Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman × Thai native crossbreed cattle.

Background and Aims: Lipopolysaccharide (LPS) is a robust endotoxin known to activate the immune system in cattle. The objective of this study was to investigate the effect of LPS on the morphology, cell viability, malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC) of peripheral blood mononuclear cells (PBMCs) in Brahman and Brahman × Thai native crossbreed cattle. Materials and Methods: PBMCs were isolated from Brahman and Brahman × Thai native crossbreed cattle and treated with 0, 0.1, 1, and 10 µg/mL Escherichia coli LPS, respectively. Morphological changes in PBMCs were assessed at 24 and 48 h. In addition, we measured PBMC cell viability, MDA, NO, and TAC. Results: LPS stimulation caused cell deformation and partial PBMC area enlargement, but there were no differences between Brahman and Brahman × Thai native crossbreed cattle. Stimulation at all levels did not affect the viability of PBMCs (p > 0.05). MDA and NO levels were significantly higher in Brahman cattle than in Brahman Thai native crossbred cattle (p < 0.05). TAC was significantly higher in Brahman × Thai native crossbred cattle than in Brahman cattle (p < 0.05). Conclusion: Immune cells of crossbreed cattle have a higher activation response to LPS than those of purebred cattle, and native crossbreed beef cattle have a higher antioxidant capacity than purebred beef cattle. This result may explain why hybrid cattle of indigenous breeds are more resistant to disease than purebred cattle.

Helminth-induced impairment of humoral immunity differently contribute to their anti-arthritic effects in mice: Comparison of Schistosoma mansoni and Trichinella spiralis.

AIMS: We have previously reported reduction of anti-type II collagen (IIC) IgG levels in collagen-induced arthritis (CIA) by Schistosoma mansoni (Sm) and Trichinella spiralis (Ts). To clarify the contribution of the impairment of humoral immunity to their anti-arthritic activities, we herein investigated the relationship between anti-IIC IgG levels and arthritic swelling in Sm- or Ts-infected mice. METHODS AND RESULTS: Male DBA/1J mice were infected with Sm cercariae or Ts muscle larvae prior to the IIC immunization. In the Sm-infected mice, paw swelling and anti-IIC IgG levels were continuously lower than those of non-infected control group. In contrast, arthritic swelling in the Ts-infected mice only decreased in the early phase of CIA progression, despite the continued impairment of anti-IIC IgG production throughout the experimental period. Correlation coefficients between residual paw swelling and anti-IIC IgG titers were similar or higher in the Sm group than in the control group, but were similar or lower in the Ts group than in the control group. CONCLUSION: The down-modulations of anti-IIC IgG levels by the two parasitic infections and the correlation analyses suggest that the anti-arthritic activity of Sm was primarily attributed to the modulation of IgG-independent arthritogenic mechanisms and secondarily to the impairment of anti-IIC IgG production. In contrast, Ts could alleviate CIA mainly via the impairment of antibody production.

Robust Audio-Visual Contrastive Learning for Proposal-based Self-supervised Sound Source Localization in Videos.

By observing a scene and listening to corresponding audio cues, humans can easily recognize where the sound is. To achieve such cross-modal perception on machines, existing methods take advantage of the maps obtained by interpolation operations to localize the sound source. As semantic object-level localization is more attractive for prospective practical applications, we argue that these map-based methods only offer a coarse-grained and indirect description of the sound source. Additionally, these methods utilize a single audio-visual tuple at a time during self-supervised learning, causing the model to lose the crucial chance to reason about the data distribution of large-scale audio-visual samples. Although the introduction of Audio-Visual Contrastive Learning (AVCL) can effectively alleviate this issue, the contrastive set constructed by randomly sampling is based on the assumption that the audio and visual segments from all other videos are not semantically related. Since the resulting contrastive set contains a large number of faulty negatives, we believe that this assumption is rough. In this paper, we advocate a novel proposal-based solution that directly localizes the semantic object-level sound source, without any manual annotations. The Global Response Map (GRM) is incorporated as an unsupervised spatial constraint to filter those instances corresponding to a large number of sound-unrelated regions. As a result, our proposal-based Sound Source Localization (SSL) can be cast into a simpler Multiple Instance Learning (MIL) problem. To overcome the limitation of random sampling in AVCL, we propose a novel Active Contrastive Set Mining (ACSM) to mine the contrastive sets with informative and diverse negatives for robust AVCL. Our approaches achieve state-of-the-art (SOTA) performance when compared to several baselines on multiple SSL datasets with diverse scenarios.

