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Traditional methods for algae removal in drinking water treatment, such as coagulation and sedimentation, face challenges due to the negative charge on algae cells' surfaces, resulting in ineffective removal. Ultrasonic cavitation has shown promise in enhancing coagulation performance by disrupting extracellular polymer structures and improving cyanobacteria removal through various mechanisms like shear force and free radical reactions. However, the short lifespan and limited mass transfer distance of free radicals in conventional ultrasonic treatment lead to high energy consumption, limiting widespread application. To overcome these limitations and enhance energy efficiency, advanced carbon-based materials were developed and tested. Nitrogen-doped functional groups on nanodiamond surfaces were found to boost sonosensitivity by increasing the production of reactive oxygen species at the sonosensitizer-water interface. Utilizing low-power ultrasound (0.12 W/mL) in combination with N-ND treatment for 5 min, removal rates of Microcystis aeruginosa cells in water exceeded 90 %, with enhanced removal of algal organic matters and microcystins in water. Visualization through confocal microscopy highlighted the role of positively charged nitrogen-doped nanodiamonds in aggregating algae cells. The synergy between cell capturing and catalysis of N-ND indicates that efficient mass transfer of free radicals from the sonosensitizer's surface to the microalgae's surface is critical for promoting cyanobacteria floc formation. This study underscores the potential of employing a low-intensity ultrasound and N-ND system in effectively improving algae removal in water treatment processes.
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Microcystis , Nanodiamantes , Nitrogênio , Nanodiamantes/química , Nitrogênio/química , Purificação da Água/métodosRESUMO
As a cost-effective material, biochar, known as 'black gold', has been widely used for environmental applications (EA), including chromium-contaminated wastewater remediation. However, limited reports focused on the multiple impacts of biochar, including energy consumption (EC) and environmental risk (ER). Hence, to recommend biochar as a green material for sustainable development, the three critical units were explored and quantitatively assessed based on an adapted 3E model (EA-EC-ER). The tested biochar was produced by limited-oxygen pyrolysis at 400-700 °C by using three typical biomasses (Ulva prolifera, phoenix tree, and municipal sludge), and the optimal operational modulus of the 3E model was identified using six key indicators. The findings revealed a significant positive correlation between EC and biochar yield (p < 0.05). The biochar produced by phoenix tree consumed more energy due to having higher content of unstable carbon fractions. Further, high-temperature and low-temperature biochar demonstrated different chromium removal mechanisms. Notably, the biochar produced at low temperature (400 °C) achieved better EA due to having high removal capacity and stability. Regarding ER, pyrolysis temperature of 500 °C could effectively stabilize the ecological risk in all biochar and the biochar produced by Ulva prolifera depicted the greatest reduction (37-fold). However, the increase in pyrolysis temperature would lead to an increase in global warming potential by nearly 22 times. Finally, the 3E model disclosed that the biochar produced by Ulva prolifera at 500 °C with low EC, high EA, and low ER had the most positive recommendation index (+78%). Importantly, a rapid assessment methodology was established by extracting parameters from the correlation. Based on this methodology, about eight percent of biochar can be the highest recommended from more than 100 collected peer-related data. Overall, the obtained findings highlighted that the multiple impacts of biochar should be considered to efficiently advance global sustainable development goals.
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Cromo , Algas Comestíveis , Ulva , Águas Residuárias , Carvão VegetalRESUMO
Sugarcane (Saccharum spp.), a leading sugar and energy crop, is seriously impacted by drought stress. However, the molecular mechanisms underlying sugarcane drought resistance, especially the functions of epigenetic regulators, remain elusive. Here, we show that a S. spontaneum KDM4/JHDM3 group JmjC protein, SsJMJ4, negatively regulates drought-stress responses through its H3K27me3 demethylase activity. Ectopic overexpression of SsJMJ4 in Arabidopsis reduced drought resistance possibly by promoting expression of AtWRKY54 and AtWRKY70, encoding two negative regulators of drought stress. SsJMJ4 directly bound to AtWRKY54 and AtWRKY70, and reduced H3K27me3 levels at these loci to ensure their proper transcription under normal conditions. Drought stress down-regulated both transcription and protein abundance of SsJMJ4, which was correlated with the reduced occupancy of SsJMJ4 at AtWRKY54 and AtWRKY70 chromatin, increased H3K27me3 levels at these loci, as well as reduced transcription levels of these genes. In S. spontaneum, drought stress-repressed transcription of SsWRKY122, an ortholog of AtWRKY54 and AtWRKY70, was associated with increased H3K27me3 levels at these loci. Transient overexpression of SsJMJ4 in S. spontaneum protoplasts raised transcription of SsWRKY122, paralleled with reduced H3K27me3 levels at its loci. These results suggest that the SsJMJ4-mediated dynamic deposition of H3K27me3 is required for an appropriate response to drought stress.
