Insect-inspired nanofibrous polyaniline multi-scale films for hybrid polarimetric imaging with scattered light.

We demonstrate a bio-inspired coating for novel imaging and sensing designs: the coating sorts different colors and linear polarizations. This coating, composed of conducting, nanofibrous polyaniline in an inverse opal film (PANI-IOF), is inexpensive and can feasibly be deposited over large areas on a range of flexible and non-flat substrates. With PANI IOFs, light is scattered into azimuthally polarized Debye rings. Subsequently, the diffracted speckle patterns carry compressed representations of the polarized illumination, which we reconstruct using shallow neural networks.

Fractal, diffraction-encoded space-division multiplexing for FSO with misalignment-robust, roaming transceivers.

For free space optical (FSO) communication, a small misalignment of the transceivers may result in link failure or severe performance degradation. It can be difficult to track the narrow optical beams over long distances. Here, we propose "diffractal space-division multiplexing" (DSDM), an FSO transmission system capable of supporting misaligned roaming transceivers. This system enables spatial multiplexing for enhanced data capacity with partial off-axis beam reception. We numerically simulate and analyze the performance of the DSDM system with a particular focus on the divergence angle, roaming area, kernel bit-error-rate (K-BER), and fractal order. Our simulation results achieve K-BERs of 10[Formula: see text] with [Formula: see text]-pixel fractal beams at link distances of 2.5 km when the receiver sizes are 30[Formula: see text] of the effective beam diameter.

[Analysis of genetic variant in a fetus featuring pontocerebellar hypoplasia type 6].

OBJECTIVE: To explore the genetic basis for a fetus with cerebellar dysplasia and widened lateral ventricles. METHODS: The couple have elected induced abortion after careful counseling. Skin tissue sample from the abortus and peripheral venous blood samples from both parents were collected for the extraction of genomic DNA, which was then subjected to whole exome sequencing. Candidate variant was verified by Sanger sequencing. RESULTS: Prenatal ultrasonography showed increased nuchal translucency (0.4 cm) and widened lateral ventricles. Magnetic resonance imaging revealed infratentorial brain dysplasia. By DNA sequencing, the fetus was found to carry compound heterozygous variants c.1A>G and c.1564G>A of the RARS2 gene, which were inherited from its father and mother, respectively. Among these, c.1A>G was known to be pathogenic, but the pathogenicity of c.1564G>A was unreported previously. Based on the American College of Medical Genetics and Genomics guidelines, the c.1564G>A variant of RARS2 gene was predicted to be likely pathogenic(PM2+PM3+PP3+PP4). CONCLUSION: The compound heterozygous variants c.1A>G and c.1564G>A of RARS2 gene contributed to the fetus suffering from pontocerebellar hypoplasia type 6, which expanded variant spectrum of RARS2 gene.

Applying Relatedness to Explain Learning Outcomes of STEM Maker Activities.

A growing interest has been observed among K-12 school educators to incorporate maker pedagogy into science, technology, engineering, and mathematics (STEM) education to engage students in the design and making process. Both cognitive engagement and emotional engagement of students can be promoted through satisfying the psychological need of relatedness that concerns a sense of connection and belonging. How to support relatedness would influence the effective development of students' cognitive competencies, namely creativity and critical thinking, and non-cognitive characteristics, namely interest and identity. Therefore, the present study investigated how two relatedness support strategies-real-world problems (RWP) and mentoring influence the development of student's STEM-related cognitive competencies and non-cognitive characteristics in STEM marker activities. We implemented a 7-week intervention study with three classes of Grade 9 students (aged 13-15 years) in Hong Kong (n = 95). Three intervention conditions were designed in the experiment, comprising textbook problem (TBP), RWP, and RWP with mentoring (RWPM). Our analysis showed that (i) the differences in creativity among the three groups were non-significant, (ii) the RWP and RWPM groups showed stronger critical thinking than the TBP group, and (iii) the RWPM group exhibited stronger STEM interest and identity than the other two groups. This study revealed the effectiveness of adopting RWP strategy in developing secondary students' perceived cognitive competencies (e.g., creativity and critical thinking) and the feasibility of employing a mentoring mechanism for cultivating learners' perceived non-cognitive characteristics (e.g., STEM identity and interest). Hence, we also offered practical suggestions for teachers.

[Genetic variant analysis of a pedigree affected with lymphedema-distichiasis syndrome].

