RESUMO
Metabolic dysfunction-associated steatotic liver disease (MASLD) occurs in subjects with obesity and metabolic syndrome. MASLD may progress from simple steatosis (i.e., hepatic steatosis) to steatohepatitis, characterized by inflammatory changes and liver cell damage, substantially increasing mortality. Lifestyle measures associated with weight loss and/or appropriate diet help reduce liver fat accumulation, thereby potentially limiting progression to steatohepatitis. As for diet, both total energy and macronutrient composition significantly influence the liver's fat content. For example, the type of dietary fatty acids can affect the metabolism of lipids and hence their tissue accumulation, with saturated fatty acids having a greater ability to promote fat storage in the liver than polyunsaturated ones. In particular, polyunsaturated fatty acids of n-3 series (omega-3), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), have been intensively studied for their antisteatotic effects, both in preclinical animal models of obesity and hepatic steatosis and in overweight/obese patients. Their effects may depend not only on the dose and duration of administration of omega-3, or DHA/EPA ratio, but also on the lipid class used for their supplementation. This review summarizes the available evidence from recent comparative studies using omega-3 supplementation via different lipid classes. Albeit the evidence is mainly limited to preclinical studies, it suggests that phospholipids and possibly wax esters could provide greater efficacy against MASLD compared to traditional chemical forms of omega-3 supplementation (i.e., triacylglycerols, ethyl esters). This cannot be attributed solely to improved EPA and/or DHA bioavailability, but other mechanisms may be involved. Keywords: MASLD ⢠Metabolic dysfunction-associated steatotic liver disease ⢠NAFLD ⢠Non-alcoholic fatty liver disease ⢠n-3 polyunsaturated fatty acids.
Assuntos
Suplementos Nutricionais , Ácidos Graxos Ômega-3 , Fígado , Humanos , Animais , Ácidos Graxos Ômega-3/administração & dosagem , Ácidos Graxos Ômega-3/metabolismo , Ácidos Graxos Ômega-3/uso terapêutico , Fígado/metabolismo , Fígado/efeitos dos fármacos , Fígado/patologia , Fígado Gorduroso/metabolismo , Fígado Gorduroso/tratamento farmacológico , Metabolismo dos Lipídeos/efeitos dos fármacos , Hepatopatia Gordurosa não Alcoólica/metabolismo , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/dietoterapia , Hepatopatia Gordurosa não Alcoólica/patologia , Obesidade/metabolismo , Obesidade/tratamento farmacológico , Obesidade/dietoterapia , Obesidade/patologiaRESUMO
Mitochondria, the organelles that function as the powerhouse of the cell, have been increasingly linked to the pathogenesis of many neurological disorders, including multiple sclerosis (MS). MS is a chronic inflammatory demyelinating disease of the central nervous system (CNS) and a leading cause of neurological disability in young adults in the western world. Its etiology remains unknown, and while the inflammatory component of MS has been heavily investigated and targeted for therapeutic intervention, the failure of remyelination and the process of axonal degeneration are still poorly understood. Recent studies suggest a role of mitochondrial dysfunction in the neurodegenerative aspects of MS. This review is focused on mitochondrial functions under physiological conditions and the consequences of mitochondrial alterations in various CNS disorders. Moreover, we summarize recent findings linking mitochondrial dysfunction to MS and discuss novel therapeutic strategies targeting mitochondria-related pathways as well as emerging experimental approaches for modeling mitochondrial disease.
Assuntos
Mitocôndrias/metabolismo , Esclerose Múltipla/genética , Animais , Modelos Animais de Doenças , Humanos , Modelos Biológicos , Terapia de Alvo Molecular , Esclerose Múltipla/patologia , Esclerose Múltipla/terapia , Degeneração Neural/patologiaRESUMO
The term mitochondrial permeability transition (MPT) is commonly used to indicate an abrupt increase in the permeability of the inner mitochondrial membrane to low molecular weight solutes. Widespread MPT has catastrophic consequences for the cell, de facto marking the boundary between cellular life and death. MPT results indeed in the structural and functional collapse of mitochondria, an event that commits cells to suicide via regulated necrosis or apoptosis. MPT has a central role in the etiology of both acute and chronic diseases characterized by the loss of post-mitotic cells. Moreover, cancer cells are often relatively insensitive to the induction of MPT, underlying their increased resistance to potentially lethal cues. Thus, intense efforts have been dedicated not only at the understanding of MPT in mechanistic terms, but also at the development of pharmacological MPT modulators. In this setting, multiple mitochondrial and extramitochondrial proteins have been suspected to critically regulate the MPT. So far, however, only peptidylprolyl isomerase F (best known as cyclophilin D) appears to constitute a key component of the so-called permeability transition pore complex (PTPC), the supramolecular entity that is believed to mediate MPT. Here, after reviewing the structural and functional features of the PTPC, we summarize recent findings suggesting that another of its core components is represented by the c subunit of mitochondrial ATP synthase.
