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1.
Am J Community Psychol ; 72(3-4): 249-253, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37957837

RESUMO

When Seymour Sarason, the founder of American community psychology, looked back on his life and work, he singled out the importance of personal humility and of developing collaborative learning relationships. He worried that humility was too lacking in psychology. To cultivate humility, we need to engage in an ongoing practice of critical self- and group-examination that enables us to understand more fully the effects of our positionalities, historical, and cultural contexts. Alongside this we need to try to understand the ecopsychosocial and historical contexts of those we have been invited to accompany. For those who are European descended, this requires a deepening realization of how we, as W. E. B. Du Bois would say, have been and are a "problem." Unawares, we have saturated psychology with our own cultural perspectives and ways of being. "White" people require their own pedagogy to become more conscious of their standpoints and to redress the harms created by their group. Our task is not to evangelize psychological theories and practices born from within our own particular cultural perspective, but to learn from the cultural workers and community members in the group we are working with. We must ask of ourselves questions that enable us to understand the broader historical, social, and ecological context of the issues that are presenting. To indicate this, I preface the term "accompaniment" with the adjective "ecopsychosocial." Ecopsychosocial accompaniment requires humility. It is humility that opens the door to being able to imagine and desire together, to cocreate, and cosustain the kinds of decolonial spaces, places, and ways of working and living with one another that are so desperately needed.


Assuntos
Teoria Psicológica , Psicologia Social , Masculino , Humanos , Estados Unidos
3.
Med Phys ; 49(1): 129-143, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34748660

RESUMO

PURPOSE: Cardiovascular magnetic resonance (CMR) is a vital diagnostic tool in the management of cardiovascular diseases. The advent of advanced CMR technologies combined with artificial intelligence (AI) has the potential to simplify imaging, reduce image acquisition time without compromising image quality (IQ), and improve magnetic field uniformity. Here, we aim to implement two AI-based deep learning techniques for automatic slice alignment and cardiac shimming and evaluate their performance in clinical cardiac magnetic resonance imaging (MRI). METHODS: Two deep neural networks were developed, trained, and validated on pre-acquired cardiac MRI datasets (>500 subjects) to achieve automatic slice planning and shimming (implemented in the scanner) for CMR. To examine the performance of our automated cardiac planning (EasyScan) and AI-based shim (AI shim), two prospective studies were performed subsequently. For the EasyScan validation, 10 healthy subjects underwent two identical CMR protocols: with manual cardiac planning and with AI-based EasyScan to assess protocol scan time difference and accuracy of cardiac plane prescriptions on a 1.5 T clinical MRI scanner. For the AI shim validation, a total of 20 subjects were recruited: 10 healthy and 10 cardio-oncology patients with referrals for a CMR examination. Cine images were obtained with standard cardiac volume shim and with AI shim to assess signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), overall IQ (sharpness and MR image degradation), ejection fraction (EF), and absolute wall thickening. A hybrid statistical method using of nonparametric (Wilcoxon) and parametric (t-test) assessments was employed for statistical analyses. RESULTS: CMR protocol with AI-based plane prescriptions, EasyScan, minimized operator dependence and reduced overall scanning time by over 2 min (∼13 % faster, p < 0.001) compared to the protocol with manual cardiac planning. EasyScan plane prescriptions also demonstrated more accurate (less plane angulation errors from planes manually prescribed by a certified cardiac MRI technologist) cardiac planes than previously reported strategies. Additionally, AI shim resulted in improved B0 field homogeneity. Cine images obtained with AI shim revealed a significantly higher SNR (12.49%; p = 0.002) than those obtained with volume shim (volume shim: 32.90 ± 7.42 vs. AI shim: 37.01 ± 8.87) for the left ventricle (LV) myocardium. LV myocardium CNR was 12.48% higher for cine imaging with AI shim (149.02 ± 39.15) than volume shim (132.49 ± 33.94). Images obtained with AI shim resulted in sharper images than those obtained with volume shim (p = 0.012). The LVEF and absolute wall thickening also showed that differences exist between the two shimming methods. The LVEF by AI shim was shown to be slightly larger than LVEF by volume shim in two groups: 2.87% higher with AI shim for the healthy group and 1.70% higher with AI shim for the patient group. The LV absolute wall thickening (in mm) also showed that differences exist between shimming methods for each group with larger changes observed in the patient group (healthy: 3.31%, p = 0.234 and patient group: 7.29%, p = 0.059). CONCLUSIONS: CMR exams using EasyScan for cardiac planning demonstrated accelerated cardiac exam compared to the CMR protocol with manual cardiac planning. Improved and more uniform B0 magnetic field homogeneity also achieved using AI shim technique compared to volume shimming.


