RESUMO
Phytochemical investigation of the roots of Scrophularia buergeriana Miq. (Scrophulariaceae), resulted in the isolation of a new iridoid derivative named as buergerinin (1). Its structure was elucidated as rel-(1R, 5R, 6R)-(2-oxa-bicyclo[3.3.0]oct-7-en-6, 7-diyl)dimethoxypropane based mainly on MS and 1D and 2D NMR spectroscopic analyses.
Assuntos
Iridoides/química , Raízes de Plantas/química , Scrophularia/química , Iridoides/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de MassasRESUMO
OBJECTIVE: To detect the concentration of endogenous agmatine in normal and injured rats' spinal cord. METHODS: Forty-two SD rats were randomized to sham-operation group and 1 hour, 4 hours, 8 hours, 24 hours, 48 hours, 72 hours after operation groups. The modified Allen's model of spinal cord injury was established. The endogenous agmatine was detected both in normal and injured rats' spinal cord by high-performance liquid chromatography (HPLC) with fluorescence detection and OPA derivatization. RESULTS: The endogenous agmatine level in naive rats' spinal cord were (0.643 +/- 0.111) microg/g wet weight. After spinal cord injury, the concentration had a transient drop followed by significantly increase, and then decreased by degrees. CONCLUSION: Up to now, little has been known about the endogenous agmatine levels in mammalian tissues. The results reported by different authors varied up to hundred times. According to our results and other articles, the endogenous agmatine levels in mammals' central nervous system arranged from 0.2 microg/g to 1.1 microg/g wet weight. Although there was a significant increase after spinal cord injury, the peak concentration was extremely lower than what it needed to take its neuroprotective effect in vitro.
Assuntos
Agmatina/análise , Traumatismos da Medula Espinal/metabolismo , Medula Espinal/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Modelos Animais de Doenças , Óxido Nítrico Sintase/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-DawleyRESUMO
AIM: To study the chemical constituents of Marsdenia tenacissima (Roxb.) Wight et Arn. METHODS: To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis. RESULTS: Two compounds were isolated from Marsdenia tenacissima and their stuctures were determined as tenacissosides J (I) and tenacissosides K (II). CONCLUSION: Compounds I and II are new C21 steroidal glycosides.
Assuntos
Glicosídeos/isolamento & purificação , Marsdenia/química , Plantas Medicinais/química , Esteroides/isolamento & purificação , Glicosídeos/química , Conformação Molecular , Estrutura Molecular , Caules de Planta/química , Esteroides/químicaRESUMO
Four new pregnane glycosides, named marstenacissides A (1), B (2), C (3), and D (4), have been isolated from the stems of Marsdenia tenacissima. Their structures were established on the basis of chemical and spectral methods.
Assuntos
Glicosídeos/isolamento & purificação , Marsdenia/química , Caules de Planta/química , Pregnanos/química , Glicosídeos/química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
OBJECTIVE: AIM To study the chemical constituents of the fruit of Eucalyptus globulus. METHOD: Various column chromatographies with silica gel were employed for the isolation and purification. The structures of the compounds were elucidated with of spectral analyses and chemical methods. RESULT: Five compounds were isolated and elucidated as follows: betulonic acid (I), betulinic acid (II), ursolic acid (III), corosolic acid (IV), daucosterol (V). CONCLUSION: Compound I, II, III and IV were isolated from this plant for the first time.