Assuntos
Morte Celular , Mitocôndrias/metabolismo , Membranas Mitocondriais/metabolismo , ATPases Mitocondriais Próton-Translocadoras/metabolismo , Animais , Permeabilidade da Membrana Celular , Peptidil-Prolil Isomerase F , Ciclofilinas/metabolismo , Humanos , Proteínas de Transporte da Membrana Mitocondrial/metabolismo , ATPases Mitocondriais Próton-Translocadoras/químicaRESUMO
Mitochondrial defects, affecting parameters such as mitochondrial number and shape, levels of respiratory chain complex components and markers of oxidative stress, have been associated with the appearance and progression of multiple sclerosis. Nevertheless, mitochondrial physiology has never been monitored during oligodendrocyte progenitor cell (OPC) differentiation, especially in OPCs challenged with proinflammatory cytokines. Here, we show that tumor necrosis factor alpha (TNF-α) inhibits OPC differentiation, accompanied by altered mitochondrial calcium uptake, mitochondrial membrane potential, and respiratory complex I activity as well as increased reactive oxygen species production. Treatment with a mitochondrial uncoupler (FCCP) to mimic mitochondrial impairment also causes cells to accumulate at the progenitor stage. Interestingly, AMP-activated protein kinase (AMPK) levels increase during TNF-α exposure and inhibit OPC differentiation. Overall, our data indicate that TNF-α induces metabolic changes, driven by mitochondrial impairment and AMPK activation, leading to the inhibition of OPC differentiation.
Assuntos
Mitocôndrias/metabolismo , Oligodendroglia/citologia , Oligodendroglia/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Animais , Diferenciação Celular/fisiologia , Esclerose Múltipla/metabolismo , Esclerose Múltipla/patologia , Ratos , Espécies Reativas de Oxigênio/metabolismo , Transdução de SinaisRESUMO
This study reports clinical, biochemical and histopathological findings associated with a novel homozygous MPV17 mutation in four patients with mitochondrial depletion syndrome. The severe course of the disease, which started in the first weeks of life, was dominated by a failure to thrive, hypotonia and liver dysfunction, with relatively mild neurological involvement. All affected infants died by 1 year of age. Laboratory findings included progressive liver failure (hypertransaminasaemia, icterus, and coagulopathy), recurrent hypoglycaemia, lactic acidaemia, hyperferritinaemia, and increased transferrin saturation. Histological and ultrastructural analyses uncovered significant lipid accumulation in hepatocytes and myocytes. A severe decrease in the mitochondrial/nuclear DNA (mtDNA/nDNA) ratio was found post-mortem in the livers (and in one muscle specimen) of both examined patients. Oxidative phosphorylation system (OXPHOS) Western blotting revealed low levels of complexes I, III and IV subunits. The highlights of our findings are as follows: (i) The novel p.Pro64Arg mutation is the second recurrent MPV17 mutation reported. The phenotype associated with the p.Pro64Arg mutation differs from the phenotype of the relatively common p.Arg50Gln mutation, suggesting the existence of a genotype-phenotype correlation. (ii) Tissues collected from patients during autopsy may be useful for both mtDNA/nDNA ratio assessment and OXPHOS Western blotting.
