Novel insights into maize (Zea mays) development and organogenesis for agricultural optimization.

MAIN CONCLUSION: In maize, intrinsic hormone activities and sap fluxes facilitate organogenesis patterning and plant holistic development; these hormone movements should be a primary focus of developmental biology and agricultural optimization strategies. Maize (Zea mays) is an important crop plant with distinctive life history characteristics and structural features. Genetic studies have extended our knowledge of maize developmental processes, genetics, and molecular ecophysiology. In this review, the classical life cycle and life history strategies of maize are analyzed to identify spatiotemporal organogenesis properties and develop a definitive understanding of maize development. The actions of genes and hormones involved in maize organogenesis and sex determination, along with potential molecular mechanisms, are investigated, with findings suggesting central roles of auxin and cytokinins in regulating maize holistic development. Furthermore, investigation of morphological and structural characteristics of maize, particularly node ubiquity and the alternate attachment pattern of lateral organs, yields a novel regulatory model suggesting that maize organ initiation and subsequent development are derived from the stimulation and interaction of auxin and cytokinin fluxes. Propositions that hormone activities and sap flow pathways control organogenesis are thoroughly explored, and initiation and development processes of distinctive maize organs are discussed. Analysis of physiological factors driving hormone and sap movement implicates cues of whole-plant activity for hormone and sap fluxes to stimulate maize inflorescence initiation and organ identity determination. The physiological origins and biogenetic mechanisms underlying maize floral sex determination occurring at the tassel and ear spikelet are thoroughly investigated. The comprehensive outline of maize development and morphogenetic physiology developed in this review will enable farmers to optimize field management and will provide a reference for de novo crop domestication and germplasm improvement using genome editing biotechnologies, promoting agricultural optimization.

Drivers of recent decline in dust activity over East Asia.

It is essential to understand the factors driving the recent decline of dust activity in East Asia for future dust projections. Using a physically-based dust emission model, here we show that the weakening of surface wind and the increasing of vegetation cover and soil moisture have all contributed to the decline in dust activity during 2001 to 2017. The relative contributions of these three factors to the dust emission reduction during 2010-2017 relative to 2001 are 46%, 30%, and 24%, respectively. Much (78%) of the dust emission reduction is from barren lands, and a small fraction (4.6%) of the reduction is attributed to grassland vegetation increase that is partly ascribed to the ecological restoration. This suggests that the ecological restoration plays a minor role in the decline of dust activity. Rather, the decline is mainly driven by climatic factors, with the weakening of surface wind playing the dominant role.

Black carbon-climate interactions regulate dust burdens over India revealed during COVID-19.

India as a hotspot for air pollution has heavy black carbon (BC) and dust (DU) loadings. BC has been identified to significantly impact the Indian climate. However, whether BC-climate interactions regulate Indian DU during the premonsoon season is unclear. Here, using long-term Reanalysis data, we show that Indian DU is positively correlated to northern Indian BC while negatively correlated to southern Indian BC. We further identify the mechanism of BC-dust-climate interactions revealed during COVID-19. BC reduction in northern India due to lockdown decreases solar heating in the atmosphere and increases surface albedo of the Tibetan Plateau (TP), inducing a descending atmospheric motion. Colder air from the TP together with warmer southern Indian air heated by biomass burning BC results in easterly wind anomalies, which reduces dust transport from the Middle East and Sahara and local dust emissions. The premonsoon aerosol-climate interactions delay the outbreak of the subsequent Indian summer monsoon.

Genome-Wide Identification of Maize Aquaporin and Functional Analysis During Seed Germination and Seedling Establishment.

Water uptake facilitates the initiation of seed germination. It is presumed that aquaporin (AQP)-mediated water inflow contributes to seed germination, but the genetic evidence is still lacking. This study aimed at genome-wide identification of ZmAQPs and further determined the physiological functions. Following a comprehensive search, a total of 41 ZmAQPs were identified according to the latest genome database. Through bioinformatic approaches, the physicochemical characteristics, phylogenetic relationships, and structural features of ZmAQPs were analyzed. The gene expression analysis of 20 high-resolution and multi-tissues samples showed that ZmAQPs had distinct spatiotemporal and tissue-specific expression profiles during seed germination and early seedling development. We then focused on the aquaporin of maize tonoplast intrinsic protein 3 (ZmTIP3), which is specifically expressed in germinating seed. A mutant zmtip3-1 with disruption of the ZmTIP3-1 gene showed shorter shoot and root length, and decreased seedling dry weight compared with the control (W22). The result revealed that ZmTIP3-1 improved the absolute content of seed protein and promoted storage reserves mobilization, suggesting that ZmTIP3 may be a positive regulator of seed vigor. This work provides valuable clues for understanding the function and possible regulatory mechanism of ZmAQPs in seed germination and seedling growth.