Simultaneous Efficient Photocatalytic Hydrogen Evolution and Degradation of Dye Wastewater without Cocatalysts and Sacrificial Agents Based on g-C3 N5 and Hybridized Ni-MOF Derivative-CdS-DETA.

Inspired by energy conversion and waste reuse, hybridized Ni-MOF derivative-CdS-DETA/g-C3 N5 , a type-II heterojunction photocatalyst, is synthesized by a hydrothermal method for simultaneous and highly efficient photocatalytic degradation and hydrogen evolution in dye wastewater. Without the addition of cocatalysts and sacrificial agents, the optimal MOF-CD(2)/CN5 (i.e. Ni-MOF derivative-CdS-DETA (20 wt.%)/g-C3 N5 ) exhibit good bifunctional catalytic activity, with a H2 evolution rate of 2974.4 µmol g-1  h-1 during the degradation of rhodamine B (RhB), and a removal rate of 99.97% for RhB. In the process of H2 -evolution-only, triethanolamine is used as a sacrificial agent, exhibiting a high H2 evolution rate (19663.1 µmol g-1  h-1 ) in the absence of a cocatalyst, and outperforming most similar related materials (such as MOF/g-C3 N5 , MOF-CdS, CdS/g-C3 N5 ). With the help of type-II heterojunction, holes are scavenged for the oxidative degradation of RhB, and electrons are used in the decomposition of water for H2 evolution during illumination. This work opens a new path for photocatalysts with dual functions of simultaneous efficient degradation and hydrogen evolution.

Identification of factors driving the spatial distribution of molybdenum (Mo) in topsoil in the Longitudinal Range-Gorge Region of Southwestern China using the Geodetector model.

As a key component of plant nitrogen-fixing enzymes and a variety of human coenzyme factors, molybdenum (Mo) plays an essential role in supporting both plant growth and human health. Soil is a key medium for the cycling of Mo in the biosphere. However, the driving anthropogenic and natural factors governing the spatial distribution of Mo in soil and their interactions are not well understood. To determine the factors that affect the spatial patterns of Mo in topsoil, 6980 samples were collected from the Longitudinal Range-Gorge Region (Linshui County, Sichuan Province, China). In this area, tall mountains are adjacent to deep valleys. Topsoil with enriched Mo is mostly distributed in mountainous areas. The most important factors influencing Mo in topsoil are soil parent materials (q = 0.482), altitude (q = 0.256), and soil type (q = 0.259). There are synergistic effects among the various driving factors [q(X1 âˆ© X2) > Max[q(X1), q(X2)]]. The Geodetector model was used to validate the magnitude of the interaction effects. The contribution to interacting factors is nonlinearly enhanced when the contribution of a single factor was low (any two factors of aspect, road distance, land use type, and S). The contribution to interacting factors is enhanced bidirectionally when the contribution of a single factor was high (any two factors of altitude, soil type, soil parent material, OM, and TFe2O3). When the contribution of one factor is high and the other is low, the contributing to interacting factors is mostly enhanced bidirectionally and a few are nonlinearly enhanced.

Detection of ivermectin and fipronil resistance in Rhipicephalus sanguineus sensu lato in Maha Sarakham, Thailand.