Assuntos
Encefalopatia Hepática/genética , Proteínas de Membrana/genética , Mitocôndrias/genética , Doenças Mitocondriais/genética , Proteínas Mitocondriais/genética , Mutação , Sequência de Bases , Evolução Fatal , Feminino , Encefalopatia Hepática/congênito , Encefalopatia Hepática/metabolismo , Encefalopatia Hepática/patologia , Humanos , Lactente , Masculino , Proteínas de Membrana/metabolismo , Mitocôndrias/metabolismo , Mitocôndrias/patologia , Doenças Mitocondriais/congênito , Doenças Mitocondriais/metabolismo , Doenças Mitocondriais/patologia , Proteínas Mitocondriais/metabolismo , Dados de Sequência Molecular , Fosforilação Oxidativa , Polônia , IrmãosRESUMO
Primary mitochondrial dysfunction commonly leads to failure in cellular adaptation to stress. Paradoxically, however, nonsynonymous mutations of mitochondrial DNA (mtDNA) are frequently found in cancer cells and may have a causal role in the development of resistance to genotoxic stress induced by common chemotherapeutic agents, such as cis-diammine-dichloroplatinum(II) (cisplatin, CDDP). Little is known about how these mutations arise and the associated mechanisms leading to chemoresistance. Here, we show that the development of adaptive chemoresistance in the A549 non-small-cell lung cancer cell line to CDDP is associated with the hetero- to homoplasmic shift of a nonsynonymous mutation in MT-ND2, encoding the mitochondrial Complex-I subunit ND2. The mutation resulted in a 50% reduction of the NADH:ubiquinone oxidoreductase activity of the complex, which was compensated by increased biogenesis of respiratory chain complexes. The compensatory mitochondrial biogenesis was most likely mediated by the nuclear co-activators peroxisome proliferator-activated receptor gamma co-activator-1α (PGC-1α) and PGC-1ß, both of which were significantly upregulated in the CDDP-resistant cells. Importantly, both transient and stable silencing of PGC-1ß re-established the sensitivity of these cells to CDDP-induced apoptosis. Remarkably, the PGC-1ß-mediated CDDP resistance was independent of the mitochondrial effects of the co-activator. Altogether, our results suggest that partial respiratory chain defects because of mtDNA mutations can lead to compensatory upregulation of nuclear transcriptional co-regulators, in turn mediating resistance to genotoxic stress.
Assuntos
Proteínas de Transporte/metabolismo , DNA Mitocondrial , Resistencia a Medicamentos Antineoplásicos/genética , Mutação , Adaptação Fisiológica , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Proteínas de Transporte/genética , Linhagem Celular Tumoral , Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , NADH Desidrogenase/genética , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo , Proteínas de Ligação a RNA , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Mammalian life span can be controlled by p66Shc protein through regulation of cellular response to oxidative stress. We investigated age-related changes in the amount of p66Shc and its Ser36-phosphorylated form in various mouse organs and tissues and correlated it with the level of antioxidant enzymes. Comparing to the newborn, in adult 6-month-old mice, the level of p66Shc was increased particularly in liver, lungs, skin and diaphragm. In older animals the level of p66Shc decreased while signaling pathway responsible for Ser36 phosphorylation of p66Shc protein seemed to be continually enhanced. The amount of p66Shc phosphorylated at Ser36, significantly increased with age, resulted in higher free radical production and, in consequence accumulation of damages caused by free radicals. The increased amount of Ser36-phosphorylated p66Shc in livers of 12- and 23-month-old mice was correlated with the decreased level of antioxidant enzymes. Moreover, we found that p66Shc is a resident of mitochondria- and plasma membrane-associated membranes and that its level there depends on the age of animal.
Assuntos
Envelhecimento/metabolismo , Proteínas Adaptadoras da Sinalização Shc/metabolismo , Animais , Animais Recém-Nascidos , Antioxidantes/metabolismo , Células Cultivadas , Feminino , Radicais Livres/metabolismo , Fígado/metabolismo , Camundongos , Modelos Biológicos , Fosforilação , Serina/química , Proteínas Adaptadoras da Sinalização Shc/química , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src , Frações Subcelulares/metabolismo , Distribuição TecidualRESUMO
Recent data shed light on two novel aspects of the mitochondria-Ca2+ liaison. First, it was extensively investigated how Ca2+ handling is controlled by mitochondrial shape, and positioning; a playground also of cell death and survival regulation. On the other hand, significant progress has been made to explore how intra- and near-mitochondrial Ca2+ signals modify mitochondrial morphology and cellular distribution. Here, we shortly summarize these advances and provide a model of Ca2+-mitochondria interactions.