OBJECTIVE: To analyze FOXC2 gene variant in a family affected with lymphodema-distichiasis syndrome (LDS). METHODS: Peripheral blood samples were collected for the extraction of DNA and protein. Whole-exome sequencing was carried out to detect variants in the proband. Suspected variant was validated by Sanger sequencing. Western blotting was used to detect changes in protein expression. RESULTS: The proband and his mother were both found to carry a heterozygous nonsense variant c.177C>G (p.Tyr59X) of the FOXC2 gene, which was previously unreported. Down-regulated expression of FOXC2 was detected by Western blotting. Prenatal ultrasonography of the fetus indicated increased nuchal thickness. Amniocentesis was performed at 21+1 weeks of pregnancy, genetic testing suggested that the fetus also carried the c.177C>G variant. CONCLUSION: The patients' condition may be attributed to the heterozygous nonsense variant c.177C>G of the FOXC2 gene, which resulted in a significant decrease in FOXC2 expression. Increased nuchal thickness may also be related with decreased FOXC2 expression. Above finding has expanded the variant spectrum of the FOXC2 gene.

[Identification of a Tp63 gene variant in an abortus with Ectrodactyly, Ectodermal dysplasia, Cleft lip/palate syndrome by whole-exome sequencing].

OBJECTIVE: To detect potential variant in a male fetus suspected for Ectrodactyly, Ectodermal dysplasia, Cleft lip/palate (EEC) syndrome. METHODS: Peripheral blood samples of the fetus and his parents were collected for the extraction of DNA. Whole-exome sequencing was carried out to detect potential variants. Suspected variants were verified by Sanger sequencing. RESULTS: The fetus was found to carry a heterozygous c.673C>T missense variant of the Tp63 gene, which was known to underlie split-hand/split-foot malformation. The same variant was not found in either parents. CONCLUSION: The heterozygous c.673C>T missense variant of the Tp63 gene probably underlies the EEC syndrome in the fetus. Above finding also expanded the phenotypic spectrum for this variant.

[Effect of nebulized TFG on Th1/Th2 imbalance in mouse model with asthma].

OBJECTIVE: To investigate the effect of nebulized total ginkgo flavone glycosides (TFG) on Th1/Th2 imbalance in mice with athma. METHOD: Forty-eight BALB/C mice were randomly divided into four groups: group A (control group, n=12); group B (asthmatic model group, n=12); group C (TFG nebulized treated group, n=12); group D (dexamethasone intraperitoneal treated group, n=12). The asthmatic model was established by sensitivity and local activation with Ovalbumin(OVA) and aluminum hydroxide Al(OH)3. TFG (50 g x L(-1), per aerosol per six mice, 30 minutes) was nebulized 20 days after modeling, while dexamethasone (1 g x L(-1)) was intraperitoneal once daily for 10 days. Perfusate of bronchoalveolar lavage fluid(BALF) was collected on day 32. The level of IL-4, IFN-gamma in BALF, and the level of total IgE in serum was determined. The airway inflammation pathology change and the expression of GATA-3 protein in lungs was detected by immunohistochemical staining. RESULT: Compared with model group, the decreased content of IL-4(49.30 +/- 7.95) ng x L(-1) and increased level of IFN-gamma (49.08 x 5.46) ng x L(-1). were found in BALF, and the level of total IgE (9.47 +/- 1.52) microg x L(-1) in serum also decreased in TFG treated group. In model group, smooth muscle hypertrophing, mucous hyperemia, mucous layer thickening, and inflammatory cell in filtration were observed. Phlegmasia was appeared in the bronchi, which was filled with lots of mucus. In contrast, the inflammatory reaction in TFG and Dexamethasone treated group was less obvious. Expression of GATA-3 was markly increased in model group with decreased expression in TFG treated group. CONCLUSION: Nebulized TFG showed a therapeutic effect for asthmatic mice, the mechanism may be explained by blockingnnnn GATA-3 expression and regulating the disorder Th1/Th2 imbalance.

[Effect of total flavonoid in leaves of Ginkgo biloba on the apoptosis of eosinophil in broncho alveloar lavage fluid].

This study was to investigate the effect of total flavonoid in leaves of Ginkgo biloba (total flavonoid in leaves of Ginkgo biloba, FG) on the apoptosis of eosinophils (EOS) in broncho alveloar lavage fluid (BALF) of asthma mice. Mouse asthma model was established by ovalbumin (OVA) challenge methods. After atomizing therapy for two weeks, differential count in BALF, morphological change and proportion of apoptosis were detected by AO/EB stain and Annexin V-FITC/PI. The number of total leucocytes and eosinophils in BALF decreased obviously after FG treatment. Compared with model group, the number and proportion of EOS apoptosis increased significantly after FG treatment. The results indicated that one of the anti-inflammation mechanisms of FG might be promoting apoptosis of eosinophils.