[Smoke-free Germany 2040: a discussion paper]. / Rauchfreies Deutschland 2040: Ein Diskussionsbeitrag.

INTRODUCTION: A "smoke-free" society is assumed if less than 5 % of a population smoke. We predict when this goal could be reached in Germany. To reduce the proportion of smokers in the population, the World Health Organization recommends a bundle of measures (MPOWER) that should minimize both the supply of and the demand for tobacco. The current level of implementation of these recommendations in Germany is presented. METHODS: A total of 21 representative cross-sectional surveys of the Drug Affinity Study and the Epidemiological Survey of Substance Abuse since 2000/2001 with adolescents and adults show the smoking behavior of the population until 2018. Per capita consumption of factory-built and self-made cigarettes during the same period is used as an objective data basis. Regression analyses are used to model the date at which less than 5 % of the German population smoke. A selective literature review is carried out to describe the implementation of the MPOWER program. RESULTS: Before 2000 there was no trend in Germany towards non-smoking. After the implementation of various preventive measures such as price increases for tobacco products and the introduction of non-smoking protection laws, the spread of smoking among the population has steadily decreased since 2000. By 2018, the 5 % prevalence target among adolescents had almost been reached, as the relative proportion of adolescents who smoked fell by 20.9 percentage points to 6.6 %. The relative proportion of smoking in adult women fell by 12.1 percentage points to 18.5 %, the relative proportion of smoking in men by 14.8 percentage points to 24.2 %. Assuming a linear trend, the prevalence target of less than 5 % smoking adults can be reached by around 2043. Of the six recommended measures of the MPOWER program, Germany is currently only implementing the monitoring of tobacco consumption in society without compromises. DISCUSSION: After various tobacco prevention measures were implemented, a trend towards non-smoking began in Germany. The continual price increase recommended by the World Health Organization, the further restriction of availability, the ban on all tobacco advertising and promotion, the support of smoking cessation as well as the education of the population appear to be necessary to reinforce this trend and to achieve the health policy goal of a smoke-free society in 2040.

[Enzymes of the Xenobiotic Metabolism of Continuous Cell Lines in invitro Toxicity Testing]

Cells in continuous culture contain a large number of enzymes which are involved in the metabolism of potentially toxic chemicals. As a rule, the activities of these enzymes represent functions of low tussle specificity. In contrast, those functions which are specialized "differentiated" functions in vivo are no longer expressed in continuous cell lines. However, an increasing number of observations indicates that cell lines may also contain these functions. Cell lines which lack or possess specific xenobiotic metabolizing enzymes are already applicable for analyzing the complex mechanisms of activation and inactivation of chemicals. With a better understanding how differentiated cell functions are regulated, prospects are promising for establishing metabolically competent cell lines, which can also be used in the screening of toxic chemicals.