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Secas , Proteínas de Plantas , Saccharum , Saccharum/genética , Saccharum/fisiologia , Saccharum/metabolismo , Saccharum/enzimologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas , Estresse Fisiológico , Arabidopsis/genética , Arabidopsis/fisiologia , Histona Desmetilases/metabolismo , Histona Desmetilases/genética , Histonas/metabolismo , Histonas/genéticaRESUMO
Ultrasonic cavitation, characterized by the oscillation or abrupt collapse of cavitation nuclei in response to ultrasound stimulation, plays a significant role in various applications within both industrial and biomedical sectors. In particular, inertial cavitation (IC) has garnered considerable attention due to the resulting mechanical, chemical, and thermal effects. Passive cavitation detection (PCD) has emerged as a valuable technique for monitoring this procedure. While the fast Fourier transform (FFT) is a widely used algorithm to analyze IC-induced broadband noise detected by PCD system, it may not adequately capture the time-varying instability of cavitation due to potential nuclei collapse during ultrasound irradiation. In contrast, the continuous wavelet transform offers a more flexible approach, enabling more sensitive analysis of signals with varying frequencies over time. In this study, nanodiamond (ND) and its derivative, nitro-doped nanodiamond (N-AND), known to possess cavitation potential from previous research, were chosen as the source of cavitation nuclei. The cavitation signals detected by PCD were subjected to both FFT and wavelet analyses, with their results comprehensively compared. This research showcased the feasibility of employing wavelet analysis for effective inertial cavitation evaluation. It provided the advantage of monitoring the temporal evolution of cavitation events in real-time, enhancing sensitivity to weak and unstable cavitation signals, especially those in higher order components (3rd and 4th order). Additionally, it yielded a higher level of precision in determining IC thresholds and doses. Furthermore, the inclusion of time information through wavelet analysis offered insights into the limitations of low-cycle ultrasound in inducing IC. This study introduces a novel perspective for more sensitive and precise cavitation assessment, leveraging time and frequency data from wavelet analysis, and holds promise for effective utilization of cavitation effects while minimizing losses and damages resulting from unintended cavitation events.
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Bi2O2CO3(BOC)/Bi4O5Br2(BOB)/reduced graphene oxide (rGO) Z-scheme heterojunction with promising photocatalytic properties was synthesized via a facile one-pot room-temperature method. Ultra-thin nanosheets of BOC and BOB were grown in situ on rGO. The formed 2D/2D direct Z-scheme heterojunction of BOC/BOB with oxygen vacancies (OVs) effectively leads to lower negative electron reduction potential of BOB as well as higher positive hole oxidation potential of BOC, showing improved reduction/oxidation ability. Particularly, rGO is an acceptor of the electrons from the conduction band of BOC. Its dual roles significantly improve the transfer performance of photo-induced charge carriers and accelerate their separation. With layered nanosheet structure, rich OVs, high specific surface area, and increased utilization efficiency of visible light, the multiple synergistic effects of BOC/BOB/rGO can achieve effective generation and separation of the electron-holes, thereby generating more â¢O2- and h+. The photocatalytic reduction efficiency of CO2 to CO (12.91 µmol/(g·hr)) is three times higher than that of BOC (4.18 µmol/(g·hr)). Moreover, it also achieved almost 100% removal of Rhodamine B and cyanobacterial cells within 2 hours.