Assuntos
Inteligência Artificial , Imageamento por Ressonância Magnética , Coração/diagnóstico por imagem , Humanos , Imagem Cinética por Ressonância Magnética , Estudos Prospectivos , Reprodutibilidade dos Testes , Volume Sistólico
4.
Am J Community Psychol ; 62(3-4): 319-329, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30552690

RESUMO

Community psychology's history has traditionally been described within the context of U.S. history, silencing contributions from people of color from the Americas, Asia, the Pacific Islands, and Africa. In a MA/PhD specialization in Community Psychology, Liberation Psychology, Indigenous Psychologies, and Ecopsychology at Pacifica Graduate Institute, we are attempting to steer into critical dialogues about modernity, coloniality, and decoloniality, closely examining our curriculum and pedagogy, including our approaches to fieldwork and research. Turning to Indigenous psychologists, decolonial and critical race theorists, and cultural workers within the U.S. and from the Global South, we are attempting to challenge coloniality in the social sciences, community psychology, and in our own thinking and teaching to unmask hegemonic assumptions and open space for decolonial theory and practice. In this paper, we explore ways in which we are working with our graduate students and faculty to co-construct a decolonial curriculum that integrates decoloniality so that knowledges from historically silenced locations, as well as anti-racist and other decolonial praxes can co-exist and thrive.


Assuntos
Colonialismo , Psicologia Social/educação , Ensino , Cultura , Currículo , Justiça Social , Estados Unidos
5.
Int J Nurs Stud ; 78: 76-83, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29110907

RESUMO

BACKGROUND: Nursing is an integral part of all healthcare services, and has the potential of having a wide and enduring impact on health outcomes for a global ageing population. Over time nurses have developed new roles and assumed greater responsibilities. It is increasingly important to demonstrate the safety and overall impact of nurses' practice through research, to support the case for greater investment and development of nursing services around the world. OBJECTIVE: To provide an overview of existing research evidence on the impact of nursing on patient outcomes, identify gaps in evidence, and point to future priorities for global research. Specifically to address two questions: what is the evidence that nursing contributes to improving the health and well-being of populations?; and where should research activity be focused to strengthen the evidence base for the impact of nursing? METHODS: A search of the literature from 1996 using CINAHL, MEDLINE, the Cochrane Library, Google Scholar and the NICE evidence databases using the key words: nursing, nurse led, nursing interventions and patient outcomes. Initial analysis of the retrieved citations to reveal clusters of evidence of nursing impact in clinical areas which had been subject to systematic/integrative reviews or meta-analyses. Further analysis of these reviews to provide an overview of the research evidence for nurses' contributions to healthcare to inform discussion on future research agendas. We use the terms low, moderate and high quality evidence to reflect the assessments made by the review authors whose work is presented throughout. RESULTS: Analysis of 61 reviews, including ten Cochrane reviews and two scoping/selective reviews to provide a summary of the research evidence for nurses' contributions to healthcare in the following areas of practice: nursing in acute care settings; nurses' involvement in public health; the contribution of specialist nurse and nurse-led services to the management of chronic disease; comparison of care provided by nurses and doctors; and task shifting to invasive procedures. CONCLUSIONS: There is evidence that adequate numbers of well-educated nurses working in acute care areas can reduce the risk of patient mortality, although the evidence for this is confined to studies in high income countries and the evidence is not sufficiently robust to draw up definitive nurse: patient ratios. There is also moderate evidence that well trained nurses can produce health outcomes that are equivalent to those of doctors for patients with a range of chronic health problems, particularly for those patients managed in primary care, and that nurse-led care may be more effective than medical care in promoting patient adherence to treatment and patient satisfaction. There is low to moderate evidence for the benefits of parenting support programmes delivered by nurses on a range of health outcomes; and for the impact of home visiting on improving function and other health service outcomes for older people. The wider societal benefits of home visiting by nurses and the impact of this on long term outcomes and related cost-effectiveness of home visiting has not been established. There is limited available information regarding the wider global impact of increasing the numbers of nurses and their contribution to healthcare through improved education. Moreover there is very little evidence for the cost-effectiveness of changing care providers from doctors to nurses and as the majority of cost data available has tended to come from studies based in higher income countries, their external validity in terms of applicability to settings in low and middle income countries is questionable. In addition to effectiveness, cost and safety, future research needs to address how implementing expanded nursing roles and task shifting impacts on the morale, retention, and professional development of nurses and the other workforces, and the longer term implications of these developments both locally and internationally.