Assuntos
Sinalização do Cálcio , Mitocôndrias/metabolismo , Animais , Evolução Biológica , Retículo Endoplasmático/metabolismo , HumanosAssuntos
Trifosfato de Adenosina/biossíntese , Proteínas de Ligação ao GTP/metabolismo , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Miócitos Cardíacos/metabolismo , Transglutaminases/metabolismo , Animais , Proteínas de Ligação ao GTP/deficiência , Proteínas de Ligação ao GTP/genética , Técnicas In Vitro , Masculino , Potenciais da Membrana , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Mitocôndrias Cardíacas/metabolismo , Traumatismo por Reperfusão Miocárdica/metabolismo , Miócitos Cardíacos/citologia , Proteína 2 Glutamina gama-Glutamiltransferase , Receptores Adrenérgicos alfa 1/deficiência , Receptores Adrenérgicos alfa 1/genética , Receptores Adrenérgicos alfa 1/metabolismo , Transdução de Sinais , Transglutaminases/deficiência , Transglutaminases/genéticaRESUMO
UNLABELLED: The mechanism by which the proapoptotic protein Bax releases cytochrome c from mitochondria is not fully understood. The present work approaches this problem using C-terminal truncated oligomeric Bax (BaxDeltaC). Micromolar concentrations of BaxDeltaC released cytochrome c from isolated rat heart and liver mitochondria, while the release of adenylate kinase was not significantly affected. BaxDeltaC also released cytochrome c but not adenylate kinase from outer membrane vesicles filled with these proteins. However, BaxDeltaC was ineffective in releasing cytochrome c when outer membrane vesicles were obtained in the presence of glycerol, conditions under which the number of contact sites was drastically reduced. BaxDeltaC did not liberate encapsulated cytochrome c and adenylate kinase from pure phospholipid vesicles or vesicles reconstituted with porin. However, when the hexokinase-porin-adenine nucleotide translocase complex from brain mitochondria was reconstituted in vesicles, BaxDeltaC released internal cytochrome c but not adenylate kinase. In all these systems, only a small portion of total cytochrome c present in either mitochondria or vesicles could be liberated by BaxDeltaC. BaxDeltaC also increased the accessibility of external cytochrome c to either oxidation by complex IV or reduction by complex III in intact liver and heart mitochondria. CONCLUSIONS: (1) BaxDeltaC selectively releases cytochrome c and enables a bidirectional movement of cytochrome c across the outer mitochondrial membrane. (2) A multiprotein complex that resembles the mitochondrial contact sites is a prerequisite for BaxDeltaC action. (3) A limited pool of cytochrome c becomes the first target for BaxDeltaC.
Assuntos
Adenilato Quinase/metabolismo , Grupo dos Citocromos c/metabolismo , Mitocôndrias Cardíacas/enzimologia , Mitocôndrias Hepáticas/enzimologia , Proteolipídeos , Proteínas Proto-Oncogênicas c-bcl-2 , Proteínas Proto-Oncogênicas/metabolismo , Animais , Biopolímeros , Membrana Celular/enzimologia , Masculino , Complexos Multiproteicos , Proteínas Proto-Oncogênicas/química , Ratos , Ratos Wistar , Proteína X Associada a bcl-2RESUMO
Adenine nucleotide translocase-porin-hexokinase complex isolated from rat brain, when reconstituted into phospholipid-cholesterol vesicles, exhibits all properties of the mitochondrial permeability transition pore [Beutner, G., Rück, A., Riede, B., Welte, W. and Brdiczka, D. (1996) FEBS Lett. 396, 189-195]. In the present work, the effect of long-chain fatty acids on such reconstituted pore was examined. Opening of the pore was measured by leakage of either malate or fluorescein sulphonate entrapped inside the vesicles. It was found that myristate and oleate in the presence of 50 or 100 microM Ca(2+) produced a partial release of the probes in a dose-dependent way. A dicarboxylic fatty acid analogue, that appeared inactive as protonophore in intact mitochondria, exerted no effect on pore opening in the reconstituted system. 100 microM Ca(2+) alone was without effect. Pore opening by fatty acids in the reconstituted system was partly prevented by cyclosporin A. The pore opening also occurred when the vesicles were incubated in the presence of pancreatic phospholipase A(2). In this case, the opening was decreased by cyclosporin A or serum albumin. These results indicate that long-chain fatty acids elicit opening of the permeability transition pore reconstituted in phospholipid vesicles in a similar way as in intact mitochondria [Wi&ecedil;ckowski, M.R. and Wojtczak, L. (1998) FEBS Lett. 423, 339-342].