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Elétrons , Grafite , Temperatura , OxigênioRESUMO
In biomedical research, ultrasonic cavitation, especially inertial cavitation (IC) has attracted extensive attentions due to its ability to induce mechanical, chemical and thermal effects. Like ultrasound contrast agent (UCA) microbubbles or droplets, acoustic cavitation can be effectively triggered beyond a certain pressure threshold through the interaction between ultrasound and nucleation particles, leading to an enhanced thrombolytic effect. As a newly developed nanocarbon material, nitrogen-doped annealed nanodiamond (N-AND) has shown promising catalytic performance. To further explore its effects on ultrasonic cavitation, N-AND was synthesized at the temperature of 1000 °C. After systematic material characterization, the potential of N-AND to induce enhanced IC activity was assessed for the first time by using passive cavitation detection (PCD). Based on experiments performed at varied material suspension concentration and cycle number, N-AND demonstrated a strong capability to generate significant cavitation characteristics, indicating the formation of stable bubbles from the surface of the materials. Furthermore, N-AND was applied in the in vitro thrombolysis experiments to verify its contribution to ultrasound thrombolysis. The influence of surface hydrophobicity on the cavitation potentials of ND and N-AND was innovatively discussed in combination with the theory of mote-induced nucleation. It is found that the cavitation stability of N-AND was better than that of the commercial UCA microbubbles. This study would provide better understanding of the potential of novel carbonous nanomaterials as cavitation nuclei and is expected to provide guidance for their future biomedical and industrial applications.
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1000 kHz high-frequency ultrasound at 0.12 and 0.39 W/mL intensity was used to enhance the inactivation of suspensions of Microcystis aeruginosa cells using KMnO4. With 10 mg/L of KMnO4, ultrasound at 0.12 W/mL intensity was found to be effective in inactivating the cyanobacteria within 10 min. A Weibull model was found to describes the inactivation well. Its concave shape shows that some cells have a certain resistance to this treatment. Cytometry and microscopic analysis confirm that the treatment damages cell integrity. Despite that the extracellular organic matter in the water was not significantly increased. The concentration of extracellular cyanobacterial toxins even decreased. The filtered suspension of inactivated cyanobacteria was used to cultivate mung beans, and the suspension did not hinder their germination. This provides a new idea for using cyanobacteria-laden wastewater. These findings suggest a technique for speeding up the oxidation of Microcystis cells using KMnO4 with ultrasound at moderate intensity, which provide new insights into the biological effects of ultrasound.
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Cianobactérias , Microcystis , Purificação da Água , Permanganato de Potássio/farmacologia , Oxirredução , Purificação da Água/métodosRESUMO
Phase-changeable contrast agents have been proposed as a next-generation ultrasound contrast agent over conventional microbubbles given its stability, longer circulation time and ability to extravasate. Safe vaporization of nanodroplets (NDs) plays an essential role in the practical translation of ND applications in industry and medical therapy. In particular, the exposure parameters for initializing phase change as well as the site of phase change are concerned to be controlled. Compared to the traditional optical vaporization or acoustic droplet vaporization, this study exhibited the potential of using simultaneous, single burst laser and ultrasound incidence as a means of activating phase change of NDs to generate cavitation nuclei with reduced fluence and sound pressure. A theoretical model considering the laser heating, vapor cavity nucleation and growth was established, where qualitative agreement with experiment findings were found in terms of the trend of combined exposure parameters in order to achieve the same level of vaporization outcome. The results indicate that using single burst laser pulse and 10-cycle ultrasound might be sufficient to lower the exposure levels under FDA limit for laser skin exposure and ultrasound imaging. The combination of laser and ultrasound also provides temporal and spatial control of ND vaporization and cavitation nucleation without altering the sound field, which is beneficial for further safe and effective applications of phase-changeable NDs in medical, environmental, food processing and other industrial areas.
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Acústica , Som , Volatilização , Ultrassonografia , Meios de Contraste , Lasers , MicrobolhasRESUMO
Biochar has been widely used in the fields of environment and energy, and green preparation can make biochar-based materials more environmentally friendly. Particularly, in the low-temperature pyrolysis of biochar, labile C with low biological toxicity is the main influencing factor of bacteria in soil. Therefore, it is worth studying to develop the fabrication technology of low-temperature pyrolysis biochar with rich pore structure. The mechanical effect of ultrasonic cavitation is considered to be an effective strategy for the preparation of biochar. However, the sonochemical effects on biochar remain to be studied. In this review, ultrasonic modification and ultrasonic-chemical modification on biochar has been reviewed. Metal oxide/biochar composites can also be obtained by an ultrasonic-chemical method. It is worth mentioning that there have been some reports on the regeneration of biochar by ultrasound. In addition to ultrasonic preparation of biochar, ultrasound can also trigger the sonocatalytic performance and promote the adsorption ability of biochar for the removal of harmful substances. The catalytic mechanism of ultrasound/biochar needs to be further investigated. For application, biochar prepared by ultrasound has been used for the removal of heavy metals in water, the adsorption of carbon dioxide, and soil remediation.