Assuntos
Enfermagem Geriátrica , Idoso , Humanos , Resultado do Tratamento
6.
Cardiol Res ; 5(2): 83-84, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26191115

RESUMO

Bicuspid pulmonary valves and pulmonary artery aneurysms are two rare entities, reported in association, and usually attributed to hemodynamic alterations caused by the bicuspid pulmonary valve. We present magnetic resonance images of a patient with a bicuspid pulmonary valve and pulmonary artery aneurysm, and propose an alternative mechanism for this association, based on recent embryologic studies that link anomalies of the semilunar valves and great vessels with derangement of the cardiac neural crest cell development.

8.
Int J Health Care Qual Assur ; 24(2): 164-77, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21456491

RESUMO

PURPOSE: The purpose of this paper is to describe learning gained from involving service users and carers as researchers in an action research study. The researchers aimed to introduce self-directed support in mental health services--part of the government's personalisation agenda, which requires a move from current social care commissioning, where a person is matched to available services, to one where a person self-assesses, has an agreed support plan and then with appropriate help, purchases his or her own services to lead as independent a life as possible. This development is allied closely with the mental health service recovery approach. DESIGN/METHODOLOGY/APPROACH: Three service users and two carers were recruited to work alongside the lead researcher. Service users were fully involved in the steering group - part of participatory action research project. Data were collected from: debriefing meetings with co-researchers between April and December 2007; a group interview held by the lead researcher; and participants' journal comments and self-reflections. FINDINGS: The main areas in which service users and carers found involvement difficult were: overcoming professional language barriers; emotional impact; and power imbalances between themselves and professionals. Findings suggest that considerable improvement is required by mental health professionals and managers if service users and carers are successfully involved in projects. RESEARCH LIMITATIONS/IMPLICATIONS: This is a small study within a larger action research project. Findings are not generalisable owing to the small sample; however, findings are supported by the service-user involvement literature. ORIGINALITY/VALUE: Few studies explore participation effects on service users and carers from their perspective. This research provides insights into what needs to be addressed to improve service user and carer involvement to improve mental health services.


Assuntos
Cuidadores , Emoções , Serviços de Saúde Mental/organização & administração , Pacientes , Qualidade da Assistência à Saúde/organização & administração , Comunicação , Comportamento Cooperativo , Humanos , Participação do Paciente , Relações Profissional-Paciente
9.
J Clin Nurs ; 19(17-18): 2565-73, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20546385

RESUMO

AIMS: To construct and test an activity diary designed to measure the impact and explore the activities of nurse and allied health professional consultants in relation to each speciality and function of the role. BACKGROUND: This was part of a funded feasibility study to assess the contribution of nurse and allied health professional consultants. DESIGN: This was an exploratory study. METHOD: Thematic analysis of guided discussions with five nurse consultants and one physiotherapy consultant identified activities which were used to construct an activity diary. The activities were grouped under the four pillars or functions of the consultant role; expert practice, leadership, research and education. Participants recorded their activities in a diary over a one-week period. CONCLUSION: Results suggest that with some modification, this activity diary could be used to capture the impact, complexity and diversity of activities of the consultant role. RELEVANCE TO PRACTICE: Advanced practice roles are essential to the healthcare workforce of the future. This tool provides a method for measuring the contribution and complexity of the consultant role.