Assuntos
Ácidos Graxos/fisiologia , Mitocôndrias/fisiologia , Animais , Técnicas In Vitro , Substâncias Macromoleculares , Membranas Artificiais , Permeabilidade , Fosfolipídeos/fisiologia , RatosRESUMO
Swelling of non-respiring rat liver mitochondria suspended in isotonic potassium acetate at pH 6.5-7.4 in the presence of valinomycin was promoted by long-chain fatty acids, such as myristate, indicating a protonophoric mechanism. This swelling was partly inhibited by inhibitors or substrates of mitochondrial anion carriers. The results show that the fatty acid cycling mechanism responsible for uncoupling of oxidative phosphorylation can also operate in the direction opposite to that originally proposed [Skulachev, V.P. (1991) FEBS Lett. 294, 158-162], i.e. the inwardly directed transfer of the fatty acid anion accompanied by outwardly directed free passage of undissociated fatty acid. They also extend the list of mitochondrial anion carriers, that are involved in this process, over the mono- and tricarboxylate transporters. At pH 8, myristate, but not the synthetic protonophore, p-trifluoromethoxycarbonyl-cyanide phenylhydrazone, induced mitochondrial swelling in both potassium acetate and KCl media, that did not require the presence of valinomycin. This indicates that, at alkaline pH, myristate facilitates permeation of the inner mitochondrial membrane to monovalent cations and, possibly, activates the inner membrane anion channel.
Assuntos
Ácidos Graxos/metabolismo , Mitocôndrias Hepáticas/metabolismo , Dilatação Mitocondrial/fisiologia , Animais , Ácidos Graxos/fisiologia , Feminino , Técnicas In Vitro , Membranas Intracelulares/metabolismo , Ratos , Ratos WistarRESUMO
The distribution of Ca(2+) in intact cells was monitored with fluorescent probes: fura-2 for cytosolic [Ca(2+)] and rhod-2 for mitochondrial [Ca(2+)]. It was found that in neoplastic cells, such as Ehrlich ascites tumour and Zajdela hepatoma, but not in non-malignant cells, such as fibroblasts, glucose and deoxyglucose elicited release of Ca(2+) from endoplasmic reticulum stores and an increase in Ca(2+) concentration in the cytosol. Parallel to this, a decrease in the rate of Ca(2+) extrusion from the cell and an enhanced uptake of Ca(2+) by mitochondria were observed. The increase in mitochondrial [Ca(2+)] was accompanied by an increase in the mitochondrial membrane potential and the reduction state of nicotinamide nucleotides. F(1)F(o)-ATPase in submitochondrial particles of Zajdela hepatoma was strongly inhibited in the presence of micromolar Ca(2+) concentrations, whereas this activity in submitochondrial particles from rat liver appeared to be less sensitive to Ca(2+). Indications of glycosylation of Ehrlich ascites tumour cell proteins were also obtained. These data strengthen the proposal [Bogucka, K., Teplova, V.V., Wojtczak, L. and Evtodienko, Y. V. (1995) Biochim. Biophys. Acta 1228, 261-266] that the Crabtree effect is produced by mobilization of cell calcium, which is subsequently taken up by mitochondria and inhibits F(1)F(o)-ATP synthase.