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Suspended particulate matter (SPM) and sediments are important sources of dissolved organic matter (DOM) in lake water. However, studies on what extent and how both sources affect DOM composition are lacking, which hampers DOM management. Herein, DOM, SPM-extracted particulate organic matter (POM), and sediment-extracted organic matter (SOM) were characterized and compared in terms of absorption spectral properties and chemical composition in Lake Taihu, a large cyanobacterial bloom-affected shallow lake. A statistical method was proposed to quantify the similarity of organic matter (OM) in the different states and to evaluate the potential effects of SPM and sediments on DOM. Results showed that POM and DOM were mainly composed of small-molecular-size and low-humified organic components (i.e., 27 %-38 % tryptophan-like and ~30 % protein-like substances), and most of them were derived from autochthonous sources. While tyrosine-like (57 %) and humic-like (27 %) substances were dominant in SOM. The OM similarity between POM and DOM was approximately 1.5 times higher than that between SOM and DOM, indicating the greater effect of SPM than sediments on DOM composition. High pH and low nitrogen (e.g., nitrate and ammonia) were positively correlated to the OM similarity between POM and DOM. Further, the findings indicated that nitrogen limitation enhanced the OM exchange between POM and DOM by promoting the production of extracellular polymeric substances (EPS) in cyanobacterial aggregates. The obtained findings highlighted the importance of SPM in shaping the DOM composition relative to sediments and facilitating the DOM management in bloom-affected lakes.
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Cianobactérias , Lagos , China , Matéria Orgânica Dissolvida , Substâncias Húmicas/análise , Lagos/química , Nitrogênio , Material Particulado/análise , ÁguaRESUMO
Introduction: Semiconductors have similar crystal structures and matched energy levels could form a coupled heterojunction at an interface between them which may allow response to visible light, achieving efficient decomposition of organic compounds. Methods: The Bi2O2CO3/BiOI (BOC/BOI) with 2D/2D p-n heterojunction was prepared by one-pot room-temperature strategy. The prepared materials were tested by various technologies, and the three-dimensional structure, light absorption properties, electrochemical properties and other information were obtained. Photocatalytic tests have also been carried out. Results and discussion: BOC/BOI heterojunction with oxygen vacancies showed much higher photocatalytic activity than pure BOC and BOI. For example, the preferred BOC/BOI-0.5 heterojunction of the degradation rate for Rhodamine B (RhB) is 97.6 % within 2 h, which is 15.8 and 2.2 times faster than that of BiOI and BOC. In addition, the removal rates of tetracycline, ciprofloxacin and bisphenol A by BOC/ BOI-0.5 were 92.4, 80.3 and 68.6%, respectively. The 2D/2D structures of BOC/BOI-0.5 with rich in oxygen vacancies combined p-n junction can effectively inhibit the photoinduced electron-hole pair recombination and increase the production of active free radicals. The O2- and h+ are the main reactants, giving the composite catalyst potential for degrading a variety of pollutants.
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The optimization of volume ratio (VAn/VA/VO) and nitrate recycling ratio (R) in a two-sludge denitrifying phosphorus removal (DPR) process of Anaerobic Anoxic Oxic-Moving Bed Biofilm Reactor (A2/O-MBBR) was investigated. The results showed that prolonged anaerobic retention time (HRTAn: 1.25â3.75 hr) exerted favorable effect on chemical oxygen demand (COD) removal (57.26%â73.54%), poly-ß-hydroxyalkanoates (PHA) synthesis (105.70â138.12 mgCOD/L) and PO43- release (22.3â38.9 mg/L). However, anoxic retention time (HRTA) and R exhibited positive correlation with PHA utilization (43.87%-81.34%) and denitrifying phosphorus removal (DPR) potential (ΔNO3-/ΔPO43-: 0.57-1.34 mg/mg), leading to dramatical TN removal variations from 68.86% to 81.28%. Under the VAn/VA/VO ratio of 2:6:0, sludge loss deteriorated nutrient removals but the sludge bioactivity quickly recovered when the oxic zone was recovered. The sludge characteristic and microstructure gradually transformed under the dissolved oxygen (DO) control (1.0-1.5â1.5-2.0 mg/L), in terms of sludge volume index (SVI: 194â57 mL/gVSS), median-particle-size (D50: 99.6â300.5 µm), extracellular polymeric substances (EPS) (105.62â226.18 mg/g VSS) and proteins/polysaccharides (PN/PS) ratio (1.52â3.46). Fluorescence in situ hybridization (FISH) results showed that phosphorus accumulation organisms (PAOs) (mainly Cluster I of Accumulibacter, contribution ratio: 91.79%-94.10%) dominated the superior DPR performance, while glycogen accumulating organisms (GAOs) (mainly Competibacter, contribution ratio: 82.61%-86.89%) was responsible for deteriorative TN and PO43- removals. The optimal HRTA and R assembled around 5-6.5 hr and 300%-400% based on the PHA utilization and DRP performance, and the oxic zones also contributed to PO43- removal although it showed low dependence on DO concentration and oxic retention time (HRTO).