Background and Aim: Administration is the main strategy for controlling ectoparasites in dogs. Ivermectin and fipronil are most extensively used to prevent and treat Rhipicephalus sanguineus sensu lato infestation in dogs in Thailand. Several researchers have reported resistance to acaricides in R. sanguineus s.l. globally, but documentation of acaricide resistance in the tick population in Thailand is lacking. In this study, we investigated the status of resistance to commonly used acaricides in Thailand in R. sanguineus s.l. Materials and Methods: Engorged brown dog tick females (10 tick populations) were field-collected directly from parasitized dogs in Maha Sarakham, Thailand, for toxicological bioassays with ivermectin and fipronil. Bioassays were performed in three replicates at 25°C-27°C and 80%-85% relative humidity under a 12-h/12-h photoperiod. The 50% of lethal concentration and its confidence intervals and the slope were estimated for each tick population using probit analysis. Resistance ratios (RRs) of field ticks were characterized based on the relative susceptible population of each acaricide. Results: Six tick populations (P1-6) were tested for resistance to ivermectin, three of which (P2-4) exhibited low-level resistance to ivermectin (RR = 2.115-2.176). Of four tick populations (P7-10) treated with fipronil, two exhibited moderate-to-severe resistance (P7 and P9, RR = 21.684 and 4.387, respectively). All tick populations deemed resistant to acaricides had a history of exposure. Conclusion: Based on RR values, four R. sanguineus s.l. tick populations from Maha Sarakham province were resistant to ivermectin and fipronil. To the best of our knowledge, this represents the first documentation of acaricide-resistant populations of R. sanguineus s.l. in Thailand, and recommendations on tick control programs must be formulated with veterinarians and pet owners to prevent the development of further resistance.

The neuroprotective effects of FG-4592, a hypoxia-inducible factor-prolyl hydroxylase inhibitor, against oxidative stress induced by alpha-synuclein in N2a cells.

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra. The pathological hallmark of PD is the appearance of intraneuronal cytoplasmic α-synuclein (α-Syn) aggregation, called Lewy bodies. α-Syn aggregation is deeply involved in the pathogenesis of PD. Oxidative stress is also associated with the progression of PD. In the present study, to investigate whether a hypoxia-inducible factor (HIF)-prolyl hydroxylase (PH) inhibitor, FG-4592 (also called roxadustat), has neuroprotective effects against α-Syn-induced neurotoxicity, we employed a novel α-Syn stably expressing cell line (named α-Syn-N2a cells) utilizing a piggyBac transposon system. In α-Syn-N2a cells, oxidative stress and cell death were induced by α-Syn, and FG-4592 showed significant protection against this neurotoxicity. However, FG-4592 did not affect α-Syn protein levels. FG-4592 triggered heme oxygenase-1 (HO-1) expression downstream of HIF-1α in a concentration-dependent manner. In addition, FG-4592 decreased the production of reactive oxygen species possibly via the activation of HO-1 and subsequently suppressed α-Syn-induced neurotoxicity. Moreover, FG-4592 regulated mitochondrial biogenesis and respiration via the induction of the peroxisome proliferator-activated receptor-γ coactivator-1α. As FG-4592 has various neuroprotective effects against α-Syn and is involved in drug repositioning, it may have novel therapeutic potential for PD.

Carbonate rocks as natural buffers: Exploring their environmental impact on heavy metals in sulfide deposits.

Carbonate rocks are closely related to the genesis and spatial distribution of polymetallic sulfide deposits. The natural buffering of carbonate rocks can reduce the ecological impact of heavy metals produced by mining and smelting. Ignoring the buffering effect of carbonate rocks on the heavy metals in the mine environment leads to inaccurate ecological risk assessment, wasting land resources and funds. This study investigates Cd, Zn, and Pb distribution and speciation in the water and soil-rice system in the polymetallic sulfide deposit at Daxin, Guangxi. The study aims to reveal the effects of the natural buffering of carbonate rocks on the migration and transformation of heavy metals. The results show that the water Zn and Cd concentrations decreased from 1857.0 to 0.9 mg L-1 to 0.16 and 0.001 mg L-1, respectively, from the mining area to 4 km downstream. The natural buffering of carbonate increases the water pH from 2.80 to 7.64, resulting in a tendency for Cd, Zn, and Pb to separate from the aqueous phase and enrich the sediments. Soil Cd content in the mining area reached 110.0 mg kg-1 (mean value 55.88 mg kg-1), and rice Cd seriously exceeded the maximum limit. However, the weathering of carbonate reduces the migration ability and bioavailability of Cd. Soil Cd is mainly in the Fe-Mn bound and carbonate-bound fractions, resulting in lower Cd content in downstream soils (mean value 2.73 mg kg-1). Soil CaO, tFe2O3, and Mn hindered the uptake of soil Cd by rice rendering a lower exceedance of Cd in downstream rice. Therefore, this study recommends a farmland management plan under the premise of rice Cd content and integrated soil Cd content, which ensures food safety and fully utilizes farmland resources. This result provides a scientific basis for ecological risk assessment, mine environmental protection, and management in the carbonatite sulfide mine environment.