Assuntos
Pessoal Técnico de Saúde , Enfermeiras e Enfermeiros , Encaminhamento e Consulta/normas , Humanos , Liderança , Inquéritos e Questionários , Reino Unido
10.
J Natl Black Nurses Assoc ; 20(2): 38-41, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20364725

RESUMO

This study was a follow-up to a previous study that was done among first semester nursing college students at a historically Black college and university in northeastern United States. The original intent was to ascertain their perceptions and knowledge of various aspects of the Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome (HIV/AIDS) phenomenon. It involved an anonymous survey questionnaire featuring yes and no answers, a Likert scale, and qualitative response questions concerning prevention behavior. Respondents were asked whether HIV/AIDS could make an individual "sick enough to die", whether they thought that wearing a condom would diminish their feeling during the sexual experience, and whether they would insist their partners use a condom. The original study consisted of 68 students. The follow-up study,four years later, consisted of the 20 remaining nursing students from the original cohort and was undertaken with the intent to appraise their knowledge level and to ascertain whether their attitudes towards the use of condoms as a way to prevent HIV/AIDS and STDs had changed. Findings from this follow-up study indicated that educational attainment of the respondents did not translate into a change in attitudes about responsible sexual behavior. Further findings may suggest that intensive HIV/AIDS education begin at an earlier phase in a students'education. Along with the basics of HIV/AIDS education, students need to learn to be respectful of each others' health and wellbeing. It is essential that a nurturing and protective environment exist so that young women are not afraid of the consequences related to asking partners to wear condoms during sexual encounters.


Assuntos
Atitude Frente a Saúde/etnologia , Negro ou Afro-Americano/educação , Negro ou Afro-Americano/etnologia , Infecções por HIV/prevenção & controle , Conhecimentos, Atitudes e Prática em Saúde , Estudantes de Enfermagem/psicologia , Negro ou Afro-Americano/estatística & dados numéricos , Preservativos , Bacharelado em Enfermagem/organização & administração , Feminino , Seguimentos , Infecções por HIV/epidemiologia , Infecções por HIV/transmissão , Humanos , New England/epidemiologia , Pesquisa Metodológica em Enfermagem , Pesquisa Qualitativa , Sexo Seguro/etnologia , Sexo Seguro/estatística & dados numéricos , Parceiros Sexuais , Estudantes de Enfermagem/estatística & dados numéricos , Inquéritos e Questionários , Universidades , Adulto Jovem
11.
J Nurs Manag ; 16(6): 707-15, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18808465

RESUMO

OBJECTIVES: To differentiate between English NHS trust board meetings according to the percentage of clinical content and to explore which characteristics of board meetings might explain this. METHODS: Definition of scoring system for clinical content. Scoring of minutes for a random sample of 60 trusts. Qualitative analysis of a sub-sample, generated hypotheses about factors leading to higher percentage of clinical items was undertaken; testing of hypotheses in a longitudinal sample of minutes from 24 trusts over 1 year. RESULTS: Clinical content varied from 2% to 30%. Boards with a more clinical focus tended to link other issues including finance to clinical issues; have non-executive directors able to question board executives openly; make less use of acronyms in minutes; had more liaison with social services; and accepted questions from the public. CONCLUSIONS: Counting items in board minutes has prima facie validity as a means of defining how clinically focussed board meetings are, although more research is required to refine the method. The present method of analysing board minutes may provide one way of assessing board culture. IMPLICATIONS FOR NURSING MANAGEMENT: Directors of nursing can help focus trust board meetings on clinical matters. Further research is required to determine whether greater clinical content in trust board meetings has impacts on clinical practice or organizational performance.


Assuntos
Governança Clínica/organização & administração , Tomada de Decisões Gerenciais , Conselho Diretor/organização & administração , Medicina Estatal/organização & administração , Atitude do Pessoal de Saúde , Estudos Transversais , Coleta de Dados , Inglaterra , Conhecimentos, Atitudes e Prática em Saúde , Pesquisa sobre Serviços de Saúde , Humanos , Estudos Longitudinais , Enfermeiros Administradores/organização & administração , Enfermeiros Administradores/psicologia , Papel do Profissional de Enfermagem , Pesquisa Metodológica em Enfermagem , Cultura Organizacional , Objetivos Organizacionais , Formulação de Políticas , Pesquisa Qualitativa , Estudos de Tempo e Movimento
12.
Open Nurs J ; 2: 8-14, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19319215