Transcriptome analysis reveals the mechanism of internode development affecting maize stalk strength.

BACKGROUND: The stalk rind is one of the important factors affecting maize stalk strength that is closely related to stalk lodging. However, the mechanism of rind development in maize is still largely unknown. RESULTS: In this study, we analyzed the mechanical, anatomical, and biochemical properties of the third basal internode in one maize non-stiff-stalk (NSS) line and two stiff-stalk (SS) lines. Compared with the NSS line, the two SS lines had a significantly higher rind penetrometer resistance, thicker rind, and higher dry matter, hemicellulose, cellulose, and lignin weights per unit length. RNA-seq analysis was used to compare transcriptomes of the third basal internode of the two SS lines and the NSS line at the ninth leaf and tasseling stages. Gene Ontology (GO) enrichment analysis revealed that genes involved in hydrolase activity (hydrolyzing O-glycosyl compounds) and cytoskeleton organization were significantly up-regulated in the two SS lines at the ninth leaf stage and that microtubule process-related genes were significantly up-regulated in the two SS lines at the tasseling stage. Moreover, the two SS lines had enhanced expression of cell wall metabolism-related genes at the tasseling stage. CONCLUSIONS: The synthesis of cell wall polysaccharides and the cytoskeleton might play important roles in internode development. Our results can be applied for screening lodging-resistant inbred lines and breeding lodging-resistant cultivars in maize.

Modeling Dust in East Asia by CESM and Sources of Biases.

East Asian dust has a significant impact on regional and global climate. In this study, we evaluate the spatial distributions and temporal variations of dust extinction profiles and dust optical depth (DOD) over East Asia simulated from the Community Earth System Model (CESM) with satellite retrievals from Luo et al. (2015a, 2015b) (L15), Yu et al. (2015) (Y15), and Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) level 3 (CAL-L3) products. Both L15 and Y15 are based on CALIPSO products but use different algorithms to separate dust from non-dust aerosols. We find high model biases of dust extinction in the upper troposphere over the Taklamakan Desert, Gobi Desert, and Tibetan Plateau, especially in the summer (June-July-August, JJA). CESM with dust emission scheme of Kok et al. (2014a, 2014b) has the best agreement with dust extinction profiles and DOD from L15 in the Taklamakan Desert and Tibetan Plateau. CESM with the default dust emission scheme of Zender et al. (2003a) underpredicts DOD in the Tibetan Plateau compared with observations from L15 due to the underestimation of local dust emission. Large uncertainties exist in observations from L15, Y15, and CAL-L3 and have significant impacts on the model evaluation of dust spatial distributions. We also assess dust surface concentrations and 10 m wind speed with meteorological records from weather stations in the Taklamakan and Gobi Deserts during dust events. CESM underestimates dust surface concentrations at most weather stations due to the inability of CESM to capture strong surface wind events.

Effects of Nitrogen Level during Seed Production on Wheat Seed Vigor and Seedling Establishment at the Transcriptome Level.

Nitrogen fertilizer is a critical determinant of grain yield and seed quality in wheat. However, the mechanism of nitrogen level during seed production affecting wheat seed vigor and seedling establishment at the transcriptome level remains unknown. Here, we report that wheat seeds produced under different nitrogen levels (N0, N168, N240, and N300) showed significant differences in seed vigor and seedling establishment. In grain yield and seed vigor, N0 and N240 treatments showed the minimum and maximum, respectively. Subsequently, we used RNA-seq to analyze the transcriptomes of seeds and seedlings under N0 and N240 at the early stage of seedling establishment. Gene Ontology (GO) term enrichment analysis revealed that dioxygenase-activity-related genes were dramatically upregulated in faster growing seedlings. Among these genes, the top three involved linoleate 9S-lipoxygenase (Traes_2DL_D4BCDAA76, Traes_2DL_CE85DC5C0, and Traes_2DL_B5B62EE11). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that pathways involved in nutrient mobilization and the antioxidant system showed enhanced expression under N240. Moreover, seeds with faster growing seedlings had a higher gene expression level of α-amylase, which was consistent with α-amylase activity. Taken together, we propose a model for seedling establishment and seed vigor in response to nitrogen level during seed production.

