One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes.

Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types.

[Effect of parotid sparing intensity modulated radiation therapy on salivary composition, flow and dry mouth prevention].

PURPOSE: To investigate the changes of salivary composition, flow and dry mouth in patients with head and neck squamous cell carcinoma after parotid sparing modulated radiation therapy. METHODS: One hundred and one patients with head and neck squamous cell carcinoma who received radiotherapy in Qingyang People's Hospital from May 2016 to November 2018 were selected and divided into intensity modulated radiation group(n=54) and conventional radiation group(n=47) according to different treatment methods. The clinicopathological parameters, parotid uptake index, salivary composition, dry mouth, oropharyngeal symptoms and grading were compared between the two groups. The data were analyzed with SPSS 20.0 software package. RESULTS: The tumor site, TNM stage and differentiation had no significant difference between the two groups(P>0.05). The concentration of total protein, secretory IgA, calcium and phosphorus in saliva of patients in intensity modulated radiation therapy group were significantly higher than those in conventional radiation group(P<0.05). The parotid uptake index, secretion index and salivary flow velocity were significantly decreased in both groups(P<0.05), and were significantly lower in the conventional radiation group than in the intensity modulated radiation group(P<0.05). Dry mouth condition in intensity modulated radiation group was significantly better than that in conventional radiation group(P<0.05). After treatment, the ratio of pharyngalgia and dysphagia patients in the intensity modulated radiation group was significantly lower than that in the conventional radiation group(P<0.05). The degree of oropharyngeal grading in the intensity modulated radiation therapy group was significantly better than that in the conventional radiation group(P<0.05). CONCLUSIONS: The application of parotid sparing retention modulated radiation therapy has little effect on salivary composition, flow rate and dry mouth, and has obvious protective effect on parotid secretion.