4-Methyl-5-vinyl thiazole modified Ni-MOF/g-C3N4/CdS composites for efficient photocatalytic hydrogen evolution without precious metal cocatalysts.

The construction of heterojunction systems is an effective way to efficiently generate hydrogen by water photolysis. In this work, Ni-MOF (trimesic acid, (BTC)) and g-C3N4 (denoted as CN) were combined, and then Ni-MOF/CN was modified by 4-Methyl-5-vinyl thiazole (denoted as MVTh). Finally, CdS was loaded on the surface of Ni-MOF/CN/MVTh to prepare the photocatalyst Ni-MOF/g-C3N4/MVTh/CdS (denoted as Ni/CN/M/Cd) with a triangular closed-loop path heterojunction for the first time. As a photocatalyst without precious metal cocatalysts, Ni/CN/M/Cd displayed high H2 evolution (17.844 mmol·g-1·h-1) under an optimum CdS loading of 40 wt%. The H2 evolution rate was approximately 79 times that of Ni-MOF/CN and exceeded those of almost all catalysts based on MOF/CN in the literature. The triangular closed-loop heterojunction formed between Ni-MOF, g-C3N4, and CdS could realize the directional migration of photocarriers and significantly diminished the transfer resistance of carriers. The Ni2+ in Ni-MOF provided many cocatalytic sites for H2 evolution via g-C3N4 and CdS. Furthermore, charge carrier separation in Ni-MOF/CN/CdS improved after the innovative addition of MVTh. This study provides a reference for the construction of a closed-loop heterojunction system without precious metal cocatalysts.

A High-Precision Multi-Beam Optical Measurement Method for Cylindrical Surface Profile.

To automatically measure the surface profile of a cylindrical workpiece, a high-precision multi-beam optical method is proposed in this paper. First, some successive images for the cylindrical workpiece's surface are acquired by a multi-beam angle sensor under different light directions. Then, the light directions are estimated based on the feature regions in the images to calculate surface normal vectors. Finally, according to the relationship of the surface normal vector and the vertical section of the workpiece's surface, a depth map is reconstructed to achieve the curvature surface, which can be employed to measure the curvature radius of the cylindrical workpiece's surface. Experimental results indicate that the proposed measurement method can achieve good measurement precision with a mean error of the curvature radius of a workpiece's surface of 0.89% at a reasonable speed of 10.226 s, which is superior to some existing methods.

Simulation and optimization of the thermal sterilization process of puree cans using the production of chestnut puree as an example.

In the production process of puree cans such as chestnuts cans, it is easy to browning due to excessive heating, which causes a lot of waste every year. The heat and mass transfer model of Chinese Chestnut Puree was established through the finite element method. The model simulated the change process of the temperature field, heat flow velocity field and F value during the production of Chinese Chestnut Puree. After comparing and confirming the effectiveness of the model through the thermal penetration test, the model was used to adjust and optimize the production process. For #9121 cans, the two-stage sterilization method was adopted. Through the sterilization method at 10-65-48-14/118-110°C, a sterilization effect equivalent to that of the original process at 10-86-24/121°C was achieved, the browning problem of the product was alleviated, and the product quality was improved. This practice can also provide a reference for canning enterprises to adjust their production processes in the future.

Enrichment mechanisms of Mo in soil in the karst region Guangxi, China.