Biomass smoke from southern Africa can significantly enhance the brightness of stratocumulus over the southeastern Atlantic Ocean.

Marine stratocumulus clouds cover nearly one-quarter of the ocean surface and thus play an extremely important role in determining the global radiative balance. The semipermanent marine stratocumulus deck over the southeastern Atlantic Ocean is of particular interest, because of its interactions with seasonal biomass burning aerosols that are emitted in southern Africa. Understanding the impacts of biomass burning aerosols on stratocumulus clouds and the implications for regional and global radiative balance is still very limited. Previous studies have focused on assessing the magnitude of the warming caused by solar scattering and absorption by biomass burning aerosols over stratocumulus (the direct radiative effect) or cloud adjustments to the direct radiative effect (the semidirect effect). Here, using a nested modeling approach in conjunction with observations from multiple satellites, we demonstrate that cloud condensation nuclei activated from biomass burning aerosols entrained into the stratocumulus (the microphysical effect) can play a dominant role in determining the total radiative forcing at the top of the atmosphere, compared with their direct and semidirect radiative effects. Biomass burning aerosols over the region and period with heavy loadings can cause a substantial cooling (daily mean -8.05 W m-2), primarily as a result of clouds brightening by reducing the cloud droplet size (the Twomey effect) and secondarily through modulating the diurnal cycle of cloud liquid water path and coverage (the cloud lifetime effect). Our results highlight the importance of realistically representing the interactions of stratocumulus with biomass burning aerosols in global climate models in this region.

Cloning and promoter activity analyses of the promoter of 2'-deoxymugineic acid (DMA) secretion channel gene YS3 in maize.

Iron (Fe) deficiency is a world-wide serious agricultural problem. Maize secretes 2'-deoxymugineic acid (DMA) to uptake and utilize Fe from the soil. In order to explore the gene expression patterns of the DMA secretion channel gene YS3, we cloned the 2813 bp YS3 promoter, and constructed the plant expression vector pCAMBIA-YS3GUS. The promoter contains a lot of TATA-boxes and CAAT-boxes, and cis-acting regulatory elements such as the light responsive elements and the hormone responsive elements. Arabidopsis was transformed via Agrobacterium tumefacients-mediated procedures to obtain the pYS3::GUS transgenic plants, which were confirmed by GUS staining. Then, the stained tissue was observed using paraffin section methods and the YS3 promoter activity was also analyzed. We found that the promoter could drive GUS gene expression specifically in the root epidermal cells. Mechanical damage could activate the promoter, and drive the GUS gene expression adjacent to the damage sites. Our results provide a molecular basis to understand the DMA secretion process in maize.

Pollen flow of wheat under natural conditions in the Huanghuai River Wheat Region, China.

The transgenic pollen spread is the main pathway of transgenic plant gene flow. The maximum distance of pollen dispersal (horizontal), the spatial dynamics of pollen movement (vertical), and the patterns of pollen dispersal are important considerations in biosafety assessments of genetically modified crops. To evaluate wheat (Triticum aestivum) pollen dispersal, we measured the pollen suspension velocity and analyzed pollen dispersal patterns under natural conditions in the Huanghuai River wheat-growing region in 2009. The pollen suspension velocity was 0.3-0.4 m/s. The highest pollen densities were detected in the north, northwest, and south of the pollen source. Pollen was dispersed over distances greater than 245 m in the northwest and northeast directions. At the pollen source center, pollen density decreased with increasing vertical height. In the north of the pollen source, the pollen density from 65 m to 225 m showed a wave-mode decrease with increasing height. The horizontal transport of pollen over longer distances fitted polynomial equations. In the north, the pollen density was highest at 45 m from the pollen source, and decreased with increasing distance. In the northwest, the pollen density showed a double-peak trend. In the northeast, pollen density was highest from 45 m to 125 m from the source. Wind speeds greater than the pollen suspension velocity and the duration of continuous gusts were the main factors affecting pollen dispersal. This information will be useful for determining the spatial isolation distances for hybrid seed production and for the commercial production of transgenic wheat.