RESUMO

Advanced practice roles in nursing and other health professions have become integral to a range of healthcare services world-wide providing experienced practitioners with the opportunity to extend their roles and influence decision making whilst maintaining patient contact. However, there has been limited research to evaluate the impact on health services of these advanced practice roles.The aim of this feasibility study was to analyse Nurse and Allied Health Professionals (AHP) Consultant activity in the four functions of the role (education, expert practice, leadership, research). A structured diary assessed the activity of five Consultant Nurses and one Consultant Physiotherapist over a one week period. Detailed analysis of a clinic carried out by one of the Consultants was undertaken using a mapping procedure.Results suggest that the activity diary, combined with mapping of activities, illustrate the complexity and variety of the role and facilitate meaningful reflection on what advanced practitioners do.

13.
J Clin Nurs ; 16(10): 1792-808, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17880468

RESUMO

AIM: This paper presents the findings of a systematic review and meta-synthesis, which was undertaken to identify and assess studies that evaluated the nurse/midwife/allied health professional consultant role. BACKGROUND: As part of the modernization agenda in the National Health Service the United Kingdom government proposed 'Consultant' posts for nurses/midwives and allied health professionals as an opportunity for experienced practitioners to extend their roles. Four key functions were identified as being significant to this role development: expert practice, leadership, education and research. Explicit within the proposal was a requirement of these new roles to be evaluated. METHOD: Inclusion criteria focussed on studies where an aspect of the role had been evaluated or where the consultant carried out the research. A total of 1931 citations was filtered to reveal 107 studies that possibly met the inclusion criteria. Of these 107, 14 studies were critically appraised and subjected to thematic analysis. Study methodology varied but of the 14 studies, 10 involved some form of measurement. The others adopted a literature review or descriptive approach. CONCLUSIONS: The extent to which consultants were involved in all aspects of the 'four pillars' was assessed in only a small number of studies. However, a number of studies implicitly implied active engagement in expert practice and leadership by focussing on specific service developments. RELEVANCE TO CLINICAL PRACTICE: To date, a number of studies evaluating service developments, education, expert practice and leadership have been carried out. However although a number of studies have assessed perceived impact of the consultant role, no measure of actual benefit has been published to date. Studies that evaluate the cost benefit/outcomes of these roles in relation to both activity and quality of service are required.


Assuntos
Pessoal Técnico de Saúde/organização & administração , Consultores , Enfermeiros Clínicos/organização & administração , Enfermeiros Obstétricos/organização & administração , Papel do Profissional de Enfermagem , Pesquisa em Avaliação de Enfermagem/organização & administração , Pessoal Técnico de Saúde/educação , Coleta de Dados , Interpretação Estatística de Dados , Humanos , Liderança , Enfermeiros Clínicos/educação , Enfermeiros Obstétricos/educação , Autonomia Profissional , Competência Profissional , Projetos de Pesquisa , Pesquisadores/educação , Pesquisadores/organização & administração
14.
J Natl Black Nurses Assoc ; 18(2): 24-9, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18318328

RESUMO

There are always some assumptions that are made about the knowledge of the Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome (HIV/AIDS) phenomenon among graduating high school seniors. To ascertain their knowledge, a categorical survey questionnaire was designed featuring yes and no answers, a Likert scale, and qualitative response questions to the problem of HIV/AIDS prevention behavior. It was administered to first semester nursing college students at an historically black college and university (HBCU) in northeastern United States. These freshman students were enrolled in the course University Seminar. The class consisted of 68 students (64 females and 4 males). The students in this class anonymously completed the questionnaire about their perceptions and knowledge of the HIV/AIDS phenomenon. Findings from the study indicated that the majority of the students knew that HIV/AIDS could make an individual sick enough to die. The students stated, however, that they were willing to submit to the sexual demands of their partners, even when they refused to wear condoms. Of the 64 female students who responded to the questionnaire, 38 stated that using a condom might diminish their feeling during the sexual experience and they thought that it was very important that that did not happen. Therefore, this situation might affect a student's decision as to whether or not to insist on using a condom. In conclusion, it is recommended that intensive HIV/AIDS education be incorporated into the curriculum and the questionnaire be repeated in these students' junior and senior years to ascertain whether or not there was a change in their attitudes.