Assuntos
Cálcio/fisiologia , Carcinoma de Ehrlich/metabolismo , Desoxiglucose/farmacologia , Glucose/farmacologia , Neoplasias Hepáticas Experimentais/metabolismo , Mitocôndrias/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Cálcio/farmacologia , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Fibroblastos , Humanos , Cinética , Fígado/metabolismo , Ratos , Ratos Wistar , Espectrofotometria , Tapsigargina/farmacologia , Fatores de Tempo , Células Tumorais CultivadasRESUMO
We have shown previously [E. Sikora, A. Bielak-Zmijewska, K. Piwocka, J. Skierski, and E. Radziszewska (1997) Biochem. Pharmacol. 54, 899-907] that curcumin prevents formation of oligonucleosomal DNA fragmentation in rat thymocytes and human leukemic T lymphocytes (Jurkat cells) induced to undergo apoptosis. In this paper we show that 50 microM curcumin by itself induces cell death in Jurkat cells, but its symptoms differ from those observed after a short ultraviolet (uv) irradiation. Ultraviolet-irradiated Jurkat cells displayed typical symptoms of apoptosis: morphological changes, internucleosomal and high-molecular-weight DNA fragmentation, formation of sub-G1 fractions in DNA content frequency histograms, and dissipation of the mitochondrial transmembrane electric potential (Delta psi). In contrast, curcumin-treated Jurkat cells exhibited DNA splitting into high-, but not low-, molecular-weight fragments. These cells retained their high mitochondrial Delta psi, and the content of Ca2+ in endoplasmic reticulum stores remained at the level typical for untreated cells. The frequency of opening of the mitochondrial permeability transition pores in curcumin-treated cells was decreased compared to the controls, whereas uv irradiation made these pores completely open. Curcumin did not produce any change in the activity of caspase-3, whereas uv irradiation considerably activated this protease. The morphology of curcumin-treated cells displayed chromatin condensation, which was insensitive to the caspase inhibitor z-VAD-fmk, but no formation of typical apoptotic bodies, as was the case after uv irradiation. In contrast to uv-irradiated cells, curcumin-treated Jurkat cells considerably increased the level of Bcl-2. It is concluded that the programmed cell death induced by curcumin in Jurkat cells differs from "classical" by the lack of mitochondrial depolarization and of the involvement of caspases.
Assuntos
Apoptose/efeitos dos fármacos , Caspases/metabolismo , Curcumina/farmacologia , Mitocôndrias/efeitos dos fármacos , Linfócitos T/efeitos dos fármacos , Linfócitos T/enzimologia , Antineoplásicos/farmacologia , Cálcio/metabolismo , Caspase 3 , Linhagem Celular Transformada , Fragmentação do DNA/efeitos dos fármacos , Humanos , Células Jurkat , Mitocôndrias/enzimologia , Permeabilidade , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Linfócitos T/citologiaRESUMO
Nonesterified long-chain fatty acids have long been known as uncouplers of oxidative phosphorylation. They are efficient protonophores in the inner mitochondrial membrane but not so in artificial phospholipid membranes. In the un-ionized form, they undergo a rapid spontaneous transbilayer movement (flip-flop). However, the transbilayer passage of the dissociated (anionic) form is hindered by the negatively charged hydrophilic carboxylic group. In the inner mitochondrial membrane, the transfer of fatty acid anions is mediated by the adenine nucleotide translocase, the dicarboxylate carrier, and the glutamate/aspartate carrier. As a result, the passage of protons and electric charges is a concerted effect of the spontaneous flip-flop of the undissociated (protonated) form in one direction and carrier-facilitated transfer of the ionized (deprotonated) form in the other direction. In addition, fatty acids also promote opening of the mitochondrial permeability transition pore, presumably due to their interaction with one of its constituents, the adenine nucleotide translocase, thus forming an additional route for dissipation of the proton gradient. Structural prerequisites for these proton-conducting mechanisms are (1) a weakly ionized carboxylic group and (2) a hydrocarbon chain of appropriate length without substituents limiting its mobility and hydrophobicity.