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Fósforo , Esgotos , Biofilmes , Reatores Biológicos , Desnitrificação , Hibridização in Situ Fluorescente , Nitratos , Nitrogênio , Nutrientes , Eliminação de Resíduos LíquidosRESUMO
Harmful cyanobacteria blooms are increasing. They call for novel removal technology, since the required doses of algaecides may cause further environmental pollution or damage treatment facilities. Undesirable intracellular compounds can be released in the water when cyanobacterial cells are damaged. For the first time, ultrasound irradiation was combined with TiO2/biochar (TiO2/BC) at relatively low dosage and tested as an alternative for promoting the coagulation of Microcystis aeruginosa in water treatment. This pre-oxidation process removed 92% of cyanobacterial cells after coagulation. With the combination of ultrasound and TiO2/BC treatment, the dissolved organic carbon and microcystins levels did not increase significantly. The oxidative treatments enhanced the permeability of the cyanobacterial cell membranes, which may be due to the various active species generated from the ultrasound and TiO2/BC process. The results showed that the TiO2/BC hybrid catalyst could be a potential candidate for cyanobacterial cells removal in water.
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Harmful cyanobacterial blooms are increasing environmental issues and require novel removal technology since the required doses of algaecides may cause further environmental pollution or treatment facility damage. Herein, we firstly introduce the combination of ultrasound and Fe3O4/CNTs as an alternative strategy to enhance coagulation for the removal of Microcystis aeruginosa cells in water. It remarkably enhanced cyanobacterial cell removal and microcystins control, compared with sonication alone (40 kHz ultrasonic bath, 4.2 mJ mL-1). 94.4% cyanobacterial cells were removed using 20 second sonication with 20 mg L-1 Fe3O4/CNTs, Al2(SO4)3 coagulation (20 µM). Both sonication time and catalyst dose significantly influenced the cyanobacterial removal. Ultrasound with Fe3O4/CNTs only induced a slight increase of cell permeability, which may contribute to the effective control of DOC and microcystins' release in water. The enhanced settlement of the cyanobacterial cells may result from the moderate oxidation on the cell surface. This study suggested a novel ultrasound-Fe3O4/CNT process to promote cyanobacteria removal with efficient DOC and microcystin release control, which is a green and safe technology for drinking water treatment.
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Long-term administration of chemotherapeutic agents often leads to multiple drug resistance (MDR), which greatly impairs the treatment outcome. To overcome this problem, a biodegradable nanocarrier based on an acid-sensitive calcium phosphate/silica dioxide (CAP/SiO2) composite was constructed for the codelivery of drug and siRNA. Anticancer drug doxorubicin (DOX) was encapsulated into the composite scaffold by interacting with the exposed Ca2+ of CAP/SiO2 to achieve high drug loading (180 µg mg-1). With further decoration of siRNA, the nanocarrier was applied to enhance the therapeutic efficacy by silencing MDR-relevant genes (P-gp) of DOX-resistance K562/ADR cancer cells. Benefiting from the intrinsic acid degradability of CAP/SiO2, the nanocomposite demonstrated pH-responsive release behavior, favoring drug/siRNA release within acidic endo-/lysosomes. Consequently, due to the drug and gene effects, this biodegradable nanomedicine demonstrated enhanced therapeutic efficiency, providing a novel strategy for cancer therapy.