Soils developed in karst regions have naturally high background values of molybdenum (Mo) due to geological factors. However, the enrichment mechanism of Mo in these soils are not fully understood, making it challenging to assess their ecological risk and utilize Mo-rich land resources. To shed light on this issue, this study collected and analyzed data from the 1:50,000 geochemical survey in Guangxi, including 536,503 sets of soil data and 3043 sets of rock data, as well as 40 sets of carbonate rock-soil from typical karst regions. The results showed that soil Mo enrichment is highly correlated with the distribution of carbonate rocks in karst regions. The carbonate rocks in these regions contain Mo ranging from 0.03 to 1.06 mg·kg-1 (with a mean of 0.22 mg·kg-1). In comparison, the soil Mo derived from carbonate rocks can reach up to 6.00 mg·kg-1 (with a mean of 2.75 mg·kg-1), representing an average enrichment of soil Mo that is 24 times higher compared to the carbonate parent rock. The enrichment of soil Mo in karst regions is primarily controlled by secondary enrichment during the weathering process of carbonate. During the insoluble residue accumulation process, the dissolution of carbonate leads to a dramatic reduction in bedrock volume, and the adsorption of clay minerals and Fe minerals in insoluble residues plays an essential role in Mo enrichment during these stages. During the soil-forming stage of the insoluble residue, most Mo leaches into the water body due to the mineral transformation of insoluble residue. Consequently, as Fe-Mn nodules in soils become more enriched with increasing weathering intensity, some Mo is absorbed and passivated by iron and manganese oxides (hydroxides). Accordingly, the contribution of Fe-Mn nodules and the degree of leaching were closely related to the enrichment of soil Mo in karst regions. This study provides insights into the enrichment mechanisms of Mo in soils developed in karst regions, which will help to evaluate their ecological risk in these environments.

Trichinella pseudospiralis-secreted 53 kDa protein ameliorates imiquimod-induced psoriasis by inhibiting the IL-23/IL-17 axis in mice.

Trichinella infection can experimentally ameliorate many autoimmune diseases. However, the immune mechanism of the amelioration and the identification of corresponding Trichinella-derived molecule(s) are still not fully elucidated. Fifty-three kDa excretory-secretory (ES) protein from Trichinella pseudospiralis (Tpp53) is a molecule like TsP53 reported as a protein exerting immune-inhibitory effect in T. spiralis. In this study, we investigated the immunomodulatory effect of Tpp53 using imiquimod (IMQ)-induced psoriasis-like dermatitis model, which is a mouse model of autoimmune disease with the pathogenic interleukin 17 (IL-17) producing CD4+ T cells (Th17) via IL-23/IL17 axis. Administrating the recombinant Tpp53 (rTpp53) mixed with IMQ cream on the skin of mice ameliorated psoriatic lesions, as revealed by the improvement of erythema, scaling, skin thickening, epidermis hyperplasia and parakeratosis, thickening of acanthosis cell layer, epidermal extension of dermis, less infiltration of inflammatory cells, and decreased expression of inflammatory marker. The increased expression of the factors related to the IL-23/IL-17 axis, including IL-17A, IL-6, Il17F and Il23a, in the skins of IMQ-treated mice was inhibited by rTpp53 treatment. Moreover, the expression of activated keratinocyte-produced cytokines, chemokines, and antimicrobial peptides in the skin was also down-regulated in rTpp53-treated IMQ-treated mice. Co-culture of splenocytes with rTpp53 inhibited IL-17A and treatment of macrophages with rTpp53 reduced IL-6 production. Overall, our study revealed that the Trichinella-secreted 53 kDa ES protein could ameliorate IMQ-induced psoriasis by inhibiting the IL-23/IL-17 axis, suggesting that Tpp53 might involve in regulating host Th17 for immune evasion and have an alternative potential for psoriasis therapy.

Design of Refining Slag Based on Structural Modifications Associated with the Boron Removal for SoG-Si.

Solar grade silicon (SoG-Si) is the core material of solar cells. The removal of boron (B) has always been a challenge in the preparation of high purity Si. Slag refining has always been considered as one of the effective methods to remove B, but the design of refined slag has been limited by the cognition of the relationship between slag structure and impurity removal, and can only rely on the apparent basicity and oxygen potential adjustment of slag based on a large number of conditional experiments. In order to clarify the B removal mechanism of slag refining from Si, nuclear magnetic resonance (NMR) and Raman vibrational spectroscopy were used to investigate in detail the behavior and state of B and aluminum (Al) in the SiO2-CaO-Al2O3-B2O3 slag. The role of the degree of B-Si cross linking on the B activity in slag was highlighted by comparing the partition ratio (LB) between slag and Si. Q2 structural unit of slag is an important site for capturing B. BO4 (1B, 3Si) species is the main form of connection between B and silicate networks, which determines the activity of B in the slag. The addition of Al2O3 into SiO2-CaO slag can change the relative fraction of Q2 and BO4 (1B, 3Si). Increasing Al2O3 content from 0 to about 20 wt% can lead to the overall increase of Q2 population, and a tendency to decrease first and then increase of BO4 (1B, 3Si) fraction under both basicity conditions (0.6 and 1.1). When Al2O3 content is less than 10 ± 1 wt%, the decrease of BO4 (1B, 3Si) population plays a major role in deteriorating the connectivity between B and aluminosilicate network, which leads to a higher activity of B. When the Al2O3 content is greater than 10 ± 1 wt%, B is incorporated into the silicate network more easily due to the formation of more Q2 and BO4 (1B, 3Si), which contributes to a rapid decline in activity of B in slag.