Assuntos
Atitude Frente a Saúde/etnologia , Negro ou Afro-Americano/etnologia , Infecções por HIV/prevenção & controle , Conhecimentos, Atitudes e Prática em Saúde , Estudantes de Enfermagem/psicologia , Universidades , Adolescente , Adulto , Negro ou Afro-Americano/educação , Preservativos/estatística & dados numéricos , Tomada de Decisões , Bacharelado em Enfermagem , Feminino , Infecções por HIV/epidemiologia , Infecções por HIV/transmissão , Necessidades e Demandas de Serviços de Saúde , Humanos , Masculino , New England , Pesquisa em Educação em Enfermagem , Pesquisa Metodológica em Enfermagem , Pesquisa Qualitativa , Fatores de Risco , Sexo Seguro/etnologia , Educação Sexual , Inquéritos e Questionários
16.
Radiol Manage ; 28(4): 32-40; quiz 41-3, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16922124

RESUMO

According to the American Registry of Radiologic Technologists (ARRT), the current shortage of radiologic technologists (RTs) will continue to grow and by 2010, when many RTs are expected to retire from the profession, there will be an estimated 24,000 vacancies. In response to the shortage of RTs, The MGH Institute of Health Professions has developed a fast-track hybrid online graduate program in medical imaging (MI) which is geared toward bachelor degree prepared students who are seeking to make a career change. By targeting bachelor prepared students, the Institute seeks to attract academically mature students who will make a sustaining contribution to the field of radiologic science. The first class of students in this new online program just graduated. Measured results of this first group are very encouraging with all results exceeding the goals initially set for the program. Except for one student who has visa issues precluding employment, all of the students from the class have passed their registry exam and are employed in the field. The format of this new online MI program eases constraints on growth and viability and it bridges the gap between entry-level and advanced practice. This hybrid online program is a model that could be implemented with healthcare systems nationwide.


Assuntos
Diagnóstico por Imagem , Internet , Tecnologia Radiológica/educação , Educação Continuada , Humanos , Desenvolvimento de Programas , Avaliação de Programas e Projetos de Saúde
17.
Am J Physiol Heart Circ Physiol ; 291(5): H2515-21, 2006 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16751290

RESUMO

Rats and genetically manipulated mouse models have played an important role in the exploration of molecular causes of cardiovascular diseases. However, it has not been fully investigated whether mice or rats and humans manifest similar patterns of ventricular wall motion. Although similarities in anatomy and myofiber architecture suggest that fundamental patterns of ventricular wall motion may be similar, the considerable differences in heart size, heart rate, and sarcomeric protein isoforms may yield quantitative differences in ventricular wall mechanics. To further our understanding of the basic mechanisms of myofiber contractile performance, we quantified regional and global indexes of ventricular wall motion in mice, rats, and men using magnetic resonance (MR) imaging. Both regular cine and tagged MR images at apical, midventricular, and basal levels were acquired from six male volunteers, six Fischer 344 rats, and seven C57BL/6 mice. Morphological parameters and ejection fraction were computed directly from cine images. Myocardial twist (rotation angle), torsion (net twist per unit length), circumferential strain, and normalized radial shortening were calculated by homogeneous strain analysis from tagged images. Our data show that ventricular twist was conserved among the three species, leading to a significantly smaller torsion, measured as net twist per unit length, in men. However, both circumferential strain and normalized radial shortening were the largest in male subjects. Although other parameters, such as circumferential-longitudinal shear strain, need to be evaluated, and the causes of these differences in contractile mechanics remain to be elucidated, the preservation of twist appears fundamental to cardiac function and should be considered in studies that extrapolate data from animals to humans.


Assuntos
Ventrículos do Coração/anatomia & histologia , Imagem Cinética por Ressonância Magnética , Movimento (Física) , Função Ventricular Esquerda/fisiologia , Função Ventricular , Adolescente , Animais , Criança , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Contração Miocárdica/fisiologia , Ratos , Ratos Endogâmicos F344
19.
Kidney Int ; 66(4): 1345-55, 2004 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15458427