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Ultra-thin layered structures and modified bandgaps are two efficient strategies to increase the photocatalytic performance of various materials for the semiconductor industry. In the present study, we combined both strategies in one material to form carbon-doped graphitic carbon nitride (g-C3N4) nano-layered structures by the method of melamine thermal condensation, in the presence of different mass ratios of biochar. The characterization showed that the composite with the best ratio retained the g-C3N4 polymeric framework and the bond with g-C3N4. The biochar was established via π-π stacking interactions and ether bond bridges. The π-conjugated electron systems provided from biochar can elevate charge separation efficiency. The ultra-thin structure also curtailed the distance of photogenerated electrons migrating to the surface and enlarge specific surface area of materials. The presence of carbon narrowed the bandgap and increased light absorption at a wider range of wavelengths of g-C3N4. The biochar/melamine ratio of 1:15 presented the best performance, 2.8 and 5 times faster than g-C3N4 degradating Rhodamine and Methyl Orange, respectively. Moreover, the catalyst presented a good stability for 4 cycles. In addition to that, biochar from waste biomass can be considered a sustainable, cost-effective, and efficient option to modify g-C3N4-based photocatalysts.
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Carvão Vegetal/farmacologia , Grafite/química , Luz , Nitrilas/química , Compostos de Nitrogênio/química , Fotólise/efeitos dos fármacos , Compostos Azo/química , Catálise , Carvão Vegetal/química , Rodaminas/químicaRESUMO
Iron oxide nanoparticles decorated on multi-wall nanotube (MWCNTs) were successfully fabricated through a facile and rapid sonochemical method without any pre-treatment on MWCNTs. Fe3O4/MWCNTs-20 showed a uniform and fine distribution of nanoparticles in the MWCNTs. The obtained Fe3O4/MWCNTs were analysed using TEM and XPS. Notably, Fe3O4/MWCNTs were used for persulfate activation on cyanobacterial cell removal. With 20â¯mg/L persulfate, Fe3O4/MWCNTs showed an efficient catalytic performance after 1â¯h treatment. In the Fe3O4/MWCNTs hybrid catalyst, Fe3O4 helps to produce sulfate radicals and hydroxyl radicals whereas the size of the Fe3O4 clusters could affect the electron transfer for radical generation. Moreover, using high frequency low intensity ultrasound, the combination of persulfate and Fe3O4/MWCNTs-20 reduced the remaining cell number to 9.4% within 30â¯min treatment. In conclusion, our work demonstrated that low frequency ultrasonic devices are capable of fabricating Fe3O4/MWCNTs via a simple and time-saving route, and the obtained catalysts showed superior catalytic performance on persulfate for harmful cyanobacteria control.
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Nanopartículas de Magnetita/química , Viabilidade Microbiana/efeitos dos fármacos , Microcystis/efeitos dos fármacos , Microcystis/fisiologia , Nanotubos de Carbono/química , Sulfatos/química , Ondas Ultrassônicas , Catálise , Técnicas de Química SintéticaRESUMO
For the first time, the inactivation of Microcystis aeruginosa using sono-Fenton process at low frequency high intensity (20â¯kHz, 0.42â¯W/mL) and high frequency low intensity (800â¯kHz, 0.07â¯W/mL) was investigated, respectively. 20â¯kHz sono-Fenton treatment successfully reduced cyanobacterial cell number from 4.19â¯×â¯106â¯cells/mL to 0.45â¯×â¯106â¯cells/mL within 5â¯min treatment. Alternatively, efficient performance of 800â¯kHz sono-Fenton process was observed to decrease Microcystis cell number to 2.33â¯×â¯106â¯cells/mL after 5â¯min inactivation, with lower energy cost. It was found that powerful 20â¯kHz sonication induced pore formation on the cell wall, leading to extracellular damage, while 800â¯kHz irradiation with low intensity triggered intracellular uptake of chemicals, suggesting endocytosis effects. Furthermore, sono-Fenton Processes were found to be affected by the concentrations of Fenton's reagent, and pre-sonication time. Although solo Fenton treatment released microcystins in water, the degradation of microcystin-LR were achieved using 20 and 800â¯kHz sono-Fenton processes, respectively. The results of this work showed that severe extracellular oxidation is the vital inactivation mechanism of 20â¯kHz sono-Fenton process, while the internal oxidation caused by intracellularly delivered Fenton reagents is suggested to be the main cause of 800â¯kHz sono-Fenton inactivation, leading to much lower energy cost. This work provides alternative methods to control harmful cyanobacteria in water towards effective treatment.