Application of a novel biomimetic double-ligand zirconium-based metal organic framework in environmental restoration and energy conversion.

The development of visible-light response photocatalysts with a high catalytic performance and long-term cyclic stability is of great significance in the field of energy and environmental protection. Inspired by photosynthesis, a novel three-dimensional coral zirconium-based metal organic framework (MOF) was synthesized using a double-ligand strategy. The optimal sample, Zr-TCPP-bpydc (2:1), (the ratio of tetra-(4-carboxyphenyl) porphyrin to 2,2'-bipyridine-5,5'-dicarboxylic acid is 2:1) shows an excellent photocatalytic activity under visible light irradiation, and the effects of the amount of photocatalyst, pH and concentration on the degradation rate were investigated under the optimum conditions. It has a high degradation rate of tetracycline (98.12% for tetracycline and 96.74% for ofloxacin), which is 2.11 times higher than that of single ligand Zr-bpydc (zirconium-based MOF containing only 2,2'-bipyridine-5,5'-dicarboxylic acid). More importantly, it also has a good H2 evolution rate (213.68 µmol g-1h-1) and CO2 reduction (35.81 µmol g-1h-1). In addition, the intermediate pathway of degradation, photocatalytic enhancement mechanism and cycle stability were deeply studied by liquid chromatography-mass spectrometry (LC-MS), electron spin resonance spectroscopy (ESR), linear sweep voltammetry (LSV) and recycling tests. The synthesis of a three-dimensional biomimetic coral zirconium-based MOF material will provide guidance for the development of new, promising, and natural ideal photocatalytic materials.

Sexual Dysfunction in Patients With Urinary Bladder Stones but no Bladder Outlet Obstruction.

Objective: To explore the correlates of sexual dysfunction and lower urinary tract symptoms (LUTS) in male patients with urinary bladder stones and to determine the effect of stone extraction on recovery of sexual function. Materials and Methods: A total of 87 male patients with primary bladder stones were studied from January 2015 to May 2016. All patients underwent pneumatic lithotripsy for bladder stones. Sexual dysfunction was assessed based on sexual function assessment scales. The relationship of bladder stones with sexual dysfunction or LUTS was assessed using a two-sample t-test. Postoperative improvement of sexual function was assessed by repeated measures Analysis of Variance (ANOVA). Results: Forty-one patients had primary bladder stones and 46 had secondary stones from the kidneys. Eighty-three of 87 patients (95%) had sexual dysfunction; 79 patients (91%) had both sexual dysfunction and LUTS. There was a significant association between bladder stones and sexual dysfunction, between sexual dysfunction and LUTS, and between bladder stone and LUTS (p < 0.05). There was no significant association between the course of illness, size and number of bladder stones, or urinary tract infection with sexual function (p > 0.05). In addition, among 83 patients with both bladder stone and sexual dysfunction, 61 patients (73%) had benign prostatic hyperplasia (BPH) and 22 patients (27%) had no BPH. On postoperative evaluation at 3 months, sexual dysfunction scores were significantly improved in 77 patients (88.5%) Conclusion: Patients with bladder stones have a high incidence of sexual dysfunction, particularly those with co-existing LUTS and BPH. About 1/3 patients without BPH had sexual dysfunction and surgical removal of bladder stones significantly improved sexual function and LUTS.

Flower-Like Dual-Defective Z-Scheme Heterojunction g-C3N4/ZnIn2S4 High-Efficiency Photocatalytic Hydrogen Evolution and Degradation of Mixed Pollutants.

