/receive electrode data acquisition protocol. The system is modular, containing eight identical data acquisition boards, which contain DSPs to generate the drive frequencies and to perform the FFT used for demodulation. The current drive is in three sequentially applied packets, where each packet contains ten summed sine waves. The data acquisition system is interfaced to a host PC through an optically isolated high speed serial link (RS485) running at 2 Mbaud (2 Mbits s(-1)). Measurements on a saline filled tank show that the average signal to noise performance of the system is 40 dB measured across all frequencies and that this figure is independent of frequency of measurement. These results suggest that the current system is 10 dB better in absolute terms than the previous Sheffield (Mk3a) system.


Assuntos
Impedância Elétrica , Tomografia/instrumentação , Eletrodos , Desenho de Equipamento , Humanos , Processamento de Sinais Assistido por Computador , Software , Tomografia/estatística & dados numéricos
9.
Physiol Meas ; 21(1): 137-44, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10720009

RESUMO

Electrical impedance measurements are used to obtain information about a subject, tissue sample or tissue model under test. There are several ways of obtaining these impedance data and thereafter analysing the data to obtain relevant parameters. This paper shows how a completely isolated drive and receive system using current pulses, as opposed to sine waves, achieves good fitted results with resistor-capacitor Cole phantoms.


Assuntos
Impedância Elétrica , Tomografia/métodos , Tomografia/estatística & dados numéricos , Interpretação Estatística de Dados , Humanos , Modelos Estatísticos , Tomografia/instrumentação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA