RESUMO
The increasing number of reports of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) world-wide attests to the public health concern surrounding this pathogen in animal husbandry and in-contact humans. In Europe, LA-MRSA CC398 is predominant and generally regarded as being of low virulence for animals. Herein we report the recovery of a lineage of LA-MRSA, belonging to CC30, from three pigs in Northern Ireland and which encodes a marker of virulence (lukM and lukF-P83) restricted to animal-associated clones of S. aureus.
Assuntos
Proteínas de Bactérias/metabolismo , Staphylococcus aureus Resistente à Meticilina/metabolismo , Infecções Estafilocócicas/veterinária , Doenças dos Suínos/microbiologia , Animais , Proteínas de Bactérias/genética , Regulação Bacteriana da Expressão Gênica , Staphylococcus aureus Resistente à Meticilina/genética , Irlanda do Norte/epidemiologia , Filogenia , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Suínos , Doenças dos Suínos/epidemiologiaRESUMO
APXIVA is an RTX toxin of Actinobacillus pleuropneumoniae that is a candidate antigen to differentiate infected from vaccinated animals (DIVA). Insertion of ISApl1 into the apxIVA gene is known to compromise an APXIVA-based DIVA approach, as is potentially a TGG to TGA mutation in the apxIVA gene. ISApl1 was found in 63/349 (18.1%) A. pleuropneumoniae isolates from England and Wales including serovars 2, 3, 6-8 and 12. No ISApl1 insertions into apxIVA were found. Only two serovar 3 isolates contained the TGG to TGA mutation. We conclude that an ApxIVA-based DIVA approach would potentially be viable in England and Wales.