Graphitic carbon nitride (g-C3N4) with a porous nano-structure, nitrogen vacancies, and oxygen-doping was prepared by the calcination method. Then, it was combined with ZnIn2S4 nanosheets containing zinc vacancies to construct a three-dimensional (3D) flower-like Z-scheme heterojunction (pCN-N/ZIS-Z), which was used for photocatalytic hydrogen evolution and the degradation of mixed pollutants. The constructed Z-scheme heterojunction improved the efficiency of photogenerated charges separation and migration, and the large surface area and porous characteristics provided more active sites. Doping and defect engineering can change the bandgap structure to improve the utilization of visible light, and can also capture photogenerated electrons to inhibit recombination, so as to promote the use of photogenerated electron-hole pairs in the photocatalytic redox process. Heterojunction and defect engineering synergized to form a continuous and efficient conductive operation framework, which achieves the hydrogen production of pCN-N/ZIS-Z (9189.8 µmol·h-1·g-1) at 58.9 times that of g-C3N4 (155.9 µmol·h-1·g-1), and the degradation rates of methyl orange and metronidazole in the mixed solution were 98.7% and 92.5%, respectively. Our research provides potential ideas for constructing a green and environmentally friendly Z-scheme heterojunction catalyst based on defect engineering to address the energy crisis and environmental restoration.

Early Detection of Trichinella spiralis DNA in Rat Feces Based on Tracing Phosphate Ions Generated During Loop-Mediated Isothermal Amplification.

Early diagnosis of trichinellosis is still difficult because of the lack of specific symptoms and limited window for serological detection. Here we established an assay based on tracing phosphate ions generated during loop-mediated isothermal amplification (LAMP) to detect Trichinella spiralis DNA in rat feces during its early stage of infection. By targeting a 1.6-kb repetitive element of Tri. spiralis, the assay was able to detect Tri. spiralis DNA in the feces of all infected rats as early as 1 day postinfection (dpi). The positive detection lasted to 7 dpi in the rats infected with 250 muscle larvae, and 21 dpi in the rats infected with 5,000 larvae. The assay was highly sensitive, and could detect 1.7 femtograms (fg) of Tri. spiralis DNA with high specificity, and with no cross reactivity with the DNA from Anisakis pegreffii, Gnathostoma spinigerum, Angiostrongylus cantonensis, Enterobius vermicularis, Schistosoma japonicum, and Trypanosoma evansi. Our present study provided a reliable technique for the early diagnosis of trichinellosis with the advantages of simplicity and speed, as well as high sensitivity and specificity.

Glucose transporter Glut1 controls diffuse invasion phenotype with perineuronal satellitosis in diffuse glioma microenvironment.

BACKGROUND: Gliomas typically escape surgical resection and recur due to their "diffuse invasion" phenotype, enabling them to infiltrate diffusely into the normal brain parenchyma. Over the past 80 years, studies have revealed 2 key features of the "diffuse invasion" phenotype, designated the Scherer's secondary structure, and include perineuronal satellitosis (PS) and perivascular satellitosis (PVS). However, the mechanisms are still unknown. METHODS: We established a mouse glioma cell line (IG27) by manipulating the histone H3K27M mutation, frequently harboring in diffuse intrinsic pontine gliomas, that reproduced the diffuse invasion phenotype, PS and PVS, following intracranial transplantation in the mouse brain. Further, to broadly apply the results in this mouse model to human gliomas, we analyzed data from 66 glioma patients. RESULTS: Increased H3K27 acetylation in IG27 cells activated glucose transporter 1 (Glut1) expression and induced aerobic glycolysis and TCA cycle activation, leading to lactate, acetyl-CoA, and oncometabolite production irrespective of oxygen and glucose levels. Gain- and loss-of-function in vivo experiments demonstrated that Glut1 controls the PS of glioma cells, that is, attachment to and contact with neurons. GLUT1 is also associated with early progression in glioma patients. CONCLUSIONS: Targeting the transporter Glut1 suppresses the unique phenotype, "diffuse invasion" in the diffuse glioma mouse model. This work leads to promising therapeutic and potential useful imaging targets for anti-invasion in human gliomas widely.