RESUMO

BACKGROUND: PKHD1, the autosomal-recessive polycystic kidney disease (ARPKD) gene, encodes multiple alternatively spliced transcripts predicted to generate membrane-bound and secreted proteins. The longest open reading frame encodes polyductin (fibrocystin), a putative 4074 amino acid protein with a single transmembrane domain and an intracellular C-terminus. METHODS: To characterize the PKHD1 products and their expression profile, we raised polyclonal antibodies against different portions of polyductin and analyzed different organs using various methods. RESULTS: Western blot analyses demonstrated specific bands of >440 kD in human adult kidney, liver, and pancreas and approximately 230 kD in kidney and liver, predominantly observed in membrane fractions. The >440-kD putative membrane protein was immunoprecipitated from kidney and subsequently detected by Western blotting using two distinct antisera. An additional product of approximately 140 kD was specifically recognized by affinity-purified antisera predominantly in soluble fractions. Immunohistochemistry studies revealed specific staining in cortical and medullary collecting ducts and thick ascending limbs of Henle (TALH). Serial sections were stained with antibodies against aquaporin-2 and Tamm-Horsfall protein to confirm the nephron segment localization. Positive staining was also detected in biliary and pancreatic duct epithelia. Analyses of mouse developing tissues showed specific staining in the ureteric bud branches, intra- and extrahepatic biliary ducts, pancreatic ducts, and salivary glands. Immunofluorescence studies in inner medullary collecting duct cultured cells and immunoelectron microscopy analysis of medullary collecting ducts demonstrated that the protein localizes to the primary cilium. Positive signal was also detected in the apical membrane and in cytoplasm. CONCLUSION: The results indicate that polyductin is part of the group of polycystic kidney disease (PKD)-related proteins expressed in primary apical cilia. Our data also suggest that, in addition to its likely involvement in cilia function, polyductin probably serves in other subcellular functional roles. The detection of three different products using two antisera, with evidence for distinct subcellular localizations, suggests that PKHD1 encodes membrane-bound and soluble isoforms.


Assuntos
Membrana Celular/metabolismo , Cílios/metabolismo , Citoplasma/metabolismo , Túbulos Renais Coletores/metabolismo , Receptores de Superfície Celular/metabolismo , Adulto , Animais , Anticorpos , Linhagem Celular , Membrana Celular/ultraestrutura , Polaridade Celular/fisiologia , Cílios/ultraestrutura , Citoplasma/ultraestrutura , Humanos , Imuno-Histoquímica , Isomerismo , Túbulos Renais Coletores/citologia , Camundongos , Microscopia Imunoeletrônica , Receptores de Superfície Celular/química , Receptores de Superfície Celular/imunologia
20.
J Am Coll Cardiol ; 44(1): 133-7, 2004 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-15234421

RESUMO

OBJECTIVES: The purpose of this study was to evaluate the reliability of the pressure half-time (PHT) method for estimating mitral valve areas (MVAs) by velocity-encoded cardiovascular magnetic resonance (VE-CMR) and to compare the method with paired Doppler ultrasound. BACKGROUND: The pressure half-time Doppler echocardiography method is a practical technique for clinical evaluation of mitral stenosis. As CMR continues evolving as a routine clinical tool, its use for estimating MVA requires thorough evaluation. METHODS: Seventeen patients with mitral stenosis underwent echocardiography and CMR. Using VE-CMR, MVA was estimated by PHT method. Additionally, peak E and peak A velocities were defined. Interobserver repeatability of VE-CMR was evaluated. RESULTS: By Doppler, MVAs ranged from 0.87 to 4.49 cm2; by CMR, 0.91 to 2.70 cm2, correlating well between modalities (r = 0.86). The correlation coefficient for peak E and peak A between modalities was 0.81 and 0.89, respectively. Velocity-encoded CMR data analysis provided robust, repeatable estimates of peak E, peak A, and MVA (r = 0.99, 0.99, and 0.96, respectively). CONCLUSIONS: Velocity-encoded cardiovascular magnetic resonance can be used routinely as a robust tool to quantify MVA via mitral flow velocity analysis with PHT method.


Assuntos
Imageamento por Ressonância Magnética , Estenose da Valva Mitral/diagnóstico , Valva Mitral/diagnóstico por imagem , Ultrassonografia Doppler , Idoso , Idoso de 80 Anos ou mais , Ecocardiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valva Mitral/patologia , Estenose da Valva Mitral/epidemiologia , Variações Dependentes do Observador , Radiografia , Reprodutibilidade dos Testes , Índice de Gravidade de Doença , Estatística como Assunto
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