Assuntos
Ácidos Graxos/metabolismo , Membranas Intracelulares/metabolismo , Mitocôndrias/metabolismo , Prótons , Animais , Cátions Monovalentes , Permeabilidade da Membrana Celular , Ionóforos , Metais , Translocases Mitocondriais de ADP e ATP/metabolismo , PermeabilidadeRESUMO
The protonophoric (uncoupling) action of various long-chain fatty acids and their derivatives in mitochondria was investigated as related to their ability for rapid transbilayer movement in the inner mitochondrial membrane (flip-flop) and interaction with the ADP/ATP carrier (AAC). Flip-flop was assessed from a rapid decrease of internal mitochondrial pH. It was found that long-chain unsubstituted fatty acids (with the exception of very-long-chain unbranched homologs) and their thia and oxa analogs performed a rapid flip-flop, inhibited AAC activity and increased proton permeability of the inner mitochondrial membrane, resulting in dissipation of mitochondrial membrane potential and increased resting state respiration. Bipolar fatty acid analogs, i.e., those containing a second carboxylic group or OH group(s) at the hydrocarbon tail, phenyl-substituted fatty acid derivatives, and fatty acid analogs containing strongly ionized sulfonyl or sulfate groups instead of the carboxylic group, did not flip-flop and were not uncoupling, although some of them were weak inhibitors of AAC. These results provide further confirmation of the fatty acid cycling model (V. P. Skulachev, FEBS Lett. 294, 158-162, 1991) in which the protonophoric function of fatty acids is a result of the spontaneous transbilayer passage of undissociated (protonated) molecules of the fatty acid from the external side of the inner mitochondrial membrane to the matrix side and the AAC-mediated transport of the fatty acid anion in the opposite direction.
Assuntos
Ácidos Graxos/metabolismo , Membranas Intracelulares/metabolismo , Mitocôndrias Hepáticas/metabolismo , Prótons , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Transporte Biológico , Respiração Celular , Masculino , Potenciais da Membrana , Mitocôndrias Hepáticas/química , Translocases Mitocondriais de ADP e ATP/metabolismo , Dilatação Mitocondrial , Ratos , Ratos WistarRESUMO
Addition of myristate at low concentration (30-60 nmol/mg protein) to energized rat liver mitochondria resulted in dissipation of the electric membrane potential which, in Ca2+-free media, could be partly reversed by carboxyatractyloside but not by cyclosporin A. In contrast, in mitochondria preloaded with Ca2+ this energy-dissipating effect of fatty acid was partly prevented or reversed by cyclosporin A or ADP. In sucrose media, myristate, but not the protonophore carbonyl cyanide m-chlorophenylhydrazone, induced swelling of Ca2+-loaded mitochondria which was inhibited by cyclosporin A and ADP. We conclude that long-chain fatty acids may induce opening of the mitochondrial permeability transition pore not only because of their protonophoric effect mediated by mitochondrial anion carriers [Skulachev, V.P., FEBS Lett. 294 (1991) 158-162; Wieckowski, M.R. and Wojtczak, L., Biochem. Biophys. Res. Commun. (1997) 232, 414-417] but also by a direct interaction with the pore assembly.
Assuntos
Ácidos Graxos/farmacologia , Membranas Intracelulares/efeitos dos fármacos , Proteínas de Membrana/fisiologia , Mitocôndrias Hepáticas/efeitos dos fármacos , Fosforilação Oxidativa/efeitos dos fármacos , Porinas , Difosfato de Adenosina/farmacologia , Animais , Atractilosídeo/análogos & derivados , Atractilosídeo/farmacologia , Cálcio/metabolismo , Carbonil Cianeto m-Clorofenil Hidrazona/farmacologia , Ciclosporina/farmacologia , Canais Iônicos/fisiologia , Masculino , Potenciais da Membrana/efeitos dos fármacos , Dilatação Mitocondrial/efeitos dos fármacos , Ácido Mirístico/farmacologia , Permeabilidade/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Desacopladores/farmacologia , Canais de Ânion Dependentes de VoltagemRESUMO
Liver mitochondria from rats made hypothyroid by administration of 2-mercapto-1-methylimidazole were less sensitive to the uncoupling effect of myristic acid, as measured by the increase of resting state respiration, than mitochondria from euthyroid animals, whereas subsequent administration to the animals of triiodothyronine ('hyperthyroidism') resulted in an increased uncoupling action of myristate. 'Hyperthyroidism' also resulted in doubling of the carboxyatractyloside-sensitive portion of the myristate-stimulated respiration. Parallel to this was a twofold increase of the mitochondrial content of the ADP/ATP carrier protein and an over threefold increase of its activity. The uncoupling effect of phytanic acid was less sensitive to carboxyatractyloside and was increased in the hyperthyroid state to a smaller extent than in the case of myristate. These results provide further support to the thesis [Skulachev, V.P., FEBS Lett. 294 (1991) 158-162] that the ADP/ATP carrier is involved in the mechanism of the uncoupling effect of long-chain fatty acids.
