Biochemical Characterization and Functional Analysis of Glucose Regulated Protein 78 from the Silkworm Bombyx mori.

The glucose regulated protein (GRP78) is an important chaperone for various environmental and physiological stimulations. Despite the importance of GRP78 in cell survival and tumor progression, the information regarding GRP78 in silkworm Bombyx mori L. is poorly explored. We previously identified that GRP78 expression was significantly upregulated in the silkworm Nd mutation proteome database. Herein, we characterized the GRP78 protein from silkworm B. mori (hereafter, BmGRP78). The identified BmGRP78 protein encoded a 658 amino acid residues protein with a predicted molecular weight of approximately 73 kDa and comprised of two structural domains, a nucleotide-binding domain (NBD) and a substrate-binding domain (SBD). BmGRP78 was ubiquitously expressed in all examined tissues and developmental stages by quantitative RT-PCR and Western blotting analysis. The purified recombinant BmGRP78 (rBmGRP78) exhibited ATPase activity and could inhibit the aggregating thermolabile model substrates. Heat-induction or Pb/Hg-exposure strongly stimulated the upregulation expression at the translation levels of BmGRP78 in BmN cells, whereas no significant change resulting from BmNPV infection was found. Additionally, heat, Pb, Hg, and BmNPV exposure resulted in the translocation of BmGRP78 into the nucleus. These results lay a foundation for the future identification of the molecular mechanisms related to GRP78 in silkworms.

Bibliometric review of 1992-2022 publications on acupuncture for cognitive impairment.

Objective: To explore the development context, research hotspots, and frontiers of acupuncture therapy for cognitive impairment (CI) from 1992 to 2022 by visualization analysis. Methods: Articles about acupuncture therapy for cognitive impairment were retrieved from the Web of Science Core Collection (WoSCC) until 1 March 2022. Basic information was collected by Excel 2007, and VOSviewer 1.6.17 was used to analyze the co-occurrence of countries, institutes, and authors. Co-citation maps of authors and references were analyzed by CiteSpace V.5.8.R3. In addition, CiteSpace was used to analyze keyword clusters and forecast research frontiers. Results: A total of 279 articles were retrieved, including articles from 19 countries, 334 research institutes, and 101 academic journals. The most published country and institutes were the People's Republic of China (217) and the Fujian University of Traditional Chinese Medicine (40). Ronald C Petersen owned the highest co-citations (56). Keywords and co-cited references cluster showed the main research directions in this area, including "ischemic stroke," "cerebral ischemia/reperfusion," "mild cognitive impairment," "Alzheimer's disease," "vascular dementia," "vascular cognitive impairment with no dementia," "multi-infarct dementia," "synaptic injury," "functional MRI," "glucose metabolism," "NMDA," "nuclear factor-kappa b pathway," "neurotrophic factor," "matrix metalloproteinase-2 (MMP-2)," "tumor necrosis factor-alpha," "Bax," "Caspase-3," and "Noxa". Trending keywords may indicate frontier topics, such as "randomized controlled trial," "rat model," and "meta-analysis." Conclusion: This research provides valuable information for the study of acupuncture. Diseases focus on mild cognitive impairment (MCI), Alzheimer's disease (AD), and vascular dementia (VaD). Tauopathies with hyperphosphorylation of Tau protein as the main lesions also need to be paid attention to. The development of functional magnetic resonance imaging (fMRI) will better explain the therapeutic effect of acupuncture treatment. The effect of acupuncture on a single point is more convincing, and acupuncture on Baihui (GV20) may be needed in the future. Finally, the implementation of high-quality multicenter randomized controlled trials (RCTs) requires increased collaboration among experts from multiple fields and countries.

Risk assessment and hotspots identification of heavy metals in rice: A case study in Longyan of Fujian province, China.

The potential threats of heavy metals in rice have attracted increasing attention worldwide. In this study, we assessed the pollution status and health risk of rice collected from Longyan in Fujian, China. Meanwhile, we explored the spatial pattern and hotspots of those metals. The results showed that the average concentrations of Cd, Hg, As, Pb, Cr, Ni, Cu, and Zn in rice were 0.064, 0.002, 0.464, 0.072, 0.138, 0.106, 10.819, and 23.788 mg kg-1, respectively. Among them, As and Cu remarkably accumulated with the exceeding ratio of 50.30% and 55.12%, respectively. Furthermore, the values of the target hazard quotient in rice ranked as As > Cu > Zn > Cd > Pd > Ni > Hg > Cr, which As and Cu was greater than 1.0. And the carcinogenic risk values were in the order of As > Cd > Ni > Cr, which all exceeded the tolerance level (1 × 10-4). Risk assessment indicated that both children and adults were posed the non-carcinogenic and carcinogenic risk from rice intake, and As had the largest contribution rate for them. Comparison found that the spatial patterns of heavy metals distribution were consistent with the hotspots. The hotspots for As and Zn located in the western part (Changting and Wuping), Cd and Cu in the eastern part (Xinluo and Yongding), Cr and Ni were simultaneously found in the northeast (Zhangping), while Hg and Pb were mainly located in the central region (Shanghang). Overall, combining the pollution status, risk assessment, and hotspot distribution in rice, the western region (Changting and Wuping) were identified as priority areas for remediation.

Responses of glutathione and phytochelatins biosysthesis in a cadmium accumulator of Perilla frutescens (L.) Britt. under cadmium contaminated conditions.

Screening new accumulators of heavy metal and identifying their tolerance, enrichment capacity of heavy metals are currently hot issues in phytoremediation research. A series of hydroponic experiments were conducted to analyze the effects of glutathione and phytochelatins in roots, stems, and leaves of Perilla frutescens under cadmium stress. The results showed that the non-protein thiols in roots and stems mainly existed in the form of GSH, PC2, PC3, and PC4 under Cd stress condition, while in leaves they existed in the form of GSH, PC2, and PC3. Furthermore, the contents of GSH and PCs positively correlated with Cd, but negatively correlated with root vigor and chlorophyll content under Cd stress conditions. After 21 days of treatments, the contents of Cd in different parts of the plant were 1465.2-3092.9 mg· kg-1 in the roots, 199.6-478.4 mg·kg-1 in the stems and 61.3-96.9 mg· kg-1 in the leaves at 2, 5, 10 mg·L-1 Cd levels respectively, and the amount of Cd uptakes were up to 3547.7-5701.7 µg·plant-1. Therefore, P. frutescens performed high capacity in Cd accumulation, and PCs played a key role in Cd tolerance. The application prospect of the plant in phytoremediation Cd polluted soil was also discussed.

Hydrotubation combined with chinese herbal medicine for salpingitic infecundity: a systematic review and meta-analysis.

Salpingitis is a major cause of infertility. Clinical studies about hydrotubation combined with Chinese herbal medicine (CHM) for salpingitic infecundity are increasing, while systematic reviews about its efficacy remain inexistent. Assessing the effectiveness of hydrotubation combined with CHM for salpingitic infecundity. Randomized controlled trials were retrieved from different seven databases. One thousand three hundred and thirty-seven papers were collected and only 16 randomized clinical trials met the requirements and were included. The meta-analysis indicated that hydrotubation combined with CHM was associated with a higher pregnancy rate and a lower ectopic pregnancy rate compared to hydrotubation alone. The success rate of recanalization was significantly increased, as well as signs and symptoms were better alleviated in patients treated with hydrotubation combined with CHM. The clinical data available indicate that compared with hydrotubation alone, hydrotubation combined with CHM for salpingitic infecundity has better therapeutic effects.