Europium-Doped 3D Dimensional Porous Calcium Phosphate Scaffolds as a Strategy for Facilitating the Comprehensive Regeneration of Bone Tissue: In Vitro and In Vivo.

In response to the challenges faced by clinicians treating bone defects caused by various factors, various bone repair materials have been investigated, but the efficiency of bone healing still needs to be improved due to the acting of scaffolds only in a single stage of bone tissue regeneration. We investigated the potential of a novel 3D scaffold to support different stages of bone tissue regeneration, including initial inflammation, proliferation, and remodeling. Eu (0, 0.5, 2, 3.5, 5, and 6.5%) was added to calcium polyphosphate to obtain 3D porous network-doped Eu calcium polyphosphate (EuCPP) scaffolds with ideal mechanical strength and pore size. Both in vitro and in vivo experiments proved that Eu3+ released from 5% EuCPP scaffolds could significantly promote the migration and proliferation of bone marrow stromal cells which effectively promote angiogenesis; 5% EuCPP could significantly upregulate the ratio of OPG/RANKL in MC3T3-E1 and promote the secretion of osteogenic-related growth factors (ALP and OPN) from MC3T3-E1, indicating the potential of the scaffold to inhibit bone resorption and promote bone formation. In conclusion, 5% EuCPP possesses the biological properties of pro-angiogenesis, anti-inflammation, pro-osteogenesis, and inhibiting bone resorption, which may provide a sustained positive effect throughout the process of bone tissue repair.

Enhancing the selective synthesis of butyrate in microbial electrosynthesis system by gas diffusion membrane composite biocathode.

The reduction of carbon dioxide (CO2) to high value-added multi-carbon compounds at the cathode is an emerging application of microbial electrosynthesis system (MES). In this study, a composite cathode consisting of hollow fiber membrane (HFM) and the carbon felt is designed to enhance the CO2 mass transfer of the cathode. The result shows that the main products are acetate and butyrate without other substances. The electrochemical performance of the electrode is significantly improved after biofilm becomes matures. The composite cathode significantly reduces the "threshold" for the synthesis of butyrate. Moreover, CO2 is dissolved and protons are consumed by synthesizing volatile fatty acids (VFAs) to maintain a stable pH inside the composite electrode. The synthesis mechanism of butyrate is that CO2 is converted sequentially into acetate and butyrate. The microenvironment of the composite electrode enriches Firmicute. This composite electrode provides a novel strategy for regulating the microenvironment.

Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.

Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate. In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio. The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability. Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis. What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel. It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair. All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.