Assuntos
Ácidos Graxos/farmacologia , Mitocôndrias Hepáticas/efeitos dos fármacos , Translocases Mitocondriais de ADP e ATP/metabolismo , Tri-Iodotironina/farmacologia , Animais , Feminino , Mitocôndrias Hepáticas/metabolismo , Fosforilação Oxidativa , Oxigênio/metabolismo , Ratos , Ratos WistarRESUMO
Resting state respiration of rat liver mitochondria with succinate or N,N,N',N'-tetrametnyl-p-phenylene-diamine plus ascorbate as substrates was stimulated by myristate. This uncoupling effect was partly reversed not only by carboxyatractyloside and glutamate as reported by others [V. P. Skulachev (1991) FEBS Lett. 294, 158-162; V.N. Samartsev, A.V. Smirnov, I.P. Zeldi, O.V. Markova, E.N. Mokhova, and V.P. Skulachev (1997) Biochim. Biophys. Acta, in press] but also by malonate. Glutamate and malonate also partly restored the mitochondrial membrane potential dissipated by addition of myristate. Myristate inhibited the transport of malonate through the mitochondrial inner membrane, 50% inhibition being reached with 100 nmol fatty acid/mg mitochondrial protein. A similar inhibitory effect was obtained with an azido derivative of long-chain fatty acid, 12-(4-azido-2-nitrophenylamino) dodecanoic acid. This inhibitory effect could be reversed by serum albumin. However, after illumination with ultraviolet light, the inhibition of malonate transport could not be reversed by serum albumin, pointing to photomodification of the dicarboxylate carrier. These results indicate that the dicarboxylate carrier, along with the ADP/ATP carrier and the glutamate carrier, participates in the protonophoric action of fatty acids in mitochondria.
Assuntos
Proteínas de Transporte/fisiologia , Ácidos Dicarboxílicos/química , Ácidos Graxos/farmacologia , Mitocôndrias Hepáticas/efeitos dos fármacos , Prótons , Animais , Atractilosídeo/análogos & derivados , Atractilosídeo/farmacologia , Transportadores de Ácidos Dicarboxílicos , Glutamatos/farmacologia , Masculino , Malonatos/farmacologia , NADH Desidrogenase/antagonistas & inibidores , Fosforilação Oxidativa , Consumo de Oxigênio , Ratos , Rotenona/farmacologia , DesacopladoresRESUMO
Azido derivatives of long-chain fatty acids, 12-(4-azido-2-nitrophenylamino)dodecanoic acid (N3-NpNH-Lau) and 16-(4-azido-2-nitrophenylamino)hexadecanoic acid (N3-NpNH-Pam), were used to study the mechanism of the protonophoric function of long-chain fatty acids in mitochondrial membranes. N3-NpNH-Lau was found to increase resting-state respiration and decrease the membrane potential in a dose-dependent way in a manner similar to that of the natural fatty acid, myristate. Both effects of N3-NpNH-Lau as well as of the myristate were reversed or prevented by the inhibitor of the mitochondrial ADP/ATP carrier (AAC), carboxyatractyloside. This protective effect of carboxyatractyloside was well expressed in rat heart mitochondria and less so in mitochondria within digitonin-permeabilized Ehrlich ascites tumour cells. Photomodification of Ehrlich ascites tumour mitochondria by ultraviolet irradiation in the presence of N3-NpNH-Lau made them more resistant to the uncoupling effect of myristate, and photomodification of rat heart mitochondria resulted in a strong inhibition of AAC which could not be reversed by serum albumin. Photolabelling of rat heart mitochondria with tritiated N3-NpNH-Pam revealed around 10 labelled bands on SDS/polyacrylamide gel electrophoresis. Based on immunodetection with a specific antibody, one of them, corresponding to 30 kDa, was identified as AAC. Specific interaction of AAC with azido fatty acids was confirmed by a high radiolabelling of this band. The role of fatty acids in fine control of the efficiency of oxidative phosphorylation is discussed.